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Protein

Eukaryotic translation initiation factor 6

Gene

Eif6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-dependent protein kinase C activity. In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR (PubMed:26383020). Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis (PubMed:26391622). Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC (By similarity). Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth (PubMed:21665150).UniRule annotation4 Publications

GO - Molecular functioni

  • ribosomal large subunit binding Source: GO_Central
  • ribosome binding Source: UniProtKB
  • translation initiation factor activity Source: UniProtKB-UniRule

GO - Biological processi

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis, Ribosome biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 6UniRule annotation
Short name:
eIF-6UniRule annotation
Alternative name(s):
B4 integrin interactor
CAB
p27(BBP)
Gene namesi
Name:Eif6
Synonyms:Itgb4bp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1196288 Eif6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality. Embryos die at preimplantation stage.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi235S → A: Abolishes insulin-stimulated translation, abrogates tumorigenesis. 3 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001537351 – 245Eukaryotic translation initiation factor 6Add BLAST245

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei113PhosphotyrosineBy similarity1
Modified residuei165PhosphothreonineUniRule annotation1 Publication1
Modified residuei166PhosphoserineUniRule annotation1 Publication1
Modified residuei174Phosphoserine; by CK1UniRule annotation1 Publication1
Modified residuei175Phosphoserine; by CK1UniRule annotation1 Publication1
Modified residuei235Phosphoserine; by PKCUniRule annotation1 Publication1
Modified residuei239PhosphoserineUniRule annotationBy similarity1
Modified residuei243PhosphoserineUniRule annotationBy similarity1

Post-translational modificationi

Phosphorylation at Ser-174 and Ser-175 by CSNK1D/CK1 promotes nuclear export.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO55135
PaxDbiO55135
PeptideAtlasiO55135
PRIDEiO55135

PTM databases

iPTMnetiO55135
PhosphoSitePlusiO55135
SwissPalmiO55135

Expressioni

Tissue specificityi

Detected in bladder, duodenum, liver, esophagus, pancreas, adipose tissue, megakaryocytes and testis with lower levels in muscle (at protein level).3 Publications

Gene expression databases

BgeeiENSMUSG00000027613 Expressed in 296 organ(s), highest expression level in jejunum
CleanExiMM_EIF6
ExpressionAtlasiO55135 baseline and differential
GenevisibleiO55135 MM

Interactioni

Subunit structurei

Monomer. Associates with the 60S ribosomal subunit. Interacts with RACK1. Interacts with DICER1, AGO2, TARBP2, MOV10 and RPL7A; they form a large RNA-induced silencing complex (RISC).UniRule annotation

Protein-protein interaction databases

BioGridi200831, 7 interactors
IntActiO55135, 4 interactors
STRINGi10090.ENSMUSP00000029142

Structurei

3D structure databases

ProteinModelPortaliO55135
SMRiO55135
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eIF-6 family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3185 Eukaryota
COG1976 LUCA
GeneTreeiENSGT00390000015972
HOGENOMiHOG000230605
HOVERGENiHBG001300
InParanoidiO55135
KOiK03264
OMAiNDWCAFT
OrthoDBiEOG091G0HYF
PhylomeDBiO55135
TreeFamiTF105396

Family and domain databases

CDDicd00527 IF6, 1 hit
HAMAPiMF_00032 eIF_6, 1 hit
InterProiView protein in InterPro
IPR002769 eIF6
PANTHERiPTHR10784 PTHR10784, 1 hit
PfamiView protein in Pfam
PF01912 eIF-6, 1 hit
PIRSFiPIRSF006413 IF-6, 1 hit
SMARTiView protein in SMART
SM00654 eIF6, 1 hit
TIGRFAMsiTIGR00323 eIF-6, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

O55135-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAVRASFENN CEVGCFAKLT NAYCLVAIGG SENFYSVFEG ELSDAIPVVH
60 70 80 90 100
ASIAGCRIIG RMCVGNRHGL LVPNNTTDQE LQHIRNSLPD SVQIRRVEER
110 120 130 140 150
LSALGNVTTC NDYVALVHPD LDRETEEILA DVLKVEVFRQ TVADQVLVGS
160 170 180 190 200
YCVFSNQGGL VHPKTSIEDQ DELSSLLQVP LVAGTVNRGS EVIAAGMVVN
210 220 230 240
DWCAFCGLDT TSTELSVVES VFKLNEAKPS TIATSMRDSL IDSLT
Length:245
Mass (Da):26,511
Last modified:May 30, 2000 - v2
Checksum:i35BF0F4C17D5E045
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A6PWZ2A6PWZ2_MOUSE
Eukaryotic translation initiation f...
Eif6
58Annotation score:
B1AZQ4B1AZQ4_MOUSE
Eukaryotic translation initiation f...
Eif6
81Annotation score:
D6RJJ3D6RJJ3_MOUSE
Eukaryotic translation initiation f...
Eif6
71Annotation score:
D6RG53D6RG53_MOUSE
Eukaryotic translation initiation f...
Eif6
59Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047046 mRNA Translation: AAD28078.1
BC015274 mRNA Translation: AAH15274.1
BC024442 mRNA Translation: AAH24442.1
Y11460 mRNA Translation: CAA72246.1
CCDSiCCDS16956.1
RefSeqiNP_034709.1, NM_010579.2
UniGeneiMm.271674

Genome annotation databases

EnsembliENSMUST00000029142; ENSMUSP00000029142; ENSMUSG00000027613
GeneIDi16418
KEGGimmu:16418
UCSCiuc008nll.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047046 mRNA Translation: AAD28078.1
BC015274 mRNA Translation: AAH15274.1
BC024442 mRNA Translation: AAH24442.1
Y11460 mRNA Translation: CAA72246.1
CCDSiCCDS16956.1
RefSeqiNP_034709.1, NM_010579.2
UniGeneiMm.271674

3D structure databases

ProteinModelPortaliO55135
SMRiO55135
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200831, 7 interactors
IntActiO55135, 4 interactors
STRINGi10090.ENSMUSP00000029142

PTM databases

iPTMnetiO55135
PhosphoSitePlusiO55135
SwissPalmiO55135

Proteomic databases

EPDiO55135
PaxDbiO55135
PeptideAtlasiO55135
PRIDEiO55135

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029142; ENSMUSP00000029142; ENSMUSG00000027613
GeneIDi16418
KEGGimmu:16418
UCSCiuc008nll.1 mouse

Organism-specific databases

CTDi3692
MGIiMGI:1196288 Eif6

Phylogenomic databases

eggNOGiKOG3185 Eukaryota
COG1976 LUCA
GeneTreeiENSGT00390000015972
HOGENOMiHOG000230605
HOVERGENiHBG001300
InParanoidiO55135
KOiK03264
OMAiNDWCAFT
OrthoDBiEOG091G0HYF
PhylomeDBiO55135
TreeFamiTF105396

Miscellaneous databases

PROiPR:O55135
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027613 Expressed in 296 organ(s), highest expression level in jejunum
CleanExiMM_EIF6
ExpressionAtlasiO55135 baseline and differential
GenevisibleiO55135 MM

Family and domain databases

CDDicd00527 IF6, 1 hit
HAMAPiMF_00032 eIF_6, 1 hit
InterProiView protein in InterPro
IPR002769 eIF6
PANTHERiPTHR10784 PTHR10784, 1 hit
PfamiView protein in Pfam
PF01912 eIF-6, 1 hit
PIRSFiPIRSF006413 IF-6, 1 hit
SMARTiView protein in SMART
SM00654 eIF6, 1 hit
TIGRFAMsiTIGR00323 eIF-6, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIF6_MOUSE
AccessioniPrimary (citable) accession number: O55135
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 30, 2000
Last modified: November 7, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Translation initiation factors
    List of translation initiation factor entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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