UniProtKB - O55074 (AKA7A_MOUSE)
Protein
A-kinase anchor protein 7 isoform alpha
Gene
Akap7
Organism
Mus musculus (Mouse)
Status
Functioni
Targets the cAMP-dependent protein kinase (PKA) to the plasma membrane, and permits functional coupling to the L-type calcium channel. The membrane-associated form reduces epithelial sodium channel (ENaC) activity, whereas the free cytoplasmic form may negatively regulate ENaC channel feedback inhibition by intracellular sodium (By similarity).By similarity1 Publication
GO - Molecular functioni
- AMP binding Source: MGI
- protein C-terminus binding Source: MGI
- protein domain specific binding Source: MGI
- protein kinase A binding Source: UniProtKB
- protein kinase A regulatory subunit binding Source: MGI
- protein kinase binding Source: MGI
GO - Biological processi
- cellular response to cAMP Source: MGI
- modulation of chemical synaptic transmission Source: SynGO
- positive regulation of delayed rectifier potassium channel activity Source: MGI
- positive regulation of potassium ion transmembrane transport Source: MGI
- protein localization Source: MGI
- regulation of membrane repolarization Source: MGI
- regulation of protein kinase A signaling Source: MGI
- transmembrane receptor protein serine/threonine kinase signaling pathway Source: MGI
Names & Taxonomyi
Protein namesi | Recommended name: A-kinase anchor protein 7 isoform alphaShort name: AKAP-7 isoform alpha Alternative name(s): A-kinase anchor protein 18 Short name: AKAP-18 A-kinase anchor protein 9 kDa Short name: AKAP 9 Protein kinase A-anchoring protein 7 isoform alpha Short name: PRKA7 isoform alpha |
Gene namesi | Name:Akap7 Synonyms:Akap18 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1859150, Akap7 |
Subcellular locationi
Plasma membrane
- Lateral cell membrane By similarity; Lipid-anchor 1 Publication
Cytosol
- cytosol Source: MGI
Endoplasmic reticulum
- sarcoplasmic reticulum Source: MGI
Nucleus
- nucleus Source: MGI
Plasma Membrane
- apical plasma membrane Source: MGI
- lateral plasma membrane Source: MGI
- T-tubule Source: MGI
Other locations
- exocytic vesicle Source: MGI
- hippocampal mossy fiber to CA3 synapse Source: SynGO
- membrane Source: MGI
- protein-containing complex Source: MGI
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000064532 | 2 – 81 | A-kinase anchor protein 7 isoform alphaAdd BLAST | 80 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycine1 Publication | 1 | |
Lipidationi | 5 | S-palmitoyl cysteine1 Publication | 1 | |
Lipidationi | 6 | S-palmitoyl cysteine1 Publication | 1 |
Keywords - PTMi
Lipoprotein, Myristate, PalmitateProteomic databases
PeptideAtlasi | O55074 |
PRIDEi | O55074 |
PTM databases
iPTMneti | O55074 |
PhosphoSitePlusi | O55074 |
SwissPalmi | O55074 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000039166, Expressed in cerebral cortex and 249 other tissues |
ExpressionAtlasi | O55074, baseline and differential |
Genevisiblei | O55074, MM |
Interactioni
Subunit structurei
Binds cAMP-dependent protein kinase (PKA).
Interacts with PRKCA; only the cytoplasmic form is capable of interacting with PRKCA (By similarity).
By similarityGO - Molecular functioni
- protein C-terminus binding Source: MGI
- protein domain specific binding Source: MGI
- protein kinase A binding Source: UniProtKB
- protein kinase A regulatory subunit binding Source: MGI
- protein kinase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 240608, 1 interactor |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 11 | Required for membrane localizationBy similarity | 10 | |
Regioni | 29 – 42 | RII-bindingBy similarityAdd BLAST | 14 |
Phylogenomic databases
GeneTreei | ENSGT00390000012756 |
HOGENOMi | CLU_177905_0_0_1 |
OrthoDBi | 606751at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR019511, AKAP7_RI-RII-bd_dom |
Pfami | View protein in Pfam PF10470, AKAP7_RIRII_bdg, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform Alpha (identifier: O55074-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGQLCCFPFA RDEGKICEKD RREPEDAELV RLSKRLVENA VLKAVQQYLE
60 70 80
ETQNKKQPGE GNSTKAEEGD RNGDGSDNNR K
Isoform Gamma (identifier: Q7TN79-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q7TN79.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BJL8 | H3BJL8_MOUSE | A-kinase anchor protein 7 isoform a... | Akap7 | 141 | Annotation score: | ||
Q4KL21 | Q4KL21_MOUSE | A-kinase anchor protein 7 isoform a... | Akap7 | 122 | Annotation score: | ||
H3BJV2 | H3BJV2_MOUSE | A-kinase anchor protein 7 isoform a... | Akap7 | 126 | Annotation score: | ||
A0A1W2P7B3 | A0A1W2P7B3_MOUSE | A-kinase anchor protein 7 isoform a... | Akap7 | 95 | Annotation score: | ||
H3BKK9 | H3BKK9_MOUSE | A-kinase anchor protein 7 isoform a... | Akap7 | 19 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 12 | D → E in AAC40085 (PubMed:9545239).Curated | 1 | |
Sequence conflicti | 22 | R → K in AAC40085 (PubMed:9545239).Curated | 1 | |
Sequence conflicti | 64 | T → V in AAC40085 (PubMed:9545239).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF047716 mRNA Translation: AAC40085.1 AC153549 Genomic DNA No translation available. AC153550 Genomic DNA No translation available. CH466540 Genomic DNA Translation: EDL04795.1 |
CCDSi | CCDS83688.1 [O55074-1] |
RefSeqi | NP_001334389.1, NM_001347460.1 [O55074-1] |
Genome annotation databases
Ensembli | ENSMUST00000100012; ENSMUSP00000097590; ENSMUSG00000039166 [O55074-1] |
GeneIDi | 432442 |
KEGGi | mmu:432442 |
UCSCi | uc007erh.1, mouse [O55074-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF047716 mRNA Translation: AAC40085.1 AC153549 Genomic DNA No translation available. AC153550 Genomic DNA No translation available. CH466540 Genomic DNA Translation: EDL04795.1 |
CCDSi | CCDS83688.1 [O55074-1] |
RefSeqi | NP_001334389.1, NM_001347460.1 [O55074-1] |
3D structure databases
SMRi | O55074 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 240608, 1 interactor |
PTM databases
iPTMneti | O55074 |
PhosphoSitePlusi | O55074 |
SwissPalmi | O55074 |
Proteomic databases
PeptideAtlasi | O55074 |
PRIDEi | O55074 |
Protocols and materials databases
Antibodypediai | 19674, 280 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000100012; ENSMUSP00000097590; ENSMUSG00000039166 [O55074-1] |
GeneIDi | 432442 |
KEGGi | mmu:432442 |
UCSCi | uc007erh.1, mouse [O55074-1] |
Organism-specific databases
CTDi | 9465 |
MGIi | MGI:1859150, Akap7 |
Phylogenomic databases
GeneTreei | ENSGT00390000012756 |
HOGENOMi | CLU_177905_0_0_1 |
OrthoDBi | 606751at2759 |
Miscellaneous databases
BioGRID-ORCSi | 432442, 1 hit in 17 CRISPR screens |
ChiTaRSi | Akap7, mouse |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039166, Expressed in cerebral cortex and 249 other tissues |
ExpressionAtlasi | O55074, baseline and differential |
Genevisiblei | O55074, MM |
Family and domain databases
InterProi | View protein in InterPro IPR019511, AKAP7_RI-RII-bd_dom |
Pfami | View protein in Pfam PF10470, AKAP7_RIRII_bdg, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AKA7A_MOUSE | |
Accessioni | O55074Primary (citable) accession number: O55074 Secondary accession number(s): G3X9Q5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | October 3, 2012 | |
Last modified: | December 2, 2020 | |
This is version 139 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot