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Protein

Protocadherin-12

Gene

Pcdh12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cellular adhesion molecule that may play an important role in cell-cell interactions at interendothelial junctions (PubMed:9651350). Acts as a regulator of cell migration, probably via increasing cell-cell adhesion (By similarity). Promotes homotypic calcium-dependent aggregation and adhesion and clusters at intercellular junctions (PubMed:9651350). Unable to bind to catenins, weakly associates with the cytoskeleton (PubMed:9651350).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules Source: MGI
  • cell adhesion Source: GO_Central
  • glycogen metabolic process Source: MGI
  • homophilic cell adhesion via plasma membrane adhesion molecules Source: MGI
  • labyrinthine layer development Source: MGI

Keywordsi

Biological processCell adhesion
LigandCalcium

Names & Taxonomyi

Protein namesi
Recommended name:
Protocadherin-12Curated
Alternative name(s):
Vascular cadherin-21 Publication
Vascular endothelial cadherin-21 Publication
Short name:
VE-cad-21 Publication
Short name:
VE-cadherin-21 Publication
Cleaved into the following chain:
Protocadherin-12, secreted formBy similarity
Gene namesi
Name:Pcdh12Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1855700 Pcdh12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini18 – 716ExtracellularSequence analysisAdd BLAST699
Transmembranei717 – 737HelicalSequence analysisAdd BLAST21
Topological domaini738 – 1180CytoplasmicSequence analysisAdd BLAST443

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Secreted

Pathology & Biotechi

Disruption phenotypei

Mice are viable and fertile (PubMed:15541725, PubMed:18477666). Mice however show alterations in placental development that result in embryonic growth retardation: placentas are smaller and show limited angiogenesis and mis-segregation of the labyrinthine and intermediate layers (PubMed:18477666). Mice also display modifications in the structure and function of arteries, such as rearrangement of the arterial wall elastic fibers (PubMed:22205043).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000000399718 – 1180Protocadherin-12Add BLAST1163
ChainiPRO_000044404218 – ?Protocadherin-12, secreted formBy similarity

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi265N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi415N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi582N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi659N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi662N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei859PhosphoserineCombined sources1
Modified residuei1064PhosphoserineBy similarity1

Post-translational modificationi

N-glycosylated.1 Publication
Protocadherin-12: Cleaved by ADAM10 close to the transmembrane domain to release the Protocadherin-12, secreted form in the serum. Cleavage results in reduced cellular adhesion in a cell migration assay.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiO55134
PaxDbiO55134
PRIDEiO55134

PTM databases

iPTMnetiO55134
PhosphoSitePlusiO55134

Expressioni

Tissue specificityi

Expressed in endothelial cells: localizes in vasculogenic rather than angiogenic endothelium (PubMed:9651350, PubMed:15541725). Strongly expressed in a subset of invasive cells of the placenta, named glycogen-rich trophoblasts cells (at protein level) (PubMed:15541725). glycogen-rich trophoblasts cells originate from the from the ectoplacental cone where they rapidly form tight islets (at protein level) (PubMed:16269175). In adult mice, present at high level in mesangial cells of kidney glomeruli, while expression was not detected in other types of perivascular cells (PubMed:15541725).3 Publications

Gene expression databases

BgeeiENSMUSG00000024440 Expressed in 144 organ(s), highest expression level in decidua
CleanExiMM_PCDH12
ExpressionAtlasiO55134 baseline and differential
GenevisibleiO55134 MM

Interactioni

Protein-protein interaction databases

IntActiO55134, 1 interactor
STRINGi10090.ENSMUSP00000025311

Structurei

3D structure databases

ProteinModelPortaliO55134
SMRiO55134
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 135Cadherin 1PROSITE-ProRule annotationAdd BLAST108
Domaini136 – 244Cadherin 2PROSITE-ProRule annotationAdd BLAST109
Domaini245 – 352Cadherin 3PROSITE-ProRule annotationAdd BLAST108
Domaini355 – 460Cadherin 4PROSITE-ProRule annotationAdd BLAST106
Domaini461 – 565Cadherin 5PROSITE-ProRule annotationAdd BLAST105
Domaini600 – 711Cadherin 6PROSITE-ProRule annotationAdd BLAST112

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3594 Eukaryota
ENOG410XQHI LUCA
GeneTreeiENSGT00900000140770
HOGENOMiHOG000220893
HOVERGENiHBG101142
InParanoidiO55134
KOiK16499
OMAiWELEIVV
OrthoDBiEOG091G00QD
TreeFamiTF352008

Family and domain databases

InterProiView protein in InterPro
IPR002126 Cadherin-like_dom
IPR015919 Cadherin-like_sf
IPR020894 Cadherin_CS
IPR013164 Cadherin_N
IPR030720 Protocadherin-12
PANTHERiPTHR24028:SF42 PTHR24028:SF42, 1 hit
PfamiView protein in Pfam
PF00028 Cadherin, 4 hits
PF08266 Cadherin_2, 1 hit
PRINTSiPR00205 CADHERIN
SMARTiView protein in SMART
SM00112 CA, 6 hits
SUPFAMiSSF49313 SSF49313, 5 hits
PROSITEiView protein in PROSITE
PS00232 CADHERIN_1, 5 hits
PS50268 CADHERIN_2, 6 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O55134-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MMLLLPFLLG LLGPGSYLFI SGDCQEVATV MVKFQVTEEV PSGTVIGKLS
60 70 80 90 100
QELRVEERRG KAGDAFQILQ LPQALPVQMN SEDGLLSTSS RLDREKLCRQ
110 120 130 140 150
EDPCLVSFDV LATGASALIH VEIQVLDIND HQPQFPKDEQ ELEISESASL
160 170 180 190 200
HTRIPLDRAL DQDTGPNSLY SYSLSPSEHF ALDVIVGPDE TKHAELVVVK
210 220 230 240 250
ELDRELHSYF DLVLTAYDNG NPPKSGISVV KVNVLDSNDN SPVFAESSLA
260 270 280 290 300
LEIPEDTVPG TLLINLTATD PDQGPNGEVE FFFGKHVSPE VMNTFGIDAK
310 320 330 340 350
TGQIILRQAL DYEKNPAYEV DVQARDLGPN SIPGHCKVLI KVLDVNDNAP
360 370 380 390 400
SILITWASQT SLVSEDLPRD SFIALVSAND LDSGNNGLVH CWLNQELGHF
410 420 430 440 450
RLKRTNGNTY MLLTNATLDR EQWPIYTLTV FAQDQGPQPL SAEKELQIQV
460 470 480 490 500
SDVNDNAPVF EKSRYEVSTW ENNPPSLHLI TLKAHDADLG SNGKVSYRIK
510 520 530 540 550
DSPVSHLVII DFETGEVTAQ RSLDYEQMAG FEFQVIAEDR GQPQLASSIS
560 570 580 590 600
VWVSLLDAND NAPEVIQPVL SEGKATLSVL VNASTGHLLL PIENPSGMDP
610 620 630 640 650
AGTGIPPKAT HSPWSFLLLT IVARDADSGA NGELFYSIQS GNDAHLFFLS
660 670 680 690 700
PSLGQLFINV TNASSLIGSQ WDLGIVVEDQ GSPSLQTQVS LKVVFVTSVD
710 720 730 740 750
HLRDSAHEPG VLSTPALALI CLAVLLAIFG LLLALFVSIC RTERKDNRAY
760 770 780 790 800
NCREAESSYR HQPKRPQKHI QKADIHLVPV LRAHENETDE VRPSHKDTSK
810 820 830 840 850
ETLMEAGWDS CLQAPFHLTP TLYRTLRNQG NQGELAESQE VLQDTFNFLF
860 870 880 890 900
NHPRQRNASR ENLNLPESPP AVRQPLLRPL KVPGSPIARA TGDQDKEEAP
910 920 930 940 950
QSPPASSATL RRQRNFNGKV SPRGESGPHQ ILRSLVRLSV AAFAERNPVE
960 970 980 990 1000
EPAGDSPPVQ QISQLLSLLH QGQFQPKPNH RGNKYLAKPG GSSRGTIPDT
1010 1020 1030 1040 1050
EGLVGLKPSG QAEPDLEEGP PSPEEDLSVK RLLEEELSSL LDPNTGLALD
1060 1070 1080 1090 1100
KLSPPDPAWM ARLSLPLTTN YRDNLSSPDA TTSEEPRTFQ TFGKTVGPGP
1110 1120 1130 1140 1150
ELSPTGTRLA STFVSEMSSL LEMLLGQHTV PVEAASAALR RLSVCGRTLS
1160 1170 1180
LDLATSGASA SEAQGRKKAA ESRLGCGRNL
Length:1,180
Mass (Da):128,673
Last modified:September 18, 2013 - v2
Checksum:i1E2BCC09DFCA4085
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0JNZ2A0JNZ2_MOUSE
Pcdh12 protein
Pcdh12 mCG_18330
121Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti813Q → E in CAA69965 (PubMed:9651350).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08715 mRNA Translation: CAA69965.1
AC133646 Genomic DNA No translation available.
AC152450 Genomic DNA No translation available.
CH466528 Genomic DNA Translation: EDL10091.1
CCDSiCCDS29199.1
PIRiT31066
RefSeqiNP_059074.2, NM_017378.2
UniGeneiMm.12862

Genome annotation databases

EnsembliENSMUST00000025311; ENSMUSP00000025311; ENSMUSG00000024440
GeneIDi53601
KEGGimmu:53601
UCSCiuc008esb.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08715 mRNA Translation: CAA69965.1
AC133646 Genomic DNA No translation available.
AC152450 Genomic DNA No translation available.
CH466528 Genomic DNA Translation: EDL10091.1
CCDSiCCDS29199.1
PIRiT31066
RefSeqiNP_059074.2, NM_017378.2
UniGeneiMm.12862

3D structure databases

ProteinModelPortaliO55134
SMRiO55134
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO55134, 1 interactor
STRINGi10090.ENSMUSP00000025311

PTM databases

iPTMnetiO55134
PhosphoSitePlusiO55134

Proteomic databases

MaxQBiO55134
PaxDbiO55134
PRIDEiO55134

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025311; ENSMUSP00000025311; ENSMUSG00000024440
GeneIDi53601
KEGGimmu:53601
UCSCiuc008esb.1 mouse

Organism-specific databases

CTDi51294
MGIiMGI:1855700 Pcdh12

Phylogenomic databases

eggNOGiKOG3594 Eukaryota
ENOG410XQHI LUCA
GeneTreeiENSGT00900000140770
HOGENOMiHOG000220893
HOVERGENiHBG101142
InParanoidiO55134
KOiK16499
OMAiWELEIVV
OrthoDBiEOG091G00QD
TreeFamiTF352008

Miscellaneous databases

PROiPR:O55134
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024440 Expressed in 144 organ(s), highest expression level in decidua
CleanExiMM_PCDH12
ExpressionAtlasiO55134 baseline and differential
GenevisibleiO55134 MM

Family and domain databases

InterProiView protein in InterPro
IPR002126 Cadherin-like_dom
IPR015919 Cadherin-like_sf
IPR020894 Cadherin_CS
IPR013164 Cadherin_N
IPR030720 Protocadherin-12
PANTHERiPTHR24028:SF42 PTHR24028:SF42, 1 hit
PfamiView protein in Pfam
PF00028 Cadherin, 4 hits
PF08266 Cadherin_2, 1 hit
PRINTSiPR00205 CADHERIN
SMARTiView protein in SMART
SM00112 CA, 6 hits
SUPFAMiSSF49313 SSF49313, 5 hits
PROSITEiView protein in PROSITE
PS00232 CADHERIN_1, 5 hits
PS50268 CADHERIN_2, 6 hits
ProtoNetiSearch...

Entry informationi

Entry nameiPCD12_MOUSE
AccessioniPrimary (citable) accession number: O55134
Secondary accession number(s): G5E847
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: September 18, 2013
Last modified: November 7, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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