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Protein

Rho guanine nucleotide exchange factor 7

Gene

Arhgef7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions. May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons (By similarity).By similarity

GO - Molecular functioni

  • guanyl-nucleotide exchange factor activity Source: RGD
  • protein kinase binding Source: RGD
  • Rho guanyl-nucleotide exchange factor activity Source: InterPro

GO - Biological processi

  • astrocyte cell migration Source: RGD
  • lamellipodium assembly Source: UniProtKB
  • positive regulation of apoptotic process Source: UniProtKB
  • regulation of Rho protein signal transduction Source: InterPro
  • small GTPase mediated signal transduction Source: RGD

Keywordsi

Molecular functionGuanine-nucleotide releasing factor
Biological processNeurogenesis

Enzyme and pathway databases

ReactomeiR-RNO-182971 EGFR downregulation
R-RNO-193648 NRAGE signals death through JNK
R-RNO-194840 Rho GTPase cycle
R-RNO-3928664 Ephrin signaling
R-RNO-416482 G alpha (12/13) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
Rho guanine nucleotide exchange factor 7
Alternative name(s):
Beta-Pix
PAK-interacting exchange factor beta
Gene namesi
Name:Arhgef7
Synonyms:Pak3bp, Pixb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 16

Organism-specific databases

RGDi620624 Arhgef7

Subcellular locationi

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000809231 – 646Rho guanine nucleotide exchange factor 7Add BLAST646

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7PhosphoserineCombined sources1
Modified residuei71PhosphoserineCombined sources1
Modified residuei79PhosphoserineCombined sources1
Modified residuei340PhosphoserineCombined sources1
Modified residuei516PhosphoserineCombined sources1
Modified residuei560PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on Ser-516 by CaMK1; enhancement of GEF activity and downstream activation of RAC1. Phosphorylated by PTK2/FAK1; this promotes interaction with RAC1 (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO55043
PeptideAtlasiO55043
PRIDEiO55043

PTM databases

iPTMnetiO55043
PhosphoSitePlusiO55043

Expressioni

Gene expression databases

BgeeiENSRNOG00000012934
ExpressionAtlasiO55043 baseline and differential
GenevisibleiO55043 RN

Interactioni

Subunit structurei

Interacts with SCRIB; interaction is direct and may play a role in regulation of apoptosis (By similarity). Interacts with PAK kinases through the SH3 domain. Interacts with GIT1 and probably TGFB1I1. Interacts with ITCH and PARVB. Interacts with FRMPD4 (via N-terminus). Interacts with CaMK1. Interacts with PTK2/FAK1 and RAC1. Interacts with BIN2 (By similarity).By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • guanyl-nucleotide exchange factor activity Source: RGD
  • protein kinase binding Source: RGD
  • Rho guanyl-nucleotide exchange factor activity Source: InterPro

Protein-protein interaction databases

BioGridi250377, 1 interactor
DIPiDIP-41481N
ELMiO55043
IntActiO55043, 9 interactors
MINTiO55043
STRINGi10116.ENSRNOP00000043761

Structurei

Secondary structure

1646
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 15Combined sources6
Beta strandi32 – 38Combined sources7
Beta strandi42 – 48Combined sources7
Beta strandi51 – 56Combined sources6
Helixi57 – 59Combined sources3
Beta strandi60 – 62Combined sources3
Helixi588 – 636Combined sources49
Beta strandi640 – 645Combined sources6

3D structure databases

ProteinModelPortaliO55043
SMRiO55043
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO55043

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 65SH3PROSITE-ProRule annotationAdd BLAST60
Domaini93 – 273DHPROSITE-ProRule annotationAdd BLAST181
Domaini295 – 400PHPROSITE-ProRule annotationAdd BLAST106

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG2070 Eukaryota
ENOG410XNNP LUCA
GeneTreeiENSGT00920000148946
HOGENOMiHOG000231381
HOVERGENiHBG050569
InParanoidiO55043
KOiK13710
PhylomeDBiO55043

Family and domain databases

CDDicd00160 RhoGEF, 1 hit
cd12061 SH3_betaPIX, 1 hit
Gene3Di1.20.900.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR035789 BetaPIX_SH3
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR001331 GDS_CDC24_CS
IPR032409 GEF_CC
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PfamiView protein in Pfam
PF16523 betaPIX_CC, 1 hit
PF00169 PH, 1 hit
PF00621 RhoGEF, 1 hit
PF07653 SH3_2, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00325 RhoGEF, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF48065 SSF48065, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS00741 DH_1, 1 hit
PS50010 DH_2, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS50002 SH3, 1 hit

Sequencei

Sequence statusi: Complete.

O55043-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDNANSQLV VRAKFNFQQT NEDELSFSKG DVIHVTRVEE GGWWEGTHNG
60 70 80 90 100
RTGWFPSNYV REIKPSEKPV SPKSGTLKSP PKGFDTTAIN KSYYNVVLQN
110 120 130 140 150
ILETEHEYSK ELQSVLSTYL WPLQTSEKLS SANTSYLMGN LEEISSFQQV
160 170 180 190 200
LVQSLEECTK SPEAQQRVGG CFLSLMPQMR TLYLAYCANH PSAVSVLTEH
210 220 230 240 250
SEDLGEFMET KGASSPGILV LTTGLSKPFM RLDKYPTLLK ELERHMEDYH
260 270 280 290 300
PDRQDIQKSM TAFKNLSAQC QEVRKRKELE LQILTEPIRS WEGDDIKTLG
310 320 330 340 350
SVTYMSQVTI QCAGSEEKNE RYLLLFPNLL LMLSASPRMS GFIYQGKLPT
360 370 380 390 400
TGMTITKLED SENHRNAFEI SGSMIERILV SCNNQQDLHE WVEHLQRQTK
410 420 430 440 450
VTSVSNPTIK PHSVPSHTLP SHPLTPSSKH ADSKPVALTP AYHTLPHPSH
460 470 480 490 500
HGTPHTTISW GPLEPPKTPK PWSLSCLRPA PPLRPSAALC YKEDLSRSPK
510 520 530 540 550
TMKKLLPKRK PERKPSDEEF AVRKSTAALE EDAQILKVIE AYCTSAKTRQ
560 570 580 590 600
TLNSSSRKES APQVLLPEEE KIIVEETKSN GQTVIEEKSL VDTVYALKDE
610 620 630 640
VQELRQDNKK MKKSLEEEQR ARKDLEKLVR KVLKNMNDPA WDETNL
Length:646
Mass (Da):73,140
Last modified:June 1, 1998 - v1
Checksum:i17CDE7DD96ADEA53
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044673 mRNA Translation: AAC39971.1
RefSeqiNP_001106994.1, NM_001113522.1
UniGeneiRn.146763

Genome annotation databases

EnsembliENSRNOT00000017947; ENSRNOP00000017947; ENSRNOG00000012934
GeneIDi114559
KEGGirno:114559
UCSCiRGD:620624 rat

Similar proteinsi

Entry informationi

Entry nameiARHG7_RAT
AccessioniPrimary (citable) accession number: O55043
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 1998
Last modified: July 18, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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