UniProtKB - O57934 (PRIS_PYRHO)
Protein
DNA primase small subunit PriS
Gene
priS
Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Functioni
Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.UniRule annotation1 Publication
Miscellaneous
The bound zinc ion is not a cofactor. It is bound to a zinc knuckle motif that may be involved in sequence recognition and the binding of ssDNA (PubMed:14750947).1 Publication
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 95 | UniRule annotation1 Publication | 1 | |
Active sitei | 97 | UniRule annotation1 Publication | 1 | |
Metal bindingi | 106 | Zinc1 Publication | 1 | |
Metal bindingi | 108 | Zinc; via tele nitrogen1 Publication | 1 | |
Metal bindingi | 114 | Zinc1 Publication | 1 | |
Metal bindingi | 117 | Zinc1 Publication | 1 | |
Active sitei | 280 | UniRule annotation1 Publication | 1 |
GO - Molecular functioni
- DNA primase activity Source: UniProtKB-UniRule
- metal ion binding Source: UniProtKB-KW
Keywordsi
Molecular function | Nucleotidyltransferase, Transferase |
Biological process | DNA replication, Transcription |
Ligand | Magnesium, Manganese, Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | PHOR70601:G1G39-185-MONOMER |
BRENDAi | 2.7.7.B16, 5244 |
Names & Taxonomyi
Protein namesi | Recommended name: DNA primase small subunit PriSUniRule annotation (EC:2.7.7.-UniRule annotation)Alternative name(s): DNA primase 41 kDa subunit Short name: p41 |
Gene namesi | Name:priSUniRule annotation Synonyms:priA Ordered Locus Names:PH0195 |
Organismi | Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) |
Taxonomic identifieri | 70601 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › |
Proteomesi |
|
Subcellular locationi
Other locations
- primosome complex Source: UniProtKB-KW
Keywords - Cellular componenti
DNA-directed RNA polymerase, PrimosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046751 | 1 – 346 | DNA primase small subunit PriSAdd BLAST | 346 |
Interactioni
Subunit structurei
Heterodimer of a small subunit (PriS) and a large subunit (PriL).
UniRule annotation2 PublicationsProtein-protein interaction databases
STRINGi | 70601.3256581 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O57934 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O57934 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 106 – 117 | Zinc knuckle motifAdd BLAST | 12 |
Sequence similaritiesi
Belongs to the eukaryotic-type primase small subunit family.UniRule annotation
Phylogenomic databases
eggNOGi | arCOG04110, Archaea |
OMAi | GFDHTGE |
OrthoDBi | 95578at2157 |
Family and domain databases
CDDi | cd04860, AE_Prim_S, 1 hit |
HAMAPi | MF_00700, DNA_primase_sml_arc, 1 hit |
InterProi | View protein in InterPro IPR002755, DNA_primase_S IPR014052, DNA_primase_ssu_euk/arc IPR023639, DNA_primase_ssu_PriS |
PANTHERi | PTHR10536, PTHR10536, 1 hit |
Pfami | View protein in Pfam PF01896, DNA_primase_S, 1 hit |
TIGRFAMsi | TIGR00335, primase_sml, 1 hit |
i Sequence
Sequence statusi: Complete.
O57934-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLLREVTREE RKNFYTNEWK VKDIPDFIVK TLELREFGFD HSGEGPSDRK
60 70 80 90 100
NQYTDIRDLE DYIRATAPYA VYSSVALYEK PQEMEGWLGT ELVFDIDAKD
110 120 130 140 150
LPLRRCEHEP GTVCPICLND AKEIVRDTVI ILREELGFND IHIIYSGRGY
160 170 180 190 200
HIRVLDEWAL KLDSKSRERI LSFVSASEIE DVEEFRKLLL NKRGWFVLNH
210 220 230 240 250
GYPRAFRLRF GYFILRIKLP HLINAGIRKS IAKSILKSKE EIYEEFVRKA
260 270 280 290 300
ILAAFPQGVG IESLAKLFAL STRFSKSYFD GRVTVDLKRI LRLPSTLHSK
310 320 330 340
VGLIAKYVGT NERDVMRFNP FKHAVPKFRK EEVKVEYKKF LESLGT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000001 Genomic DNA Translation: BAA29264.1 |
PIRi | A71242 |
RefSeqi | WP_010884304.1, NC_000961.1 |
Genome annotation databases
EnsemblBacteriai | BAA29264; BAA29264; BAA29264 |
GeneIDi | 1444086 |
KEGGi | pho:PH0195 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000001 Genomic DNA Translation: BAA29264.1 |
PIRi | A71242 |
RefSeqi | WP_010884304.1, NC_000961.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1V33 | X-ray | 1.80 | A | 1-346 | [»] | |
1V34 | X-ray | 2.70 | A | 1-346 | [»] | |
SMRi | O57934 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 70601.3256581 |
Genome annotation databases
EnsemblBacteriai | BAA29264; BAA29264; BAA29264 |
GeneIDi | 1444086 |
KEGGi | pho:PH0195 |
Phylogenomic databases
eggNOGi | arCOG04110, Archaea |
OMAi | GFDHTGE |
OrthoDBi | 95578at2157 |
Enzyme and pathway databases
BioCyci | PHOR70601:G1G39-185-MONOMER |
BRENDAi | 2.7.7.B16, 5244 |
Miscellaneous databases
EvolutionaryTracei | O57934 |
Family and domain databases
CDDi | cd04860, AE_Prim_S, 1 hit |
HAMAPi | MF_00700, DNA_primase_sml_arc, 1 hit |
InterProi | View protein in InterPro IPR002755, DNA_primase_S IPR014052, DNA_primase_ssu_euk/arc IPR023639, DNA_primase_ssu_PriS |
PANTHERi | PTHR10536, PTHR10536, 1 hit |
Pfami | View protein in Pfam PF01896, DNA_primase_S, 1 hit |
TIGRFAMsi | TIGR00335, primase_sml, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRIS_PYRHO | |
Accessioni | O57934Primary (citable) accession number: O57934 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | August 1, 1998 | |
Last modified: | April 7, 2021 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families