UniProtKB - O55239 (NNMT_MOUSE)

BLAST

>sp|O55239|NNMT_MOUSE Nicotinamide N-methyltransferase OS=Mus musculus OX=10090 GN=Nnmt PE=1 SV=1
MESGFTSKDTYLSHFNPRDYLEKYYSFGSRHCAENEILRHLLKNLFKIFCLGAVKGELLI
DIGSGPTIYQLLSACESFTEIIVSDYTDQNLWELQKWLKKEPGAFDWSPVVTYVCDLEGN
RMKGPEKEEKLRRAIKQVLKCDVTQSQPLGGVSLPPADCLLSTLCLDAACPDLPAYRTAL
RNLGSLLKPGGFLVMVDALKSSYYMIGEQKFSSLPLGWETVRDAVEEAGYTIEQFEVISQ
NYSSTTSNNEGLFSLVGRKPGRSE

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History
Entry version 129 (07 Nov 2018)
Sequence version 1 (01 Jun 1998)
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Protein

Nicotinamide N-methyltransferase

Gene

Nnmt

Organism

Mus musculus (Mouse)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.

Catalytic activityⁱ

S-adenosyl-L-methionine + nicotinamide = S-adenosyl-L-homocysteine + 1-methylnicotinamide.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	20	S-adenosyl-L-methionine	1
Binding siteⁱ	25	S-adenosyl-L-methionine	1
Binding siteⁱ	69	S-adenosyl-L-methionine	1
Binding siteⁱ	90	S-adenosyl-L-methionine	1
Binding siteⁱ	163	S-adenosyl-L-methionine	1

GO - Molecular functionⁱ

nicotinamide N-methyltransferase activity
Source: MGI
Inferred from direct assayⁱ
- 8845860

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

animal organ regeneration Source: Ensembl
methylation
Source: MGI
Inferred from direct assayⁱ
- 8845860
response to drug Source: Ensembl
response to organonitrogen compound Source: Ensembl

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Methyltransferase, Transferase
Ligand	S-adenosyl-L-methionine

Enzyme and pathway databases

Reactomeⁱ	R-MMU-156581 Methylation R-MMU-197264 Nicotinamide salvaging

Reactomeⁱ

R-MMU-156581 Methylation
R-MMU-197264 Nicotinamide salvaging

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Nicotinamide N-methyltransferase (EC:2.1.1.1)
Gene namesⁱ	Name:Nnmt
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 9

Organism-specific databases

MGIⁱ	MGI:1099443 Nnmt

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000159707	1 – 264	Nicotinamide N-methyltransferaseAdd BLAST		264

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O55239
PaxDbⁱ	O55239
PeptideAtlas More...PeptideAtlasⁱ	O55239
PRIDEⁱ	O55239

PTM databases

iPTMnetⁱ	O55239
PhosphoSitePlusⁱ	O55239

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000032271 Expressed in 146 organ(s), highest expression level in white adipose tissue
CleanExⁱ	MM_NNMT
ExpressionAtlasⁱ	O55239 baseline and differential
Genevisibleⁱ	O55239 MM

Interactionⁱ

Subunit structureⁱ

Monomer.By similarity

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000034808

STRINGⁱ

10090.ENSMUSP00000034808

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	8 – 14	Combined sources	7
Helixⁱ	17 – 25	Combined sources	9
Beta strandⁱ	28 – 30	Combined sources	3
Helixⁱ	32 – 50	Combined sources	19
Beta strandⁱ	51 – 53	Combined sources	3
Beta strandⁱ	56 – 63	Combined sources	8
Helixⁱ	69 – 71	Combined sources	3
Helixⁱ	74 – 76	Combined sources	3
Beta strandⁱ	78 – 86	Combined sources	9
Helixⁱ	88 – 98	Combined sources	11
Helixⁱ	108 – 117	Combined sources	10
Turnⁱ	118 – 120	Combined sources	3
Helixⁱ	124 – 134	Combined sources	11
Beta strandⁱ	135 – 140	Combined sources	6
Beta strandⁱ	145 – 147	Combined sources	3
Turnⁱ	148 – 151	Combined sources	4
Beta strandⁱ	157 – 164	Combined sources	8
Helixⁱ	166 – 169	Combined sources	4
Helixⁱ	173 – 184	Combined sources	12
Beta strandⁱ	187 – 200	Combined sources	14
Beta strandⁱ	203 – 206	Combined sources	4
Beta strandⁱ	209 – 212	Combined sources	4
Helixⁱ	218 – 227	Combined sources	10
Beta strandⁱ	231 – 238	Combined sources	8
Turnⁱ	244 – 246	Combined sources	3
Beta strandⁱ	252 – 258	Combined sources	7

Show more details

3D structure databases

ProteinModelPortalⁱ	O55239
SMRⁱ	O55239
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

O55239

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	63 – 64	S-adenosyl-L-methionine binding	2
Regionⁱ	85 – 87	S-adenosyl-L-methionine binding	3
Regionⁱ	142 – 143	S-adenosyl-L-methionine binding	2

Sequence similaritiesⁱ

Belongs to the class I-like SAM-binding methyltransferase superfamily. NNMT/PNMT/TEMT family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG410IFTZ Eukaryota ENOG41128ZR LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000011708
HOGENOMⁱ	HOG000013229
HOVERGENⁱ	HBG000797
InParanoidⁱ	O55239
KEGG Orthology (KO) More...KOⁱ	K00541
OMAⁱ	EWFEVIS
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0EF7
PhylomeDBⁱ	O55239
TreeFamⁱ	TF313114

Family and domain databases

InterProⁱ	View protein in InterPro IPR025820 NNMT/PNMT/TEMT_CS IPR000940 NNMT_TEMT_trans IPR029063 SAM-dependent_MTases
PANTHERⁱ	PTHR10867 PTHR10867, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01234 NNMT_PNMT_TEMT, 1 hit
PIRSFⁱ	PIRSF000384 PNMTase, 1 hit
SUPFAMⁱ	SSF53335 SSF53335, 1 hit
PROSITEⁱ	View protein in PROSITE PS01100 NNMT_PNMT_TEMT, 1 hit PS51681 SAM_MT_NNMT_PNMT_TEMT, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

O55239-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MESGFTSKDT YLSHFNPRDY LEKYYSFGSR HCAENEILRH LLKNLFKIFC 
        60         70         80         90        100
LGAVKGELLI DIGSGPTIYQ LLSACESFTE IIVSDYTDQN LWELQKWLKK 
       110        120        130        140        150
EPGAFDWSPV VTYVCDLEGN RMKGPEKEEK LRRAIKQVLK CDVTQSQPLG 
       160        170        180        190        200
GVSLPPADCL LSTLCLDAAC PDLPAYRTAL RNLGSLLKPG GFLVMVDALK 
       210        220        230        240        250
SSYYMIGEQK FSSLPLGWET VRDAVEEAGY TIEQFEVISQ NYSSTTSNNE 
       260 
GLFSLVGRKP GRSE

Length:264

Mass (Da):29,598

Last modified:June 1, 1998 - v1

Checksum:ⁱDC3FE92738B7EC90

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	D3YX59	D3YX59_MOUSE		Nicotinamide N-methyltransferase Nicotinamide N-methyltransferase	Nnmt	157	Annotation score:

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U86105 mRNA Translation: AAB94331.1 U86106 mRNA Translation: AAB94332.1 U86108 mRNA Translation: AAB94334.1 U86107 mRNA Translation: AAB94333.1 AF044960, AF029756 Genomic DNA Translation: AAC77360.1 BC028757 mRNA Translation: AAH28757.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS23157.1
NCBI Reference Sequences More...RefSeqⁱ	NP_035054.1, NM_010924.3 XP_011240719.1, XM_011242417.1
UniGeneⁱ	Mm.458542 Mm.8362

Genome annotation databases

Ensemblⁱ	ENSMUST00000034808; ENSMUSP00000034808; ENSMUSG00000032271
GeneIDⁱ	18113
KEGGⁱ	mmu:18113
UCSC genome browser More...UCSCⁱ	uc009pik.1 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O55239	Uncharacterized protein		MOUSE		143	UniRef100_O55239
UPI000B8BB318		MOUSE		284

Protein	Similar proteins		Species	Score	Length	Source
O55239	Uncharacterized protein		MOUSE		143	UniRef90_O55239
Nicotinamide N-methyltransferase		CRIGR		265
nicotinamide N-methyltransferase		MESAU		264
Nicotinamide N-methyltransferase		RAT		264
UPI000A315046		MUSCR		264
+6

Protein	Similar proteins		Species	Score	Length	Source
O55239	Indolethylamine N-methyltransferase	)	HUMAN		263	UniRef50_O95050
Indolethylamine N-methyltransferase		PONAB		263
Indolethylamine N-methyltransferase (Fragment)		PANTR		12
Indolethylamine N-methyltransferase (Fragment)		western gorilla		12
Uncharacterized protein		AOTNA		289
+95

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U86105 mRNA Translation: AAB94331.1 U86106 mRNA Translation: AAB94332.1 U86108 mRNA Translation: AAB94334.1 U86107 mRNA Translation: AAB94333.1 AF044960, AF029756 Genomic DNA Translation: AAC77360.1 BC028757 mRNA Translation: AAH28757.1
CCDSⁱ	CCDS23157.1
RefSeqⁱ	NP_035054.1, NM_010924.3 XP_011240719.1, XM_011242417.1
UniGeneⁱ	Mm.458542 Mm.8362

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2I62	X-ray	1.80	A/B/C/D	1-264	[»]
	5XVK	X-ray	1.88	A/B	1-264	[»]
	5YJI	X-ray	1.99	A/B	1-264	[»]
ProteinModelPortalⁱ	O55239
SMRⁱ	O55239
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000034808

STRINGⁱ

10090.ENSMUSP00000034808

PTM databases

iPTMnetⁱ	O55239
PhosphoSitePlusⁱ	O55239

Proteomic databases

MaxQBⁱ	O55239
PaxDbⁱ	O55239
PeptideAtlasⁱ	O55239
PRIDEⁱ	O55239

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000034808; ENSMUSP00000034808; ENSMUSG00000032271
GeneIDⁱ	18113
KEGGⁱ	mmu:18113
UCSCⁱ	uc009pik.1 mouse

Organism-specific databases

CTDⁱ	4837
MGIⁱ	MGI:1099443 Nnmt

Phylogenomic databases

eggNOGⁱ	ENOG410IFTZ Eukaryota ENOG41128ZR LUCA
GeneTreeⁱ	ENSGT00390000011708
HOGENOMⁱ	HOG000013229
HOVERGENⁱ	HBG000797
InParanoidⁱ	O55239
KOⁱ	K00541
OMAⁱ	EWFEVIS
OrthoDBⁱ	EOG091G0EF7
PhylomeDBⁱ	O55239
TreeFamⁱ	TF313114

Enzyme and pathway databases

Reactomeⁱ	R-MMU-156581 Methylation R-MMU-197264 Nicotinamide salvaging

Reactomeⁱ

R-MMU-156581 Methylation
R-MMU-197264 Nicotinamide salvaging

Miscellaneous databases

EvolutionaryTraceⁱ	O55239
Protein Ontology More...PROⁱ	PR:O55239
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000032271 Expressed in 146 organ(s), highest expression level in white adipose tissue
CleanExⁱ	MM_NNMT
ExpressionAtlasⁱ	O55239 baseline and differential
Genevisibleⁱ	O55239 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR025820 NNMT/PNMT/TEMT_CS IPR000940 NNMT_TEMT_trans IPR029063 SAM-dependent_MTases
PANTHERⁱ	PTHR10867 PTHR10867, 1 hit
Pfamⁱ	View protein in Pfam PF01234 NNMT_PNMT_TEMT, 1 hit
PIRSFⁱ	PIRSF000384 PNMTase, 1 hit
SUPFAMⁱ	SSF53335 SSF53335, 1 hit
PROSITEⁱ	View protein in PROSITE PS01100 NNMT_PNMT_TEMT, 1 hit PS51681 SAM_MT_NNMT_PNMT_TEMT, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NNMT_MOUSE
Accessionⁱ	O55239Primary (citable) accession number: O55239
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
	Last sequence update:	June 1, 1998
	Last modified:	November 7, 2018
	This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

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UniProtKB - O55239 (NNMT_MOUSE)

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Nicotinamide N-methyltransferase

Nnmt

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Cytosol

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ