UniProtKB - O55127 (CP26A_MOUSE)
Protein
Cytochrome P450 26A1
Gene
Cyp26a1
Organism
Mus musculus (Mouse)
Status
Functioni
A cytochrome P450 monooxygenase involved in the metabolism of all-trans retinoic acid (atRA), a signaling molecule that binds to retinoic acid receptors and regulates gene transcription. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon hydrogen bonds of atRA primarily at C-4 and C-18 (PubMed:9250660, PubMed:9442090). Has no activity toward 9-cis and 13-cis retinoic acid stereoisomers. May play a role in the oxidative metabolism of xenobiotics such as tazarotenic acid (By similarity).By similarity2 Publications
Catalytic activityi
- all-trans-retinoate + O2 + reduced [NADPH—hemoprotein reductase] = all-trans-(4S)-hydroxyretinoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]2 PublicationsThis reaction proceeds in the forward2 Publications direction.
- all-trans-(4S)-hydroxyretinoate + O2 + reduced [NADPH—hemoprotein reductase] = all-trans-(4S,16)-dihydroxyretinoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
- all-trans-retinoate + O2 + reduced [NADPH—hemoprotein reductase] = all-trans-18-hydroxyretinoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]1 PublicationThis reaction proceeds in the forward1 Publication direction.
Cofactori
hemeBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 442 | Iron (heme axial ligand)Sequence analysis | 1 |
GO - Molecular functioni
- all-trans retinoic acid 18-hydroxylase activity Source: MGI
- heme binding Source: InterPro
- iron ion binding Source: InterPro
- monooxygenase activity Source: GO_Central
- oxidoreductase activity Source: GO_Central
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen Source: UniProtKB
- retinoic acid 4-hydroxylase activity Source: UniProtKB
- retinoic acid binding Source: MGI
GO - Biological processi
- anterior/posterior pattern specification Source: MGI
- cellular response to retinoic acid Source: UniProtKB
- central nervous system development Source: MGI
- neural crest cell development Source: MGI
- oxidation-reduction process Source: GO_Central
- retinoic acid catabolic process Source: MGI
- retinoic acid metabolic process Source: UniProtKB
- retinoic acid receptor signaling pathway Source: MGI
- sterol metabolic process Source: GO_Central
- xenobiotic metabolic process Source: UniProtKB
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-211916, Vitamins R-MMU-5365859, RA biosynthesis pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome P450 26A1 (EC:1.14.13.-2 Publications)Alternative name(s): Cytochrome P450RAI1 Publication Retinoic acid 4-hydroxylase1 Publication Retinoic acid-metabolizing cytochrome1 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1096359, Cyp26a1 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Peripheral membrane protein
- Microsome membrane By similarity; Peripheral membrane protein
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051981 | 1 – 497 | Cytochrome P450 26A1Add BLAST | 497 |
Proteomic databases
MaxQBi | O55127 |
PaxDbi | O55127 |
PRIDEi | O55127 |
PTM databases
iPTMneti | O55127 |
PhosphoSitePlusi | O55127 |
Expressioni
Inductioni
By retinoic acid.2 Publications
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000025946 |
Miscellaneous databases
RNActi | O55127, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the cytochrome P450 family.Curated
Phylogenomic databases
eggNOGi | KOG0157, Eukaryota |
InParanoidi | O55127 |
OrthoDBi | 871849at2759 |
PhylomeDBi | O55127 |
TreeFami | TF105093 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002403, Cyt_P450_E_grp-IV IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00465, EP450IV PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O55127-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGLPALLASA LCTFVLPLLL FLAALKLWDL YCVSSRDRSC ALPLPPGTMG
60 70 80 90 100
FPFFGETLQM VLQRRKFLQM KRRKYGFIYK THLFGRPTVR VMGADNVRRI
110 120 130 140 150
LLGEHRLVSV HWPASVRTIL GAGCLSNLHD SSHKQRKKVI MQAFSREALQ
160 170 180 190 200
CYVLVIAEEV SSCLEQWLSC GERGLLVYPE VKRLMFRIAM RILLGCEPGP
210 220 230 240 250
AGGGEDEQQL VEAFEEMTRN LFSLPIDVPF SGLYRGVKAR NLIHARIEEN
260 270 280 290 300
IRAKIRRLQA TEPDGGCKDA LQLLIEHSWE RGERLDMQAL KQSSTELLFG
310 320 330 340 350
GHETTASAAT SLITYLGLYP HVLQKVREEI KSKGLLCKSN QDNKLDMETL
360 370 380 390 400
EQLKYIGCVI KETLRLNPPV PGGFRVALKT FELNGYQIPK GWNVIYSICD
410 420 430 440 450
THDVADIFTN KEEFNPDRFI VPHPEDASRF SFIPFGGGLR SCVGKEFAKI
460 470 480 490
LLKIFTVELA RHCDWQLLNG PPTMKTSPTV YPVDNLPARF TYFQGDI
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0R4J061 | A0A0R4J061_MOUSE | Cytochrome P450 26A1 | Cyp26a1 | 497 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 9 | S → T in AAD17217 (PubMed:11063033).Curated | 1 | |
Sequence conflicti | 154 | L → P in AAH12673 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 356 | I → T in AAH12673 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 492 | Y → H in AAH12673 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y12657 mRNA Translation: CAA73206.1 AF115769 mRNA Translation: AAD17217.1 BC012673 mRNA Translation: AAH12673.1 |
CCDSi | CCDS29780.1 |
RefSeqi | NP_031837.2, NM_007811.2 |
Genome annotation databases
GeneIDi | 13082 |
KEGGi | mmu:13082 |
UCSCi | uc008his.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y12657 mRNA Translation: CAA73206.1 AF115769 mRNA Translation: AAD17217.1 BC012673 mRNA Translation: AAH12673.1 |
CCDSi | CCDS29780.1 |
RefSeqi | NP_031837.2, NM_007811.2 |
3D structure databases
SMRi | O55127 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000025946 |
PTM databases
iPTMneti | O55127 |
PhosphoSitePlusi | O55127 |
Proteomic databases
MaxQBi | O55127 |
PaxDbi | O55127 |
PRIDEi | O55127 |
Genome annotation databases
GeneIDi | 13082 |
KEGGi | mmu:13082 |
UCSCi | uc008his.2, mouse |
Organism-specific databases
CTDi | 1592 |
MGIi | MGI:1096359, Cyp26a1 |
Phylogenomic databases
eggNOGi | KOG0157, Eukaryota |
InParanoidi | O55127 |
OrthoDBi | 871849at2759 |
PhylomeDBi | O55127 |
TreeFami | TF105093 |
Enzyme and pathway databases
Reactomei | R-MMU-211916, Vitamins R-MMU-5365859, RA biosynthesis pathway |
Miscellaneous databases
BioGRID-ORCSi | 13082, 3 hits in 18 CRISPR screens |
PROi | PR:O55127 |
RNActi | O55127, protein |
SOURCEi | Search... |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002403, Cyt_P450_E_grp-IV IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00465, EP450IV PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CP26A_MOUSE | |
Accessioni | O55127Primary (citable) accession number: O55127 Secondary accession number(s): Q9R1F4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | June 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families