Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

11-cis retinol dehydrogenase

Gene

Rdh5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Stereospecific 11-cis retinol dehydrogenase, which catalyzes the final step in the biosynthesis of 11-cis retinaldehyde, the universal chromophore of visual pigments. Also able to oxidize 9-cis-retinol and 13-cis-retinol, but not all-trans-retinol. Active in the presence of NAD as cofactor but not in the presence of NADP.2 Publications

Catalytic activityi

11-cis-retinol-[retinal-binding-protein] + NAD+ = 11-cis-retinal-[retinol-binding-protein] + NADH.2 Publications

Activity regulationi

Inhibited by 9-cis-, 13-cis- and all-trans-retinoic acids, with the most potent inhibitor being 13-cis-retinoic acid. Weakly inhibited by oleic acid.1 Publication

Pathwayi: retinol metabolism

This protein is involved in the pathway retinol metabolism, which is part of Cofactor metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway retinol metabolism and in Cofactor metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei163SubstrateBy similarity1
Active sitei175Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi32 – 56NADPBy similarityAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processSensory transduction, Vision
LigandNAD

Enzyme and pathway databases

BRENDAi1.1.1.105 3474
ReactomeiR-MMU-2453902 The canonical retinoid cycle in rods (twilight vision)
R-MMU-5365859 RA biosynthesis pathway
UniPathwayi
UPA00912

Chemistry databases

SwissLipidsiSLP:000000798

Names & Taxonomyi

Protein namesi
Recommended name:
11-cis retinol dehydrogenaseImported (EC:1.1.1.3152 PublicationsImported)
Short name:
11-cis RDHBy similarity
Short name:
11-cis RoDH1 Publication
Alternative name(s):
9-cis retinol dehydrogenaseImported
Cis-retinol dehydrogenase1 Publication
Retinol dehydrogenase 5Imported
Gene namesi
Name:Rdh5Imported
Synonyms:cRDH1 Publication, rdhImported, RDH4Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1201412 Rdh5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant mice develop normally and are fertile. No abnormalities can be found in the retinal structure, rhodopsin content and fundus appearance of their eyes. Mice display a mild visual phenotype of impaired dark adaptation and accumulation of 11-cis- and 13-cis-retinols and 11-cis- and 13-cis-retinyl esters in the eyes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000041963324 – 31811-cis retinol dehydrogenaseSequence analysisAdd BLAST295

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi160N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiO55240
PaxDbiO55240
PeptideAtlasiO55240
PRIDEiO55240

PTM databases

iPTMnetiO55240
PhosphoSitePlusiO55240

Expressioni

Tissue specificityi

Expressed in eye, liver, kidney, brain, intestine, placenta, epididymus and submaxillary gland. In eye, strongly expressed in the retinal pigment epithelium, with lower expression levels detected in the inner segment of the photoreceptor cells and in the outer plexiform layer. In kidney, strong expression detected in the distal tubules and the transitional epithelium in the renal pelvis, with weaker expression detected in the epithelium of the outer stripe of the outer zone of the medulla. In liver, detected in hepatocytes in the centrilobular area. In lung, present in Clara cells in the epithelium of the bronchiole, in parenchyma and in cartilage surrounding the secondary bronchi. In skin, expressed in epidermis, hair follicles and mast cells in the dermis. Expressed in heart (PubMed:10588954 and PubMed:10739682). Not detected in heart (PubMed:9539749). Not detected in lung, spleen, skeletal muscle and testis.4 Publications

Developmental stagei

Abundantly expressed during embryonic development, especially in the developing central nervous system and sensory organs, cranial and spinal ganglia and endoderm of foregut and hindgut. At E10, detected along the entire neural tube, the mid- and hindbrain floor, the central canal of the brain vesicles, spinal cord, lung mesenchyme, the trabecular layer of the heart ventricles, endoderm and endodermally-derived structures such as tracheal epithelium and liver. At E11, expressed in the brain vesicles, along the spinal cord, myotome, migrating muscle progenitor cells in the body wall, cells of the genital ridge, spinal ganglion, liver, cerebellar primordium, basal cells of the neuroepithelium of the mesenchephalic flexure, collections of cells in the pons, Rathke's pouch, spinal and cranial ganglia and the floor plate, retina, lens, optic stalks and the neural crest-derived mesenchyme in the anterior eye segment. During eye development, expression restricted to the retinal pigment epithelium of the posterior hemisphere at E18, with expression levels increasing postnatally to P16.2 Publications

Gene expression databases

BgeeiENSMUSG00000025350 Expressed in 166 organ(s), highest expression level in camera-type eye
ExpressionAtlasiO55240 baseline and differential
GenevisibleiO55240 MM

Interactioni

Protein-protein interaction databases

BioGridi202843, 1 interactor
STRINGi10090.ENSMUSP00000026406

Structurei

3D structure databases

ProteinModelPortaliO55240
SMRiO55240
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1610 Eukaryota
ENOG410Y7FK LUCA
GeneTreeiENSGT00930000150852
HOVERGENiHBG005482
InParanoidiO55240
KOiK00061
OMAiGFFQTPV
OrthoDBiEOG091G0LMI
PhylomeDBiO55240
TreeFamiTF325617

Family and domain databases

InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR002347 SDR_fam
PfamiView protein in Pfam
PF00106 adh_short, 1 hit
PRINTSiPR00081 GDHRDH
PR00080 SDRFAMILY
SUPFAMiSSF51735 SSF51735, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

O55240-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MWLPLLLGAL LWAVLWLLRD RQSLPASDAF IFITGCDSGF GRLLALQLDQ
60 70 80 90 100
KGFQVLAGCL TPSGAEDLQQ MASSRLHTTL LDITDPQNVQ QVAKWVKTRV
110 120 130 140 150
GETGLFGLVN NAGVAGIIGP TPWLTQDDFQ RVLSVNTLGP IGVTLALLPL
160 170 180 190 200
LQQARGRVVN ITSVLGRIAA NGGGYCVSKF GLEAFSDSLR RDMAPFGVQV
210 220 230 240 250
SIVEPGFFRT PVTNLESLES TLKACWARLP PAIQAHYGEA FLDTYLRVQR
260 270 280 290 300
RIMNLICDPE LTKVTSCLEH ALTARHPRTR YSPGWDAKLL WLPASYLPAR
310
VVDAVLTWIL PRPAQSVS
Length:318
Mass (Da):34,826
Last modified:June 1, 1998 - v1
Checksum:iAA8DB76CD0BF7DFC
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YWG0D3YWG0_MOUSE
11-cis retinol dehydrogenase
Rdh5
107Annotation score:
D3YZQ7D3YZQ7_MOUSE
11-cis retinol dehydrogenase
Rdh5
175Annotation score:
F7CWI9F7CWI9_MOUSE
11-cis retinol dehydrogenase
Rdh5
154Annotation score:
D3Z0N7D3Z0N7_MOUSE
11-cis retinol dehydrogenase
Rdh5
144Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97752 Genomic DNA Translation: CAA66347.1
AF013288 mRNA Translation: AAC25951.1
AF033195 mRNA Translation: AAC00491.1
AF033196 mRNA Translation: AAC00492.1
AK135139 mRNA Translation: BAE22435.1
AC122380 Genomic DNA No translation available.
CH466578 Genomic DNA Translation: EDL24632.1
BC021372 mRNA Translation: AAH21372.1
CCDSiCCDS24298.1
RefSeqiNP_598767.1, NM_134006.4
XP_006513450.1, XM_006513387.2
UniGeneiMm.439856

Genome annotation databases

EnsembliENSMUST00000026406; ENSMUSP00000026406; ENSMUSG00000025350
GeneIDi19682
KEGGimmu:19682
UCSCiuc007how.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97752 Genomic DNA Translation: CAA66347.1
AF013288 mRNA Translation: AAC25951.1
AF033195 mRNA Translation: AAC00491.1
AF033196 mRNA Translation: AAC00492.1
AK135139 mRNA Translation: BAE22435.1
AC122380 Genomic DNA No translation available.
CH466578 Genomic DNA Translation: EDL24632.1
BC021372 mRNA Translation: AAH21372.1
CCDSiCCDS24298.1
RefSeqiNP_598767.1, NM_134006.4
XP_006513450.1, XM_006513387.2
UniGeneiMm.439856

3D structure databases

ProteinModelPortaliO55240
SMRiO55240
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202843, 1 interactor
STRINGi10090.ENSMUSP00000026406

Chemistry databases

SwissLipidsiSLP:000000798

PTM databases

iPTMnetiO55240
PhosphoSitePlusiO55240

Proteomic databases

MaxQBiO55240
PaxDbiO55240
PeptideAtlasiO55240
PRIDEiO55240

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026406; ENSMUSP00000026406; ENSMUSG00000025350
GeneIDi19682
KEGGimmu:19682
UCSCiuc007how.1 mouse

Organism-specific databases

CTDi5959
MGIiMGI:1201412 Rdh5

Phylogenomic databases

eggNOGiKOG1610 Eukaryota
ENOG410Y7FK LUCA
GeneTreeiENSGT00930000150852
HOVERGENiHBG005482
InParanoidiO55240
KOiK00061
OMAiGFFQTPV
OrthoDBiEOG091G0LMI
PhylomeDBiO55240
TreeFamiTF325617

Enzyme and pathway databases

UniPathwayi
UPA00912

BRENDAi1.1.1.105 3474
ReactomeiR-MMU-2453902 The canonical retinoid cycle in rods (twilight vision)
R-MMU-5365859 RA biosynthesis pathway

Miscellaneous databases

PROiPR:O55240
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025350 Expressed in 166 organ(s), highest expression level in camera-type eye
ExpressionAtlasiO55240 baseline and differential
GenevisibleiO55240 MM

Family and domain databases

InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR002347 SDR_fam
PfamiView protein in Pfam
PF00106 adh_short, 1 hit
PRINTSiPR00081 GDHRDH
PR00080 SDRFAMILY
SUPFAMiSSF51735 SSF51735, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRDH1_MOUSE
AccessioniPrimary (citable) accession number: O55240
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2012
Last sequence update: June 1, 1998
Last modified: November 7, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again