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Protein

ATPase Asna1

Gene

Asna1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei74UniRule annotation1
Binding sitei251ATPUniRule annotation1
Binding sitei278ATPUniRule annotation1
Metal bindingi289Zinc; shared with dimeric partnerUniRule annotation1
Metal bindingi292Zinc; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi45 – 52ATPUniRule annotation8

GO - Molecular functioni

  • ATPase activity Source: GO_Central
  • ATP binding Source: UniProtKB-UniRule
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processTransport
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-381038 XBP1(S) activates chaperone genes

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase Asna1UniRule annotation (EC:3.6.-.-UniRule annotation)
Alternative name(s):
Arsenical pump-driving ATPaseUniRule annotation
Arsenite-stimulated ATPaseUniRule annotation
Gene namesi
Name:Asna1
Synonyms:Arsa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1928379 Asna1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Nucleus

Pathology & Biotechi

Disruption phenotypei

Causes early embryonic lethality.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001522542 – 348ATPase Asna1Add BLAST347

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO54984
MaxQBiO54984
PaxDbiO54984
PeptideAtlasiO54984
PRIDEiO54984

2D gel databases

REPRODUCTION-2DPAGEiO54984

PTM databases

iPTMnetiO54984
PhosphoSitePlusiO54984

Expressioni

Gene expression databases

BgeeiENSMUSG00000052456 Expressed in 301 organ(s), highest expression level in blood
CleanExiMM_ARSA
ExpressionAtlasiO54984 baseline and differential
GenevisibleiO54984 MM

Interactioni

Subunit structurei

Homodimer. Component of a transmembrane domain recognition complex (TRC). Interacts with SERP1 and SEC61B. Interacts with WRB.UniRule annotation

Protein-protein interaction databases

BioGridi208019, 8 interactors
IntActiO54984, 7 interactors
MINTiO54984
STRINGi10090.ENSMUSP00000065337

Structurei

3D structure databases

ProteinModelPortaliO54984
SMRiO54984
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the arsA ATPase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2825 Eukaryota
COG0003 LUCA
GeneTreeiENSGT00390000003817
HOGENOMiHOG000197637
InParanoidiO54984
KOiK01551
OMAiMDAPYEF
OrthoDBiEOG091G0CAR
PhylomeDBiO54984
TreeFamiTF300670

Family and domain databases

CDDicd02035 ArsA, 1 hit
HAMAPiMF_03112 Asna1_Get3, 1 hit
InterProiView protein in InterPro
IPR025723 Anion-transp_ATPase-like_dom
IPR016300 ATPase_ArsA/GET3
IPR027542 ATPase_ArsA/GET3_euk
IPR027417 P-loop_NTPase
PANTHERiPTHR10803 PTHR10803, 1 hit
PTHR10803:SF3 PTHR10803:SF3, 1 hit
PfamiView protein in Pfam
PF02374 ArsA_ATPase, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00345 GET3_arsA_TRC40, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O54984-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAGVAGWGV EAEEFEDAPD VEPLEPTLSN IIEQRSLKWI FVGGKGGVGK
60 70 80 90 100
TTCSCSLAVQ LSKGRESVLI ISTDPAHNIS DAFDQKFSKV PTKVKGYDNL
110 120 130 140 150
FAMEIDPSLG VAELPDEFFE EDNMLSMGKK MMQEAMSAFP GIDEAMSYAE
160 170 180 190 200
VMRLVKGMNF SVVVFDTAPT GHTLRLLNFP TIVERGLGRL MQIKNQISPF
210 220 230 240 250
ISQMCNMLGL GDMNADQLAS KLEETLPVIR SVSEQFKDPE QTTFICVCIA
260 270 280 290 300
EFLSLYETER LIQELAKCKI DTHNIIVNQL VFPDPEKPCK MCEARHKIQA
310 320 330 340
KYLDQMEDLY EDFHIVKLPL LPHEVRGADK VNTFSALLLE PYKPPSTQ
Length:348
Mass (Da):38,823
Last modified:July 11, 2001 - v2
Checksum:iDA4EB4ACC35C7A36
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1B0GRE1A0A1B0GRE1_MOUSE
ATPase Asna1
Asna1
131Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039405 mRNA Translation: AAB94772.2
AF061177 Genomic DNA Translation: AAD15826.2
BC016453 mRNA Translation: AAH16453.1
BC083335 mRNA Translation: AAH83335.1
AK171690 mRNA Translation: BAE42614.1
CH466525 Genomic DNA Translation: EDL10985.1
CCDSiCCDS40417.1
RefSeqiNP_062626.1, NM_019652.1
UniGeneiMm.41475

Genome annotation databases

EnsembliENSMUST00000064314; ENSMUSP00000065337; ENSMUSG00000052456
GeneIDi56495
KEGGimmu:56495
UCSCiuc009mox.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039405 mRNA Translation: AAB94772.2
AF061177 Genomic DNA Translation: AAD15826.2
BC016453 mRNA Translation: AAH16453.1
BC083335 mRNA Translation: AAH83335.1
AK171690 mRNA Translation: BAE42614.1
CH466525 Genomic DNA Translation: EDL10985.1
CCDSiCCDS40417.1
RefSeqiNP_062626.1, NM_019652.1
UniGeneiMm.41475

3D structure databases

ProteinModelPortaliO54984
SMRiO54984
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208019, 8 interactors
IntActiO54984, 7 interactors
MINTiO54984
STRINGi10090.ENSMUSP00000065337

PTM databases

iPTMnetiO54984
PhosphoSitePlusiO54984

2D gel databases

REPRODUCTION-2DPAGEiO54984

Proteomic databases

EPDiO54984
MaxQBiO54984
PaxDbiO54984
PeptideAtlasiO54984
PRIDEiO54984

Protocols and materials databases

DNASUi56495
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064314; ENSMUSP00000065337; ENSMUSG00000052456
GeneIDi56495
KEGGimmu:56495
UCSCiuc009mox.1 mouse

Organism-specific databases

CTDi439
MGIiMGI:1928379 Asna1

Phylogenomic databases

eggNOGiKOG2825 Eukaryota
COG0003 LUCA
GeneTreeiENSGT00390000003817
HOGENOMiHOG000197637
InParanoidiO54984
KOiK01551
OMAiMDAPYEF
OrthoDBiEOG091G0CAR
PhylomeDBiO54984
TreeFamiTF300670

Enzyme and pathway databases

ReactomeiR-MMU-381038 XBP1(S) activates chaperone genes

Miscellaneous databases

PROiPR:O54984
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000052456 Expressed in 301 organ(s), highest expression level in blood
CleanExiMM_ARSA
ExpressionAtlasiO54984 baseline and differential
GenevisibleiO54984 MM

Family and domain databases

CDDicd02035 ArsA, 1 hit
HAMAPiMF_03112 Asna1_Get3, 1 hit
InterProiView protein in InterPro
IPR025723 Anion-transp_ATPase-like_dom
IPR016300 ATPase_ArsA/GET3
IPR027542 ATPase_ArsA/GET3_euk
IPR027417 P-loop_NTPase
PANTHERiPTHR10803 PTHR10803, 1 hit
PTHR10803:SF3 PTHR10803:SF3, 1 hit
PfamiView protein in Pfam
PF02374 ArsA_ATPase, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00345 GET3_arsA_TRC40, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiASNA_MOUSE
AccessioniPrimary (citable) accession number: O54984
Secondary accession number(s): Q3TAQ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 11, 2001
Last modified: November 7, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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