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Protein

Choline/ethanolamine kinase

Gene

Chkb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a key role in phospholipid biosynthesis. Catalyzes the first step in phosphatidylethanolamine biosynthesis. Phosphorylates ethanolamine, and can also act on choline (in vitro). Has higher activity with ethanolamine. May not significantly contribute to in vivo phosphatidylcholine biosynthesis (By similarity).By similarity

Catalytic activityi

ATP + choline = ADP + phosphocholine.
ATP + ethanolamine = ADP + O-phosphoethanolamine.

Pathwayi: phosphatidylethanolamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes phosphatidylethanolamine from ethanolamine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Choline kinase alpha (Chka), Choline/ethanolamine kinase (Chkb), Ethanolamine kinase 2 (Etnk2), Ethanolamine kinase 1 (Etnk1)
  2. Ethanolamine-phosphate cytidylyltransferase (Pcyt2)
  3. Choline/ethanolaminephosphotransferase 1 (Cept1), Ethanolaminephosphotransferase 1 (Selenoi)
This subpathway is part of the pathway phosphatidylethanolamine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylethanolamine from ethanolamine, the pathway phosphatidylethanolamine biosynthesis and in Phospholipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei104ATPBy similarity1
Binding sitei244ATPBy similarity1
Binding sitei264ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi75 – 81ATPBy similarity7
Nucleotide bindingi146 – 152ATPBy similarity7

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • choline kinase activity Source: MGI
  • ethanolamine kinase activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1483191 Synthesis of PC
R-MMU-1483213 Synthesis of PE
UniPathwayi
UPA00558;UER00741

Names & Taxonomyi

Protein namesi
Recommended name:
Choline/ethanolamine kinase
Alternative name(s):
Choline kinase beta (EC:2.7.1.32)
Short name:
CK
Short name:
CKB
Ethanolamine kinase (EC:2.7.1.82)
Short name:
EK
choline/ethanolamine kinase beta
Short name:
CKEKB
Gene namesi
Name:Chkb
Synonyms:Chetk, Chkl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1328313 Chkb

Pathology & Biotechi

Involvement in diseasei

Defects in Chkb are a cause of rostrocaudal muscular dystrophy (rmd). The disease is characterized by rapidly progressive muscular dystrophy and neonatal forelimb bone deformity. The dystrophy is only evident in skeletal muscle tissues in an unusual rostral-to-caudal gradient.1 Publication

Disruption phenotypei

Hindlimb muscular dystrophy. Hindlimb skeletal muscle tissue exhibits impaired phosphatidylcholine biosynthesis and increased phosphatidylcholine catabolism, with concomitant accumulation of choline. Mitochondria are abnormally large and exhibit decreased inner membrane potential.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002062232 – 394Choline/ethanolamine kinaseAdd BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO55229
PaxDbiO55229
PeptideAtlasiO55229
PRIDEiO55229

PTM databases

iPTMnetiO55229
PhosphoSitePlusiO55229

Expressioni

Tissue specificityi

Expressed ubiquitously with the highest level in testis.

Gene expression databases

BgeeiENSMUSG00000022617 Expressed in 293 organ(s), highest expression level in skeletal muscle tissue
CleanExiMM_CHKB
ExpressionAtlasiO55229 baseline and differential
GenevisibleiO55229 MM

Interactioni

Subunit structurei

Homodimer, and heterodimer with CHKA.1 Publication

Protein-protein interaction databases

IntActiO55229, 1 interactor
STRINGi10090.ENSMUSP00000023289

Structurei

3D structure databases

ProteinModelPortaliO55229
SMRiO55229
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni77 – 79Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the choline/ethanolamine kinase family.Curated

Phylogenomic databases

eggNOGiKOG2686 Eukaryota
COG0510 LUCA
GeneTreeiENSGT00530000062991
HOGENOMiHOG000041274
HOVERGENiHBG050943
InParanoidiO55229
KOiK14156
OMAiMVMPFNK
OrthoDBiEOG091G06UK
PhylomeDBiO55229
TreeFamiTF313549

Family and domain databases

InterProiView protein in InterPro
IPR026712 Cho/Etha_kinase
IPR011009 Kinase-like_dom_sf
PANTHERiPTHR22603:SF35 PTHR22603:SF35, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

O55229-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAADGTGVVG GGAVGGGLPK DGLQDAKCPE PIPNRRRASS LSRDAQRRAY
60 70 80 90 100
QWCREYLGGA WRRARPEELS VCPVSGGLSN LLFRCSLPNH VPSVGGEPRE
110 120 130 140 150
VLLRLYGAIL QGVDSLVLES VMFAILAERS LGPQLYGVFP EGRLEQYLPS
160 170 180 190 200
RPLKTQELRD PVLSGAIATR MARFHGMEMP FTKEPRWLFG TMERYLKQIQ
210 220 230 240 250
DLPSTSLPQM NLVEMYSLKD EMNSLRKLLD DTPSPVVFCH NDIQEGNILL
260 270 280 290 300
LSEPDSDDNL MLVDFEYSSY NYRGFDIGNH FCEWVYDYTY EEWPFYKARP
310 320 330 340 350
TDYPTREQQL HFIRHYLAEV QKGEILSEEE QKKREEELLL EISRYSLASH
360 370 380 390
FFWGLWSTLQ ASMSTIEFGY LEYAQSRFQF YFQQKGQLTS SPSS
Length:394
Mass (Da):45,126
Last modified:January 23, 2007 - v3
Checksum:iA5EE79EC551D07A2
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6SKI6F6SKI6_MOUSE
Choline/ethanolamine kinase
Chkb
164Annotation score:
F6RRT7F6RRT7_MOUSE
Choline/ethanolamine kinase
Chkb
176Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011001 Transcribed RNA Translation: BAA24897.1
AB011000 mRNA Translation: BAA24896.1
CCDSiCCDS27750.1
RefSeqiNP_031718.1, NM_007692.6
UniGeneiMm.485045

Genome annotation databases

EnsembliENSMUST00000023289; ENSMUSP00000023289; ENSMUSG00000022617
GeneIDi12651
KEGGimmu:12651
UCSCiuc007xgs.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011001 Transcribed RNA Translation: BAA24897.1
AB011000 mRNA Translation: BAA24896.1
CCDSiCCDS27750.1
RefSeqiNP_031718.1, NM_007692.6
UniGeneiMm.485045

3D structure databases

ProteinModelPortaliO55229
SMRiO55229
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO55229, 1 interactor
STRINGi10090.ENSMUSP00000023289

PTM databases

iPTMnetiO55229
PhosphoSitePlusiO55229

Proteomic databases

EPDiO55229
PaxDbiO55229
PeptideAtlasiO55229
PRIDEiO55229

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023289; ENSMUSP00000023289; ENSMUSG00000022617
GeneIDi12651
KEGGimmu:12651
UCSCiuc007xgs.2 mouse

Organism-specific databases

CTDi1120
MGIiMGI:1328313 Chkb

Phylogenomic databases

eggNOGiKOG2686 Eukaryota
COG0510 LUCA
GeneTreeiENSGT00530000062991
HOGENOMiHOG000041274
HOVERGENiHBG050943
InParanoidiO55229
KOiK14156
OMAiMVMPFNK
OrthoDBiEOG091G06UK
PhylomeDBiO55229
TreeFamiTF313549

Enzyme and pathway databases

UniPathwayi
UPA00558;UER00741

ReactomeiR-MMU-1483191 Synthesis of PC
R-MMU-1483213 Synthesis of PE

Miscellaneous databases

PROiPR:O55229
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022617 Expressed in 293 organ(s), highest expression level in skeletal muscle tissue
CleanExiMM_CHKB
ExpressionAtlasiO55229 baseline and differential
GenevisibleiO55229 MM

Family and domain databases

InterProiView protein in InterPro
IPR026712 Cho/Etha_kinase
IPR011009 Kinase-like_dom_sf
PANTHERiPTHR22603:SF35 PTHR22603:SF35, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCHKB_MOUSE
AccessioniPrimary (citable) accession number: O55229
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 131 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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