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Protein

Cytoplasmic protein NCK2

Gene

Nck2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTranslation regulation

Enzyme and pathway databases

ReactomeiR-MMU-186763 Downstream signal transduction
R-MMU-373753 Nephrin family interactions
R-MMU-3928664 Ephrin signaling
R-MMU-4420097 VEGFA-VEGFR2 Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic protein NCK2
Alternative name(s):
Growth factor receptor-bound protein 4
NCK adaptor protein 2
Short name:
Nck-2
SH2/SH3 adaptor protein NCK-beta
Gene namesi
Name:Nck2
Synonyms:Grb4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1306821 Nck2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004152872 – 380Cytoplasmic protein NCK2Add BLAST379

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineBy similarity1
Modified residuei90PhosphoserineCombined sources1
Modified residuei92PhosphothreonineBy similarity1
Modified residuei94PhosphoserineCombined sources1
Modified residuei110PhosphotyrosineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO55033
MaxQBiO55033
PaxDbiO55033
PeptideAtlasiO55033
PRIDEiO55033

PTM databases

iPTMnetiO55033
PhosphoSitePlusiO55033

Expressioni

Gene expression databases

BgeeiENSMUSG00000066877 Expressed in 145 organ(s), highest expression level in thymus
ExpressionAtlasiO55033 baseline and differential
GenevisibleiO55033 MM

Interactioni

Subunit structurei

Interacts with DOCK1, LIMS1 and TGFB1I1. Part of a complex containing PPP1R15B, PP1 and NCK2. Interacts with FASLG. Interacts with AXL. Interacts with PAK1, PKN2 and SOS1. Interacts (via SH2 domain) with EGFR. Interacts (via SH2 domain) with DDR1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201705, 8 interactors
IntActiO55033, 6 interactors
MINTiO55033
STRINGi10090.ENSMUSP00000083611

Structurei

3D structure databases

ProteinModelPortaliO55033
SMRiO55033
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 61SH3 1PROSITE-ProRule annotationAdd BLAST60
Domaini111 – 170SH3 2PROSITE-ProRule annotationAdd BLAST60
Domaini195 – 257SH3 3PROSITE-ProRule annotationAdd BLAST63
Domaini285 – 379SH2PROSITE-ProRule annotationAdd BLAST95

Keywords - Domaini

Repeat, SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG4226 Eukaryota
ENOG410XRPF LUCA
GeneTreeiENSGT00820000126999
HOGENOMiHOG000290684
HOVERGENiHBG000719
InParanoidiO55033
KOiK19862
OMAiHSMDELV
OrthoDBiEOG091G0AD0
PhylomeDBiO55033
TreeFamiTF351631

Family and domain databases

CDDicd10409 SH2_Nck2, 1 hit
cd11899 SH3_Nck2_1, 1 hit
cd11902 SH3_Nck2_2, 1 hit
cd11903 SH3_Nck2_3, 1 hit
Gene3Di3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR017304 NCK
IPR035883 Nck2_SH2
IPR035559 Nck2_SH3_1
IPR035560 Nck2_SH3_2
IPR035561 Nck2_SH3_3
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PfamiView protein in Pfam
PF00017 SH2, 1 hit
PF00018 SH3_1, 2 hits
PF14604 SH3_9, 1 hit
PIRSFiPIRSF037874 Cytoplasmic_NCK, 1 hit
PRINTSiPR00401 SH2DOMAIN
PR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00252 SH2, 1 hit
SM00326 SH3, 3 hits
SUPFAMiSSF50044 SSF50044, 3 hits
SSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50001 SH2, 1 hit
PS50002 SH3, 3 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

O55033-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTEEVIVIAK WDYTAQQDQE LDIRKNERLW LLDDSKTWWR VRNAANRTGY
60 70 80 90 100
VPSNYVERKN SLKKGSLVKN LKDTLGLGKT RRKPSARDAS PTPSTDAEYP
110 120 130 140 150
ANGSGADRIY DLNIPAFVKF AYVAEREDEL SLVKGSRVTV MEKCSDGWWR
160 170 180 190 200
GSFNGQIGWF PSNYVLEEAD EAAAEAPSFL SLRRGTALSN GQGARVLHVV
210 220 230 240 250
QTLYPFSSVT EEELSFEKGE TMEVIEKPEN DPEWWKCKNA RGQVGLVPKN
260 270 280 290 300
YVVVLSDGPA LHPAHTPQIS YTGPSASGRF AGREWYYGNV TRHQAECALN
310 320 330 340 350
ERGVEGDFLI RDSESSPSDF SVSLKASGRN KHFKVQLVDS VYCIGQRRFH
360 370 380
SMDELVEHYK KAPIFTSEHG EKLYLVRALQ
Length:380
Mass (Da):42,879
Last modified:June 1, 1998 - v1
Checksum:i7B3404B976F0A54D
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0J9YUK4A0A0J9YUK4_MOUSE
Cytoplasmic protein NCK2
Nck2
142Annotation score:
A0A0A0MQF0A0A0A0MQF0_MOUSE
Cytoplasmic protein NCK2
Nck2
77Annotation score:
A0A087WQT7A0A087WQT7_MOUSE
Cytoplasmic protein NCK2
Nck2
21Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043260 mRNA Translation: AAC06353.1
AC107865 Genomic DNA No translation available.
AC108914 Genomic DNA No translation available.
CH466589 Genomic DNA Translation: EDK96908.1
BC011071 mRNA Translation: AAH11071.1
BC034255 mRNA Translation: AAH34255.1
CCDSiCCDS14924.1
RefSeqiNP_035009.3, NM_010879.3
UniGeneiMm.389903
Mm.483157

Genome annotation databases

EnsembliENSMUST00000086421; ENSMUSP00000083611; ENSMUSG00000066877
GeneIDi17974
KEGGimmu:17974
UCSCiuc007avo.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043260 mRNA Translation: AAC06353.1
AC107865 Genomic DNA No translation available.
AC108914 Genomic DNA No translation available.
CH466589 Genomic DNA Translation: EDK96908.1
BC011071 mRNA Translation: AAH11071.1
BC034255 mRNA Translation: AAH34255.1
CCDSiCCDS14924.1
RefSeqiNP_035009.3, NM_010879.3
UniGeneiMm.389903
Mm.483157

3D structure databases

ProteinModelPortaliO55033
SMRiO55033
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201705, 8 interactors
IntActiO55033, 6 interactors
MINTiO55033
STRINGi10090.ENSMUSP00000083611

PTM databases

iPTMnetiO55033
PhosphoSitePlusiO55033

Proteomic databases

EPDiO55033
MaxQBiO55033
PaxDbiO55033
PeptideAtlasiO55033
PRIDEiO55033

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000086421; ENSMUSP00000083611; ENSMUSG00000066877
GeneIDi17974
KEGGimmu:17974
UCSCiuc007avo.2 mouse

Organism-specific databases

CTDi8440
MGIiMGI:1306821 Nck2

Phylogenomic databases

eggNOGiKOG4226 Eukaryota
ENOG410XRPF LUCA
GeneTreeiENSGT00820000126999
HOGENOMiHOG000290684
HOVERGENiHBG000719
InParanoidiO55033
KOiK19862
OMAiHSMDELV
OrthoDBiEOG091G0AD0
PhylomeDBiO55033
TreeFamiTF351631

Enzyme and pathway databases

ReactomeiR-MMU-186763 Downstream signal transduction
R-MMU-373753 Nephrin family interactions
R-MMU-3928664 Ephrin signaling
R-MMU-4420097 VEGFA-VEGFR2 Pathway

Miscellaneous databases

PROiPR:O55033
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000066877 Expressed in 145 organ(s), highest expression level in thymus
ExpressionAtlasiO55033 baseline and differential
GenevisibleiO55033 MM

Family and domain databases

CDDicd10409 SH2_Nck2, 1 hit
cd11899 SH3_Nck2_1, 1 hit
cd11902 SH3_Nck2_2, 1 hit
cd11903 SH3_Nck2_3, 1 hit
Gene3Di3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR017304 NCK
IPR035883 Nck2_SH2
IPR035559 Nck2_SH3_1
IPR035560 Nck2_SH3_2
IPR035561 Nck2_SH3_3
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PfamiView protein in Pfam
PF00017 SH2, 1 hit
PF00018 SH3_1, 2 hits
PF14604 SH3_9, 1 hit
PIRSFiPIRSF037874 Cytoplasmic_NCK, 1 hit
PRINTSiPR00401 SH2DOMAIN
PR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00252 SH2, 1 hit
SM00326 SH3, 3 hits
SUPFAMiSSF50044 SSF50044, 3 hits
SSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50001 SH2, 1 hit
PS50002 SH3, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiNCK2_MOUSE
AccessioniPrimary (citable) accession number: O55033
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: June 1, 1998
Last modified: November 7, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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