UniProtKB - O57314 (DHB12_ANAPL)
Very-long-chain 3-oxoacyl-CoA reductase
HSD17B12
Functioni
Catalyzes the second of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme has a 3-ketoacyl-CoA reductase activity, reducing 3-ketoacyl-CoA to 3-hydroxyacyl-CoA, within each cycle of fatty acid elongation. Thereby, it may participate in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May also catalyze the transformation of estrone (E1) into estradiol (E2) and play a role in estrogen formation.
By similarityCatalytic activityi
- a very-long-chain (3R)-3-hydroxyacyl-CoA + NADP+ = a very-long-chain 3-oxoacyl-CoA + H+ + NADPHBy similarityEC:1.1.1.330By similarity
- EC:1.1.1.62By similarity
- EC:1.1.1.62By similarity
- (8Z,11Z,14Z)-3-oxoeicosatrienoyl-CoA + H+ + NADPH = (3R)-hydroxy-(8Z,11Z,14Z)-eicosatrienoyl-CoA + NADP+By similarity
: fatty acid biosynthesis Pathwayi
This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.
Pathwayi: estrogen biosynthesis
This protein is involved in the pathway estrogen biosynthesis, which is part of Steroid biosynthesis.By similarityView all proteins of this organism that are known to be involved in the pathway estrogen biosynthesis and in Steroid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 188 | SubstrateBy similarity | 1 | |
Active sitei | 201 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 48 – 77 | NADPBy similarityAdd BLAST | 30 |
GO - Molecular functioni
- 17-beta-hydroxysteroid dehydrogenase (NAD+) activity Source: RHEA
- 17-beta-hydroxysteroid dehydrogenase (NADP+) activity Source: RHEA
- 3-oxo-arachidoyl-CoA reductase activity Source: UniProtKB-EC
- 3-oxo-behenoyl-CoA reductase activity Source: UniProtKB-EC
- 3-oxo-cerotoyl-CoA reductase activity Source: UniProtKB-EC
- 3-oxo-lignoceroyl-CoA reductase activity Source: UniProtKB-EC
GO - Biological processi
- estrogen biosynthetic process Source: UniProtKB-UniPathway
- fatty acid biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis |
Ligand | NADP |
Enzyme and pathway databases
UniPathwayi | UPA00094 UPA00769 |
Names & Taxonomyi
Protein namesi | Recommended name: Very-long-chain 3-oxoacyl-CoA reductaseCurated (EC:1.1.1.330By similarity)Alternative name(s): 17-beta-hydroxysteroid dehydrogenase 12By similarity Short name: 17-beta-HSD 12By similarity 3-ketoacyl-CoA reductaseBy similarity Short name: KARBy similarity Estradiol 17-beta-dehydrogenase 12By similarity (EC:1.1.1.62By similarity) |
Gene namesi | Name:HSD17B12 Synonyms:SPM2 |
Organismi | Anas platyrhynchos (Mallard) (Anas boschas) |
Taxonomic identifieri | 8839 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archelosauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galloanserae › Anseriformes › Anatidae › Anatinae › Anas |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 33 – 53 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 181 – 201 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 274 – 294 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000054577 | 1 – 312 | Very-long-chain 3-oxoacyl-CoA reductaseAdd BLAST | 312 |
Expressioni
Tissue specificityi
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
OMAi | ADRDHRE |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
Pfami | View protein in Pfam PF00106, adh_short, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MLPAAGLLWW VGALGALYAA VRGALGLLGA LRVWGIGAGR AALGPGLGAW
60 70 80 90 100
AVVTGATDGI GKAYAKELAK RGMKVALISR SKEKLDQVAG EITEQYGVET
110 120 130 140 150
KVIVADFGER EDIYDRIRAG LEGLEIGVLV NNVGISYSYP EYFIDVPDLD
160 170 180 190 200
KTIDKMININ IMSVCKMTRL VLPGMLERSK GVILNISSAA GMYPTPLLTL
210 220 230 240 250
YSASKAFVDY FSRGLHAEYK SKGIIVQSVM PYYVATKMSK ISKPSFDKPT
260 270 280 290 300
PETYVRAAIG TVGLQSQTNG CLPHAFMGWV FSILPTSTVM NLLMKTNKQI
310
RARFLKKKMK EK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB009304 mRNA Translation: BAA23765.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB009304 mRNA Translation: BAA23765.1 |
3D structure databases
SMRi | O57314 |
ModBasei | Search... |
Phylogenomic databases
OMAi | ADRDHRE |
Enzyme and pathway databases
UniPathwayi | UPA00094 UPA00769 |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
Pfami | View protein in Pfam PF00106, adh_short, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DHB12_ANAPL | |
Accessioni | O57314Primary (citable) accession number: O57314 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | June 1, 1998 | |
Last modified: | September 29, 2021 | |
This is version 98 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families