Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Integrin-linked protein kinase

Gene

Ilk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Activity regulationi

Stimulated rapidly but transiently by both cell fibronectin interactions, as well as by insulin, in a PI3-K-dependent manner, likely via the binding of PtdIns(3,4,5)P3 with a PH-like domain of ILK. The protein kinase activity is stimulated by LIMD2.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei220ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi199 – 207ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2 3474
ReactomeiR-MMU-446343 Localization of the PINCH-ILK-PARVIN complex to focal adhesions

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin-linked protein kinase (EC:2.7.11.1)
Gene namesi
Name:Ilk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1195267 Ilk

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000860221 – 452Integrin-linked protein kinaseAdd BLAST452

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei186PhosphoserineBy similarity1
Modified residuei426N6-acetyllysineCombined sources1

Post-translational modificationi

Autophosphorylated on serine residues.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO55222
MaxQBiO55222
PaxDbiO55222
PeptideAtlasiO55222
PRIDEiO55222

PTM databases

iPTMnetiO55222
PhosphoSitePlusiO55222
SwissPalmiO55222

Expressioni

Tissue specificityi

Highly expressed in lung, heart, kidney, liver, brain, spleen and skeletal muscle. Weakly expressed in testis.

Gene expression databases

BgeeiENSMUSG00000030890 Expressed in 280 organ(s), highest expression level in uterus
CleanExiMM_ILK
ExpressionAtlasiO55222 baseline and differential
GenevisibleiO55222 MM

Interactioni

Subunit structurei

Interacts with FERMT2 (PubMed:18483218). Interacts with the cytoplasmic domain of ITGB1 (By similarity). Could also interact with integrin ITGB2, ITGB3 and/or ITGB5 (By similarity). Interacts (via ANK repeats) with LIMS1 and LIMS2 (By similarity). Interacts with PARVA and PARVB; these compete for the same binding site (By similarity). Interacts probably also with TGFB1I1 (PubMed:16737959). Interacts (via ANK repeats) with EPHA1 (via SAM domain); stimulated by EFNA1 but independent of the kinase activity of EPHA1 (By similarity). Interacts with LIMD2; leading to activate the protein kinase activity (By similarity).By similarity2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200649, 9 interactors
CORUMiO55222
DIPiDIP-41971N
IntActiO55222, 48 interactors
MINTiO55222
STRINGi10090.ENSMUSP00000033182

Structurei

3D structure databases

ProteinModelPortaliO55222
SMRiO55222
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati2 – 30ANK 1Add BLAST29
Repeati31 – 63ANK 2Add BLAST33
Repeati64 – 96ANK 3Add BLAST33
Repeati97 – 129ANK 4Add BLAST33
Repeati130 – 174ANK 5Add BLAST45
Domaini193 – 446Protein kinasePROSITE-ProRule annotationAdd BLAST254

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni33 – 139Interaction with LIMS1By similarityAdd BLAST107
Regioni180 – 212PH-like; mediates interaction with TGFB1I11 PublicationAdd BLAST33

Domaini

A PH-like domain is involved in phosphatidylinositol phosphate binding.By similarity

Sequence similaritiesi

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0195 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00930000150972
HOGENOMiHOG000047828
HOVERGENiHBG002437
InParanoidiO55222
KOiK06272
OMAiNMADAKF
OrthoDBiEOG091G05IK
PhylomeDBiO55222
TreeFamiTF315194

Family and domain databases

CDDicd00204 ANK, 1 hit
cd14057 PK_ILK, 1 hit
Gene3Di1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR011009 Kinase-like_dom_sf
IPR035692 PK_ILK
IPR000719 Prot_kinase_dom
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
PfamiView protein in Pfam
PF12796 Ank_2, 2 hits
PF07714 Pkinase_Tyr, 1 hit
SMARTiView protein in SMART
SM00248 ANK, 3 hits
SUPFAMiSSF48403 SSF48403, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 3 hits
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All

O55222-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW ACREGRSAVV
60 70 80 90 100
EMLIMRGARI NVMNRGDDTP LHLAASHGHR DIVQKLLQYK ADINAVNEHG
110 120 130 140 150
NVPLHYACFW GQDQVAEDLV ANGALVSICN KYGEMPVDKA KAPLRELLRE
160 170 180 190 200
RAEKMGQNLN RIPYKDTFWK GTTRTRPRNG TLNKHSGIDF KQLNFLAKLN
210 220 230 240 250
ENHSGELWKG RWQGNDIVVK VLKVRDWSTR KSRDFNEECP RLRIFSHPNV
260 270 280 290 300
LPVLGACQAP PAPHPTLITH WMPYGSLYNV LHEGTNFVVD QSQAVKFALD
310 320 330 340 350
MARGMAFLHT LEPLIPRHAL NSRSVMIDED MTARISMADV KFSFQCPGRM
360 370 380 390 400
YAPAWVAPEA LQKKPEDTNR RSADMWSFAV LLWELVTREV PFADLSNMEI
410 420 430 440 450
GMKVALEGLR PTIPPGISPH VCKLMKICMN EDPAKRPKFD MIVPILEKMQ

DK
Length:452
Mass (Da):51,373
Last modified:October 31, 2006 - v2
Checksum:iF41960CF8EC503A7
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YZA5D3YZA5_MOUSE
Integrin-linked protein kinase
Ilk
295Annotation score:
F6Q5Z1F6Q5Z1_MOUSE
Integrin-linked protein kinase
Ilk
17Annotation score:
A0A1B0GRF6A0A1B0GRF6_MOUSE
Integrin-linked protein kinase
Ilk mCG_19714
233Annotation score:
A0A1B0GR42A0A1B0GR42_MOUSE
Integrin-linked protein kinase
Ilk
95Annotation score:
A0A1B0GRW6A0A1B0GRW6_MOUSE
Integrin-linked protein kinase
Ilk
54Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti93I → T in AAB94646 (PubMed:9366252).Curated1
Sequence conflicti413I → V in AAB94646 (PubMed:9366252).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94479 mRNA Translation: AAB94646.1
BC003737 mRNA Translation: AAH03737.1
CCDSiCCDS21659.1
RefSeqiNP_001155196.1, NM_001161724.1
NP_034692.2, NM_010562.2
UniGeneiMm.274846

Genome annotation databases

EnsembliENSMUST00000033182; ENSMUSP00000033182; ENSMUSG00000030890
ENSMUST00000163389; ENSMUSP00000130341; ENSMUSG00000030890
GeneIDi16202
KEGGimmu:16202
UCSCiuc009izb.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94479 mRNA Translation: AAB94646.1
BC003737 mRNA Translation: AAH03737.1
CCDSiCCDS21659.1
RefSeqiNP_001155196.1, NM_001161724.1
NP_034692.2, NM_010562.2
UniGeneiMm.274846

3D structure databases

ProteinModelPortaliO55222
SMRiO55222
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200649, 9 interactors
CORUMiO55222
DIPiDIP-41971N
IntActiO55222, 48 interactors
MINTiO55222
STRINGi10090.ENSMUSP00000033182

PTM databases

iPTMnetiO55222
PhosphoSitePlusiO55222
SwissPalmiO55222

Proteomic databases

EPDiO55222
MaxQBiO55222
PaxDbiO55222
PeptideAtlasiO55222
PRIDEiO55222

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033182; ENSMUSP00000033182; ENSMUSG00000030890
ENSMUST00000163389; ENSMUSP00000130341; ENSMUSG00000030890
GeneIDi16202
KEGGimmu:16202
UCSCiuc009izb.2 mouse

Organism-specific databases

CTDi3611
MGIiMGI:1195267 Ilk

Phylogenomic databases

eggNOGiKOG0195 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00930000150972
HOGENOMiHOG000047828
HOVERGENiHBG002437
InParanoidiO55222
KOiK06272
OMAiNMADAKF
OrthoDBiEOG091G05IK
PhylomeDBiO55222
TreeFamiTF315194

Enzyme and pathway databases

BRENDAi2.7.10.2 3474
ReactomeiR-MMU-446343 Localization of the PINCH-ILK-PARVIN complex to focal adhesions

Miscellaneous databases

PROiPR:O55222
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030890 Expressed in 280 organ(s), highest expression level in uterus
CleanExiMM_ILK
ExpressionAtlasiO55222 baseline and differential
GenevisibleiO55222 MM

Family and domain databases

CDDicd00204 ANK, 1 hit
cd14057 PK_ILK, 1 hit
Gene3Di1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR011009 Kinase-like_dom_sf
IPR035692 PK_ILK
IPR000719 Prot_kinase_dom
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
PfamiView protein in Pfam
PF12796 Ank_2, 2 hits
PF07714 Pkinase_Tyr, 1 hit
SMARTiView protein in SMART
SM00248 ANK, 3 hits
SUPFAMiSSF48403 SSF48403, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 3 hits
PS50011 PROTEIN_KINASE_DOM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiILK_MOUSE
AccessioniPrimary (citable) accession number: O55222
Secondary accession number(s): Q78KK2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 31, 2006
Last modified: November 7, 2018
This is version 183 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again