UniProtKB - O55192 (SC6A2_MOUSE)
Protein
Sodium-dependent noradrenaline transporter
Gene
Slc6a2
Organism
Mus musculus (Mouse)
Status
Functioni
Amine transporter. Terminates the action of noradrenaline by its high affinity sodium-dependent reuptake into presynaptic terminals.1 Publication
Miscellaneous
This protein is the target of psychomotor stimulants such as amphetamines or cocaine.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 71 | Sodium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 73 | Sodium 2; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 74 | Sodium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 78 | Sodium 2By similarity | 1 | |
Metal bindingi | 318 | Sodium 2By similarity | 1 | |
Metal bindingi | 350 | Sodium 2By similarity | 1 | |
Metal bindingi | 415 | Sodium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 418 | Sodium 1By similarity | 1 | |
Metal bindingi | 419 | Sodium 1By similarity | 1 |
GO - Molecular functioni
- actin binding Source: MGI
- alpha-tubulin binding Source: MGI
- beta-tubulin binding Source: MGI
- dopamine:sodium symporter activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- monoamine transmembrane transporter activity Source: ARUK-UCL
- neurotransmitter:sodium symporter activity Source: InterPro
- neurotransmitter transmembrane transporter activity Source: ARUK-UCL
- norepinephrine:sodium symporter activity Source: MGI
GO - Biological processi
- dopamine uptake involved in synaptic transmission Source: GO_Central
- monoamine transport Source: MGI
- neurotransmitter reuptake Source: SynGO
- neurotransmitter transport Source: MGI
- norepinephrine transport Source: MGI
- norepinephrine uptake Source: ARUK-UCL
- response to drug Source: Ensembl
- response to pain Source: MGI
- sodium ion transmembrane transport Source: GO_Central
Keywordsi
Biological process | Neurotransmitter transport, Symport, Transport |
Ligand | Metal-binding, Sodium |
Enzyme and pathway databases
Reactomei | R-MMU-442660, Na+/Cl- dependent neurotransmitter transporters |
Names & Taxonomyi
Protein namesi | Recommended name: Sodium-dependent noradrenaline transporterAlternative name(s): Norepinephrine transporter Short name: NET Solute carrier family 6 member 2 |
Gene namesi | Name:Slc6a2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1270850, Slc6a2 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- neuronal cell body membrane Source: GO_Central
- plasma membrane Source: MGI
- presynaptic membrane Source: GO_Central
Other locations
- cell surface Source: MGI
- membrane raft Source: MGI
- neuron projection Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 62 | CytoplasmicBy similarityAdd BLAST | 62 | |
Transmembranei | 63 – 88 | Helical; Name=1By similarityAdd BLAST | 26 | |
Topological domaini | 89 – 92 | ExtracellularBy similarity | 4 | |
Transmembranei | 93 – 116 | Helical; Name=2By similarityAdd BLAST | 24 | |
Topological domaini | 117 – 135 | CytoplasmicBy similarityAdd BLAST | 19 | |
Transmembranei | 136 – 166 | Helical; Name=3By similarityAdd BLAST | 31 | |
Topological domaini | 167 – 233 | ExtracellularBy similarityAdd BLAST | 67 | |
Transmembranei | 234 – 254 | Helical; Name=4By similarityAdd BLAST | 21 | |
Topological domaini | 255 – 257 | CytoplasmicBy similarity | 3 | |
Transmembranei | 258 – 282 | Helical; Name=5By similarityAdd BLAST | 25 | |
Topological domaini | 283 – 306 | ExtracellularBy similarityAdd BLAST | 24 | |
Transmembranei | 307 – 332 | Helical; Name=6By similarityAdd BLAST | 26 | |
Topological domaini | 333 – 338 | CytoplasmicBy similarity | 6 | |
Transmembranei | 339 – 362 | Helical; Name=7By similarityAdd BLAST | 24 | |
Topological domaini | 363 – 402 | ExtracellularBy similarityAdd BLAST | 40 | |
Transmembranei | 403 – 428 | Helical; Name=8By similarityAdd BLAST | 26 | |
Topological domaini | 429 – 443 | CytoplasmicBy similarityAdd BLAST | 15 | |
Transmembranei | 444 – 464 | Helical; Name=9By similarityAdd BLAST | 21 | |
Topological domaini | 465 | ExtracellularBy similarity | 1 | |
Transmembranei | 466 – 492 | Helical; Name=10By similarityAdd BLAST | 27 | |
Topological domaini | 493 – 522 | CytoplasmicBy similarityAdd BLAST | 30 | |
Transmembranei | 523 – 545 | Helical; Name=11By similarityAdd BLAST | 23 | |
Topological domaini | 546 – 548 | ExtracellularBy similarity | 3 | |
Transmembranei | 549 – 569 | Helical; Name=12By similarityAdd BLAST | 21 | |
Topological domaini | 570 – 617 | CytoplasmicBy similarityAdd BLAST | 48 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000214749 | 1 – 617 | Sodium-dependent noradrenaline transporterAdd BLAST | 617 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 176 ↔ 185 | By similarity | ||
Glycosylationi | 184 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 192 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MaxQBi | O55192 |
PaxDbi | O55192 |
PeptideAtlasi | O55192 |
PRIDEi | O55192 |
ProteomicsDBi | 255472 |
PTM databases
GlyGeni | O55192, 2 sites |
iPTMneti | O55192 |
PhosphoSitePlusi | O55192 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000055368, Expressed in placenta and 229 other tissues |
Genevisiblei | O55192, MM |
Interactioni
Subunit structurei
Interacts with PRKCABP.
By similarityGO - Molecular functioni
- actin binding Source: MGI
- alpha-tubulin binding Source: MGI
- beta-tubulin binding Source: MGI
Protein-protein interaction databases
MINTi | O55192 |
STRINGi | 10090.ENSMUSP00000072709 |
Chemistry databases
BindingDBi | O55192 |
Miscellaneous databases
RNActi | O55192, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A2 subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3659, Eukaryota |
GeneTreei | ENSGT00940000158960 |
HOGENOMi | CLU_006855_9_0_1 |
InParanoidi | O55192 |
OMAi | GWLDFIT |
OrthoDBi | 250396at2759 |
TreeFami | TF343812 |
Family and domain databases
InterProi | View protein in InterPro IPR000175, Na/ntran_symport IPR002435, Na/ntran_symport_noradrenaline IPR037272, SNS_sf |
PANTHERi | PTHR11616, PTHR11616, 1 hit |
Pfami | View protein in Pfam PF00209, SNF, 1 hit |
PRINTSi | PR00176, NANEUSMPORT PR01201, NORTRANSPORT |
SUPFAMi | SSF161070, SSF161070, 1 hit |
PROSITEi | View protein in PROSITE PS00610, NA_NEUROTRAN_SYMP_1, 1 hit PS00754, NA_NEUROTRAN_SYMP_2, 1 hit PS50267, NA_NEUROTRAN_SYMP_3, 1 hit |
i Sequence
Sequence statusi: Complete.
O55192-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLLARMNPQV QPELGGADPL PEQPLRPCKT ADLLVVKERN GVQCLLASQD
60 70 80 90 100
SDAQPRETWG KKIDFLLSVV GFAVDLANVW RFPYLCYKNG GGAFLIPYTL
110 120 130 140 150
FLIIAGMPLF YMELALGQYN REGAATVWKI CPFFKGVGYA VILIALYVGF
160 170 180 190 200
YYNVIIAWSL YYLFASFTLN LPWTNCGHSW NSPNCTDPKL LNASVLGDHT
210 220 230 240 250
KYSKYKFTPA AEFYERGVLH LHESSGIHDI GLPQWQLLLC LMVVIVVLYF
260 270 280 290 300
SLWKGVKTSG KVVWITATLP YFVLFVLLVH GVTLPGASNG INAYLHIDFY
310 320 330 340 350
RLKEATVWID AATQIFFSLG AGFGVLIAFA SYNKFDNNCY RDALLTSTIN
360 370 380 390 400
CVTSFISGFA IFSILGYMAH EHKVNIEDVA TEGAGLVFIL YPEAISTLSG
410 420 430 440 450
STFWAVLFFL MLLALGLDSS MGGMEAVITG LADDFQVLKR HRKLFTCVVT
460 470 480 490 500
ISTFLLALFC ITKGGIYVLT LLDTFAAGTS ILFAVLMEAI GVSWFYGVDR
510 520 530 540 550
FSNDIQQMMG FKPGLYWRLC WKFVSPAFLL FVVVVSIINF KPLTYDDYTY
560 570 580 590 600
PPWANWVGWG IALSSMILVP AYVIYKFLSI RGSLWERVAY GITPENEHHL
610
VAQRDVRQFQ LRHWLAI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 609 – 612 | FQLR → LPLQ in AAB94302 (PubMed:9603188).Curated | 4 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U76306 mRNA Translation: AAB94302.1 AY525777 mRNA Translation: AAS17725.1 AY525778 mRNA Translation: AAS17726.1 CH466525 Genomic DNA Translation: EDL11093.1 |
CCDSi | CCDS22525.1 |
RefSeqi | NP_033235.3, NM_009209.3 XP_006530856.1, XM_006530793.3 XP_006530857.1, XM_006530794.3 |
Genome annotation databases
Ensembli | ENSMUST00000072939; ENSMUSP00000072709; ENSMUSG00000055368 ENSMUST00000165470; ENSMUSP00000129869; ENSMUSG00000055368 |
GeneIDi | 20538 |
KEGGi | mmu:20538 |
UCSCi | uc009muh.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U76306 mRNA Translation: AAB94302.1 AY525777 mRNA Translation: AAS17725.1 AY525778 mRNA Translation: AAS17726.1 CH466525 Genomic DNA Translation: EDL11093.1 |
CCDSi | CCDS22525.1 |
RefSeqi | NP_033235.3, NM_009209.3 XP_006530856.1, XM_006530793.3 XP_006530857.1, XM_006530794.3 |
3D structure databases
SMRi | O55192 |
ModBasei | Search... |
Protein-protein interaction databases
MINTi | O55192 |
STRINGi | 10090.ENSMUSP00000072709 |
Chemistry databases
BindingDBi | O55192 |
ChEMBLi | CHEMBL2370 |
DrugCentrali | O55192 |
PTM databases
GlyGeni | O55192, 2 sites |
iPTMneti | O55192 |
PhosphoSitePlusi | O55192 |
Proteomic databases
MaxQBi | O55192 |
PaxDbi | O55192 |
PeptideAtlasi | O55192 |
PRIDEi | O55192 |
ProteomicsDBi | 255472 |
Protocols and materials databases
Antibodypediai | 1363, 138 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000072939; ENSMUSP00000072709; ENSMUSG00000055368 ENSMUST00000165470; ENSMUSP00000129869; ENSMUSG00000055368 |
GeneIDi | 20538 |
KEGGi | mmu:20538 |
UCSCi | uc009muh.2, mouse |
Organism-specific databases
CTDi | 6530 |
MGIi | MGI:1270850, Slc6a2 |
Phylogenomic databases
eggNOGi | KOG3659, Eukaryota |
GeneTreei | ENSGT00940000158960 |
HOGENOMi | CLU_006855_9_0_1 |
InParanoidi | O55192 |
OMAi | GWLDFIT |
OrthoDBi | 250396at2759 |
TreeFami | TF343812 |
Enzyme and pathway databases
Reactomei | R-MMU-442660, Na+/Cl- dependent neurotransmitter transporters |
Miscellaneous databases
BioGRID-ORCSi | 20538, 1 hit in 52 CRISPR screens |
ChiTaRSi | Slc6a2, mouse |
PROi | PR:O55192 |
RNActi | O55192, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000055368, Expressed in placenta and 229 other tissues |
Genevisiblei | O55192, MM |
Family and domain databases
InterProi | View protein in InterPro IPR000175, Na/ntran_symport IPR002435, Na/ntran_symport_noradrenaline IPR037272, SNS_sf |
PANTHERi | PTHR11616, PTHR11616, 1 hit |
Pfami | View protein in Pfam PF00209, SNF, 1 hit |
PRINTSi | PR00176, NANEUSMPORT PR01201, NORTRANSPORT |
SUPFAMi | SSF161070, SSF161070, 1 hit |
PROSITEi | View protein in PROSITE PS00610, NA_NEUROTRAN_SYMP_1, 1 hit PS00754, NA_NEUROTRAN_SYMP_2, 1 hit PS50267, NA_NEUROTRAN_SYMP_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SC6A2_MOUSE | |
Accessioni | O55192Primary (citable) accession number: O55192 Secondary accession number(s): Q6QU62 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | July 27, 2011 | |
Last modified: | April 7, 2021 | |
This is version 150 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families