UniProtKB - O55023 (IMPA1_MOUSE)
Protein
Inositol monophosphatase 1
Gene
Impa1
Organism
Mus musculus (Mouse)
Status
Functioni
Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides and has been implicated as the pharmacological target for lithium action in brain. Has broad substrate specificity and can use myo-inositol monophosphates, myo-inositol 1,3-diphosphate, myo-inositol 1,4-diphosphate, scyllo-inositol-phosphate, D-galactose 1-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates.By similarity
Catalytic activityi
Cofactori
Mg2+1 Publication
Activity regulationi
Inhibited by Li+, Ca2+ and Mn2+, but also by Mg2+ at concentrations above 3 mM.By similarity
: myo-inositol biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes myo-inositol from D-glucose 6-phosphate.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- Inositol-3-phosphate synthase 1 (Isyna1)
- Inositol monophosphatase 1 (Impa1), Inositol-1-monophosphatase (Impa2), Inositol-1-monophosphatase (Impa1), Inositol monophosphatase 2 (Impa2), Inositol-1-monophosphatase (Impa1), Inositol-1-monophosphatase (Impa1), Inositol-1-monophosphatase (Impa2), Inositol-1-monophosphatase (Impa1), Inositol-1-monophosphatase (Impa2), Inositol-1-monophosphatase (Impa1), Inositol-1-monophosphatase (Impa2), Inositol-1-monophosphatase (Impa1)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes myo-inositol from D-glucose 6-phosphate, the pathway myo-inositol biosynthesis and in Polyol metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 70 | Magnesium 1; catalytic1 Publication | 1 | |
Binding sitei | 70 | SubstrateCurated | 1 | |
Metal bindingi | 90 | Magnesium 1; catalytic1 Publication | 1 | |
Metal bindingi | 90 | Magnesium 21 Publication | 1 | |
Metal bindingi | 92 | Magnesium 1; via carbonyl oxygen; catalytic1 Publication | 1 | |
Metal bindingi | 93 | Magnesium 21 Publication | 1 | |
Binding sitei | 213 | SubstrateBy similarity | 1 | |
Metal bindingi | 220 | Magnesium 21 Publication | 1 | |
Binding sitei | 220 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- identical protein binding Source: MGI
- inositol monophosphate 1-phosphatase activity Source: MGI
- inositol monophosphate 3-phosphatase activity Source: UniProtKB-EC
- inositol monophosphate 4-phosphatase activity Source: UniProtKB-EC
- inositol monophosphate phosphatase activity Source: UniProtKB
- lithium ion binding Source: UniProtKB
- magnesium ion binding Source: UniProtKB
- manganese ion binding Source: UniProtKB
- protein homodimerization activity Source: MGI
GO - Biological processi
- inositol biosynthetic process Source: UniProtKB-UniPathway
- inositol metabolic process Source: GO_Central
- inositol phosphate dephosphorylation Source: UniProtKB
- phosphate-containing compound metabolic process Source: MGI
- phosphatidylinositol biosynthetic process Source: MGI
- phosphatidylinositol phosphorylation Source: InterPro
- signal transduction Source: MGI
Keywordsi
Molecular function | Hydrolase |
Ligand | Lithium, Magnesium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-1855183, Synthesis of IP2, IP, and Ins in the cytosol |
UniPathwayi | UPA00823;UER00788 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Impa1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1933158, Impa1 |
Subcellular locationi
Other locations
- Cytoplasm By similarity
Other locations
- axon Source: MGI
- cytoplasm Source: MGI
- neuronal cell body Source: MGI
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000142514 | 1 – 277 | Inositol monophosphatase 1Add BLAST | 277 |
Proteomic databases
EPDi | O55023 |
jPOSTi | O55023 |
MaxQBi | O55023 |
PaxDbi | O55023 |
PeptideAtlasi | O55023 |
PRIDEi | O55023 |
PTM databases
iPTMneti | O55023 |
PhosphoSitePlusi | O55023 |
SwissPalmi | O55023 |
Expressioni
Tissue specificityi
Mostly expressed in brain, small intestine, testis, kidney, and spleen (at protein level).1 Publication
Inductioni
By lithium Li+ in hippocamp.1 Publication
Interactioni
Subunit structurei
Homodimer.
1 PublicationGO - Molecular functioni
- identical protein binding Source: MGI
- protein homodimerization activity Source: MGI
Protein-protein interaction databases
IntActi | O55023, 2 interactors |
STRINGi | 10090.ENSMUSP00000068174 |
Miscellaneous databases
RNActi | O55023, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O55023 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 90 – 95 | Substrate bindingCurated | 6 | |
Regioni | 194 – 196 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the inositol monophosphatase superfamily.Curated
Phylogenomic databases
eggNOGi | KOG2951, Eukaryota |
InParanoidi | O55023 |
PhylomeDBi | O55023 |
Family and domain databases
CDDi | cd01639, IMPase, 1 hit |
InterProi | View protein in InterPro IPR033942, IMPase IPR020583, Inositol_monoP_metal-BS IPR020552, Inositol_monoPase_Li-sen IPR000760, Inositol_monophosphatase-like IPR020550, Inositol_monophosphatase_CS |
Pfami | View protein in Pfam PF00459, Inositol_P, 1 hit |
PRINTSi | PR00377, IMPHPHTASES PR00378, LIIMPHPHTASE |
PROSITEi | View protein in PROSITE PS00629, IMP_1, 1 hit PS00630, IMP_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
O55023-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MADPWQECMD YAVILARQAG EMIREALKNE MDVMIKSSPA DLVTVTDQKV
60 70 80 90 100
EKMLMSSIKE KYPCHSFIGE ESVAAGEKTV FTESPTWFID PIDGTTNFVH
110 120 130 140 150
RFPFVAVSIG FLVNKEMEFG IVYSCVEDKM YTGRKGKGAF CNGQKLQVSQ
160 170 180 190 200
QEDITKSLLV TELGSSRKPE TLRIVLSNME KLCSIPIHGI RSVGTAAVNM
210 220 230 240 250
CLVATGGADA YYEMGIHCWD MAGAGIIVTE AGGVLMDVTG GPFDLMSRRI
260 270
IAANSITLAK RIAKEIEIIP LQRDDES
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ924B0 | Q924B0_MOUSE | Inositol-1-monophosphatase | Impa1 | 277 | Annotation score: | ||
A0A0A6YW07 | A0A0A6YW07_MOUSE | Inositol-1-monophosphatase | Impa1 | 208 | Annotation score: | ||
Q80ZJ2 | Q80ZJ2_MOUSE | Inositol-1-monophosphatase | Impa1 | 276 | Annotation score: | ||
D3Z703 | D3Z703_MOUSE | Inositol monophosphatase 1 | Impa1 | 89 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF042730 mRNA Translation: AAB97469.1 |
CCDSi | CCDS17240.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF042730 mRNA Translation: AAB97469.1 |
CCDSi | CCDS17240.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4AS5 | X-ray | 2.43 | A/B/C/D | 1-277 | [»] | |
SMRi | O55023 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | O55023, 2 interactors |
STRINGi | 10090.ENSMUSP00000068174 |
PTM databases
iPTMneti | O55023 |
PhosphoSitePlusi | O55023 |
SwissPalmi | O55023 |
Proteomic databases
EPDi | O55023 |
jPOSTi | O55023 |
MaxQBi | O55023 |
PaxDbi | O55023 |
PeptideAtlasi | O55023 |
PRIDEi | O55023 |
Organism-specific databases
MGIi | MGI:1933158, Impa1 |
Phylogenomic databases
eggNOGi | KOG2951, Eukaryota |
InParanoidi | O55023 |
PhylomeDBi | O55023 |
Enzyme and pathway databases
UniPathwayi | UPA00823;UER00788 |
Reactomei | R-MMU-1855183, Synthesis of IP2, IP, and Ins in the cytosol |
Miscellaneous databases
ChiTaRSi | Impa1, mouse |
PROi | PR:O55023 |
RNActi | O55023, protein |
SOURCEi | Search... |
Family and domain databases
CDDi | cd01639, IMPase, 1 hit |
InterProi | View protein in InterPro IPR033942, IMPase IPR020583, Inositol_monoP_metal-BS IPR020552, Inositol_monoPase_Li-sen IPR000760, Inositol_monophosphatase-like IPR020550, Inositol_monophosphatase_CS |
Pfami | View protein in Pfam PF00459, Inositol_P, 1 hit |
PRINTSi | PR00377, IMPHPHTASES PR00378, LIIMPHPHTASE |
PROSITEi | View protein in PROSITE PS00629, IMP_1, 1 hit PS00630, IMP_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IMPA1_MOUSE | |
Accessioni | O55023Primary (citable) accession number: O55023 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | June 1, 1998 | |
Last modified: | August 12, 2020 | |
This is version 144 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families