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Entry version 122 (07 Apr 2021)
Sequence version 1 (01 Aug 1998)
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Protein

tRNA-guanine(15) transglycosylase

Gene

tgtA

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Exchanges the guanine residue with 7-cyano-7-deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D-loop) of archaeal tRNAs.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Zn2+2 PublicationsNote: Binds 1 zinc ion per subunit.2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: archaeosine-tRNA biosynthesis

This protein is involved in the pathway archaeosine-tRNA biosynthesis, which is part of tRNA modification.
View all proteins of this organism that are known to be involved in the pathway archaeosine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei95Nucleophile1 Publication1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei130Substrate1 Publication1
Binding sitei196Substrate; via amide nitrogen1 Publication1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi279Zinc2 Publications1
Metal bindingi281Zinc2 Publications1
Metal bindingi284Zinc2 Publications1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processtRNA processing
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
PHOR70601:G1G39-1078-MONOMER

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
2.4.2.48, 5244

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00393

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
tRNA-guanine(15) transglycosylase (EC:2.4.2.48)
Alternative name(s):
7-cyano-7-deazaguanine tRNA-ribosyltransferase
Archaeal tRNA-guanine transglycosylase
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:tgtA
Ordered Locus Names:PH1116
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri70601 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000752 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi95D → A: Abolishes the transferase activity. 1 Publication1
Mutagenesisi96S → A: Weak decrease in transferase activity. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002476171 – 582tRNA-guanine(15) transglycosylaseAdd BLAST582

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer.

2 Publications

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
70601.3257532

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1582
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
O58843

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
O58843

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini507 – 582PUAAdd BLAST76

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
arCOG00989, Archaea
arCOG00991, Archaea

Identification of Orthologs from Complete Genome Data

More...
OMAi
FPCSCPV

Database of Orthologous Groups

More...
OrthoDBi
10236at2157

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.30.130.10, 1 hit
3.10.450.90, 1 hit
3.20.20.105, 1 hit
3.90.1020.10, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_01634, TgtA_arch, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR002478, PUA
IPR015947, PUA-like_sf
IPR036974, PUA_sf
IPR036511, TGT-like_sf
IPR032729, TGT_C1
IPR038370, TGT_C1_sf
IPR029402, TGT_C2
IPR038250, TGT_C2_sf
IPR004804, TgtA
IPR002616, tRNA_ribo_trans-like
IPR004521, Uncharacterised_CHP00451

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01472, PUA, 1 hit
PF01702, TGT, 1 hit
PF14809, TGT_C1, 1 hit
PF14810, TGT_C2, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00359, PUA, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF51713, SSF51713, 1 hit
SSF88697, SSF88697, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00432, arcsn_tRNA_tgt, 1 hit
TIGR00449, tgt_general, 1 hit
TIGR00451, unchar_dom_2, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50890, PUA, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

O58843-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSRGDKMLKF EIKARDGAGR IGKLEVNGKK IETPAIMPVV NPKQMVVEPK
60 70 80 90 100
ELEKMGFEII ITNSYIIYKD EELRRKALEL GIHRMLDYNG IIEVDSGSFQ
110 120 130 140 150
LMKYGSIEVS NREIIEFQHR IGVDIGTFLD IPTPPDAPRE QAVKELEITL
160 170 180 190 200
SRAREAEEIK EIPMNATIQG STYTDLRRYA ARRLSSMNFE IHPIGGVVPL
210 220 230 240 250
LESYRFRDVV DIVISSKMAL RPDRPVHLFG AGHPIVFALA VAMGVDLFDS
260 270 280 290 300
ASYALYAKDD RYMTPEGTKR LDELDYFPCS CPVCSKYTPQ ELREMPKEER
310 320 330 340 350
TRLLALHNLW VIKEEIKRVK QAIKEGELWR LVDERARSHP KLYSAYKRLL
360 370 380 390 400
EHYTFLEEFE PITKKSALFK ISNESLRWPV VRRAKERAKS INERFGELVE
410 420 430 440 450
HPIFGRVSRY LSLTYPFAQS EAEDDFKIEK PTKEDAIKYV MAIAEYQFGE
460 470 480 490 500
GASRAFDDAK VELSKTGMPR QVKVNGKRLA TVRADDGLLT LGIEGAKRLH
510 520 530 540 550
RVLPYPRMRV VVNKEAEPFA RKGKDVFAKF VIFADPGIRP YDEVLVVNEN
560 570 580
DELLATGQAL LSGREMIVFQ YGRAVKVRKG VE
Length:582
Mass (Da):66,596
Last modified:August 1, 1998 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iB96F1D5EC0D73AC3
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA30215.1

Protein sequence database of the Protein Information Resource

More...
PIRi
E71052

NCBI Reference Sequences

More...
RefSeqi
WP_010885200.1, NC_000961.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
BAA30215; BAA30215; BAA30215

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
1443435

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
pho:PH1116

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA30215.1
PIRiE71052
RefSeqiWP_010885200.1, NC_000961.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IQ8X-ray2.20A/B1-582[»]
1IT7X-ray2.30A/B1-582[»]
1IT8X-ray2.50A/B1-582[»]
1J2BX-ray3.30A/B1-582[»]
SMRiO58843
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

STRINGi70601.3257532

Genome annotation databases

EnsemblBacteriaiBAA30215; BAA30215; BAA30215
GeneIDi1443435
KEGGipho:PH1116

Phylogenomic databases

eggNOGiarCOG00989, Archaea
arCOG00991, Archaea
OMAiFPCSCPV
OrthoDBi10236at2157

Enzyme and pathway databases

UniPathwayiUPA00393
BioCyciPHOR70601:G1G39-1078-MONOMER
BRENDAi2.4.2.48, 5244

Miscellaneous databases

EvolutionaryTraceiO58843

Family and domain databases

Gene3Di2.30.130.10, 1 hit
3.10.450.90, 1 hit
3.20.20.105, 1 hit
3.90.1020.10, 1 hit
HAMAPiMF_01634, TgtA_arch, 1 hit
InterProiView protein in InterPro
IPR002478, PUA
IPR015947, PUA-like_sf
IPR036974, PUA_sf
IPR036511, TGT-like_sf
IPR032729, TGT_C1
IPR038370, TGT_C1_sf
IPR029402, TGT_C2
IPR038250, TGT_C2_sf
IPR004804, TgtA
IPR002616, tRNA_ribo_trans-like
IPR004521, Uncharacterised_CHP00451
PfamiView protein in Pfam
PF01472, PUA, 1 hit
PF01702, TGT, 1 hit
PF14809, TGT_C1, 1 hit
PF14810, TGT_C2, 1 hit
SMARTiView protein in SMART
SM00359, PUA, 1 hit
SUPFAMiSSF51713, SSF51713, 1 hit
SSF88697, SSF88697, 1 hit
TIGRFAMsiTIGR00432, arcsn_tRNA_tgt, 1 hit
TIGR00449, tgt_general, 1 hit
TIGR00451, unchar_dom_2, 1 hit
PROSITEiView protein in PROSITE
PS50890, PUA, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiATGT_PYRHO
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O58843
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: August 1, 1998
Last modified: April 7, 2021
This is version 122 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
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