Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Prominin-1

Gene

Prom1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis (PubMed:19228982). Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.1 Publication

Miscellaneous

Fundus images and light microscopy of retinal sections from transgenic mice expressing mutant PROM1 reveal progressive retinal abnormalities visible as subretinal deposits and photoreceptor atrophy.

GO - Molecular functioni

  • actinin binding Source: MGI
  • cadherin binding Source: MGI
  • cholesterol binding Source: GO_Central

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Prominin-1
Alternative name(s):
Antigen AC133 homolog
Prominin-like protein 1
CD_antigen: CD133
Gene namesi
Name:Prom1
Synonyms:Prom, Proml1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1100886 Prom1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 107ExtracellularSequence analysisAdd BLAST88
Transmembranei108 – 128HelicalSequence analysisAdd BLAST21
Topological domaini129 – 158CytoplasmicSequence analysisAdd BLAST30
Transmembranei159 – 179HelicalSequence analysisAdd BLAST21
Topological domaini180 – 434ExtracellularSequence analysisAdd BLAST255
Transmembranei435 – 455HelicalSequence analysisAdd BLAST21
Topological domaini456 – 487CytoplasmicSequence analysisAdd BLAST32
Transmembranei488 – 508HelicalSequence analysisAdd BLAST21
Topological domaini509 – 794ExtracellularSequence analysisAdd BLAST286
Transmembranei795 – 815HelicalSequence analysisAdd BLAST21
Topological domaini816 – 867CytoplasmicSequence analysisAdd BLAST52

Keywords - Cellular componenti

Cell membrane, Cell projection, Cilium, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Progressive degeneration and functional deterioration of both cone and rod photoreceptors associated with impaired morphogenesis of the disks and OS.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000002581420 – 867Prominin-1Add BLAST848

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei226N6-acetyllysineBy similarity1
Modified residuei258N6-acetyllysineBy similarity1
Modified residuei265N6-acetyllysineBy similarity1
Glycosylationi273N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi291N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi332N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi374N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi415N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi554N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi581N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi732N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei865PhosphoserineBy similarity1

Post-translational modificationi

Acetylation at Lys-226, Lys-258 and Lys-265 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiO54990
PaxDbiO54990
PeptideAtlasiO54990
PRIDEiO54990

PTM databases

iPTMnetiO54990
PhosphoSitePlusiO54990

Expressioni

Tissue specificityi

In the submandibular gland, expressed on the apical side of epithelial cells. In the parotid gland, expressed in the intercalated ducts. In the sublingual gland, expressed in intercalated ducts. In the extraorbital lacrimal gland, expressed in the intercalated tubules and larger intralobular ducts. Expressed in the retina. Present in urine within small membrane particles (at protein level). In the embryo, expressed on the apical side of neuroepithelial cells and of other epithelia such as lung buds, gut and ureter buds. In the adult, expressed at the apical side of the kidney tubules and of the ependymal layer of the brain. Not expressed in gut, liver, lung, pituitary, adrenal, heart or spleen. Localized to the nascent disk membranes at the base of the rod outer segment in the retina (at protein level).4 Publications

Developmental stagei

At birth, is detected at the interface between the developing neuroretina and the retinal pigment epithelium (RPE) layer. In the postnatal retina (P20) detected in photoreceptors, with particular concentration in the region of plasma membrane evaginations at the basal part of the outer segment (OS). Expressed by rod and cone photoreceptor cells, most abundantly between the OS and inner segment (IS), in close proximity to the connecting cilium.1 Publication

Gene expression databases

CleanExiMM_PROM1

Interactioni

Subunit structurei

Interacts with CDHR1 and with actin filaments. Interacts with NAT8 and NAT8B (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202393, 1 interactor
IntActiO54990, 1 interactor
MINTiO54990
STRINGi10090.ENSMUSP00000073751

Structurei

3D structure databases

ProteinModelPortaliO54990
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the prominin family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4331 Eukaryota
ENOG410XRG0 LUCA
HOGENOMiHOG000115704
HOVERGENiHBG053690
InParanoidiO54990
KOiK06532
PhylomeDBiO54990

Family and domain databases

InterProiView protein in InterPro
IPR008795 Prominin
PANTHERiPTHR22730 PTHR22730, 1 hit
PfamiView protein in Pfam
PF05478 Prominin, 1 hit

Sequences (6+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket
Note: Additional isoforms seem to exist.

This entry has 6 described isoforms and 10 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O54990-1) [UniParc]FASTAAdd to basket
Also known as: S2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MALVFSALLL LGLCGKISSE GQPAFHNTPG AMNYELPTTK YETQDTFNAG
60 70 80 90 100
IVGPLYKMVH IFLSVVQPND FPLDLIKKLI QNKKFDISVD SKEPEIIVLA
110 120 130 140 150
LKIALYEIGV LICAILGLLF IILMPLVGCF FCMCRCCNKC GGEMHQRQKQ
160 170 180 190 200
NAPCRRKCLG LSLLVICLLM SLGIIYGFVA NQQTRTRIKG TQKLAKSNFR
210 220 230 240 250
DFQTLLTETP KQIDYVVEQY TNTKNKAFSD LDGIGSVLGG RIKDQLKPKV
260 270 280 290 300
TPVLEEIKAM ATAIKQTKDA LQNMSSSLKS LQDAATQLNT NLSSVRNSIE
310 320 330 340 350
NSLSSSDCTS DPASKICDSI RPSLSSLGSS LNSSQLPSVD RELNTVTEVD
360 370 380 390 400
KTDLESLVKR GYTTIDEIPN TIQNQTVDVI KDVKNTLDSI SSNIKDMSQS
410 420 430 440 450
IPIEDMLLQV SHYLNNSNRY LNQELPKLEE YDSYWWLGGL IVCFLLTLIV
460 470 480 490 500
TFFFLGLLCG VFGYDKHATP TRRGCVSNTG GIFLMAGVGF GFLFCWILMI
510 520 530 540 550
LVVLTFVVGA NVEKLLCEPY ENKKLLQVLD TPYLLKEQWQ FYLSGMLFNN
560 570 580 590 600
PDINMTFEQV YRDCKRGRGI YAAFQLENVV NVSDHFNIDQ ISENINTELE
610 620 630 640 650
NLNVNIDSIE LLDNTGRKSL EDFAHSGIDT IDYSTYLKET EKSPTEVNLL
660 670 680 690 700
TFASTLEAKA NQLPEGKPKQ AFLLDVQNIR AIHQHLLPPV QQSLNTLRQS
710 720 730 740 750
VWTLQQTSNK LPEKVKKILA SLDSVQHFLT NNVSLIVIGE TKKFGKTILG
760 770 780 790 800
YFEHYLHWVF YAITEKMTSC KPMATAMDSA VNGILCGYVA DPLNLFWFGI
810 820 830 840 850
GKATVLLLPA VIIAIKLAKY YRRMDSEDVY DDVETVPMKN LEIGSNGYHK
860
DHLYGVHNPV MTSPSRY
Length:867
Mass (Da):97,113
Last modified:June 1, 1998 - v1
Checksum:iD442F6372552B3C8
GO
Isoform 2 (identifier: O54990-2) [UniParc]FASTAAdd to basket
Also known as: S1

The sequence of this isoform differs from the canonical sequence as follows:
     94-102: Missing.

Show »
Length:858
Mass (Da):96,136
Checksum:iCAFCAD4C3039848D
GO
Isoform 3 (identifier: O54990-3) [UniParc]FASTAAdd to basket
Also known as: S3

The sequence of this isoform differs from the canonical sequence as follows:
     94-102: Missing.
     833-843: VETVPMKNLEI → SSVSGMWHFTL
     844-867: Missing.

Show »
Length:834
Mass (Da):93,416
Checksum:iCAFC85B2596BAB9C
GO
Isoform 4 (identifier: O54990-4) [UniParc]FASTAAdd to basket
Also known as: S4

The sequence of this isoform differs from the canonical sequence as follows:
     94-107: Missing.
     335-360: QLPSVDRELNTVTEVDKTDLESLVKR → Q
     833-843: VETVPMKNLEI → SSVSGMWHFTL
     844-867: Missing.

Show »
Length:804
Mass (Da):89,987
Checksum:iA8C6925524AE94D5
GO
Isoform 5 (identifier: O54990-5) [UniParc]FASTAAdd to basket
Also known as: S5

The sequence of this isoform differs from the canonical sequence as follows:
     94-102: Missing.
     335-360: QLPSVDRELNTVTEVDKTDLESLVKR → Q
     833-843: VETVPMKNLEI → SSVSGMWHFTL
     844-867: Missing.

Show »
Length:809
Mass (Da):90,577
Checksum:i1FF6FB4EF6CB7095
GO
Isoform 6 (identifier: O54990-6) [UniParc]FASTAAdd to basket
Also known as: S6

The sequence of this isoform differs from the canonical sequence as follows:
     94-102: Missing.
     832-832: D → E
     833-867: Missing.

Show »
Length:823
Mass (Da):92,197
Checksum:iE8425D90B970938A
GO

Computationally mapped potential isoform sequencesi

There are 10 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G5E8G5G5E8G5_MOUSE
Prominin 1, isoform CRA_a
Prom1 mCG_124143
867Annotation score:
Q8R056Q8R056_MOUSE
Prom1 protein
Prom1
842Annotation score:
Q8CDK8Q8CDK8_MOUSE
Prominin-1
Prom1
827Annotation score:
A0A0G2JE50A0A0G2JE50_MOUSE
Prominin-1
Prom1
804Annotation score:
G3X9J8G3X9J8_MOUSE
Prominin 1, isoform CRA_g
Prom1 mCG_124143
858Annotation score:
A0A0R4J0B0A0A0R4J0B0_MOUSE
Prominin-1
Prom1
809Annotation score:
A0A0G2JDH9A0A0G2JDH9_MOUSE
Prominin-1
Prom1
834Annotation score:
A0A0G2JG30A0A0G2JG30_MOUSE
Prominin-1
Prom1
98Annotation score:
A0A0G2JDS7A0A0G2JDS7_MOUSE
Prominin-1
Prom1
63Annotation score:
A0A0G2JE90A0A0G2JE90_MOUSE
Prominin-1
Prom1
190Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti64S → N in AAB86715 (PubMed:9356465).Curated1
Sequence conflicti64S → N in AAM28245 (PubMed:15316084).Curated1
Sequence conflicti64S → N in AAO11840 (PubMed:15316084).Curated1
Sequence conflicti64S → N in AAO72429 (PubMed:15316084).Curated1
Sequence conflicti64S → N in AAO72430 (PubMed:15316084).Curated1
Sequence conflicti64S → N in BAC26745 (PubMed:16141072).Curated1
Sequence conflicti84K → N in AAB86715 (PubMed:9356465).Curated1
Sequence conflicti84K → N in AAM28245 (PubMed:15316084).Curated1
Sequence conflicti84K → N in AAO11840 (PubMed:15316084).Curated1
Sequence conflicti84K → N in AAO72429 (PubMed:15316084).Curated1
Sequence conflicti84K → N in AAO72430 (PubMed:15316084).Curated1
Sequence conflicti84K → N in BAC26745 (PubMed:16141072).Curated1
Sequence conflicti668P → L in AAB86715 (PubMed:9356465).Curated1
Sequence conflicti668P → L in AAM28245 (PubMed:15316084).Curated1
Sequence conflicti668P → L in AAO72429 (PubMed:15316084).Curated1
Sequence conflicti668P → L in AAO72430 (PubMed:15316084).Curated1
Sequence conflicti668P → L in BAC26745 (PubMed:16141072).Curated1
Sequence conflicti844G → D in AAB86715 (PubMed:9356465).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04000594 – 107Missing in isoform 4. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_04000694 – 102Missing in isoform 2, isoform 3, isoform 5 and isoform 6. 3 Publications9
Alternative sequenceiVSP_040007335 – 360QLPSV…SLVKR → Q in isoform 4 and isoform 5. 1 PublicationAdd BLAST26
Alternative sequenceiVSP_040008832D → E in isoform 6. 1 Publication1
Alternative sequenceiVSP_040009833 – 867Missing in isoform 6. 1 PublicationAdd BLAST35
Alternative sequenceiVSP_040010833 – 843VETVPMKNLEI → SSVSGMWHFTL in isoform 3, isoform 4 and isoform 5. 2 PublicationsAdd BLAST11
Alternative sequenceiVSP_040011844 – 867Missing in isoform 3, isoform 4 and isoform 5. 2 PublicationsAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039663 mRNA Translation: AAB96916.1
AF026269 mRNA Translation: AAB86715.1
AF305215 mRNA Translation: AAO11840.1
AY099088 mRNA Translation: AAM28245.1
AY223521 mRNA Translation: AAO72429.1
AY223522 mRNA Translation: AAO72430.1
AK030027 mRNA Translation: BAC26745.1
CCDSiCCDS39082.1 [O54990-2]
CCDS51491.1 [O54990-5]
CCDS51493.1 [O54990-3]
CCDS51494.1 [O54990-1]
CCDS80275.1 [O54990-4]
PIRiT08881
RefSeqiNP_001157050.1, NM_001163578.1
NP_001157053.1, NM_001163581.1
NP_001157054.1, NM_001163582.1
NP_001157055.1, NM_001163583.1
UniGeneiMm.6250

Genome annotation databases

GeneIDi19126
KEGGimmu:19126
UCSCiuc008xii.1 mouse [O54990-4]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039663 mRNA Translation: AAB96916.1
AF026269 mRNA Translation: AAB86715.1
AF305215 mRNA Translation: AAO11840.1
AY099088 mRNA Translation: AAM28245.1
AY223521 mRNA Translation: AAO72429.1
AY223522 mRNA Translation: AAO72430.1
AK030027 mRNA Translation: BAC26745.1
CCDSiCCDS39082.1 [O54990-2]
CCDS51491.1 [O54990-5]
CCDS51493.1 [O54990-3]
CCDS51494.1 [O54990-1]
CCDS80275.1 [O54990-4]
PIRiT08881
RefSeqiNP_001157050.1, NM_001163578.1
NP_001157053.1, NM_001163581.1
NP_001157054.1, NM_001163582.1
NP_001157055.1, NM_001163583.1
UniGeneiMm.6250

3D structure databases

ProteinModelPortaliO54990
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202393, 1 interactor
IntActiO54990, 1 interactor
MINTiO54990
STRINGi10090.ENSMUSP00000073751

PTM databases

iPTMnetiO54990
PhosphoSitePlusiO54990

Proteomic databases

MaxQBiO54990
PaxDbiO54990
PeptideAtlasiO54990
PRIDEiO54990

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi19126
KEGGimmu:19126
UCSCiuc008xii.1 mouse [O54990-4]

Organism-specific databases

CTDi8842
MGIiMGI:1100886 Prom1

Phylogenomic databases

eggNOGiKOG4331 Eukaryota
ENOG410XRG0 LUCA
HOGENOMiHOG000115704
HOVERGENiHBG053690
InParanoidiO54990
KOiK06532
PhylomeDBiO54990

Miscellaneous databases

ChiTaRSiProm1 mouse
PROiPR:O54990
SOURCEiSearch...

Gene expression databases

CleanExiMM_PROM1

Family and domain databases

InterProiView protein in InterPro
IPR008795 Prominin
PANTHERiPTHR22730 PTHR22730, 1 hit
PfamiView protein in Pfam
PF05478 Prominin, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPROM1_MOUSE
AccessioniPrimary (citable) accession number: O54990
Secondary accession number(s): O35408
, Q80XB2, Q80XB3, Q80XB6, Q8BH12
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: June 1, 1998
Last modified: November 7, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again