UniProtKB - O55239 (NNMT_MOUSE)
Protein
Nicotinamide N-methyltransferase
Gene
Nnmt
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.
Catalytic activityi
- EC:2.1.1.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 20 | S-adenosyl-L-methionine | 1 | |
Binding sitei | 25 | S-adenosyl-L-methionine | 1 | |
Binding sitei | 69 | S-adenosyl-L-methionine | 1 | |
Binding sitei | 90 | S-adenosyl-L-methionine | 1 | |
Binding sitei | 163 | S-adenosyl-L-methionine | 1 |
GO - Molecular functioni
- nicotinamide N-methyltransferase activity Source: MGI
- N-methyltransferase activity Source: GO_Central
GO - Biological processi
- animal organ regeneration Source: Ensembl
- methylation Source: MGI
- response to drug Source: Ensembl
- response to organonitrogen compound Source: Ensembl
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
Reactomei | R-MMU-156581, Methylation R-MMU-197264, Nicotinamide salvaging |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Nnmt |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1099443, Nnmt |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000159707 | 1 – 264 | Nicotinamide N-methyltransferaseAdd BLAST | 264 |
Proteomic databases
jPOSTi | O55239 |
MaxQBi | O55239 |
PaxDbi | O55239 |
PeptideAtlasi | O55239 |
PRIDEi | O55239 |
PTM databases
iPTMneti | O55239 |
PhosphoSitePlusi | O55239 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000032271, Expressed in white adipose tissue and 173 other tissues |
ExpressionAtlasi | O55239, baseline and differential |
Genevisiblei | O55239, MM |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000034808 |
Miscellaneous databases
RNActi | O55239, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O55239 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O55239 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 63 – 64 | S-adenosyl-L-methionine binding | 2 | |
Regioni | 85 – 87 | S-adenosyl-L-methionine binding | 3 | |
Regioni | 142 – 143 | S-adenosyl-L-methionine binding | 2 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. NNMT/PNMT/TEMT family.Curated
Phylogenomic databases
eggNOGi | KOG4564, Eukaryota |
GeneTreei | ENSGT00390000011708 |
HOGENOMi | CLU_082526_1_1_1 |
InParanoidi | O55239 |
OMAi | YNFGSRH |
OrthoDBi | 1054662at2759 |
PhylomeDBi | O55239 |
TreeFami | TF313114 |
Family and domain databases
InterProi | View protein in InterPro IPR025820, NNMT/PNMT/TEMT_CS IPR000940, NNMT_TEMT_trans IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR10867, PTHR10867, 1 hit |
Pfami | View protein in Pfam PF01234, NNMT_PNMT_TEMT, 1 hit |
PIRSFi | PIRSF000384, PNMTase, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS01100, NNMT_PNMT_TEMT, 1 hit PS51681, SAM_MT_NNMT_PNMT_TEMT, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O55239-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MESGFTSKDT YLSHFNPRDY LEKYYSFGSR HCAENEILRH LLKNLFKIFC
60 70 80 90 100
LGAVKGELLI DIGSGPTIYQ LLSACESFTE IIVSDYTDQN LWELQKWLKK
110 120 130 140 150
EPGAFDWSPV VTYVCDLEGN RMKGPEKEEK LRRAIKQVLK CDVTQSQPLG
160 170 180 190 200
GVSLPPADCL LSTLCLDAAC PDLPAYRTAL RNLGSLLKPG GFLVMVDALK
210 220 230 240 250
SSYYMIGEQK FSSLPLGWET VRDAVEEAGY TIEQFEVISQ NYSSTTSNNE
260
GLFSLVGRKP GRSE
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD3YX59 | D3YX59_MOUSE | Nicotinamide N-methyltransferase | Nnmt | 157 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U86105 mRNA Translation: AAB94331.1 U86106 mRNA Translation: AAB94332.1 U86108 mRNA Translation: AAB94334.1 U86107 mRNA Translation: AAB94333.1 AF044960, AF029756 Genomic DNA Translation: AAC77360.1 BC028757 mRNA Translation: AAH28757.1 |
CCDSi | CCDS23157.1 |
RefSeqi | NP_035054.1, NM_010924.3 XP_011240719.1, XM_011242417.1 |
Genome annotation databases
Ensembli | ENSMUST00000034808; ENSMUSP00000034808; ENSMUSG00000032271 |
GeneIDi | 18113 |
KEGGi | mmu:18113 |
UCSCi | uc009pik.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U86105 mRNA Translation: AAB94331.1 U86106 mRNA Translation: AAB94332.1 U86108 mRNA Translation: AAB94334.1 U86107 mRNA Translation: AAB94333.1 AF044960, AF029756 Genomic DNA Translation: AAC77360.1 BC028757 mRNA Translation: AAH28757.1 |
CCDSi | CCDS23157.1 |
RefSeqi | NP_035054.1, NM_010924.3 XP_011240719.1, XM_011242417.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2I62 | X-ray | 1.80 | A/B/C/D | 1-264 | [»] | |
5XVK | X-ray | 1.88 | A/B | 1-264 | [»] | |
5YJI | X-ray | 1.99 | A/B | 1-264 | [»] | |
SMRi | O55239 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000034808 |
Chemistry databases
ChEMBLi | CHEMBL4295666 |
PTM databases
iPTMneti | O55239 |
PhosphoSitePlusi | O55239 |
Proteomic databases
jPOSTi | O55239 |
MaxQBi | O55239 |
PaxDbi | O55239 |
PeptideAtlasi | O55239 |
PRIDEi | O55239 |
Protocols and materials databases
Antibodypediai | 18379, 462 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000034808; ENSMUSP00000034808; ENSMUSG00000032271 |
GeneIDi | 18113 |
KEGGi | mmu:18113 |
UCSCi | uc009pik.1, mouse |
Organism-specific databases
CTDi | 4837 |
MGIi | MGI:1099443, Nnmt |
Phylogenomic databases
eggNOGi | KOG4564, Eukaryota |
GeneTreei | ENSGT00390000011708 |
HOGENOMi | CLU_082526_1_1_1 |
InParanoidi | O55239 |
OMAi | YNFGSRH |
OrthoDBi | 1054662at2759 |
PhylomeDBi | O55239 |
TreeFami | TF313114 |
Enzyme and pathway databases
Reactomei | R-MMU-156581, Methylation R-MMU-197264, Nicotinamide salvaging |
Miscellaneous databases
BioGRID-ORCSi | 18113, 0 hits in 17 CRISPR screens |
ChiTaRSi | Nnmt, mouse |
EvolutionaryTracei | O55239 |
PROi | PR:O55239 |
RNActi | O55239, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032271, Expressed in white adipose tissue and 173 other tissues |
ExpressionAtlasi | O55239, baseline and differential |
Genevisiblei | O55239, MM |
Family and domain databases
InterProi | View protein in InterPro IPR025820, NNMT/PNMT/TEMT_CS IPR000940, NNMT_TEMT_trans IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR10867, PTHR10867, 1 hit |
Pfami | View protein in Pfam PF01234, NNMT_PNMT_TEMT, 1 hit |
PIRSFi | PIRSF000384, PNMTase, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS01100, NNMT_PNMT_TEMT, 1 hit PS51681, SAM_MT_NNMT_PNMT_TEMT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NNMT_MOUSE | |
Accessioni | O55239Primary (citable) accession number: O55239 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | June 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families