UniProtKB - O55222 (ILK_MOUSE)
Integrin-linked protein kinase
Ilk
Functioni
Catalytic activityi
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 220 | ATPPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 199 – 207 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- integrin binding Source: MGI
- protein-containing complex binding Source: MGI
- protein domain specific binding Source: MGI
- protein kinase activity Source: MGI
- protein kinase binding Source: MGI
- protein serine/threonine kinase activity Source: MGI
- SH3 domain binding Source: MGI
GO - Biological processi
- branching involved in ureteric bud morphogenesis Source: MGI
- cell aging Source: MGI
- cell cycle arrest Source: MGI
- cell-matrix adhesion Source: GO_Central
- cell projection organization Source: MGI
- establishment or maintenance of epithelial cell apical/basal polarity Source: MGI
- fibroblast migration Source: MGI
- integrin-mediated signaling pathway Source: MGI
- myelin assembly Source: MGI
- myelination in peripheral nervous system Source: MGI
- negative regulation of apoptotic process Source: MGI
- negative regulation of cardiac muscle cell apoptotic process Source: MGI
- negative regulation of neural precursor cell proliferation Source: MGI
- negative regulation of neuron apoptotic process Source: MGI
- negative regulation of protein kinase activity Source: MGI
- negative regulation of smooth muscle cell migration Source: MGI
- negative regulation of smooth muscle cell proliferation Source: MGI
- nerve development Source: MGI
- neuron projection morphogenesis Source: MGI
- outflow tract morphogenesis Source: CACAO
- peptidyl-serine phosphorylation Source: MGI
- positive regulation of axon extension Source: MGI
- positive regulation of axonogenesis Source: MGI
- positive regulation of BMP signaling pathway Source: BHF-UCL
- positive regulation of canonical Wnt signaling pathway Source: MGI
- positive regulation of cell-matrix adhesion Source: MGI
- positive regulation of cell migration Source: MGI
- positive regulation of cell population proliferation Source: MGI
- positive regulation of dendrite morphogenesis Source: MGI
- positive regulation of MAPK cascade Source: MGI
- positive regulation of MAP kinase activity Source: MGI
- positive regulation of myoblast differentiation Source: MGI
- positive regulation of NIK/NF-kappaB signaling Source: MGI
- positive regulation of osteoblast differentiation Source: BHF-UCL
- positive regulation of phosphorylation Source: MGI
- positive regulation of protein kinase B signaling Source: MGI
- positive regulation of protein phosphorylation Source: MGI
- positive regulation of signal transduction Source: MGI
- positive regulation of transcription, DNA-templated Source: MGI
- protein kinase B signaling Source: MGI
- protein phosphorylation Source: MGI
- regulation of actin cytoskeleton organization Source: MGI
- regulation of cell growth Source: MGI
- Schwann cell development Source: MGI
- substrate adhesion-dependent cell spreading Source: MGI
- supramolecular fiber organization Source: MGI
- tumor necrosis factor-mediated signaling pathway Source: MGI
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.10.2, 3474 |
Reactomei | R-MMU-446343, Localization of the PINCH-ILK-PARVIN complex to focal adhesions R-MMU-446353, Cell-extracellular matrix interactions |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Ilk |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1195267, Ilk |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Other locations
- focal adhesion 2 Publications
- sarcomere By similarity
- lamellipodium 1 Publication
Cytoskeleton
- stress fiber Source: Ensembl
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- axon Source: MGI
- cell junction Source: MGI
- cell-cell junction Source: MGI
- costamere Source: MGI
- cytoplasm Source: MGI
- dendritic shaft Source: MGI
- focal adhesion Source: MGI
- lamellipodium Source: UniProtKB
- neuronal cell body Source: MGI
- protein-containing complex Source: MGI
- sarcomere Source: UniProtKB-SubCell
- terminal bouton Source: MGI
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086022 | 1 – 452 | Integrin-linked protein kinaseAdd BLAST | 452 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 186 | PhosphoserineBy similarity | 1 | |
Modified residuei | 426 | N6-acetyllysineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | O55222 |
jPOSTi | O55222 |
MaxQBi | O55222 |
PaxDbi | O55222 |
PeptideAtlasi | O55222 |
PRIDEi | O55222 |
PTM databases
iPTMneti | O55222 |
PhosphoSitePlusi | O55222 |
SwissPalmi | O55222 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000030890, Expressed in uterus and 295 other tissues |
Genevisiblei | O55222, MM |
Interactioni
Subunit structurei
Interacts with FERMT2 (PubMed:18483218).
Interacts with the cytoplasmic domain of ITGB1 (By similarity). Could also interact with integrin ITGB2, ITGB3 and/or ITGB5 (By similarity).
Interacts (via ANK repeats) with LIMS1 and LIMS2 (By similarity).
Interacts with PARVA and PARVB; these compete for the same binding site (By similarity).
Interacts probably also with TGFB1I1 (PubMed:16737959).
Interacts (via ANK repeats) with EPHA1 (via SAM domain); stimulated by EFNA1 but independent of the kinase activity of EPHA1 (By similarity).
Interacts with LIMD2; leading to activate the protein kinase activity (By similarity).
By similarity2 PublicationsBinary interactionsi
Hide detailsO55222
GO - Molecular functioni
- integrin binding Source: MGI
- protein domain specific binding Source: MGI
- protein kinase binding Source: MGI
- SH3 domain binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 200649, 17 interactors |
CORUMi | O55222 |
DIPi | DIP-41971N |
IntActi | O55222, 49 interactors |
MINTi | O55222 |
STRINGi | 10090.ENSMUSP00000033182 |
Miscellaneous databases
RNActi | O55222, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 2 – 30 | ANK 1Add BLAST | 29 | |
Repeati | 31 – 63 | ANK 2Add BLAST | 33 | |
Repeati | 64 – 96 | ANK 3Add BLAST | 33 | |
Repeati | 97 – 129 | ANK 4Add BLAST | 33 | |
Repeati | 130 – 174 | ANK 5Add BLAST | 45 | |
Domaini | 193 – 446 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 254 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 33 – 139 | Interaction with LIMS1By similarityAdd BLAST | 107 | |
Regioni | 180 – 212 | PH-like; mediates interaction with TGFB1I11 PublicationAdd BLAST | 33 |
Domaini
Sequence similaritiesi
Keywords - Domaini
ANK repeat, RepeatPhylogenomic databases
eggNOGi | KOG0195, Eukaryota |
GeneTreei | ENSGT00940000155956 |
HOGENOMi | CLU_000288_7_5_1 |
InParanoidi | O55222 |
OMAi | IVGKLIQ |
OrthoDBi | 740146at2759 |
PhylomeDBi | O55222 |
TreeFami | TF315194 |
Family and domain databases
CDDi | cd14057, PK_ILK, 1 hit |
Gene3Di | 1.25.40.20, 1 hit |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR020683, Ankyrin_rpt-contain_dom IPR036770, Ankyrin_rpt-contain_sf IPR011009, Kinase-like_dom_sf IPR035692, PK_ILK IPR000719, Prot_kinase_dom IPR001245, Ser-Thr/Tyr_kinase_cat_dom |
Pfami | View protein in Pfam PF12796, Ank_2, 1 hit PF07714, PK_Tyr_Ser-Thr, 1 hit |
SMARTi | View protein in SMART SM00248, ANK, 3 hits |
SUPFAMi | SSF48403, SSF48403, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 3 hits PS50011, PROTEIN_KINASE_DOM, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW ACREGRSAVV
60 70 80 90 100
EMLIMRGARI NVMNRGDDTP LHLAASHGHR DIVQKLLQYK ADINAVNEHG
110 120 130 140 150
NVPLHYACFW GQDQVAEDLV ANGALVSICN KYGEMPVDKA KAPLRELLRE
160 170 180 190 200
RAEKMGQNLN RIPYKDTFWK GTTRTRPRNG TLNKHSGIDF KQLNFLAKLN
210 220 230 240 250
ENHSGELWKG RWQGNDIVVK VLKVRDWSTR KSRDFNEECP RLRIFSHPNV
260 270 280 290 300
LPVLGACQAP PAPHPTLITH WMPYGSLYNV LHEGTNFVVD QSQAVKFALD
310 320 330 340 350
MARGMAFLHT LEPLIPRHAL NSRSVMIDED MTARISMADV KFSFQCPGRM
360 370 380 390 400
YAPAWVAPEA LQKKPEDTNR RSADMWSFAV LLWELVTREV PFADLSNMEI
410 420 430 440 450
GMKVALEGLR PTIPPGISPH VCKLMKICMN EDPAKRPKFD MIVPILEKMQ
DK
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3YZA5 | D3YZA5_MOUSE | Integrin-linked protein kinase | Ilk | 295 | Annotation score: | ||
A0A1B0GR42 | A0A1B0GR42_MOUSE | Integrin-linked protein kinase | Ilk | 95 | Annotation score: | ||
A0A1B0GRF6 | A0A1B0GRF6_MOUSE | Integrin-linked protein kinase | Ilk | 233 | Annotation score: | ||
F6Q5Z1 | F6Q5Z1_MOUSE | Integrin-linked protein kinase | Ilk | 17 | Annotation score: | ||
A0A1B0GRW6 | A0A1B0GRW6_MOUSE | Integrin-linked protein kinase | Ilk | 54 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 93 | I → T in AAB94646 (PubMed:9366252).Curated | 1 | |
Sequence conflicti | 413 | I → V in AAB94646 (PubMed:9366252).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U94479 mRNA Translation: AAB94646.1 BC003737 mRNA Translation: AAH03737.1 |
CCDSi | CCDS21659.1 |
RefSeqi | NP_001155196.1, NM_001161724.1 NP_034692.2, NM_010562.2 |
Genome annotation databases
Ensembli | ENSMUST00000033182; ENSMUSP00000033182; ENSMUSG00000030890 ENSMUST00000163389; ENSMUSP00000130341; ENSMUSG00000030890 |
GeneIDi | 16202 |
KEGGi | mmu:16202 |
UCSCi | uc009izb.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U94479 mRNA Translation: AAB94646.1 BC003737 mRNA Translation: AAH03737.1 |
CCDSi | CCDS21659.1 |
RefSeqi | NP_001155196.1, NM_001161724.1 NP_034692.2, NM_010562.2 |
3D structure databases
BMRBi | O55222 |
SMRi | O55222 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 200649, 17 interactors |
CORUMi | O55222 |
DIPi | DIP-41971N |
IntActi | O55222, 49 interactors |
MINTi | O55222 |
STRINGi | 10090.ENSMUSP00000033182 |
PTM databases
iPTMneti | O55222 |
PhosphoSitePlusi | O55222 |
SwissPalmi | O55222 |
Proteomic databases
EPDi | O55222 |
jPOSTi | O55222 |
MaxQBi | O55222 |
PaxDbi | O55222 |
PeptideAtlasi | O55222 |
PRIDEi | O55222 |
Protocols and materials databases
Antibodypediai | 23909, 770 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000033182; ENSMUSP00000033182; ENSMUSG00000030890 ENSMUST00000163389; ENSMUSP00000130341; ENSMUSG00000030890 |
GeneIDi | 16202 |
KEGGi | mmu:16202 |
UCSCi | uc009izb.2, mouse |
Organism-specific databases
CTDi | 3611 |
MGIi | MGI:1195267, Ilk |
Phylogenomic databases
eggNOGi | KOG0195, Eukaryota |
GeneTreei | ENSGT00940000155956 |
HOGENOMi | CLU_000288_7_5_1 |
InParanoidi | O55222 |
OMAi | IVGKLIQ |
OrthoDBi | 740146at2759 |
PhylomeDBi | O55222 |
TreeFami | TF315194 |
Enzyme and pathway databases
BRENDAi | 2.7.10.2, 3474 |
Reactomei | R-MMU-446343, Localization of the PINCH-ILK-PARVIN complex to focal adhesions R-MMU-446353, Cell-extracellular matrix interactions |
Miscellaneous databases
BioGRID-ORCSi | 16202, 3 hits in 19 CRISPR screens |
ChiTaRSi | Ilk, mouse |
PROi | PR:O55222 |
RNActi | O55222, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030890, Expressed in uterus and 295 other tissues |
Genevisiblei | O55222, MM |
Family and domain databases
CDDi | cd14057, PK_ILK, 1 hit |
Gene3Di | 1.25.40.20, 1 hit |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR020683, Ankyrin_rpt-contain_dom IPR036770, Ankyrin_rpt-contain_sf IPR011009, Kinase-like_dom_sf IPR035692, PK_ILK IPR000719, Prot_kinase_dom IPR001245, Ser-Thr/Tyr_kinase_cat_dom |
Pfami | View protein in Pfam PF12796, Ank_2, 1 hit PF07714, PK_Tyr_Ser-Thr, 1 hit |
SMARTi | View protein in SMART SM00248, ANK, 3 hits |
SUPFAMi | SSF48403, SSF48403, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 3 hits PS50011, PROTEIN_KINASE_DOM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ILK_MOUSE | |
Accessioni | O55222Primary (citable) accession number: O55222 Secondary accession number(s): Q78KK2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | October 31, 2006 | |
Last modified: | December 2, 2020 | |
This is version 198 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human and mouse protein kinases
Human and mouse protein kinases: classification and index