Asna1

Functionⁱ

ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.UniRule annotation

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	74	UniRule annotation	1
Binding siteⁱ	251	ATPUniRule annotation	1
Binding siteⁱ	278	ATPUniRule annotation	1
Metal bindingⁱ	289	Zinc; shared with dimeric partnerUniRule annotation	1
Metal bindingⁱ	292	Zinc; shared with dimeric partnerUniRule annotation	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	45 – 52	ATPUniRule annotation		8

GO - Molecular functionⁱ

ATPase activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
ATP binding Source: UniProtKB-UniRule
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

posttranslational protein targeting to endoplasmic reticulum membrane
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
tail-anchored membrane protein insertion into ER membrane Source: UniProtKB

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase
Biological process	Transport
Ligand	ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

Reactomeⁱ	R-MMU-381038 XBP1(S) activates chaperone genes

Reactomeⁱ

R-MMU-381038 XBP1(S) activates chaperone genes

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: ATPase Asna1UniRule annotation (EC:3.6.-.-UniRule annotation) Alternative name(s): Arsenical pump-driving ATPaseUniRule annotation Arsenite-stimulated ATPaseUniRule annotation
Gene namesⁱ	Name:Asna1 Synonyms:Arsa
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 8

Organism-specific databases

MGIⁱ	MGI:1928379 Asna1

Keywords - Cellular componentⁱ

Cytoplasm, Endoplasmic reticulum, Nucleus

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Causes early embryonic lethality.1 Publication

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedBy similarity
ChainⁱPRO_0000152254	2 – 348	ATPase Asna1Add BLAST		347

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	2	N-acetylalanineBy similarity		1

Keywords - PTMⁱ

Acetylation

Proteomic databases

EPDⁱ	O54984
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O54984
PaxDbⁱ	O54984
PeptideAtlas More...PeptideAtlasⁱ	O54984
PRIDEⁱ	O54984

2D gel databases

REPRODUCTION-2DPAGE More...REPRODUCTION-2DPAGEⁱ	O54984

REPRODUCTION-2DPAGEⁱ

O54984

PTM databases

iPTMnetⁱ	O54984
PhosphoSitePlusⁱ	O54984

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000052456 Expressed in 301 organ(s), highest expression level in blood
CleanExⁱ	MM_ARSA
ExpressionAtlasⁱ	O54984 baseline and differential
Genevisibleⁱ	O54984 MM

Interactionⁱ

Subunit structureⁱ

Homodimer. Component of a transmembrane domain recognition complex (TRC). Interacts with SERP1 and SEC61B. Interacts with WRB.UniRule annotation

Protein-protein interaction databases

BioGridⁱ	208019, 8 interactors
IntActⁱ	O54984, 7 interactors
Molecular INTeraction database More...MINTⁱ	O54984
STRINGⁱ	10090.ENSMUSP00000065337

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O54984
SMRⁱ	O54984
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the arsA ATPase family.UniRule annotation

Phylogenomic databases

eggNOGⁱ	KOG2825 Eukaryota COG0003 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000003817
HOGENOMⁱ	HOG000197637
InParanoidⁱ	O54984
KEGG Orthology (KO) More...KOⁱ	K01551
OMAⁱ	MDAPYEF
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0CAR
PhylomeDBⁱ	O54984
TreeFamⁱ	TF300670

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd02035 ArsA, 1 hit
HAMAPⁱ	MF_03112 Asna1_Get3, 1 hit
InterProⁱ	View protein in InterPro IPR025723 Anion-transp_ATPase-like_dom IPR016300 ATPase_ArsA/GET3 IPR027542 ATPase_ArsA/GET3_euk IPR027417 P-loop_NTPase
PANTHERⁱ	PTHR10803 PTHR10803, 1 hit PTHR10803:SF3 PTHR10803:SF3, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02374 ArsA_ATPase, 1 hit
SUPFAMⁱ	SSF52540 SSF52540, 1 hit
TIGRFAMsⁱ	TIGR00345 GET3_arsA_TRC40, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

O54984-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAAGVAGWGV EAEEFEDAPD VEPLEPTLSN IIEQRSLKWI FVGGKGGVGK 
        60         70         80         90        100
TTCSCSLAVQ LSKGRESVLI ISTDPAHNIS DAFDQKFSKV PTKVKGYDNL 
       110        120        130        140        150
FAMEIDPSLG VAELPDEFFE EDNMLSMGKK MMQEAMSAFP GIDEAMSYAE 
       160        170        180        190        200
VMRLVKGMNF SVVVFDTAPT GHTLRLLNFP TIVERGLGRL MQIKNQISPF 
       210        220        230        240        250
ISQMCNMLGL GDMNADQLAS KLEETLPVIR SVSEQFKDPE QTTFICVCIA 
       260        270        280        290        300
EFLSLYETER LIQELAKCKI DTHNIIVNQL VFPDPEKPCK MCEARHKIQA 
       310        320        330        340 
KYLDQMEDLY EDFHIVKLPL LPHEVRGADK VNTFSALLLE PYKPPSTQ

Length:348

Mass (Da):38,823

Last modified:July 11, 2001 - v2

Checksum:ⁱDA4EB4ACC35C7A36

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	A0A1B0GRE1	A0A1B0GRE1_MOUSE		ATPase Asna1 ATPase Asna1	Asna1	131	Annotation score:

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF039405 mRNA Translation: AAB94772.2 AF061177 Genomic DNA Translation: AAD15826.2 BC016453 mRNA Translation: AAH16453.1 BC083335 mRNA Translation: AAH83335.1 AK171690 mRNA Translation: BAE42614.1 CH466525 Genomic DNA Translation: EDL10985.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS40417.1
NCBI Reference Sequences More...RefSeqⁱ	NP_062626.1, NM_019652.1
UniGeneⁱ	Mm.41475

Genome annotation databases

Ensemblⁱ	ENSMUST00000064314; ENSMUSP00000065337; ENSMUSG00000052456
GeneIDⁱ	56495
KEGGⁱ	mmu:56495
UCSC genome browser More...UCSCⁱ	uc009mox.1 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O54984	ATPase ASNA1		TARSY		348	UniRef100_O54984
ATPase LOC101357183		Florida manatee		348
ATPase Asna1		RAT		348
ATPase ASNA1		CRIGR		348
Arsenical pump-driving ATPase		MOUSE		218
+2

Protein	Similar proteins		Species	Score	Length	Source
O54984	ATPase ASNA1		HUMAN		348	UniRef90_O43681
ATPase ASNA1		BOVIN		348
ATPase asna1		XENLA		342
ATPase asna1		XENTR		342
ATPase asna1		DANRE		341
+200

Protein	Similar proteins		Species	Score	Length	Source
O54984	ATPase ASNA1		HUMAN		348	UniRef50_O43681
ATPase ASNA1		BOVIN		348
ATPase asna1		XENTR		342
ATPase asna1		XENLA		342
ATPase asna1		DANRE		341
+240

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF039405 mRNA Translation: AAB94772.2 AF061177 Genomic DNA Translation: AAD15826.2 BC016453 mRNA Translation: AAH16453.1 BC083335 mRNA Translation: AAH83335.1 AK171690 mRNA Translation: BAE42614.1 CH466525 Genomic DNA Translation: EDL10985.1
CCDSⁱ	CCDS40417.1
RefSeqⁱ	NP_062626.1, NM_019652.1
UniGeneⁱ	Mm.41475

3D structure databases

ProteinModelPortalⁱ	O54984
SMRⁱ	O54984
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	208019, 8 interactors
IntActⁱ	O54984, 7 interactors
MINTⁱ	O54984
STRINGⁱ	10090.ENSMUSP00000065337

PTM databases

iPTMnetⁱ	O54984
PhosphoSitePlusⁱ	O54984

2D gel databases

REPRODUCTION-2DPAGEⁱ	O54984

REPRODUCTION-2DPAGEⁱ

O54984

Proteomic databases

EPDⁱ	O54984
MaxQBⁱ	O54984
PaxDbⁱ	O54984
PeptideAtlasⁱ	O54984
PRIDEⁱ	O54984

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	56495
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000064314; ENSMUSP00000065337; ENSMUSG00000052456
GeneIDⁱ	56495
KEGGⁱ	mmu:56495
UCSCⁱ	uc009mox.1 mouse

Organism-specific databases

CTDⁱ	439
MGIⁱ	MGI:1928379 Asna1

Phylogenomic databases

eggNOGⁱ	KOG2825 Eukaryota COG0003 LUCA
GeneTreeⁱ	ENSGT00390000003817
HOGENOMⁱ	HOG000197637
InParanoidⁱ	O54984
KOⁱ	K01551
OMAⁱ	MDAPYEF
OrthoDBⁱ	EOG091G0CAR
PhylomeDBⁱ	O54984
TreeFamⁱ	TF300670

Enzyme and pathway databases

Reactomeⁱ	R-MMU-381038 XBP1(S) activates chaperone genes

Reactomeⁱ

R-MMU-381038 XBP1(S) activates chaperone genes

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:O54984
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000052456 Expressed in 301 organ(s), highest expression level in blood
CleanExⁱ	MM_ARSA
ExpressionAtlasⁱ	O54984 baseline and differential
Genevisibleⁱ	O54984 MM

Family and domain databases

CDDⁱ	cd02035 ArsA, 1 hit
HAMAPⁱ	MF_03112 Asna1_Get3, 1 hit
InterProⁱ	View protein in InterPro IPR025723 Anion-transp_ATPase-like_dom IPR016300 ATPase_ArsA/GET3 IPR027542 ATPase_ArsA/GET3_euk IPR027417 P-loop_NTPase
PANTHERⁱ	PTHR10803 PTHR10803, 1 hit PTHR10803:SF3 PTHR10803:SF3, 1 hit
Pfamⁱ	View protein in Pfam PF02374 ArsA_ATPase, 1 hit
SUPFAMⁱ	SSF52540 SSF52540, 1 hit
TIGRFAMsⁱ	TIGR00345 GET3_arsA_TRC40, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ASNA_MOUSE
Accessionⁱ	O54984Primary (citable) accession number: O54984 Secondary accession number(s): Q3TAQ4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
	Last sequence update:	July 11, 2001
	Last modified:	November 7, 2018
	This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - O54984 (ASNA_MOUSE)

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ATPase Asna1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Endoplasmic reticulum

Nucleus

Other locations

Endoplasmic reticulum

Nucleus

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

2D gel databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ