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Protein

Serine/threonine-protein kinase tousled-like 2

Gene

Tlk2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation. Phosphorylates the chromatin assembly factors ASF1A AND ASF1B. Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly (By similarity). Negative regulator of amino acid starvation-induced autophagy (By similarity).By similarity
Testis-specific isoforms may play a role in spermatogenesis. Highly expressed in embryos throughout development.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Activity regulationi

Cell cycle-regulated, with maximal activity in the S-phase. Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase, probably by CHEK1, possibly at Ser-696. This inhibition is cell cycle checkpoint- and ATM-dependent (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei437ATPPROSITE-ProRule annotation1
Active sitei538Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi414 – 422ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Differentiation, DNA damage, Spermatogenesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase tousled-like 2 (EC:2.7.11.1)
Alternative name(s):
PKU-alpha
Tousled-like kinase 2
Gene namesi
Name:Tlk2
Synonyms:Tlk
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1346023 Tlk2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867551 – 718Serine/threonine-protein kinase tousled-like 2Add BLAST718

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei73PhosphoserineBy similarity1
Modified residuei102PhosphoserineBy similarity1
Modified residuei696Phosphoserine; by CHEK1By similarity1
Isoform 2Curated (identifier: O55047-2)
Modified residuei94PhosphoserineCombined sourcesCurated1
Modified residuei99PhosphoserineCombined sourcesCurated1

Post-translational modificationi

Phosphorylated at Ser-696, probably by CHEK1.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO55047
MaxQBiO55047
PaxDbiO55047
PeptideAtlasiO55047
PRIDEiO55047

PTM databases

iPTMnetiO55047
PhosphoSitePlusiO55047

Expressioni

Tissue specificityi

Ubiquitously expressed in all tissues examined, with high levels in heart and testis, in particular the pachytene spermatocytes and in round spermatids. Some evidence for the existence of a testis-specific isoform suggesting a role in spermatogenesis.1 Publication3 Publications

Gene expression databases

BgeeiENSMUSG00000020694 Expressed in 287 organ(s), highest expression level in muscle tissue
CleanExiMM_TLK2
ExpressionAtlasiO55047 baseline and differential
GenevisibleiO55047 MM

Interactioni

Subunit structurei

Monomer and heterodimer with TLK1. Interacts with ASF1A and ASF1B. May also interact with FEZ1/LZTS1 and FEZ2 (By similarity). Association with 14-3-3 proteins such as YWHAZ regulates subcellular location.By similarity1 Publication

Protein-protein interaction databases

BioGridi204893, 1 interactor
IntActiO55047, 1 interactor
STRINGi10090.ENSMUSP00000102554

Structurei

3D structure databases

ProteinModelPortaliO55047
SMRiO55047
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini408 – 687Protein kinasePROSITE-ProRule annotationAdd BLAST280

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili193 – 244Sequence analysisAdd BLAST52
Coiled coili285 – 315Sequence analysisAdd BLAST31
Coiled coili349 – 397Sequence analysisAdd BLAST49

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1151 Eukaryota
ENOG410Y3FX LUCA
GeneTreeiENSGT00790000123008
HOGENOMiHOG000259522
HOVERGENiHBG007938
InParanoidiO55047
KOiK08864
OMAiRKADHFD
PhylomeDBiO55047

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
IPR027086 TLK2
PANTHERiPTHR22974:SF20 PTHR22974:SF20, 2 hits
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket
Note: Additional isoforms seem to exist.1 Publication

This entry has 3 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 11 Publication (identifier: O55047-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MMEELHSLDP RRQELLEARF TGVGVSKGPL NSESSNQSLC SVGSLSDKEV
60 70 80 90 100
ETPEKKQNDQ RNRKRKAEPY DTSQGKGTPR GHKISDYFER RAEQPLYGLD
110 120 130 140 150
GSAAKEASEE QSALPTLMSV MLAKPRLDTE QLAPRGAGLC FTFVSAQQNS
160 170 180 190 200
PSSTGSGNTE HSCSSQKQIS IQHRQTQSDL TIEKISALEN SKNSDLEKKE
210 220 230 240 250
GRIDDLLRAN CDLRRQIDEQ QKMLEKYKER LNRCVTMSKK LLIEKSKQEK
260 270 280 290 300
MACRDKSMQD RLRLGHFTTV RHGASFTEQW TDGYAFQNLI KQQERINSQR
310 320 330 340 350
EEIERQRKML AKRKPPAMGQ APPATNEQKQ RKSKTNGAEN ETLTLAEYHE
360 370 380 390 400
QEEIFKLRLG HLKKEEAEIQ AELERLERVR NLHIRELKRI HNEDNSQFKD
410 420 430 440 450
HPTLNDRYLL LHLLGRGGFS EVYKAFDLTE QRYVAVKIHQ LNKNWRDEKK
460 470 480 490 500
ENYHKHACRE YRIHKELDHP RIVKLYDYFS LDTDSFCTVL EYCEGNDLDF
510 520 530 540 550
YLKQHKLMSE KEARSIIMQI VNALKYLNEI KPPIIHYDLK PGNILLVNGT
560 570 580 590 600
ACGEIKITDF GLSKIMDDDS YNSVDGMELT SQGAGTYWYL PPECFVVGKE
610 620 630 640 650
PPKISNKVDV WSVGVIFYQC LYGRKPFGHN QSQQDILQEN TILKATEVQF
660 670 680 690 700
PPKPVVTPEA KAFIRRCLAY RKEDRIDVQQ LACDPYLLPH IRKSVSTSSP
710
AGAAIASTSG ASNNSSSN
Length:718
Mass (Da):82,261
Last modified:August 22, 2003 - v2
Checksum:i60009617C162BB05
GO
Isoform 2Curated (identifier: O55047-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     89-89: E → EFAGGSGPGTSPGRSVPPVARSSPQHSLSNPLP
     626-718: PFGHNQSQQD...SGASNNSSSN → VRKKAVEQPG...SGLKKIQTSQ

Note: No experimental confirmation available.Combined sourcesCurated
Show »
Length:697
Mass (Da):79,634
Checksum:iC87D35DC2BEA0437
GO
Isoform 31 Publication (identifier: O55047-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2-117: Missing.

Show »
Length:602
Mass (Da):69,388
Checksum:i7D2AD2A4D0A12C80
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B1ASU9B1ASU9_MOUSE
Serine/threonine-protein kinase tou...
Tlk2
750Annotation score:
B7ZC85B7ZC85_MOUSE
Serine/threonine-protein kinase tou...
Tlk2
633Annotation score:
B7ZC84B7ZC84_MOUSE
Serine/threonine-protein kinase tou...
Tlk2
93Annotation score:
D6RHM1D6RHM1_MOUSE
Serine/threonine-protein kinase tou...
Tlk2
112Annotation score:
F6XSD0F6XSD0_MOUSE
Serine/threonine-protein kinase tou...
Tlk2
234Annotation score:

Sequence cautioni

The sequence AAC02225 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti520I → M in BAB29570 (PubMed:16141072).Curated1
Sequence conflicti525K → T in BAB29570 (PubMed:16141072).Curated1
Sequence conflicti535I → T in BAB29570 (PubMed:16141072).Curated1
Sequence conflicti540K → E in BAB29570 (PubMed:16141072).Curated1
Sequence conflicti543N → D in BAB29570 (PubMed:16141072).Curated1
Sequence conflicti548N → D in BAB29570 (PubMed:16141072).Curated1
Sequence conflicti555I → M in BAB29570 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0505742 – 117Missing in isoform 3. 1 PublicationAdd BLAST116
Alternative sequenceiVSP_05057589E → EFAGGSGPGTSPGRSVPPVA RSSPQHSLSNPLP in isoform 2. 1 Publication1
Alternative sequenceiVSP_050576626 – 718PFGHN…NSSSN → VRKKAVEQPGAIPTTSLGFF FAVNHWNPESSGLKKIQTSQ in isoform 2. 1 PublicationAdd BLAST93

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045252 mRNA Translation: AAC02225.1 Different initiation.
AF045254 mRNA Translation: AAC02227.1
AF045253 mRNA Translation: AAC02226.1
AK014829 mRNA Translation: BAB29570.2
AL645471 Genomic DNA No translation available.
CCDSiCCDS36356.2 [O55047-1]
CCDS79062.1 [O55047-2]
RefSeqiNP_001281260.1, NM_001294331.1 [O55047-1]
NP_001281263.1, NM_001294334.1 [O55047-2]
XP_011247316.1, XM_011249014.2 [O55047-1]
UniGeneiMm.126976

Genome annotation databases

EnsembliENSMUST00000015107; ENSMUSP00000015107; ENSMUSG00000020694 [O55047-1]
ENSMUST00000092537; ENSMUSP00000090198; ENSMUSG00000020694 [O55047-2]
ENSMUST00000106939; ENSMUSP00000102552; ENSMUSG00000020694 [O55047-1]
GeneIDi24086
KEGGimmu:24086
UCSCiuc007lxd.3 mouse [O55047-2]
uc007lxg.3 mouse [O55047-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045252 mRNA Translation: AAC02225.1 Different initiation.
AF045254 mRNA Translation: AAC02227.1
AF045253 mRNA Translation: AAC02226.1
AK014829 mRNA Translation: BAB29570.2
AL645471 Genomic DNA No translation available.
CCDSiCCDS36356.2 [O55047-1]
CCDS79062.1 [O55047-2]
RefSeqiNP_001281260.1, NM_001294331.1 [O55047-1]
NP_001281263.1, NM_001294334.1 [O55047-2]
XP_011247316.1, XM_011249014.2 [O55047-1]
UniGeneiMm.126976

3D structure databases

ProteinModelPortaliO55047
SMRiO55047
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204893, 1 interactor
IntActiO55047, 1 interactor
STRINGi10090.ENSMUSP00000102554

PTM databases

iPTMnetiO55047
PhosphoSitePlusiO55047

Proteomic databases

EPDiO55047
MaxQBiO55047
PaxDbiO55047
PeptideAtlasiO55047
PRIDEiO55047

Protocols and materials databases

DNASUi24086
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000015107; ENSMUSP00000015107; ENSMUSG00000020694 [O55047-1]
ENSMUST00000092537; ENSMUSP00000090198; ENSMUSG00000020694 [O55047-2]
ENSMUST00000106939; ENSMUSP00000102552; ENSMUSG00000020694 [O55047-1]
GeneIDi24086
KEGGimmu:24086
UCSCiuc007lxd.3 mouse [O55047-2]
uc007lxg.3 mouse [O55047-1]

Organism-specific databases

CTDi11011
MGIiMGI:1346023 Tlk2

Phylogenomic databases

eggNOGiKOG1151 Eukaryota
ENOG410Y3FX LUCA
GeneTreeiENSGT00790000123008
HOGENOMiHOG000259522
HOVERGENiHBG007938
InParanoidiO55047
KOiK08864
OMAiRKADHFD
PhylomeDBiO55047

Miscellaneous databases

ChiTaRSiTlk2 mouse
PROiPR:O55047
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020694 Expressed in 287 organ(s), highest expression level in muscle tissue
CleanExiMM_TLK2
ExpressionAtlasiO55047 baseline and differential
GenevisibleiO55047 MM

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
IPR027086 TLK2
PANTHERiPTHR22974:SF20 PTHR22974:SF20, 2 hits
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTLK2_MOUSE
AccessioniPrimary (citable) accession number: O55047
Secondary accession number(s): B1ASU7, B1ASU8, Q9D5Y5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 22, 2003
Last sequence update: August 22, 2003
Last modified: November 7, 2018
This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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