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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Phosphoribosylamine--glycine ligase (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei10NucleophileUniRule annotation1
Metal bindingi239Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi286MagnesiumUniRule annotation1
Metal bindingi346MagnesiumUniRule annotation1
Metal bindingi347MagnesiumUniRule annotation1
Metal bindingi383Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi432Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi435Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124

Protein family/group databases

MEROPSiC44.001

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Short name:
GPATaseUniRule annotation
Gene namesi
Name:purFUniRule annotation
Ordered Locus Names:PH0240
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000292791 – 9Curated9
ChainiPRO_000002928010 – 449AmidophosphoribosyltransferaseAdd BLAST440

Proteomic databases

PRIDEiO57979

Interactioni

Protein-protein interaction databases

IntActiO57979, 1 interactor
MINTiO57979
STRINGi70601.PH0240

Structurei

3D structure databases

ProteinModelPortaliO57979
SMRiO57979
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 224Glutamine amidotransferase type-2UniRule annotationAdd BLAST215

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiarCOG00093 Archaea
COG0034 LUCA
HOGENOMiHOG000033687
KOiK00764
OMAiFRPLCLG
OrthoDBiPOG093Z01WR

Family and domain databases

CDDicd00715 GPATase_N, 1 hit
cd06223 PRTases_typeI, 1 hit
Gene3Di3.60.20.10, 3 hits
HAMAPiMF_01931 PurF, 1 hit
InterProiView protein in InterPro
IPR017932 GATase_2_dom
IPR029055 Ntn_hydrolases_N
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
IPR005854 PurF
IPR035584 PurF_N
PfamiView protein in Pfam
PF13537 GATase_7, 1 hit
PF00156 Pribosyltran, 1 hit
PIRSFiPIRSF000485 Amd_phspho_trans, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
SSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR01134 purF, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit
PS00103 PUR_PYR_PR_TRANSFER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O57979-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGEIMKEKC GIFGAYSQDA TKKTYYGLMA LQHRGQEGAG ISVWDGDIRT
60 70 80 90 100
VKGHGLVSEV FKGGSIRRLN GNPVIGHVRY STSGSLSEVQ PLEVECCGYK
110 120 130 140 150
VSIAHNGTLT NFLPLRRFYE SRGFKFRSSI DTEVIAVSFL NHYSELKDEF
160 170 180 190 200
EAMSRVFEEV KGAYSVLMLF NGKLIAVRDP VGFRPLSFGA GDGYYFSSED
210 220 230 240 250
SALRMFCTNI RDVSPGEVIV VKDGEAESKI VGRSEHAYCV FEYIYFARPD
260 270 280 290 300
SIINGISVYW ARYRMGVELA RESPAEGDVV IAVPDSGRTA ALGFAHESGI
310 320 330 340 350
PYMEGLIKNR YIGRTFIMPS GREIKVRLKL SPVKEVIKGR RIVLVDDSIV
360 370 380 390 400
RGTTMKNIVK MLRDAGAREV HVRIASPPIR YPCYMGIDIP TRHELIAAWK
410 420 430 440
SIEEIKKEIG ADSLAYLSVE GLKRAIGTDK LCMACLTGNY PEWAFDFKV
Length:449
Mass (Da):50,052
Last modified:August 1, 1998 - v1
Checksum:iBA9E98A9FA1713A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA29312.1
PIRiA71248

Genome annotation databases

EnsemblBacteriaiBAA29312; BAA29312; BAA29312
KEGGipho:PH0240

Similar proteinsi

Entry informationi

Entry nameiPUR1_PYRHO
AccessioniPrimary (citable) accession number: O57979
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: June 20, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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