Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nicotinamide N-methyltransferase

Gene

Nnmt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.

Catalytic activityi

S-adenosyl-L-methionine + nicotinamide = S-adenosyl-L-homocysteine + 1-methylnicotinamide.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei20S-adenosyl-L-methionine1
Binding sitei25S-adenosyl-L-methionine1
Binding sitei69S-adenosyl-L-methionine1
Binding sitei90S-adenosyl-L-methionine1
Binding sitei163S-adenosyl-L-methionine1

GO - Molecular functioni

  • nicotinamide N-methyltransferase activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-MMU-156581 Methylation
R-MMU-197264 Nicotinamide salvaging

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinamide N-methyltransferase (EC:2.1.1.1)
Gene namesi
Name:Nnmt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1099443 Nnmt

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001597071 – 264Nicotinamide N-methyltransferaseAdd BLAST264

Proteomic databases

MaxQBiO55239
PaxDbiO55239
PeptideAtlasiO55239
PRIDEiO55239

PTM databases

iPTMnetiO55239
PhosphoSitePlusiO55239

Expressioni

Gene expression databases

BgeeiENSMUSG00000032271 Expressed in 146 organ(s), highest expression level in white adipose tissue
CleanExiMM_NNMT
ExpressionAtlasiO55239 baseline and differential
GenevisibleiO55239 MM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034808

Structurei

Secondary structure

1264
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO55239
SMRiO55239
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO55239

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni63 – 64S-adenosyl-L-methionine binding2
Regioni85 – 87S-adenosyl-L-methionine binding3
Regioni142 – 143S-adenosyl-L-methionine binding2

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IFTZ Eukaryota
ENOG41128ZR LUCA
GeneTreeiENSGT00390000011708
HOGENOMiHOG000013229
HOVERGENiHBG000797
InParanoidiO55239
KOiK00541
OMAiEWFEVIS
OrthoDBiEOG091G0EF7
PhylomeDBiO55239
TreeFamiTF313114

Family and domain databases

InterProiView protein in InterPro
IPR025820 NNMT/PNMT/TEMT_CS
IPR000940 NNMT_TEMT_trans
IPR029063 SAM-dependent_MTases
PANTHERiPTHR10867 PTHR10867, 1 hit
PfamiView protein in Pfam
PF01234 NNMT_PNMT_TEMT, 1 hit
PIRSFiPIRSF000384 PNMTase, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS01100 NNMT_PNMT_TEMT, 1 hit
PS51681 SAM_MT_NNMT_PNMT_TEMT, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O55239-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MESGFTSKDT YLSHFNPRDY LEKYYSFGSR HCAENEILRH LLKNLFKIFC
60 70 80 90 100
LGAVKGELLI DIGSGPTIYQ LLSACESFTE IIVSDYTDQN LWELQKWLKK
110 120 130 140 150
EPGAFDWSPV VTYVCDLEGN RMKGPEKEEK LRRAIKQVLK CDVTQSQPLG
160 170 180 190 200
GVSLPPADCL LSTLCLDAAC PDLPAYRTAL RNLGSLLKPG GFLVMVDALK
210 220 230 240 250
SSYYMIGEQK FSSLPLGWET VRDAVEEAGY TIEQFEVISQ NYSSTTSNNE
260
GLFSLVGRKP GRSE
Length:264
Mass (Da):29,598
Last modified:June 1, 1998 - v1
Checksum:iDC3FE92738B7EC90
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YX59D3YX59_MOUSE
Nicotinamide N-methyltransferase
Nnmt
157Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86105 mRNA Translation: AAB94331.1
U86106 mRNA Translation: AAB94332.1
U86108 mRNA Translation: AAB94334.1
U86107 mRNA Translation: AAB94333.1
AF044960, AF029756 Genomic DNA Translation: AAC77360.1
BC028757 mRNA Translation: AAH28757.1
CCDSiCCDS23157.1
RefSeqiNP_035054.1, NM_010924.3
XP_011240719.1, XM_011242417.1
UniGeneiMm.458542
Mm.8362

Genome annotation databases

EnsembliENSMUST00000034808; ENSMUSP00000034808; ENSMUSG00000032271
GeneIDi18113
KEGGimmu:18113
UCSCiuc009pik.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86105 mRNA Translation: AAB94331.1
U86106 mRNA Translation: AAB94332.1
U86108 mRNA Translation: AAB94334.1
U86107 mRNA Translation: AAB94333.1
AF044960, AF029756 Genomic DNA Translation: AAC77360.1
BC028757 mRNA Translation: AAH28757.1
CCDSiCCDS23157.1
RefSeqiNP_035054.1, NM_010924.3
XP_011240719.1, XM_011242417.1
UniGeneiMm.458542
Mm.8362

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I62X-ray1.80A/B/C/D1-264[»]
5XVKX-ray1.88A/B1-264[»]
5YJIX-ray1.99A/B1-264[»]
ProteinModelPortaliO55239
SMRiO55239
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034808

PTM databases

iPTMnetiO55239
PhosphoSitePlusiO55239

Proteomic databases

MaxQBiO55239
PaxDbiO55239
PeptideAtlasiO55239
PRIDEiO55239

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034808; ENSMUSP00000034808; ENSMUSG00000032271
GeneIDi18113
KEGGimmu:18113
UCSCiuc009pik.1 mouse

Organism-specific databases

CTDi4837
MGIiMGI:1099443 Nnmt

Phylogenomic databases

eggNOGiENOG410IFTZ Eukaryota
ENOG41128ZR LUCA
GeneTreeiENSGT00390000011708
HOGENOMiHOG000013229
HOVERGENiHBG000797
InParanoidiO55239
KOiK00541
OMAiEWFEVIS
OrthoDBiEOG091G0EF7
PhylomeDBiO55239
TreeFamiTF313114

Enzyme and pathway databases

ReactomeiR-MMU-156581 Methylation
R-MMU-197264 Nicotinamide salvaging

Miscellaneous databases

EvolutionaryTraceiO55239
PROiPR:O55239
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032271 Expressed in 146 organ(s), highest expression level in white adipose tissue
CleanExiMM_NNMT
ExpressionAtlasiO55239 baseline and differential
GenevisibleiO55239 MM

Family and domain databases

InterProiView protein in InterPro
IPR025820 NNMT/PNMT/TEMT_CS
IPR000940 NNMT_TEMT_trans
IPR029063 SAM-dependent_MTases
PANTHERiPTHR10867 PTHR10867, 1 hit
PfamiView protein in Pfam
PF01234 NNMT_PNMT_TEMT, 1 hit
PIRSFiPIRSF000384 PNMTase, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS01100 NNMT_PNMT_TEMT, 1 hit
PS51681 SAM_MT_NNMT_PNMT_TEMT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNNMT_MOUSE
AccessioniPrimary (citable) accession number: O55239
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: June 1, 1998
Last modified: November 7, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again