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Protein

Coatomer subunit beta'

Gene

Copb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).By similarity
This coatomer complex protein, essential for Golgi budding and vesicular trafficking, is a selective binding protein (RACK) for protein kinase C, epsilon type. It binds to Golgi membranes in a GTP-dependent manner.

GO - Molecular functioni

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: GO_Central
  • intracellular protein transport Source: GO_Central
  • intra-Golgi vesicle-mediated transport Source: MGI
  • retrograde vesicle-mediated transport, Golgi to ER Source: GO_Central
  • toxin transport Source: MGI

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-6807878 COPI-mediated anterograde transport
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic

Names & Taxonomyi

Protein namesi
Recommended name:
Coatomer subunit beta'
Alternative name(s):
Beta'-coat protein
Short name:
Beta'-COP
p102
Gene namesi
Name:Copb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1354962 Copb2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

The knockout of the gene results in early embryonic lethality with no viable embryos recovered at E12.5.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi254R → C: No effect on protein abundance. Mice homozygous for that mutation do not display any developmental abnormality. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000509131 – 905Coatomer subunit beta'Add BLAST905

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei627N6-acetyllysineBy similarity1
Modified residuei859PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO55029
MaxQBiO55029
PaxDbiO55029
PeptideAtlasiO55029
PRIDEiO55029

PTM databases

iPTMnetiO55029
PhosphoSitePlusiO55029
SwissPalmiO55029

Expressioni

Gene expression databases

BgeeiENSMUSG00000032458 Expressed in 313 organ(s), highest expression level in trachea
GenevisibleiO55029 MM

Interactioni

Subunit structurei

Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Probably interacts with PEX11A. Interacts with SCYL1. Interacts with JAGN1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi206128, 5 interactors
CORUMiO55029
IntActiO55029, 7 interactors
MINTiO55029
STRINGi10090.ENSMUSP00000035033

Structurei

3D structure databases

ProteinModelPortaliO55029
SMRiO55029
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati13 – 52WD 1Add BLAST40
Repeati55 – 94WD 2Add BLAST40
Repeati97 – 136WD 3Add BLAST40
Repeati140 – 180WD 4Add BLAST41
Repeati183 – 224WD 5Add BLAST42
Repeati227 – 266WD 6Add BLAST40
Repeati350 – 388WD 7Add BLAST39
Repeati390 – 425WD 8Add BLAST36
Repeati746 – 783WD 9Add BLAST38

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili867 – 891Sequence analysisAdd BLAST25

Sequence similaritiesi

Belongs to the WD repeat COPB2 family.Curated

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0276 Eukaryota
ENOG410XNNY LUCA
GeneTreeiENSGT00900000141083
HOGENOMiHOG000163444
HOVERGENiHBG051076
InParanoidiO55029
KOiK17302
OMAiFYDWENT
OrthoDBiEOG091G0284
PhylomeDBiO55029
TreeFamiTF300688

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR006692 Coatomer_WD-assoc_reg
IPR016453 COPB2
IPR020472 G-protein_beta_WD-40_rep
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF04053 Coatomer_WDAD, 1 hit
PF00400 WD40, 4 hits
PIRSFiPIRSF005567 Coatomer_beta'_subunit, 1 hit
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 6 hits
SUPFAMiSSF50978 SSF50978, 2 hits
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 5 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

O55029-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPLRLDIKRK LTARSDRVKS VDLHPTEPWM LASLYNGSVC VWNHETQTLV
60 70 80 90 100
KTFEVCDLPV RAAKFVARKN WVVTGADDMQ IRVFNYNTLE RVHMFEAHSD
110 120 130 140 150
YIRCIAVHPT QPFILTSSDD MLIKLWDWDK KWSCSQVFEG HTHYVMQIVI
160 170 180 190 200
NPKDNNQFAS ASLDRTIKVW QLGSSSPNFT LEGHEKGVNC IDYYSGGDKP
210 220 230 240 250
YLISGADDRL VKIWDYQNKT CVQTLEGHAQ NVSCASFHPE LPIIITGSED
260 270 280 290 300
GTVRIWHSST YRLESTLNYG MERVWCVASL RGSNNVALGY DEGSIIVKLG
310 320 330 340 350
REEPAMSMDA NGKIIWAKHS EVQQANLKAM GDTEIKDGER LPLAVKDMGS
360 370 380 390 400
CEIYPQTIQH NPNGRFVVVC GDGEYIIYTA MALRNKSFGS AQEFAWAHDS
410 420 430 440 450
SEYAIRESNS IVKIFKNFKE KKSFKPDFGA ESIYGGFLLG VRSVNGLAFY
460 470 480 490 500
DWENTELIRR IEIQPKHIFW SDSGELVCIA TEESFFILKY LSEKVLAAQE
510 520 530 540 550
THEGVTEDGI EDAFEVLGEI QEIVKTGLWV GDCFIYTSSV NRLNYYVGGE
560 570 580 590 600
IVTIAHLDRT MYLLGYIPKD NRLYLGDKEL NIVSYSLLVS VLEYQTAVMR
610 620 630 640 650
RDFSMADKVL PTIPKEQRTR VAHFLEKQGF KQQALTVSTD PEHRFELALQ
660 670 680 690 700
LGELKIAYQL AVEAESEQKW KQLAELAISK CQFSLAQECL HHAQDYGGLL
710 720 730 740 750
LLATASGNAS MVNKLAEGAE RDGKNNVAFM SYFLQGKLDA CLELLIRTGR
760 770 780 790 800
LPEAAFLART YLPSQVSRVV KLWRENLSKV NQKAAESLAD PTEYENLFPG
810 820 830 840 850
LKEAFVVEEW VKETHADLWP AKQYPLVTPN EERNVMEEAK GFQPSRPTAQ
860 870 880 890 900
QEPDGKPASS PVIMASQTTH KEEKSLLELE VDLDNLELED IDTTDINLDE

DILDD
Length:905
Mass (Da):102,449
Last modified:August 29, 2001 - v2
Checksum:i5845082CAB10FD12
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti684S → H in AAB97760 (Ref. 3) Curated1
Sequence conflicti733F → L in AAB97760 (Ref. 3) Curated1
Sequence conflicti839A → P in AAB97760 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK088064 mRNA Translation: BAC40125.1
AK153352 mRNA Translation: BAE31926.1
AF043120 mRNA Translation: AAB97760.1
BC006675 mRNA Translation: AAH06675.1
CCDSiCCDS40734.1
RefSeqiNP_056642.1, NM_015827.2
UniGeneiMm.400464

Genome annotation databases

EnsembliENSMUST00000035033; ENSMUSP00000035033; ENSMUSG00000032458
GeneIDi50797
KEGGimmu:50797
UCSCiuc009rdm.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK088064 mRNA Translation: BAC40125.1
AK153352 mRNA Translation: BAE31926.1
AF043120 mRNA Translation: AAB97760.1
BC006675 mRNA Translation: AAH06675.1
CCDSiCCDS40734.1
RefSeqiNP_056642.1, NM_015827.2
UniGeneiMm.400464

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5A1Uelectron microscopy13.00D1-905[»]
5A1Velectron microscopy21.00D/L/U1-905[»]
5A1Welectron microscopy18.00D1-905[»]
5A1Xelectron microscopy23.00D/L1-905[»]
5A1Yelectron microscopy21.00D/L1-905[»]
5NZRelectron microscopy9.20C1-905[»]
5NZSelectron microscopy10.10C1-905[»]
5NZTelectron microscopy17.00C/H1-905[»]
5NZUelectron microscopy15.00C1-905[»]
5NZVelectron microscopy17.30C/J1-905[»]
ProteinModelPortaliO55029
SMRiO55029
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206128, 5 interactors
CORUMiO55029
IntActiO55029, 7 interactors
MINTiO55029
STRINGi10090.ENSMUSP00000035033

PTM databases

iPTMnetiO55029
PhosphoSitePlusiO55029
SwissPalmiO55029

Proteomic databases

EPDiO55029
MaxQBiO55029
PaxDbiO55029
PeptideAtlasiO55029
PRIDEiO55029

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035033; ENSMUSP00000035033; ENSMUSG00000032458
GeneIDi50797
KEGGimmu:50797
UCSCiuc009rdm.1 mouse

Organism-specific databases

CTDi9276
MGIiMGI:1354962 Copb2

Phylogenomic databases

eggNOGiKOG0276 Eukaryota
ENOG410XNNY LUCA
GeneTreeiENSGT00900000141083
HOGENOMiHOG000163444
HOVERGENiHBG051076
InParanoidiO55029
KOiK17302
OMAiFYDWENT
OrthoDBiEOG091G0284
PhylomeDBiO55029
TreeFamiTF300688

Enzyme and pathway databases

ReactomeiR-MMU-6807878 COPI-mediated anterograde transport
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic

Miscellaneous databases

PROiPR:O55029
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032458 Expressed in 313 organ(s), highest expression level in trachea
GenevisibleiO55029 MM

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR006692 Coatomer_WD-assoc_reg
IPR016453 COPB2
IPR020472 G-protein_beta_WD-40_rep
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF04053 Coatomer_WDAD, 1 hit
PF00400 WD40, 4 hits
PIRSFiPIRSF005567 Coatomer_beta'_subunit, 1 hit
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 6 hits
SUPFAMiSSF50978 SSF50978, 2 hits
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 5 hits
PS50294 WD_REPEATS_REGION, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCOPB2_MOUSE
AccessioniPrimary (citable) accession number: O55029
Secondary accession number(s): Q3U5Z9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: August 29, 2001
Last modified: November 7, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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