UniProtKB - O55201 (SPT5H_MOUSE)
Protein
Transcription elongation factor SPT5
Gene
Supt5h
Organism
Mus musculus (Mouse)
Status
Functioni
Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites (By similarity).By similarity
GO - Molecular functioni
- chromatin binding Source: MGI
- enzyme binding Source: MGI
- mRNA binding Source: GO_Central
- protein heterodimerization activity Source: MGI
GO - Biological processi
- negative regulation of DNA-templated transcription, elongation Source: MGI
- negative regulation of mRNA polyadenylation Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of DNA-templated transcription, elongation Source: MGI
- positive regulation of macroautophagy Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- transcription elongation from RNA polymerase II promoter Source: MGI
Keywordsi
Molecular function | Activator, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-112382 Formation of RNA Pol II elongation complex R-MMU-113418 Formation of the Early Elongation Complex R-MMU-674695 RNA Polymerase II Pre-transcription Events R-MMU-6796648 TP53 Regulates Transcription of DNA Repair Genes R-MMU-6807505 RNA polymerase II transcribes snRNA genes R-MMU-72086 mRNA Capping R-MMU-75955 RNA Polymerase II Transcription Elongation R-MMU-77075 RNA Pol II CTD phosphorylation and interaction with CE |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription elongation factor SPT5Alternative name(s): DRB sensitivity-inducing factor large subunit Short name: DSIF large subunit |
Gene namesi | Name:Supt5h Synonyms:Supt5 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1202400 Supt5 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- DSIF complex Source: UniProtKB
- nucleoplasm Source: MGI
- nucleus Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000208469 | 1 – 1082 | Transcription elongation factor SPT5Add BLAST | 1082 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 32 | PhosphoserineCombined sources | 1 | |
Modified residuei | 36 | PhosphoserineCombined sources | 1 | |
Cross-linki | 141 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 661 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 664 | PhosphoserineCombined sources | 1 | |
Modified residuei | 684 | PhosphoserineBy similarity | 1 | |
Modified residuei | 690 | Asymmetric dimethylarginine; alternateBy similarity | 1 | |
Modified residuei | 690 | Omega-N-methylarginine; alternateCombined sources | 1 | |
Modified residuei | 690 | Omega-N-methylated arginine; alternateBy similarity | 1 | |
Modified residuei | 692 | Asymmetric dimethylarginine; alternateBy similarity | 1 | |
Modified residuei | 692 | Omega-N-methylarginine; alternateBy similarity | 1 | |
Modified residuei | 692 | Omega-N-methylated arginine; alternateBy similarity | 1 | |
Modified residuei | 692 | Symmetric dimethylarginine; alternateBy similarity | 1 | |
Modified residuei | 712 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 769 | Phosphothreonine; by CDK9By similarity | 1 | |
Modified residuei | 778 | Phosphothreonine; by CDK9By similarity | 1 | |
Modified residuei | 783 | PhosphoserineBy similarity | 1 | |
Modified residuei | 785 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 798 | PhosphoserineBy similarity | 1 | |
Modified residuei | 800 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1028 | PhosphothreonineBy similarity | 1 | |
Cross-linki | 1031 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Post-translational modificationi
Methylated by PRMT1/HRMT1L2 and PRMT5/SKB1. Methylation negatively regulates interaction with P-TEFb and RNA polymerase II (By similarity).By similarity
Phosphorylated by CDK7 and CDK9. Phosphorylation by P-TEFb alleviates transcriptional pausing. Phosphorylation may also stimulate interaction with PIN1. Bulk phosphorylation occurs predominantly in mitosis (By similarity).By similarity
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | O55201 |
jPOSTi | O55201 |
MaxQBi | O55201 |
PaxDbi | O55201 |
PeptideAtlasi | O55201 |
PRIDEi | O55201 |
PTM databases
iPTMneti | O55201 |
PhosphoSitePlusi | O55201 |
SwissPalmi | O55201 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000003435 Expressed in 313 organ(s), highest expression level in placenta |
ExpressionAtlasi | O55201 baseline and differential |
Genevisiblei | O55201 MM |
Interactioni
Subunit structurei
Interacts with SUPT4H1 to form DSIF. DSIF interacts with the positive transcription elongation factor b complex (P-TEFb complex), which is composed of CDK9 and cyclin-T (CCNT1 or CCNT2). DSIF interacts with RNA polymerase II, and this interaction is reduced by phosphorylation of the C-terminal domain (CTD) of POLR2A by P-TEFb. DSIF also interacts with the NELF complex, which is composed of NELFA, NELFB, NELFD and NELFE, and this interaction occurs following prior binding of DSIF to RNA polymerase II. Also interacts with PRMT1/HRMT1L2, HTATSF1/TATSF1, RNGTT/CAP1A, PRMT5/SKB1, SUPT6H, and can interact with PIN1. Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin (By similarity).By similarity
GO - Molecular functioni
- enzyme binding Source: MGI
- protein heterodimerization activity Source: MGI
Protein-protein interaction databases
BioGridi | 203575, 16 interactors |
IntActi | O55201, 2 interactors |
MINTi | O55201 |
STRINGi | 10090.ENSMUSP00000003527 |
Structurei
3D structure databases
ProteinModelPortali | O55201 |
SMRi | O55201 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 271 – 304 | KOW 1Add BLAST | 34 | |
Domaini | 418 – 449 | KOW 2Add BLAST | 32 | |
Domaini | 470 – 501 | KOW 3Add BLAST | 32 | |
Domaini | 592 – 625 | KOW 4Add BLAST | 34 | |
Domaini | 698 – 731 | KOW 5Add BLAST | 34 | |
Repeati | 748 – 753 | CTR1-1; approximate | 6 | |
Repeati | 754 – 759 | CTR1-2 | 6 | |
Repeati | 760 – 765 | CTR1-3 | 6 | |
Repeati | 766 – 772 | CTR1-4 | 7 | |
Repeati | 775 – 781 | CTR1-5 | 7 | |
Repeati | 782 – 788 | CTR1-6 | 7 | |
Repeati | 790 – 796 | CTR1-7 | 7 | |
Repeati | 797 – 803 | CTR1-8 | 7 | |
Repeati | 805 – 811 | CTR1-9 | 7 | |
Repeati | 838 – 845 | CTR2-1 | 8 | |
Repeati | 848 – 856 | CTR2-2; approximate | 9 | |
Repeati | 857 – 863 | CTR2-3; approximate | 7 | |
Repeati | 875 – 879 | CTR2-4; half-length | 5 | |
Repeati | 890 – 896 | CTR2-5; approximate | 7 | |
Repeati | 898 – 905 | CTR2-6 | 8 | |
Repeati | 910 – 915 | CTR2-7; approximate | 6 | |
Repeati | 918 – 924 | CTR2-8 | 7 | |
Repeati | 926 – 933 | CTR2-9 | 8 | |
Repeati | 937 – 944 | CTR2-10 | 8 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 174 – 268 | Interaction with SUPT4H1 and SUPT4H2By similarityAdd BLAST | 95 | |
Regioni | 311 – 418 | Interaction with RNA polymerase IIBy similarityAdd BLAST | 108 | |
Regioni | 748 – 811 | 9 X 7 AA approximate tandem repeats of G-S-[QR]-T-P-X-[YQ], motif CTR1Add BLAST | 64 | |
Regioni | 838 – 944 | 10 X 8 AA approximate tandem repeats of P-[TS]-P-S-P-[QA]-[SG]-Y, motif CTR2Add BLAST | 107 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 11 – 104 | Glu-richAdd BLAST | 94 |
Sequence similaritiesi
Belongs to the SPT5 family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1999 Eukaryota COG0250 LUCA COG5164 LUCA |
GeneTreei | ENSGT00440000037640 |
HOVERGENi | HBG079775 |
InParanoidi | O55201 |
KOi | K15172 |
OMAi | GYMNTPS |
OrthoDBi | 828863at2759 |
PhylomeDBi | O55201 |
TreeFami | TF105730 |
Family and domain databases
CDDi | cd09888 NGN_Euk, 1 hit |
Gene3Di | 2.30.30.30, 3 hits 3.30.70.940, 1 hit |
InterProi | View protein in InterPro IPR005824 KOW IPR005100 NGN-domain IPR006645 NGN_dom IPR036735 NGN_dom_sf IPR039385 NGN_Euk IPR014722 Rib_L2_dom2 IPR039659 SPT5 IPR024945 Spt5_C_dom IPR022581 Spt5_N IPR017071 TF_Spt5_eukaryote IPR008991 Translation_prot_SH3-like_sf |
PANTHERi | PTHR11125 PTHR11125, 1 hit |
Pfami | View protein in Pfam PF00467 KOW, 1 hit PF03439 Spt5-NGN, 1 hit PF11942 Spt5_N, 1 hit |
PIRSFi | PIRSF036945 Spt5, 1 hit |
SMARTi | View protein in SMART SM01104 CTD, 1 hit SM00739 KOW, 6 hits SM00738 NGN, 1 hit |
SUPFAMi | SSF50104 SSF50104, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: O55201-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MSDSEDSNFS EEEDSERSSE AEEAEVEEDQ RSAAGSEKEE EPEEEEEEEE
60 70 80 90 100
EYDEEEEEED DDRPPKKPRH GGFILDEADV DDEYEDEDQW EDGAEDILEK
110 120 130 140 150
EEIEASNIDN VVLDEDRSGA RRLQNLWRDQ REEELGEYYM KKYAKSSVGE
160 170 180 190 200
TVYGGSDELS DDITQQQLLP GVKDPNLWTV KCKIGEERAT AISLMRKFIA
210 220 230 240 250
YQFTDTPLQI KSVVAPEHVK GYIYVEAYKQ THVKQAIEGV GNLRLGYWNQ
260 270 280 290 300
QMVPIKEMTD VLKVVKEVAN LKPKSWVRLK RGIYKDDIAQ VDYVEPSQNT
310 320 330 340 350
ISLKMIPRID YDRIKARMSL KDWFAKRKKF KRPPQRLFDA EKIRSLGGDV
360 370 380 390 400
ASDGDFLIFE GNRYSRKGFL FKSFAMSAVI TEGVKPTLSE LEKFEDQPEG
410 420 430 440 450
IDLEVVTEST GKEREHNFQP GDNVEVCEGE LINLQGKVLS VDGNKITIMP
460 470 480 490 500
KHEDLKDMLE FPAQELRKYF KMGDHVKVIA GRFEGDTGLI VRVEENFVIL
510 520 530 540 550
FSDLTMHELK VLPRDLQLCS ETASGVDVGG QHEWGELVQL DPRTVGVIVR
560 570 580 590 600
LERETFQVLN MHGKVVTVRH QAVTQKKDNR FAVALDSDQN NIHVKDIVKV
610 620 630 640 650
IDGPHSGREG EIRHLYRSFA FLHCKKLVEN GGMFVCKARH LVLAGGSKPR
660 670 680 690 700
DVTNLTVGGF TPMSPRISSP MHPSAEGQHG GFGSPGGMSR GRGRRDNELI
710 720 730 740 750
GQTVRISQGP YKGYIGVVKD ATESTARVEL HSTCQTISVD RQRLTTVDSQ
760 770 780 790 800
RPGGMTSTYG RTPMYGSQTP MYGSGSRTPM YGSQTPLQDG SRTPHYGSQT
810 820 830 840 850
PLHDGSRTPA QSGAWDPNNP NTPSRAEEEY EYAFDDEPTP SPQAYGGTPN
860 870 880 890 900
PQTPGYPDPS SPQVNPQYNP QTPGTPAMYN TDQFSPYAAP SPQGSYQPSP
910 920 930 940 950
SPQSYHQVAP SPAGYQNTHS PASYHPTPSP MAYQASPSPS PVGYSPMTPG
960 970 980 990 1000
APSPGGYNPH TPGSGIEQNS SDWVTTDIQV KVRDTYLDTQ IVGQTGVIRS
1010 1020 1030 1040 1050
VTGGMCSVYL KDSEKVVSIS SEHLEPITPT KNNKVKVILG EDREATGVLL
1060 1070 1080
SIDGEDGIIR MDLEDQQIKI LNLRFLGKLL EA
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A140LJ34 | A0A140LJ34_MOUSE | Suppressor of Ty 5 | Supt5 | 149 | Annotation score: | ||
A0A140LIU5 | A0A140LIU5_MOUSE | Suppressor of Ty 5 | Supt5 | 52 | Annotation score: |
Sequence cautioni
The sequence AAH57449 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 30 | Q → R in BAE26244 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 51 | E → EE in BAE26244 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 111 | V → L in BAE27278 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 229 | K → E in BAE27184 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 265 | V → E in BAE26864 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 416 | H → N in AAH58598 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 775 | G → D in BAE26864 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 1063 | L → I in BAE26864 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_016283 | 1 – 194 | Missing in isoform 2. 1 PublicationAdd BLAST | 194 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U88539 mRNA Translation: AAC40052.1 AK145117 mRNA Translation: BAE26244.1 AK146055 mRNA Translation: BAE26864.1 AK146453 mRNA Translation: BAE27184.1 AK146583 mRNA Translation: BAE27278.1 AK146650 mRNA Translation: BAE27330.1 BC007132 mRNA Translation: AAH07132.1 BC057449 mRNA Translation: AAH57449.1 Different initiation. BC058598 mRNA Translation: AAH58598.1 BC059849 mRNA Translation: AAH59849.1 |
CCDSi | CCDS39857.1 [O55201-1] |
PIRi | T42204 |
RefSeqi | NP_038704.1, NM_013676.1 [O55201-1] |
UniGenei | Mm.285906 |
Genome annotation databases
Ensembli | ENSMUST00000003527; ENSMUSP00000003527; ENSMUSG00000003435 [O55201-1] ENSMUST00000209141; ENSMUSP00000147164; ENSMUSG00000003435 [O55201-1] |
GeneIDi | 20924 |
KEGGi | mmu:20924 |
UCSCi | uc009fyh.1 mouse [O55201-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U88539 mRNA Translation: AAC40052.1 AK145117 mRNA Translation: BAE26244.1 AK146055 mRNA Translation: BAE26864.1 AK146453 mRNA Translation: BAE27184.1 AK146583 mRNA Translation: BAE27278.1 AK146650 mRNA Translation: BAE27330.1 BC007132 mRNA Translation: AAH07132.1 BC057449 mRNA Translation: AAH57449.1 Different initiation. BC058598 mRNA Translation: AAH58598.1 BC059849 mRNA Translation: AAH59849.1 |
CCDSi | CCDS39857.1 [O55201-1] |
PIRi | T42204 |
RefSeqi | NP_038704.1, NM_013676.1 [O55201-1] |
UniGenei | Mm.285906 |
3D structure databases
ProteinModelPortali | O55201 |
SMRi | O55201 |
ModBasei | Search... |
MobiDBi | Search... |
Protein-protein interaction databases
BioGridi | 203575, 16 interactors |
IntActi | O55201, 2 interactors |
MINTi | O55201 |
STRINGi | 10090.ENSMUSP00000003527 |
PTM databases
iPTMneti | O55201 |
PhosphoSitePlusi | O55201 |
SwissPalmi | O55201 |
Proteomic databases
EPDi | O55201 |
jPOSTi | O55201 |
MaxQBi | O55201 |
PaxDbi | O55201 |
PeptideAtlasi | O55201 |
PRIDEi | O55201 |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Genome annotation databases
Ensembli | ENSMUST00000003527; ENSMUSP00000003527; ENSMUSG00000003435 [O55201-1] ENSMUST00000209141; ENSMUSP00000147164; ENSMUSG00000003435 [O55201-1] |
GeneIDi | 20924 |
KEGGi | mmu:20924 |
UCSCi | uc009fyh.1 mouse [O55201-1] |
Organism-specific databases
CTDi | 20924 |
MGIi | MGI:1202400 Supt5 |
Phylogenomic databases
eggNOGi | KOG1999 Eukaryota COG0250 LUCA COG5164 LUCA |
GeneTreei | ENSGT00440000037640 |
HOVERGENi | HBG079775 |
InParanoidi | O55201 |
KOi | K15172 |
OMAi | GYMNTPS |
OrthoDBi | 828863at2759 |
PhylomeDBi | O55201 |
TreeFami | TF105730 |
Enzyme and pathway databases
Reactomei | R-MMU-112382 Formation of RNA Pol II elongation complex R-MMU-113418 Formation of the Early Elongation Complex R-MMU-674695 RNA Polymerase II Pre-transcription Events R-MMU-6796648 TP53 Regulates Transcription of DNA Repair Genes R-MMU-6807505 RNA polymerase II transcribes snRNA genes R-MMU-72086 mRNA Capping R-MMU-75955 RNA Polymerase II Transcription Elongation R-MMU-77075 RNA Pol II CTD phosphorylation and interaction with CE |
Miscellaneous databases
ChiTaRSi | Supt5 mouse |
PROi | PR:O55201 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000003435 Expressed in 313 organ(s), highest expression level in placenta |
ExpressionAtlasi | O55201 baseline and differential |
Genevisiblei | O55201 MM |
Family and domain databases
CDDi | cd09888 NGN_Euk, 1 hit |
Gene3Di | 2.30.30.30, 3 hits 3.30.70.940, 1 hit |
InterProi | View protein in InterPro IPR005824 KOW IPR005100 NGN-domain IPR006645 NGN_dom IPR036735 NGN_dom_sf IPR039385 NGN_Euk IPR014722 Rib_L2_dom2 IPR039659 SPT5 IPR024945 Spt5_C_dom IPR022581 Spt5_N IPR017071 TF_Spt5_eukaryote IPR008991 Translation_prot_SH3-like_sf |
PANTHERi | PTHR11125 PTHR11125, 1 hit |
Pfami | View protein in Pfam PF00467 KOW, 1 hit PF03439 Spt5-NGN, 1 hit PF11942 Spt5_N, 1 hit |
PIRSFi | PIRSF036945 Spt5, 1 hit |
SMARTi | View protein in SMART SM01104 CTD, 1 hit SM00739 KOW, 6 hits SM00738 NGN, 1 hit |
SUPFAMi | SSF50104 SSF50104, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | SPT5H_MOUSE | |
Accessioni | O55201Primary (citable) accession number: O55201 Secondary accession number(s): Q3UJ77 Q6PFR4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 22, 2005 |
Last sequence update: | June 1, 1998 | |
Last modified: | February 13, 2019 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot