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UniProtKB - O55201 (SPT5H_MOUSE)

Entry version 169 (07 Oct 2020)
Sequence version 1 (01 Jun 1998)
Previous versions | rss
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Protein

Transcription elongation factor SPT5

Gene

Supt5h

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites (By similarity).By similarity

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionActivator, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-MMU-112382, Formation of RNA Pol II elongation complex
R-MMU-113418, Formation of the Early Elongation Complex
R-MMU-674695, RNA Polymerase II Pre-transcription Events
R-MMU-6796648, TP53 Regulates Transcription of DNA Repair Genes
R-MMU-6807505, RNA polymerase II transcribes snRNA genes
R-MMU-72086, mRNA Capping
R-MMU-75955, RNA Polymerase II Transcription Elongation
R-MMU-77075, RNA Pol II CTD phosphorylation and interaction with CE

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Transcription elongation factor SPT5
Alternative name(s):
DRB sensitivity-inducing factor large subunit
Short name:
DSIF large subunit
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Supt5h
Synonyms:Supt5
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 7

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...MGIi		MGI:1202400, Supt5

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002084691 – 1082Transcription elongation factor SPT5Add BLAST1082

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei32PhosphoserineCombined sources1
Modified residuei36PhosphoserineCombined sources1
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section describes <strong>covalent linkages</strong> of various types formed <strong>between two proteins (interchain cross-links)</strong> or <strong>between two parts of the same protein (intrachain cross-links)</strong>, except the disulfide bonds that are annotated in the <a href="http://www.uniprot.org/manual/disulfid">'Disulfide bond'</a> subsection.<p><a href='/help/crosslnk' target='_top'>More...</a></p>Cross-linki141Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei661PhosphothreonineCombined sources1
Modified residuei664PhosphoserineCombined sources1
Modified residuei684PhosphoserineBy similarity1
Modified residuei690Asymmetric dimethylarginine; alternateBy similarity1
Modified residuei690Omega-N-methylarginine; alternateCombined sources1
Modified residuei692Asymmetric dimethylarginine; alternateBy similarity1
Modified residuei692Omega-N-methylarginine; alternateBy similarity1
Modified residuei692Symmetric dimethylarginine; alternateBy similarity1
Modified residuei712N6-acetyllysineCombined sources1
Modified residuei769Phosphothreonine; by CDK9By similarity1
Modified residuei778Phosphothreonine; by CDK9By similarity1
Modified residuei783PhosphoserineBy similarity1
Modified residuei785PhosphothreonineBy similarity1
Modified residuei798PhosphoserineBy similarity1
Modified residuei800PhosphothreonineBy similarity1
Modified residuei1028PhosphothreonineBy similarity1
Cross-linki1031Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Methylated by PRMT1/HRMT1L2 and PRMT5/SKB1. Methylation negatively regulates interaction with P-TEFb and RNA polymerase II (By similarity).By similarity
Phosphorylated by CDK7 and CDK9. Phosphorylation by P-TEFb alleviates transcriptional pausing. Phosphorylation may also stimulate interaction with PIN1. Bulk phosphorylation occurs predominantly in mitosis (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		O55201

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		O55201

MaxQB - The MaxQuant DataBase

More...MaxQBi		O55201

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		O55201

PeptideAtlas

More...PeptideAtlasi		O55201

PRoteomics IDEntifications database

More...PRIDEi		O55201

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		O55201

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		O55201

SwissPalm database of S-palmitoylation events

More...SwissPalmi		O55201

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSMUSG00000003435, Expressed in placenta and 328 other tissues

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		O55201, MM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with SUPT4H1 to form DSIF. DSIF interacts with the positive transcription elongation factor b complex (P-TEFb complex), which is composed of CDK9 and cyclin-T (CCNT1 or CCNT2). DSIF interacts with RNA polymerase II, and this interaction is reduced by phosphorylation of the C-terminal domain (CTD) of POLR2A by P-TEFb. DSIF also interacts with the NELF complex, which is composed of NELFA, NELFB, NELFD and NELFE, and this interaction occurs following prior binding of DSIF to RNA polymerase II.

Also interacts with PRMT1/HRMT1L2, HTATSF1/TATSF1, RNGTT/CAP1A, PRMT5/SKB1, SUPT6H, and can interact with PIN1.

Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin (By similarity).

Interacts with MCM3AP (By similarity).

By similarity

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		203575, 30 interactors

Protein interaction database and analysis system

More...IntActi		O55201, 2 interactors

Molecular INTeraction database

More...MINTi		O55201

STRING: functional protein association networks

More...STRINGi		10090.ENSMUSP00000003527

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		O55201, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		O55201

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini271 – 304KOW 1Add BLAST34
Domaini418 – 449KOW 2Add BLAST32
Domaini470 – 501KOW 3Add BLAST32
Domaini592 – 625KOW 4Add BLAST34
Domaini698 – 731KOW 5Add BLAST34
<p>This subsection of the 'Family and Domains' section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.<p><a href='/help/repeat' target='_top'>More...</a></p>Repeati748 – 753CTR1-1; approximate6
Repeati754 – 759CTR1-26
Repeati760 – 765CTR1-36
Repeati766 – 772CTR1-47
Repeati775 – 781CTR1-57
Repeati782 – 788CTR1-67
Repeati790 – 796CTR1-77
Repeati797 – 803CTR1-87
Repeati805 – 811CTR1-97
Repeati838 – 845CTR2-18
Repeati848 – 856CTR2-2; approximate9
Repeati857 – 863CTR2-3; approximate7
Repeati875 – 879CTR2-4; half-length5
Repeati890 – 896CTR2-5; approximate7
Repeati898 – 905CTR2-68
Repeati910 – 915CTR2-7; approximate6
Repeati918 – 924CTR2-87
Repeati926 – 933CTR2-98
Repeati937 – 944CTR2-108

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni174 – 268Interaction with SUPT4H1 and SUPT4H2By similarityAdd BLAST95
Regioni311 – 418Interaction with RNA polymerase IIBy similarityAdd BLAST108
Regioni748 – 8119 X 7 AA approximate tandem repeats of G-S-[QR]-T-P-X-[YQ], motif CTR1Add BLAST64
Regioni838 – 94410 X 8 AA approximate tandem repeats of P-[TS]-P-S-P-[QA]-[SG]-Y, motif CTR2Add BLAST107

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi11 – 104Glu-richAdd BLAST94

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the SPT5 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG1999, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00440000037640

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_003537_0_0_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		O55201

KEGG Orthology (KO)

More...KOi		K15172

Identification of Orthologs from Complete Genome Data

More...OMAi		GYMNTPS

Database of Orthologous Groups

More...OrthoDBi		828863at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		O55201

TreeFam database of animal gene trees

More...TreeFami		TF105730

Family and domain databases

Conserved Domains Database

More...CDDi		cd06081, KOW_Spt5_1, 1 hit
cd06082, KOW_Spt5_2, 1 hit
cd06083, KOW_Spt5_3, 1 hit
cd06084, KOW_Spt5_4, 1 hit
cd06085, KOW_Spt5_5, 1 hit
cd06086, KOW_Spt5_6, 1 hit
cd09888, NGN_Euk, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		2.30.30.30, 3 hits
3.30.70.940, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR005824, KOW
IPR041973, KOW_Spt5_1
IPR041975, KOW_Spt5_2
IPR041976, KOW_Spt5_3
IPR041977, KOW_Spt5_4
IPR041978, KOW_Spt5_5
IPR041980, KOW_Spt5_6
IPR005100, NGN-domain
IPR006645, NGN_dom
IPR036735, NGN_dom_sf
IPR039385, NGN_Euk
IPR014722, Rib_L2_dom2
IPR039659, SPT5
IPR024945, Spt5_C_dom
IPR022581, Spt5_N
IPR017071, TF_Spt5_eukaryote
IPR008991, Translation_prot_SH3-like_sf

The PANTHER Classification System

More...PANTHERi		PTHR11125, PTHR11125, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00467, KOW, 1 hit
PF03439, Spt5-NGN, 1 hit
PF11942, Spt5_N, 1 hit

PIRSF; a whole-protein classification database

More...PIRSFi		PIRSF036945, Spt5, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM01104, CTD, 1 hit
SM00739, KOW, 6 hits
SM00738, NGN, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF50104, SSF50104, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O55201-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSDSEDSNFS EEEDSERSSE AEEAEVEEDQ RSAAGSEKEE EPEEEEEEEE 
        60         70         80         90        100
EYDEEEEEED DDRPPKKPRH GGFILDEADV DDEYEDEDQW EDGAEDILEK 
       110        120        130        140        150
EEIEASNIDN VVLDEDRSGA RRLQNLWRDQ REEELGEYYM KKYAKSSVGE 
       160        170        180        190        200
TVYGGSDELS DDITQQQLLP GVKDPNLWTV KCKIGEERAT AISLMRKFIA 
       210        220        230        240        250
YQFTDTPLQI KSVVAPEHVK GYIYVEAYKQ THVKQAIEGV GNLRLGYWNQ 
       260        270        280        290        300
QMVPIKEMTD VLKVVKEVAN LKPKSWVRLK RGIYKDDIAQ VDYVEPSQNT 
       310        320        330        340        350
ISLKMIPRID YDRIKARMSL KDWFAKRKKF KRPPQRLFDA EKIRSLGGDV 
       360        370        380        390        400
ASDGDFLIFE GNRYSRKGFL FKSFAMSAVI TEGVKPTLSE LEKFEDQPEG 
       410        420        430        440        450
IDLEVVTEST GKEREHNFQP GDNVEVCEGE LINLQGKVLS VDGNKITIMP 
       460        470        480        490        500
KHEDLKDMLE FPAQELRKYF KMGDHVKVIA GRFEGDTGLI VRVEENFVIL 
       510        520        530        540        550
FSDLTMHELK VLPRDLQLCS ETASGVDVGG QHEWGELVQL DPRTVGVIVR 
       560        570        580        590        600
LERETFQVLN MHGKVVTVRH QAVTQKKDNR FAVALDSDQN NIHVKDIVKV 
       610        620        630        640        650
IDGPHSGREG EIRHLYRSFA FLHCKKLVEN GGMFVCKARH LVLAGGSKPR 
       660        670        680        690        700
DVTNLTVGGF TPMSPRISSP MHPSAEGQHG GFGSPGGMSR GRGRRDNELI 
       710        720        730        740        750
GQTVRISQGP YKGYIGVVKD ATESTARVEL HSTCQTISVD RQRLTTVDSQ 
       760        770        780        790        800
RPGGMTSTYG RTPMYGSQTP MYGSGSRTPM YGSQTPLQDG SRTPHYGSQT 
       810        820        830        840        850
PLHDGSRTPA QSGAWDPNNP NTPSRAEEEY EYAFDDEPTP SPQAYGGTPN 
       860        870        880        890        900
PQTPGYPDPS SPQVNPQYNP QTPGTPAMYN TDQFSPYAAP SPQGSYQPSP 
       910        920        930        940        950
SPQSYHQVAP SPAGYQNTHS PASYHPTPSP MAYQASPSPS PVGYSPMTPG 
       960        970        980        990       1000
APSPGGYNPH TPGSGIEQNS SDWVTTDIQV KVRDTYLDTQ IVGQTGVIRS 
      1010       1020       1030       1040       1050
VTGGMCSVYL KDSEKVVSIS SEHLEPITPT KNNKVKVILG EDREATGVLL 
      1060       1070       1080 
SIDGEDGIIR MDLEDQQIKI LNLRFLGKLL EA
Length:1,082
Mass (Da):120,664
Last modified:June 1, 1998 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i109D0D8D2D71DDE7
GO
Isoform 2 (identifier: O55201-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-194: Missing.

Show »
Length:888
Mass (Da):98,310
Checksum:i30E132908E6A5F03
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A140LIU5A0A140LIU5_MOUSE
Suppressor of Ty 5, DSIF elongation...
Supt5
52Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A140LJ34A0A140LJ34_MOUSE
Suppressor of Ty 5, DSIF elongation...
Supt5
149Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAH57449 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti30Q → R in BAE26244 (PubMed:16141072).Curated1
Sequence conflicti51E → EE in BAE26244 (PubMed:16141072).Curated1
Sequence conflicti111V → L in BAE27278 (PubMed:16141072).Curated1
Sequence conflicti229K → E in BAE27184 (PubMed:16141072).Curated1
Sequence conflicti265V → E in BAE26864 (PubMed:16141072).Curated1
Sequence conflicti416H → N in AAH58598 (PubMed:15489334).Curated1
Sequence conflicti775G → D in BAE26864 (PubMed:16141072).Curated1
Sequence conflicti1063L → I in BAE26864 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0162831 – 194Missing in isoform 2. 1 PublicationAdd BLAST194

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
U88539 mRNA Translation: AAC40052.1
AK145117 mRNA Translation: BAE26244.1
AK146055 mRNA Translation: BAE26864.1
AK146453 mRNA Translation: BAE27184.1
AK146583 mRNA Translation: BAE27278.1
AK146650 mRNA Translation: BAE27330.1
BC007132 mRNA Translation: AAH07132.1
BC057449 mRNA Translation: AAH57449.1 Different initiation.
BC058598 mRNA Translation: AAH58598.1
BC059849 mRNA Translation: AAH59849.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS39857.1 [O55201-1]

Protein sequence database of the Protein Information Resource

More...PIRi		T42204

NCBI Reference Sequences

More...RefSeqi		NP_038704.1, NM_013676.1 [O55201-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENSMUST00000003527; ENSMUSP00000003527; ENSMUSG00000003435 [O55201-1]
ENSMUST00000209141; ENSMUSP00000147164; ENSMUSG00000003435 [O55201-1]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		20924

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		mmu:20924

UCSC genome browser

More...UCSCi		uc009fyh.1, mouse [O55201-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88539 mRNA Translation: AAC40052.1
AK145117 mRNA Translation: BAE26244.1
AK146055 mRNA Translation: BAE26864.1
AK146453 mRNA Translation: BAE27184.1
AK146583 mRNA Translation: BAE27278.1
AK146650 mRNA Translation: BAE27330.1
BC007132 mRNA Translation: AAH07132.1
BC057449 mRNA Translation: AAH57449.1 Different initiation.
BC058598 mRNA Translation: AAH58598.1
BC059849 mRNA Translation: AAH59849.1
CCDSiCCDS39857.1 [O55201-1]
PIRiT42204
RefSeqiNP_038704.1, NM_013676.1 [O55201-1]

3D structure databases

SMRiO55201
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi203575, 30 interactors
IntActiO55201, 2 interactors
MINTiO55201
STRINGi10090.ENSMUSP00000003527

PTM databases

iPTMnetiO55201
PhosphoSitePlusiO55201
SwissPalmiO55201

Proteomic databases

EPDiO55201
jPOSTiO55201
MaxQBiO55201
PaxDbiO55201
PeptideAtlasiO55201
PRIDEiO55201

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		16797, 173 antibodies

Genome annotation databases

EnsembliENSMUST00000003527; ENSMUSP00000003527; ENSMUSG00000003435 [O55201-1]
ENSMUST00000209141; ENSMUSP00000147164; ENSMUSG00000003435 [O55201-1]
GeneIDi20924
KEGGimmu:20924
UCSCiuc009fyh.1, mouse [O55201-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		20924
MGIiMGI:1202400, Supt5

Phylogenomic databases

eggNOGiKOG1999, Eukaryota
GeneTreeiENSGT00440000037640
HOGENOMiCLU_003537_0_0_1
InParanoidiO55201
KOiK15172
OMAiGYMNTPS
OrthoDBi828863at2759
PhylomeDBiO55201
TreeFamiTF105730

Enzyme and pathway databases

ReactomeiR-MMU-112382, Formation of RNA Pol II elongation complex
R-MMU-113418, Formation of the Early Elongation Complex
R-MMU-674695, RNA Polymerase II Pre-transcription Events
R-MMU-6796648, TP53 Regulates Transcription of DNA Repair Genes
R-MMU-6807505, RNA polymerase II transcribes snRNA genes
R-MMU-72086, mRNA Capping
R-MMU-75955, RNA Polymerase II Transcription Elongation
R-MMU-77075, RNA Pol II CTD phosphorylation and interaction with CE

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		20924, 7 hits in 22 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		Supt5, mouse

Protein Ontology

More...PROi		PR:O55201
RNActiO55201, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSMUSG00000003435, Expressed in placenta and 328 other tissues
GenevisibleiO55201, MM

Family and domain databases

CDDicd06081, KOW_Spt5_1, 1 hit
cd06082, KOW_Spt5_2, 1 hit
cd06083, KOW_Spt5_3, 1 hit
cd06084, KOW_Spt5_4, 1 hit
cd06085, KOW_Spt5_5, 1 hit
cd06086, KOW_Spt5_6, 1 hit
cd09888, NGN_Euk, 1 hit
Gene3Di2.30.30.30, 3 hits
3.30.70.940, 1 hit
InterProiView protein in InterPro
IPR005824, KOW
IPR041973, KOW_Spt5_1
IPR041975, KOW_Spt5_2
IPR041976, KOW_Spt5_3
IPR041977, KOW_Spt5_4
IPR041978, KOW_Spt5_5
IPR041980, KOW_Spt5_6
IPR005100, NGN-domain
IPR006645, NGN_dom
IPR036735, NGN_dom_sf
IPR039385, NGN_Euk
IPR014722, Rib_L2_dom2
IPR039659, SPT5
IPR024945, Spt5_C_dom
IPR022581, Spt5_N
IPR017071, TF_Spt5_eukaryote
IPR008991, Translation_prot_SH3-like_sf
PANTHERiPTHR11125, PTHR11125, 1 hit
PfamiView protein in Pfam
PF00467, KOW, 1 hit
PF03439, Spt5-NGN, 1 hit
PF11942, Spt5_N, 1 hit
PIRSFiPIRSF036945, Spt5, 1 hit
SMARTiView protein in SMART
SM01104, CTD, 1 hit
SM00739, KOW, 6 hits
SM00738, NGN, 1 hit
SUPFAMiSSF50104, SSF50104, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSPT5H_MOUSE
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O55201
Secondary accession number(s): Q3UJ77
, Q3UJH1, Q3UKD7, Q3UM54, Q6PB73, Q6PDP0, Q6PFR4
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: June 1, 1998
Last modified: October 7, 2020
This is version 169 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families
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