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Protein

Stromelysin-2

Gene

Mmp10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.

Catalytic activityi

Similar to stromelysin 1, but action on collagen types III, IV and V is weak.

Cofactori

Protein has several cofactor binding sites:
  • Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity
  • Ca2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi92Zinc; in inhibited formBy similarity1
Metal bindingi168Zinc 1By similarity1
Metal bindingi170Zinc 1By similarity1
Metal bindingi183Zinc 1By similarity1
Metal bindingi196Zinc 1By similarity1
Metal bindingi218Zinc 2; catalyticBy similarity1
Active sitei219PROSITE-ProRule annotation1
Metal bindingi222Zinc 2; catalyticBy similarity1
Metal bindingi228Zinc 2; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

  • collagen catabolic process Source: UniProtKB-KW
  • regulation of cell migration Source: MGI

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
Biological processCollagen degradation
LigandCalcium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-1442490 Collagen degradation
R-MMU-1474228 Degradation of the extracellular matrix
R-MMU-1592389 Activation of Matrix Metalloproteinases

Protein family/group databases

MEROPSiM10.011

Names & Taxonomyi

Protein namesi
Recommended name:
Stromelysin-2 (EC:3.4.24.22)
Short name:
SL-2
Alternative name(s):
Matrix metalloproteinase-10
Short name:
MMP-10
Transin-2
Gene namesi
Name:Mmp10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:97007 Mmp10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17CuratedAdd BLAST17
PropeptideiPRO_000002876618 – 99Activation peptideBy similarityAdd BLAST82
ChainiPRO_0000028767100 – 476Stromelysin-2Add BLAST377

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi289 ↔ 476By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

MaxQBiO55123
PaxDbiO55123
PRIDEiO55123

PTM databases

iPTMnetiO55123
PhosphoSitePlusiO55123

Expressioni

Tissue specificityi

Expressed in small intestine. Weak levels in heart and lung.

Inductioni

By wounding.

Gene expression databases

BgeeiENSMUSG00000047562 Expressed in 28 organ(s), highest expression level in colon
CleanExiMM_MMP10
ExpressionAtlasiO55123 baseline and differential
GenevisibleiO55123 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034488

Structurei

3D structure databases

ProteinModelPortaliO55123
SMRiO55123
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati286 – 335Hemopexin 1Add BLAST50
Repeati336 – 382Hemopexin 2Add BLAST47
Repeati384 – 432Hemopexin 3Add BLAST49
Repeati433 – 476Hemopexin 4Add BLAST44

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi90 – 97Cysteine switchBy similarity8

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Belongs to the peptidase M10A family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG1565 Eukaryota
ENOG410XQ5D LUCA
GeneTreeiENSGT00760000118870
HOGENOMiHOG000217927
HOVERGENiHBG052484
InParanoidiO55123
KOiK01396
OMAiSNSWLLC
OrthoDBiEOG091G03DP
PhylomeDBiO55123
TreeFamiTF315428

Family and domain databases

CDDicd00094 HX, 1 hit
cd04278 ZnMc_MMP, 1 hit
Gene3Di2.110.10.10, 1 hit
3.40.390.10, 1 hit
InterProiView protein in InterPro
IPR000585 Hemopexin-like_dom
IPR036375 Hemopexin-like_dom_sf
IPR018487 Hemopexin-like_repeat
IPR018486 Hemopexin_CS
IPR033739 M10A_MMP
IPR024079 MetalloPept_cat_dom_sf
IPR001818 Pept_M10_metallopeptidase
IPR021190 Pept_M10A
IPR021158 Pept_M10A_Zn_BS
IPR006026 Peptidase_Metallo
IPR002477 Peptidoglycan-bd-like
IPR036365 PGBD-like_sf
PfamiView protein in Pfam
PF00045 Hemopexin, 4 hits
PF00413 Peptidase_M10, 1 hit
PF01471 PG_binding_1, 1 hit
PIRSFiPIRSF001191 Peptidase_M10A_matrix, 1 hit
PRINTSiPR00138 MATRIXIN
SMARTiView protein in SMART
SM00120 HX, 4 hits
SM00235 ZnMc, 1 hit
SUPFAMiSSF47090 SSF47090, 1 hit
SSF50923 SSF50923, 1 hit
PROSITEiView protein in PROSITE
PS00546 CYSTEINE_SWITCH, 1 hit
PS00024 HEMOPEXIN, 1 hit
PS51642 HEMOPEXIN_2, 4 hits
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O55123-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEPLAILALL SLPICSAYPL HGAVTQGHPS MDLAQQYLEK YYNFKKNEKQ
60 70 80 90 100
IFKRKDSSPV VKKIQEMQKF LGLEMTGKLD SNTMELMHKP RCGVPDVGGF
110 120 130 140 150
STFPGSPKWR KSHITYRIVN YTPDLPRQSV DSAIEKALKV WEEVTPLTFS
160 170 180 190 200
RISEGEADIM ISFAVGEHGD FYPFDGPGQS LAHAYPPGPG FYGDVHFDDD
210 220 230 240 250
EKWTLAPSGT NLFLVAAHEL GHSLGLFHSD KKESLMYPVY RFSTSPANFH
260 270 280 290 300
LSQDDIEGIQ SLYGAGPSSD ATVVPVLSVS PRPETPDKCD PALSFDSVST
310 320 330 340 350
LRGEVLFFKD RYFWRRSHWN PEPEFHLISA FWPTLPSDLD AAYEAHNTDS
360 370 380 390 400
VLIFKGSQFW AVRGNEVQAG YPKGIHTLGF PPTVKKIDAA VFEKEKKKTY
410 420 430 440 450
FFVGDKYWRF DETRHVMDKG FPRQITDDFP GIEPQVDAVL HEFGFFYFFR
460 470
GSSQFEFDPN ARTVTHILKS NSWLLC
Length:476
Mass (Da):53,911
Last modified:June 1, 1998 - v1
Checksum:i2EB1CC41468F0AC6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13185 mRNA Translation: CAA73641.1
AK020292 mRNA Translation: BAB32058.1
CCDSiCCDS22807.1
PIRiJC6505
RefSeqiNP_062344.1, NM_019471.3
UniGeneiMm.14126

Genome annotation databases

EnsembliENSMUST00000034488; ENSMUSP00000034488; ENSMUSG00000047562
GeneIDi17384
KEGGimmu:17384
UCSCiuc009ocq.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13185 mRNA Translation: CAA73641.1
AK020292 mRNA Translation: BAB32058.1
CCDSiCCDS22807.1
PIRiJC6505
RefSeqiNP_062344.1, NM_019471.3
UniGeneiMm.14126

3D structure databases

ProteinModelPortaliO55123
SMRiO55123
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034488

Protein family/group databases

MEROPSiM10.011

PTM databases

iPTMnetiO55123
PhosphoSitePlusiO55123

Proteomic databases

MaxQBiO55123
PaxDbiO55123
PRIDEiO55123

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034488; ENSMUSP00000034488; ENSMUSG00000047562
GeneIDi17384
KEGGimmu:17384
UCSCiuc009ocq.2 mouse

Organism-specific databases

CTDi4319
MGIiMGI:97007 Mmp10

Phylogenomic databases

eggNOGiKOG1565 Eukaryota
ENOG410XQ5D LUCA
GeneTreeiENSGT00760000118870
HOGENOMiHOG000217927
HOVERGENiHBG052484
InParanoidiO55123
KOiK01396
OMAiSNSWLLC
OrthoDBiEOG091G03DP
PhylomeDBiO55123
TreeFamiTF315428

Enzyme and pathway databases

ReactomeiR-MMU-1442490 Collagen degradation
R-MMU-1474228 Degradation of the extracellular matrix
R-MMU-1592389 Activation of Matrix Metalloproteinases

Miscellaneous databases

PROiPR:O55123
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000047562 Expressed in 28 organ(s), highest expression level in colon
CleanExiMM_MMP10
ExpressionAtlasiO55123 baseline and differential
GenevisibleiO55123 MM

Family and domain databases

CDDicd00094 HX, 1 hit
cd04278 ZnMc_MMP, 1 hit
Gene3Di2.110.10.10, 1 hit
3.40.390.10, 1 hit
InterProiView protein in InterPro
IPR000585 Hemopexin-like_dom
IPR036375 Hemopexin-like_dom_sf
IPR018487 Hemopexin-like_repeat
IPR018486 Hemopexin_CS
IPR033739 M10A_MMP
IPR024079 MetalloPept_cat_dom_sf
IPR001818 Pept_M10_metallopeptidase
IPR021190 Pept_M10A
IPR021158 Pept_M10A_Zn_BS
IPR006026 Peptidase_Metallo
IPR002477 Peptidoglycan-bd-like
IPR036365 PGBD-like_sf
PfamiView protein in Pfam
PF00045 Hemopexin, 4 hits
PF00413 Peptidase_M10, 1 hit
PF01471 PG_binding_1, 1 hit
PIRSFiPIRSF001191 Peptidase_M10A_matrix, 1 hit
PRINTSiPR00138 MATRIXIN
SMARTiView protein in SMART
SM00120 HX, 4 hits
SM00235 ZnMc, 1 hit
SUPFAMiSSF47090 SSF47090, 1 hit
SSF50923 SSF50923, 1 hit
PROSITEiView protein in PROSITE
PS00546 CYSTEINE_SWITCH, 1 hit
PS00024 HEMOPEXIN, 1 hit
PS51642 HEMOPEXIN_2, 4 hits
PS00142 ZINC_PROTEASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMMP10_MOUSE
AccessioniPrimary (citable) accession number: O55123
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: June 1, 1998
Last modified: November 7, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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