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Protein

Contactin-associated protein 1

Gene

Cntnap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required, with CNTNAP2, for radial and longitudinal organization of myelinated axons (PubMed:25378149). Plays a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Demarcates the paranodal region of the axo-glial junction. In association with contactin involved in the signaling between axons and myelinating glial cells (PubMed:25378149, PubMed:11395000).2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Contactin-associated protein 1
Short name:
Caspr
Short name:
Caspr1
Alternative name(s):
MHDNIV
NCP1
Neurexin IV
Neurexin-4
Paranodin
Gene namesi
Name:Cntnap1
Synonyms:Nrxn4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1858201 Cntnap1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 1284ExtracellularSequence analysisAdd BLAST1264
Transmembranei1285 – 1305HelicalSequence analysisAdd BLAST21
Topological domaini1306 – 1385CytoplasmicSequence analysisAdd BLAST80

Keywords - Cellular componenti

Cell junction, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant mice exhibit tremor, ataxia, and significant motor paresis. Normal paranodal junctions fail to form, and the organization of the paranodal loops is disrupted. Contactin is undetectable in the paranodes, and potassium channels are displaced from the juxtaparanodal into the paranodal domains. Also results in a severe decrease in peripheral nerve conduction velocity (PubMed:11395000, PubMed:25378149). Double mutants CNTNAP1 and CNTNAP2 have wider Ranvier nodes compared to wild-type littermates (PubMed:25378149).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000001950421 – 1385Contactin-associated protein 1Add BLAST1365

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 169By similarity
Glycosylationi121N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi129N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi277N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi324 ↔ 356By similarity
Glycosylationi421N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi500N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi507 ↔ 539By similarity
Glycosylationi519N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi545 ↔ 556By similarity
Disulfide bondi550 ↔ 565By similarity
Disulfide bondi567 ↔ 577By similarity
Glycosylationi598N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi654N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi665N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi764N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi805N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi844N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi861N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi931 ↔ 958By similarity
Glycosylationi949N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi957N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi962 ↔ 975By similarity
Disulfide bondi969 ↔ 984By similarity
Disulfide bondi986 ↔ 996By similarity
Glycosylationi1079N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1148N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1210 ↔ 1251By similarity
Modified residuei1384PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiO54991
PaxDbiO54991
PeptideAtlasiO54991
PRIDEiO54991

PTM databases

iPTMnetiO54991
PhosphoSitePlusiO54991
SwissPalmiO54991

Expressioni

Tissue specificityi

Expressed in brain. In myelinated nerve fibers predominantly found in paranodal axoglial junctions. In the internodal region of myelinated axons in the CNS and the PNS also found as a thin line apposing the inner mesaxon of the myelin sheath. In PNS neurons this line forms a circumferential ring that apposes the innermost aspect of Schmidt-Lanterman incisures.2 Publications

Gene expression databases

BgeeiENSMUSG00000017167 Expressed in 66 organ(s), highest expression level in cerebellum
CleanExiMM_CNTNAP1
GenevisibleiO54991 MM

Interactioni

Subunit structurei

Interacts with CNTN1/contactin in cis form.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207284, 6 interactors
IntActiO54991, 8 interactors
MINTiO54991
STRINGi10090.ENSMUSP00000099398

Structurei

3D structure databases

ProteinModelPortaliO54991
SMRiO54991
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 169F5/8 type CPROSITE-ProRule annotationAdd BLAST144
Domaini204 – 356Laminin G-like 1PROSITE-ProRule annotationAdd BLAST153
Domaini390 – 539Laminin G-like 2PROSITE-ProRule annotationAdd BLAST150
Domaini545 – 577EGF-like 1PROSITE-ProRule annotationAdd BLAST33
Domaini577 – 796Fibrinogen C-terminalPROSITE-ProRule annotationAdd BLAST220
Domaini814 – 958Laminin G-like 3PROSITE-ProRule annotationAdd BLAST145
Domaini962 – 996EGF-like 2PROSITE-ProRule annotationAdd BLAST35
Domaini1089 – 1251Laminin G-like 4PROSITE-ProRule annotationAdd BLAST163

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1334 – 1370SH3-bindingSequence analysisAdd BLAST37

Sequence similaritiesi

Belongs to the neurexin family.Curated

Keywords - Domaini

EGF-like domain, Repeat, SH3-binding, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3516 Eukaryota
ENOG410XPHG LUCA
GeneTreeiENSGT00760000118991
HOGENOMiHOG000230964
HOVERGENiHBG057718
InParanoidiO54991
KOiK07379
OMAiRHDLHYH
OrthoDBiEOG091G00LF
TreeFamiTF321823

Family and domain databases

CDDicd00057 FA58C, 1 hit
Gene3Di2.60.120.260, 1 hit
InterProiView protein in InterPro
IPR028872 Caspr1
IPR013320 ConA-like_dom_sf
IPR000742 EGF-like_dom
IPR000421 FA58C
IPR036056 Fibrinogen-like_C
IPR002181 Fibrinogen_a/b/g_C_dom
IPR008979 Galactose-bd-like_sf
IPR001791 Laminin_G
IPR003585 Neurexin-like
PANTHERiPTHR43925:SF5 PTHR43925:SF5, 1 hit
PfamiView protein in Pfam
PF00754 F5_F8_type_C, 1 hit
PF02210 Laminin_G_2, 4 hits
SMARTiView protein in SMART
SM00294 4.1m, 1 hit
SM00231 FA58C, 1 hit
SM00282 LamG, 4 hits
SUPFAMiSSF49785 SSF49785, 1 hit
SSF49899 SSF49899, 4 hits
SSF56496 SSF56496, 1 hit
PROSITEiView protein in PROSITE
PS50026 EGF_3, 2 hits
PS01285 FA58C_1, 1 hit
PS01286 FA58C_2, 1 hit
PS50022 FA58C_3, 1 hit
PS51406 FIBRINOGEN_C_2, 1 hit
PS50025 LAM_G_DOMAIN, 4 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O54991-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MMSLRLFSIL LATVVSGAWG WGYYGCNEEL VGPLYARSLG ASSYYGLFTT
60 70 80 90 100
ARFARLHGIS GWSPRIGDPN PWLQIDLMKK HRIRAVATQG AFNSWDWVTR
110 120 130 140 150
YMLLYGDRVD SWTPFYQKGH NATFFGNVND SAVVRHDLHY HFTARYIRIV
160 170 180 190 200
PLAWNPRGKI GLRLGIYGCP YTSSILYFDG DDAISYRFQR GASQSLWDVF
210 220 230 240 250
AFSFKTEEKD GLLLHTEGSQ GDYVTLELQG AHLLLHMSLG SSPIQPRPGH
260 270 280 290 300
TTVSLGGVLN DLSWHYVRVD RYGRDANFTL DGYAHHFVLN GDFERLNLEN
310 320 330 340 350
EIFIGGLVGA ARKNLAYRHN FRGCIENVIY NRINIAEMAV MRHSRITFEG
360 370 380 390 400
NVAFRCLDPV PHPINFGGPH NFVQVPGFPR RGRLAVSFRF RTWDLTGLLL
410 420 430 440 450
FSHLGDGLGH VELMLSEGQV NVSIAQTGRK KLQFAAGYRL NDGFWHEVNF
460 470 480 490 500
VAQENHAVIS IDDVEGAEVR VSYPLLIRTG TSYFFGGCPK PASRWGCHSN
510 520 530 540 550
QTAFHGCMEL LKVDGQLVNL TLVEFRKLGY FAEVLFDTCG ITDRCSPNMC
560 570 580 590 600
EHDGRCYQSW DDFICYCELT GYKGVTCHEP LYKESCEAYR LSGKYSGNYT
610 620 630 640 650
IDPDGSGPLK PFVVYCDIRE NRAWTVVRHD RLWTTRVTGS SMDRPFLGAI
660 670 680 690 700
QYWNASWEEV SALANASQHC EQWIEFSCYN SRLLNTAGGY PYSFWIGRNE
710 720 730 740 750
EQHFYWGGSQ PGIQRCACGL DQSCVDPALH CNCDADQPQW RTDKGLLTFV
760 770 780 790 800
DHLPVTQVVV GDTNRSNSEA QFFLRPLRCY GDRNSWNTIS FHTGAALRFP
810 820 830 840 850
PIRANHSLDV SFYFRTSAPS GVFLENMGGP FCRWRRPYVR VELNTSRDVV
860 870 880 890 900
FAFDIGNGDE NLTVHSDDFE FNDDEWHLVR AEINVKQARL RVDHRPWVLR
910 920 930 940 950
PMPLQTYIWL VYDQPLYVGS AELKRRPFVG CLRAMRLNGV TLNLEGRANA
960 970 980 990 1000
SEGTFPNCTG HCTHPRFPCF HGGRCVERYS YYTCDCDLTA FDGPYCNHDI
1010 1020 1030 1040 1050
GGFFETGTWM RYNLQSALRS AAREFSHMLS RPVPGYEPGY VPGYDTPGYV
1060 1070 1080 1090 1100
PGYHGPGYRL PEYPRPGRPV PGYRGPVYNV TGEEVSFSFS TNSAPAVLLY
1110 1120 1130 1140 1150
VSSFVRDYMA VLIKEDGTLQ LRYQLGTSPY VYQLTTRPVT DGQPHSVNIT
1160 1170 1180 1190 1200
RVYRNLFIQV DYFPLTEQKF SLLVDSQLDS PKALYLGRVM ETGVIDPEIQ
1210 1220 1230 1240 1250
RYNTPGFSGC LSGVRFNNVA PLKTHFRTPR PMTAELAEAM RVQGELSESN
1260 1270 1280 1290 1300
CGAMPRLVSE VPPELDPWYL PPDFPYYHDD GWIAILLGFL VAFLLLGLVG
1310 1320 1330 1340 1350
MLVLFYLQNH RYKGSYHTNE PKATHDSHPG GKAPLPPSGP AQAPAPTPAP
1360 1370 1380
TQLPTPAPAP APAPASGPGP RDQNLPQILE ESRSE
Length:1,385
Mass (Da):156,312
Last modified:July 27, 2011 - v2
Checksum:iB10BA6F150C27BA2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti689G → S in AAB96760 (PubMed:11395000).Curated1
Sequence conflicti1134 – 1136LTT → VTR in AAB96760 (PubMed:11395000).Curated3
Sequence conflicti1262P → Q in AAB96760 (PubMed:11395000).Curated1
Sequence conflicti1313K → Q in AAB96760 (PubMed:11395000).Curated1
Sequence conflicti1326D → H in AAB96760 (PubMed:11395000).Curated1
Sequence conflicti1329P → S in AAB96760 (PubMed:11395000).Curated1
Sequence conflicti1347T → I in AAB96760 (PubMed:11395000).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039833 mRNA Translation: AAB96760.1
AL590969 Genomic DNA No translation available.
CCDSiCCDS25455.1
PIRiT14158
RefSeqiNP_058062.2, NM_016782.2
UniGeneiMm.474527

Genome annotation databases

EnsembliENSMUST00000103109; ENSMUSP00000099398; ENSMUSG00000017167
GeneIDi53321
KEGGimmu:53321
UCSCiuc007lnt.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039833 mRNA Translation: AAB96760.1
AL590969 Genomic DNA No translation available.
CCDSiCCDS25455.1
PIRiT14158
RefSeqiNP_058062.2, NM_016782.2
UniGeneiMm.474527

3D structure databases

ProteinModelPortaliO54991
SMRiO54991
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207284, 6 interactors
IntActiO54991, 8 interactors
MINTiO54991
STRINGi10090.ENSMUSP00000099398

PTM databases

iPTMnetiO54991
PhosphoSitePlusiO54991
SwissPalmiO54991

Proteomic databases

MaxQBiO54991
PaxDbiO54991
PeptideAtlasiO54991
PRIDEiO54991

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103109; ENSMUSP00000099398; ENSMUSG00000017167
GeneIDi53321
KEGGimmu:53321
UCSCiuc007lnt.1 mouse

Organism-specific databases

CTDi8506
MGIiMGI:1858201 Cntnap1

Phylogenomic databases

eggNOGiKOG3516 Eukaryota
ENOG410XPHG LUCA
GeneTreeiENSGT00760000118991
HOGENOMiHOG000230964
HOVERGENiHBG057718
InParanoidiO54991
KOiK07379
OMAiRHDLHYH
OrthoDBiEOG091G00LF
TreeFamiTF321823

Miscellaneous databases

PROiPR:O54991
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000017167 Expressed in 66 organ(s), highest expression level in cerebellum
CleanExiMM_CNTNAP1
GenevisibleiO54991 MM

Family and domain databases

CDDicd00057 FA58C, 1 hit
Gene3Di2.60.120.260, 1 hit
InterProiView protein in InterPro
IPR028872 Caspr1
IPR013320 ConA-like_dom_sf
IPR000742 EGF-like_dom
IPR000421 FA58C
IPR036056 Fibrinogen-like_C
IPR002181 Fibrinogen_a/b/g_C_dom
IPR008979 Galactose-bd-like_sf
IPR001791 Laminin_G
IPR003585 Neurexin-like
PANTHERiPTHR43925:SF5 PTHR43925:SF5, 1 hit
PfamiView protein in Pfam
PF00754 F5_F8_type_C, 1 hit
PF02210 Laminin_G_2, 4 hits
SMARTiView protein in SMART
SM00294 4.1m, 1 hit
SM00231 FA58C, 1 hit
SM00282 LamG, 4 hits
SUPFAMiSSF49785 SSF49785, 1 hit
SSF49899 SSF49899, 4 hits
SSF56496 SSF56496, 1 hit
PROSITEiView protein in PROSITE
PS50026 EGF_3, 2 hits
PS01285 FA58C_1, 1 hit
PS01286 FA58C_2, 1 hit
PS50022 FA58C_3, 1 hit
PS51406 FIBRINOGEN_C_2, 1 hit
PS50025 LAM_G_DOMAIN, 4 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCNTP1_MOUSE
AccessioniPrimary (citable) accession number: O54991
Secondary accession number(s): A2A4K6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 170 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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