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Protein

Heat shock protein HSP 90-beta

Gene

hsp90ab1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle (By similarity). Not required for myofibril formation in skeletal muscles (PubMed:10364427, PubMed:17586488).By similarity2 Publications

Activity regulationi

In the resting state, through the dimerization of its C-terminal domain, HSP90 forms a homodimer which is defined as the open conformation. Upon ATP-binding, the N-terminal domain undergoes significant conformational changes and comes in contact to form an active closed conformation. After HSP90 finishes its chaperoning tasks of assisting the proper folding, stabilization and activation of client proteins under the active state, ATP molecule is hydrolyzed to ADP which then dissociates from HSP90 and directs the protein back to the resting state.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei45ATPBy similarity1
Binding sitei87ATPBy similarity1
Binding sitei106ATPBy similarity1
Binding sitei132ATP; via amide nitrogenBy similarity1
Binding sitei391ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processMyogenesis, Stress response
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock protein HSP 90-beta
Gene namesi
Name:hsp90ab1
Synonyms:hsp90b
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 20

Organism-specific databases

ZFINiZDB-GENE-990415-95 hsp90ab1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000629251 – 725Heat shock protein HSP 90-betaAdd BLAST725

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei225PhosphoserineBy similarity1
Modified residuei254PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO57521
PRIDEiO57521

Expressioni

Tissue specificityi

Detected throughout the embryo and in low levels in the musculature. Expressed predominantly in the developing brain, tail bud and cells surrounding the posterior margin of the yolk tube.2 Publications

Developmental stagei

Expressed throughout embryonic development.1 Publication

Inductioni

By heat shock during early embryogenesis.1 Publication

Gene expression databases

BgeeiENSDARG00000029150 Expressed in 30 organ(s), highest expression level in embryo
ExpressionAtlasiO57521 baseline and differential

Interactioni

Subunit structurei

Monomer. Homodimer (By similarity). Interacts with unc45b (PubMed:17586488).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi78741, 1 interactor
IntActiO57521, 1 interactor
MINTiO57521
STRINGi7955.ENSDARP00000014978

Structurei

3D structure databases

ProteinModelPortaliO57521
SMRiO57521
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi721 – 725TPR repeat-binding5

Domaini

The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.By similarity

Sequence similaritiesi

Belongs to the heat shock protein 90 family.Curated

Phylogenomic databases

eggNOGiKOG0019 Eukaryota
KOG0020 Eukaryota
COG0326 LUCA
GeneTreeiENSGT00920000149061
HOGENOMiHOG000031988
HOVERGENiHBG007374
InParanoidiO57521
KOiK04079
OMAiLRYHSSQ
OrthoDBiEOG091G0270
PhylomeDBiO57521
TreeFamiTF300686

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
Gene3Di1.20.120.790, 1 hit
3.30.565.10, 1 hit
HAMAPiMF_00505 HSP90, 1 hit
InterProiView protein in InterPro
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR019805 Heat_shock_protein_90_CS
IPR037196 HSP90_C
IPR001404 Hsp90_fam
IPR020575 Hsp90_N
IPR020568 Ribosomal_S5_D2-typ_fold
PANTHERiPTHR11528 PTHR11528, 1 hit
PfamiView protein in Pfam
PF02518 HATPase_c, 1 hit
PF00183 HSP90, 1 hit
PIRSFiPIRSF002583 Hsp90, 1 hit
PRINTSiPR00775 HEATSHOCK90
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SUPFAMiSSF110942 SSF110942, 1 hit
SSF54211 SSF54211, 1 hit
SSF55874 SSF55874, 1 hit
PROSITEiView protein in PROSITE
PS00298 HSP90, 1 hit

Sequencei

Sequence statusi: Complete.

O57521-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPEEMRQEEE AETFAFQAEI AQLMSLIINT FYSNKEIFLR ELVSNASDAL
60 70 80 90 100
DKIRYESLTD PTKLDSGKDL KIDIIPNVQE RTLTLIDTGI GMTKADLINN
110 120 130 140 150
LGTIAKSGTK AFMEALQAGA DISMIGQFGV GFYSAYLVAE KVTVITKHND
160 170 180 190 200
DEQYAWESSA GGSFTVKVDH GEPIGRGTKV ILHLKEDQTE YIEEKRVKEV
210 220 230 240 250
VKKHSQFIGY PITLYVEKER DKEISDDEAE EEKAEKEEKE EEGEDKPKIE
260 270 280 290 300
DVGSDDEEDT KDKDKKKKKK IKEKYIDQEE LNKTKPIWTR NPDDISNEEY
310 320 330 340 350
GEFYKSLTND WEDHLAVKHF SVEGQLEFRA LLFIPRRAPF DLFENKKKKN
360 370 380 390 400
NIKLYVRRVF IMDNCEELIP EYLNFIRGVV DSEDLPLNIS REMLQQSKIL
410 420 430 440 450
KVIRKNIVKK CLELFAELAE DKDNYKKFYD AFSKNLKLGI HEDSQNRKKL
460 470 480 490 500
SELLRYQSSQ SGDEMTSLTE YVSRMKENQK SIYYITGESK DQVAHSAFVE
510 520 530 540 550
RVCKRGFEVL YMTEPIDEYC VQQLKDFDGK SLVSVTKEGL ELPEDEDEKK
560 570 580 590 600
KMEEDKAKFE NLCKLMKEIL DKKVEKVTVS NRLVSSPCCI VTSTYGWTAN
610 620 630 640 650
MERIMKAQAL RDNSTMGYMM AKKHLEINPD HPIMETLRQK AEADKNDKAV
660 670 680 690 700
KDLVILLFET ALLSSGFSLD DPQTHSNRIY RMIKLGLGID EDEDVPVEEP
710 720
SSAAAPEDIP PLEGDDDASR MEEVD
Length:725
Mass (Da):83,357
Last modified:May 30, 2006 - v2
Checksum:i97FACE5422684DF9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti98I → M in AAA97519 (PubMed:7980538).Curated1
Sequence conflicti417 – 418EL → DV in AAC21566 (PubMed:10364427).Curated2
Sequence conflicti444S → C in AAB96969 (Ref. 3) Curated1
Sequence conflicti448K → R in AAC21566 (PubMed:10364427).Curated1
Sequence conflicti463D → Y in AAC21566 (PubMed:10364427).Curated1
Sequence conflicti622K → N in AAB96969 (Ref. 3) Curated1
Sequence conflicti647D → T in AAB96969 (Ref. 3) Curated1
Sequence conflicti701S → T in AAB96969 (Ref. 3) Curated1
Sequence conflicti703Missing in AAC21566 (PubMed:10364427).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF068771 mRNA Translation: AAC21566.1
AF042108 mRNA Translation: AAB96969.1
BC065359 mRNA Translation: AAH65359.1
L35587 mRNA Translation: AAA97519.1
RefSeqiNP_571385.2, NM_131310.3
UniGeneiDr.35688

Genome annotation databases

EnsembliENSDART00000020084; ENSDARP00000014978; ENSDARG00000029150
ENSDART00000169321; ENSDARP00000137112; ENSDARG00000029150
GeneIDi30573
KEGGidre:30573

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF068771 mRNA Translation: AAC21566.1
AF042108 mRNA Translation: AAB96969.1
BC065359 mRNA Translation: AAH65359.1
L35587 mRNA Translation: AAA97519.1
RefSeqiNP_571385.2, NM_131310.3
UniGeneiDr.35688

3D structure databases

ProteinModelPortaliO57521
SMRiO57521
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi78741, 1 interactor
IntActiO57521, 1 interactor
MINTiO57521
STRINGi7955.ENSDARP00000014978

Proteomic databases

PaxDbiO57521
PRIDEiO57521

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000020084; ENSDARP00000014978; ENSDARG00000029150
ENSDART00000169321; ENSDARP00000137112; ENSDARG00000029150
GeneIDi30573
KEGGidre:30573

Organism-specific databases

CTDi3326
ZFINiZDB-GENE-990415-95 hsp90ab1

Phylogenomic databases

eggNOGiKOG0019 Eukaryota
KOG0020 Eukaryota
COG0326 LUCA
GeneTreeiENSGT00920000149061
HOGENOMiHOG000031988
HOVERGENiHBG007374
InParanoidiO57521
KOiK04079
OMAiLRYHSSQ
OrthoDBiEOG091G0270
PhylomeDBiO57521
TreeFamiTF300686

Miscellaneous databases

PROiPR:O57521

Gene expression databases

BgeeiENSDARG00000029150 Expressed in 30 organ(s), highest expression level in embryo
ExpressionAtlasiO57521 baseline and differential

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
Gene3Di1.20.120.790, 1 hit
3.30.565.10, 1 hit
HAMAPiMF_00505 HSP90, 1 hit
InterProiView protein in InterPro
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR019805 Heat_shock_protein_90_CS
IPR037196 HSP90_C
IPR001404 Hsp90_fam
IPR020575 Hsp90_N
IPR020568 Ribosomal_S5_D2-typ_fold
PANTHERiPTHR11528 PTHR11528, 1 hit
PfamiView protein in Pfam
PF02518 HATPase_c, 1 hit
PF00183 HSP90, 1 hit
PIRSFiPIRSF002583 Hsp90, 1 hit
PRINTSiPR00775 HEATSHOCK90
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SUPFAMiSSF110942 SSF110942, 1 hit
SSF54211 SSF54211, 1 hit
SSF55874 SSF55874, 1 hit
PROSITEiView protein in PROSITE
PS00298 HSP90, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHS90B_DANRE
AccessioniPrimary (citable) accession number: O57521
Secondary accession number(s): Q6P0Y9, Q90475
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: May 30, 2006
Last modified: October 10, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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