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Protein

DNA repair protein RAD51 homolog 4

Gene

Rad51d

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Bind to single-stranded DNA (ssDNA) and has DNA-dependent ATPase activity. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Involved in telomere maintenance. The BCDX2 subcomplex XRCC2:RAD51D can stimulate Holliday junction resolution by BLM (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi107 – 114ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: MGI
  • chromosome organization Source: MGI
  • double-strand break repair via homologous recombination Source: UniProtKB
  • interstrand cross-link repair Source: MGI
  • nucleotide-excision repair Source: MGI
  • regulation of cell cycle Source: MGI
  • strand invasion Source: UniProtKB
  • telomere maintenance Source: BHF-UCL
  • telomere maintenance via recombination Source: BHF-UCL

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage, DNA recombination, DNA repair
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5685942 HDR through Homologous Recombination (HRR)
R-MMU-5693568 Resolution of D-loop Structures through Holliday Junction Intermediates
R-MMU-5693579 Homologous DNA Pairing and Strand Exchange
R-MMU-5693616 Presynaptic phase of homologous DNA pairing and strand exchange

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD51 homolog 4
Alternative name(s):
R51H3
RAD51 homolog D
RAD51-like protein 3
Gene namesi
Name:Rad51d
Synonyms:R51h3, Rad51l3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1261809 Rad51d

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

Pathology & Biotechi

Disruption phenotypei

Midgestation lethal.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001229431 – 329DNA repair protein RAD51 homolog 4Add BLAST329

Proteomic databases

EPDiO55230
PaxDbiO55230
PRIDEiO55230

PTM databases

PhosphoSitePlusiO55230

Expressioni

Tissue specificityi

Highly expressed in brain followed by testis. Also expressed in heart, liver, kidney, spleen, lung and skeletal muscle.

Gene expression databases

BgeeiENSMUSG00000018841 Expressed in 256 organ(s), highest expression level in prostate gland
CleanExiMM_RAD51L3
ExpressionAtlasiO55230 baseline and differential
GenevisibleiO55230 MM

Interactioni

Subunit structurei

Part of the BCDX2 complex consisting of RAD51B, RAD51C, RAD51D and XRCC2; the complex has a ring-like structure arranged into a flat disc around a central channel. In the absence of DNA, the BCDX2 subcomplex XRCC2:RAD51D formed a multimeric ring structure; in the presence of single-stranded DNA it formed a filamentous structure with the ssDNA. Interacts with SWSAP1 and ZSWIM7; involved in homologous recombination repair. Interacts with BLM; required for stimulation of BLM activity by the BCDX2 subcomplex XRCC2:RAD51D (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202567, 3 interactors
IntActiO55230, 1 interactor
MINTiO55230
STRINGi10090.ENSMUSP00000018985

Structurei

3D structure databases

ProteinModelPortaliO55230
SMRiO55230
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 83preferentially binds ssDNABy similarityAdd BLAST83
Regioni4 – 77Interaction with XRCC21 PublicationAdd BLAST74
Regioni77 – 328Interaction with RAD51C1 PublicationAdd BLAST252

Sequence similaritiesi

Belongs to the RecA family. RAD51 subfamily.Curated

Phylogenomic databases

eggNOGiKOG1433 Eukaryota
COG0468 LUCA
GeneTreeiENSGT00860000133731
HOGENOMiHOG000049134
HOVERGENiHBG057455
InParanoidiO55230
KOiK10871
OMAiHVTRDRD
OrthoDBiEOG091G0SEJ
PhylomeDBiO55230
TreeFamiTF101219

Family and domain databases

CDDicd01123 Rad51_DMC1_radA, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR013632 DNA_recomb/repair_Rad51_C
IPR016467 DNA_recomb/repair_RecA-like
IPR027417 P-loop_NTPase
IPR033925 Rad51_DMC1_RadA
IPR020588 RecA_ATP-bd
PfamiView protein in Pfam
PF08423 Rad51, 1 hit
PIRSFiPIRSF005856 Rad51, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS50162 RECA_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket
Note: At least 2 isoforms are produced.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O55230-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGMLRAGLCP GLTEETVQLL RGRKIKTVAD LAAADLEEVA QKCGLSYKAL
60 70 80 90 100
VALRRVLLAQ FSAFPLNGAD LYEELKTSTA ILSTGIGSLD KLLDAGLYTG
110 120 130 140 150
EVTEIVGGPG SGKTQVCLCV AANVAHSLQQ NVLYVDSNGG MTASRLLQLL
160 170 180 190 200
QARTQDEEKQ ASALQRIQVV RSFDIFRMLD MLQDLRGTIA QQEATSSGAV
210 220 230 240 250
KVVIVDSVTA VVAPLLGGQQ REGLALMMQL ARELKILARD LGVAVVVTNH
260 270 280 290 300
LTRDWDGRRF KPALGRSWSF VPSTRILLDV TEGAGTLGSS QRTVCLTKSP
310 320
RQPTGLQEMI DIGTLGTEEQ SPELPGKQT
Length:329
Mass (Da):35,260
Last modified:June 1, 1998 - v1
Checksum:i6E463822408EF484
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q9EP85Q9EP85_MOUSE
DNA repair protein RAD51 homolog 4
Rad51d Rad51l3, TRAD/RAD51L3, mCG_8185
275Annotation score:
Q9EP65Q9EP65_MOUSE
DNA repair protein RAD51 homolog 4
Rad51d Rad51l3, TRAD/RAD51L3
53Annotation score:
E9QAG0E9QAG0_MOUSE
DNA repair protein RAD51 homolog 4
Rad51d Rad51l3
233Annotation score:
B1ARD4B1ARD4_MOUSE
DNA repair protein RAD51 homolog 4
Rad51d Rad51l3
284Annotation score:
E9Q428E9Q428_MOUSE
DNA repair protein RAD51 homolog 4
Rad51d Rad51l3
109Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007040 mRNA Translation: BAA24010.1
AF034955 mRNA Translation: AAC40093.1
Y15570 mRNA Translation: CAA75679.1
AK017835 mRNA Translation: BAB30965.1
BC049136 mRNA Translation: AAH49136.1
CCDSiCCDS36250.1 [O55230-1]
RefSeqiNP_035365.1, NM_011235.4 [O55230-1]
UniGeneiMm.441571
Mm.9286

Genome annotation databases

EnsembliENSMUST00000018985; ENSMUSP00000018985; ENSMUSG00000018841 [O55230-1]
GeneIDi19364
KEGGimmu:19364
UCSCiuc007knm.2 mouse [O55230-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007040 mRNA Translation: BAA24010.1
AF034955 mRNA Translation: AAC40093.1
Y15570 mRNA Translation: CAA75679.1
AK017835 mRNA Translation: BAB30965.1
BC049136 mRNA Translation: AAH49136.1
CCDSiCCDS36250.1 [O55230-1]
RefSeqiNP_035365.1, NM_011235.4 [O55230-1]
UniGeneiMm.441571
Mm.9286

3D structure databases

ProteinModelPortaliO55230
SMRiO55230
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202567, 3 interactors
IntActiO55230, 1 interactor
MINTiO55230
STRINGi10090.ENSMUSP00000018985

PTM databases

PhosphoSitePlusiO55230

Proteomic databases

EPDiO55230
PaxDbiO55230
PRIDEiO55230

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018985; ENSMUSP00000018985; ENSMUSG00000018841 [O55230-1]
GeneIDi19364
KEGGimmu:19364
UCSCiuc007knm.2 mouse [O55230-1]

Organism-specific databases

CTDi5892
MGIiMGI:1261809 Rad51d

Phylogenomic databases

eggNOGiKOG1433 Eukaryota
COG0468 LUCA
GeneTreeiENSGT00860000133731
HOGENOMiHOG000049134
HOVERGENiHBG057455
InParanoidiO55230
KOiK10871
OMAiHVTRDRD
OrthoDBiEOG091G0SEJ
PhylomeDBiO55230
TreeFamiTF101219

Enzyme and pathway databases

ReactomeiR-MMU-5685942 HDR through Homologous Recombination (HRR)
R-MMU-5693568 Resolution of D-loop Structures through Holliday Junction Intermediates
R-MMU-5693579 Homologous DNA Pairing and Strand Exchange
R-MMU-5693616 Presynaptic phase of homologous DNA pairing and strand exchange

Miscellaneous databases

PROiPR:O55230
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000018841 Expressed in 256 organ(s), highest expression level in prostate gland
CleanExiMM_RAD51L3
ExpressionAtlasiO55230 baseline and differential
GenevisibleiO55230 MM

Family and domain databases

CDDicd01123 Rad51_DMC1_radA, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR013632 DNA_recomb/repair_Rad51_C
IPR016467 DNA_recomb/repair_RecA-like
IPR027417 P-loop_NTPase
IPR033925 Rad51_DMC1_RadA
IPR020588 RecA_ATP-bd
PfamiView protein in Pfam
PF08423 Rad51, 1 hit
PIRSFiPIRSF005856 Rad51, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS50162 RECA_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRA51D_MOUSE
AccessioniPrimary (citable) accession number: O55230
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: November 7, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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