UniProtKB - O54984 (ASNA_MOUSE)
Protein
ATPase Asna1
Gene
Asna1
Organism
Mus musculus (Mouse)
Status
Functioni
ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 74 | UniRule annotation | 1 | |
Binding sitei | 251 | ATPUniRule annotation | 1 | |
Binding sitei | 278 | ATPUniRule annotation | 1 | |
Metal bindingi | 289 | Zinc; shared with dimeric partnerUniRule annotation | 1 | |
Metal bindingi | 292 | Zinc; shared with dimeric partnerUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 45 – 52 | ATPUniRule annotation | 8 |
GO - Molecular functioni
- ATPase activity Source: GO_Central
- ATP binding Source: UniProtKB-UniRule
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- posttranslational protein targeting to endoplasmic reticulum membrane Source: GO_Central
- tail-anchored membrane protein insertion into ER membrane Source: UniProtKB
Keywordsi
Molecular function | Hydrolase |
Biological process | Transport |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-381038 XBP1(S) activates chaperone genes |
Names & Taxonomyi
Protein namesi | Recommended name: ATPase Asna1UniRule annotation (EC:3.6.-.-UniRule annotation)Alternative name(s): Arsenical pump-driving ATPaseUniRule annotation Arsenite-stimulated ATPaseUniRule annotation |
Gene namesi | Name:Asna1 Synonyms:Arsa |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1928379 Asna1 |
Subcellular locationi
Nucleus
- nucleolus UniRule annotation
Endoplasmic reticulum
- Endoplasmic reticulum UniRule annotation
Other locations
- Cytoplasm UniRule annotation
Endoplasmic reticulum
- GET complex Source: GO_Central
Nucleus
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, NucleusPathology & Biotechi
Disruption phenotypei
Causes early embryonic lethality.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000152254 | 2 – 348 | ATPase Asna1Add BLAST | 347 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | O54984 |
jPOSTi | O54984 |
MaxQBi | O54984 |
PaxDbi | O54984 |
PeptideAtlasi | O54984 |
PRIDEi | O54984 |
2D gel databases
REPRODUCTION-2DPAGEi | O54984 |
PTM databases
iPTMneti | O54984 |
PhosphoSitePlusi | O54984 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000052456 Expressed in 301 organ(s), highest expression level in blood |
ExpressionAtlasi | O54984 baseline and differential |
Genevisiblei | O54984 MM |
Interactioni
Subunit structurei
Homodimer. Component of a transmembrane domain recognition complex (TRC). Interacts with SERP1 and SEC61B. Interacts with WRB.UniRule annotation
Protein-protein interaction databases
BioGridi | 208019, 8 interactors |
IntActi | O54984, 7 interactors |
MINTi | O54984 |
STRINGi | 10090.ENSMUSP00000065337 |
Structurei
3D structure databases
ProteinModelPortali | O54984 |
SMRi | O54984 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the arsA ATPase family.UniRule annotation
Phylogenomic databases
eggNOGi | KOG2825 Eukaryota COG0003 LUCA |
GeneTreei | ENSGT00390000003817 |
HOGENOMi | HOG000197637 |
InParanoidi | O54984 |
KOi | K01551 |
OMAi | MDAPYEF |
OrthoDBi | 992208at2759 |
PhylomeDBi | O54984 |
TreeFami | TF300670 |
Family and domain databases
CDDi | cd02035 ArsA, 1 hit |
HAMAPi | MF_03112 Asna1_Get3, 1 hit |
InterProi | View protein in InterPro IPR025723 Anion-transp_ATPase-like_dom IPR016300 ATPase_ArsA/GET3 IPR027542 ATPase_ArsA/GET3_euk IPR027417 P-loop_NTPase |
PANTHERi | PTHR10803 PTHR10803, 1 hit PTHR10803:SF3 PTHR10803:SF3, 1 hit |
Pfami | View protein in Pfam PF02374 ArsA_ATPase, 1 hit |
SUPFAMi | SSF52540 SSF52540, 1 hit |
TIGRFAMsi | TIGR00345 GET3_arsA_TRC40, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O54984-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAGVAGWGV EAEEFEDAPD VEPLEPTLSN IIEQRSLKWI FVGGKGGVGK
60 70 80 90 100
TTCSCSLAVQ LSKGRESVLI ISTDPAHNIS DAFDQKFSKV PTKVKGYDNL
110 120 130 140 150
FAMEIDPSLG VAELPDEFFE EDNMLSMGKK MMQEAMSAFP GIDEAMSYAE
160 170 180 190 200
VMRLVKGMNF SVVVFDTAPT GHTLRLLNFP TIVERGLGRL MQIKNQISPF
210 220 230 240 250
ISQMCNMLGL GDMNADQLAS KLEETLPVIR SVSEQFKDPE QTTFICVCIA
260 270 280 290 300
EFLSLYETER LIQELAKCKI DTHNIIVNQL VFPDPEKPCK MCEARHKIQA
310 320 330 340
KYLDQMEDLY EDFHIVKLPL LPHEVRGADK VNTFSALLLE PYKPPSTQ
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1B0GRE1 | A0A1B0GRE1_MOUSE | ATPase Asna1 | Asna1 | 131 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF039405 mRNA Translation: AAB94772.2 AF061177 Genomic DNA Translation: AAD15826.2 BC016453 mRNA Translation: AAH16453.1 BC083335 mRNA Translation: AAH83335.1 AK171690 mRNA Translation: BAE42614.1 CH466525 Genomic DNA Translation: EDL10985.1 |
CCDSi | CCDS40417.1 |
RefSeqi | NP_062626.1, NM_019652.1 |
UniGenei | Mm.41475 |
Genome annotation databases
Ensembli | ENSMUST00000064314; ENSMUSP00000065337; ENSMUSG00000052456 |
GeneIDi | 56495 |
KEGGi | mmu:56495 |
UCSCi | uc009mox.1 mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF039405 mRNA Translation: AAB94772.2 AF061177 Genomic DNA Translation: AAD15826.2 BC016453 mRNA Translation: AAH16453.1 BC083335 mRNA Translation: AAH83335.1 AK171690 mRNA Translation: BAE42614.1 CH466525 Genomic DNA Translation: EDL10985.1 |
CCDSi | CCDS40417.1 |
RefSeqi | NP_062626.1, NM_019652.1 |
UniGenei | Mm.41475 |
3D structure databases
ProteinModelPortali | O54984 |
SMRi | O54984 |
ModBasei | Search... |
MobiDBi | Search... |
Protein-protein interaction databases
BioGridi | 208019, 8 interactors |
IntActi | O54984, 7 interactors |
MINTi | O54984 |
STRINGi | 10090.ENSMUSP00000065337 |
PTM databases
iPTMneti | O54984 |
PhosphoSitePlusi | O54984 |
2D gel databases
REPRODUCTION-2DPAGEi | O54984 |
Proteomic databases
EPDi | O54984 |
jPOSTi | O54984 |
MaxQBi | O54984 |
PaxDbi | O54984 |
PeptideAtlasi | O54984 |
PRIDEi | O54984 |
Protocols and materials databases
DNASUi | 56495 |
Structural Biology Knowledgebase | Search... |
Genome annotation databases
Ensembli | ENSMUST00000064314; ENSMUSP00000065337; ENSMUSG00000052456 |
GeneIDi | 56495 |
KEGGi | mmu:56495 |
UCSCi | uc009mox.1 mouse |
Organism-specific databases
CTDi | 439 |
MGIi | MGI:1928379 Asna1 |
Phylogenomic databases
eggNOGi | KOG2825 Eukaryota COG0003 LUCA |
GeneTreei | ENSGT00390000003817 |
HOGENOMi | HOG000197637 |
InParanoidi | O54984 |
KOi | K01551 |
OMAi | MDAPYEF |
OrthoDBi | 992208at2759 |
PhylomeDBi | O54984 |
TreeFami | TF300670 |
Enzyme and pathway databases
Reactomei | R-MMU-381038 XBP1(S) activates chaperone genes |
Miscellaneous databases
PROi | PR:O54984 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000052456 Expressed in 301 organ(s), highest expression level in blood |
ExpressionAtlasi | O54984 baseline and differential |
Genevisiblei | O54984 MM |
Family and domain databases
CDDi | cd02035 ArsA, 1 hit |
HAMAPi | MF_03112 Asna1_Get3, 1 hit |
InterProi | View protein in InterPro IPR025723 Anion-transp_ATPase-like_dom IPR016300 ATPase_ArsA/GET3 IPR027542 ATPase_ArsA/GET3_euk IPR027417 P-loop_NTPase |
PANTHERi | PTHR10803 PTHR10803, 1 hit PTHR10803:SF3 PTHR10803:SF3, 1 hit |
Pfami | View protein in Pfam PF02374 ArsA_ATPase, 1 hit |
SUPFAMi | SSF52540 SSF52540, 1 hit |
TIGRFAMsi | TIGR00345 GET3_arsA_TRC40, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | ASNA_MOUSE | |
Accessioni | O54984Primary (citable) accession number: O54984 Secondary accession number(s): Q3TAQ4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | July 11, 2001 | |
Last modified: | February 13, 2019 | |
This is version 152 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot