Banf1

Functionⁱ

Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging.By similarity

GO - Molecular functionⁱ

DNA binding
Source: MGI
Inferred from direct assayⁱ
- 9465049
enzyme binding Source: MGI
identical protein binding Source: MGI
LEM domain binding Source: MGI
protein C-terminus binding Source: MGI
protein homodimerization activity Source: MGI
protein N-terminus binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

DNA integration
Source: MGI
Inferred from direct assayⁱ
- 9465049
establishment of integrated proviral latency
Source: MGI
Traceable author statementⁱ
- 11591818
negative regulation of viral genome replication Source: MGI

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding

Molecular function

DNA-binding

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Barrier-to-autointegration factor Alternative name(s): Breakpoint cluster region protein 1 LAP2-binding protein 1 Cleaved into the following chain: Barrier-to-autointegration factor, N-terminally processed
Gene namesⁱ	Name:Banf1 Synonyms:Baf, Bcrp1, L2bp1
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 19

Organism-specific databases

MGIⁱ	MGI:1346330 Banf1

Keywords - Cellular componentⁱ

Chromosome, Cytoplasm, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Length
ChainⁱPRO_0000423191	1 – 89	Barrier-to-autointegration factorAdd BLAST	89
Initiator methionineⁱ		Removed; alternateBy similarity
ChainⁱPRO_0000221027	2 – 89	Barrier-to-autointegration factor, N-terminally processedAdd BLAST	88

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	1	N-acetylmethionineBy similarity	1
Modified residueⁱ	2	N-acetylthreonine; in Barrier-to-autointegration factor, N-terminally processedBy similarity	1
Modified residueⁱ	2	Phosphothreonine; by VRK1 and VRK2By similarity	1
Modified residueⁱ	3	Phosphothreonine; by VRK1 and VRK2By similarity	1
Modified residueⁱ	4	Phosphoserine; by VRK1 and VRK2By similarity	1

Post-translational modificationⁱ

Ser-4 is the major site of phosphorylation as compared to Thr-2 and Thr-3. Phosphorylation on Thr-2; Thr-3 and Ser-4 disrupts its ability to bind DNA and reduces its ability to bind LEM domain-containing proteins. Non phosphorylated BAF seems to enhance binding between EMD and LMNA. Dephosphorylated by protein phosphatase 2A (PP2A) following interaction with ANKLE2/LEM4 during mitotic exit, leading to mitotic nuclear envelope reassembly.By similarity

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

EPDⁱ	O54962
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O54962
PaxDbⁱ	O54962
PeptideAtlas More...PeptideAtlasⁱ	O54962
PRIDEⁱ	O54962
TopDownProteomicsⁱ	O54962

PTM databases

iPTMnetⁱ	O54962
PhosphoSitePlusⁱ	O54962

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000024844 Expressed in 288 organ(s), highest expression level in liver
CleanExⁱ	MM_BANF1
Genevisibleⁱ	O54962 MM

Interactionⁱ

Subunit structureⁱ

Homodimer. Heterodimerizes with BAFL. Interacts with ANKLE2/LEM4, leading to decreased phosphorylation by VRK1 and promoting dephosphorylation by protein phosphatase 2A (PP2A). Binds non-specifically to double-stranded DNA, and is found as a hexamer or dodecamer upon DNA binding. Binds to LEM domain-containing nuclear proteins such as LEMD3/MAN1, TMPO/LAP2 and EMD (emerin). Interacts with ANKLE1 (via LEM domain); the interaction may favor BANF1 dimerization. Interacts with CRX and LMNA (lamin-A). Binds linker histone H1.1 and core histones H3.By similarity

GO - Molecular functionⁱ

enzyme binding Source: MGI
identical protein binding Source: MGI
LEM domain binding Source: MGI
protein C-terminus binding Source: MGI
protein homodimerization activity Source: MGI
protein N-terminus binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	204742, 1 interactor
IntActⁱ	O54962, 4 interactors
Molecular INTeraction database More...MINTⁱ	O54962
STRINGⁱ	10090.ENSMUSP00000025762

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O54962
SMRⁱ	O54962
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	20 – 35	HhHBy similarityAdd BLAST		16

Domainⁱ

Has a helix-hairpin-helix (HhH) structural motif conserved among proteins that bind non-specifically to DNA.By similarity

LEM domain proteins bind centrally on the BAF dimer.By similarity

Sequence similaritiesⁱ

Belongs to the BAF family.Curated

Phylogenomic databases

eggNOGⁱ	KOG4233 Eukaryota ENOG4111URU LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000018613
HOGENOMⁱ	HOG000231927
HOVERGENⁱ	HBG029345
InParanoidⁱ	O54962
KEGG Orthology (KO) More...KOⁱ	K21870
OMAⁱ	CGANSKQ
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G109J
PhylomeDBⁱ	O54962
TreeFamⁱ	TF315060

Family and domain databases

Gene3Dⁱ	1.10.150.40, 1 hit
InterProⁱ	View protein in InterPro IPR004122 BAF_prot IPR036617 BAF_sf
PANTHERⁱ	PTHR12912 PTHR12912, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02961 BAF, 1 hit
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD019964 PD019964, 1 hit
SMARTⁱ	View protein in SMART SM01023 BAF, 1 hit
SUPFAMⁱ	SSF47798 SSF47798, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

O54962-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTTSQKHRDF VAEPMGEKPV GSLAGIGDVL SKRLEERGFD KAYVVLGQFL 
        60         70         80 
VLKKDEDLFR EWLKDTCGAN AKQSRDCFGC LREWCDAFL

Length:89

Mass (Da):10,103

Last modified:June 1, 1998 - v1

Checksum:ⁱ828924AFCB91E0D0

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF037080 mRNA Translation: AAC40032.1 AB025349 mRNA Translation: BAA83102.1 AK003025 mRNA Translation: BAB22518.1 AK004212 mRNA Translation: BAB23223.1 AK010257 mRNA Translation: BAB26800.1 AK011079 mRNA Translation: BAB27383.1 AK011100 mRNA Translation: BAB27397.1 AK012588 mRNA Translation: BAB28337.1 AK088896 mRNA Translation: BAC40639.1 BC003709 mRNA Translation: AAH03709.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS29458.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001033320.1, NM_001038231.2 NP_001273537.1, NM_001286608.1 NP_035923.1, NM_011793.3
UniGeneⁱ	Mm.358649

Genome annotation databases

Ensemblⁱ	ENSMUST00000025762; ENSMUSP00000025762; ENSMUSG00000024844 ENSMUST00000170010; ENSMUSP00000126202; ENSMUSG00000024844
GeneIDⁱ	23825
KEGGⁱ	mmu:23825
UCSC genome browser More...UCSCⁱ	uc008gcr.2 mouse

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O54962	Barrier-to-autointegration factor		RAT		89	UniRef90_O54962
Uncharacterized protein		Cebus capucinus imitator		90
cDNA, FLJ92232, highly similar to Homo sapiens barrier to autointegration factor 1 (BANF1), mRNA		HUMAN		89
BANF1		AILME		89
Uncharacterized protein		RHIRO		89
+35

Protein	Similar proteins		Species	Score	Length	Source
O54962	Barrier-to-autointegration factor		RAT		89	UniRef50_O54962
Uncharacterized protein		Cebus capucinus imitator		90
Uncharacterized protein		CERAT		89
barrier-to-autointegration factor-like		LEPWE		89
BANF1		AILME		89
+40

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF037080 mRNA Translation: AAC40032.1 AB025349 mRNA Translation: BAA83102.1 AK003025 mRNA Translation: BAB22518.1 AK004212 mRNA Translation: BAB23223.1 AK010257 mRNA Translation: BAB26800.1 AK011079 mRNA Translation: BAB27383.1 AK011100 mRNA Translation: BAB27397.1 AK012588 mRNA Translation: BAB28337.1 AK088896 mRNA Translation: BAC40639.1 BC003709 mRNA Translation: AAH03709.1
CCDSⁱ	CCDS29458.1
RefSeqⁱ	NP_001033320.1, NM_001038231.2 NP_001273537.1, NM_001286608.1 NP_035923.1, NM_011793.3
UniGeneⁱ	Mm.358649

3D structure databases

ProteinModelPortalⁱ	O54962
SMRⁱ	O54962
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	204742, 1 interactor
IntActⁱ	O54962, 4 interactors
MINTⁱ	O54962
STRINGⁱ	10090.ENSMUSP00000025762

PTM databases

iPTMnetⁱ	O54962
PhosphoSitePlusⁱ	O54962

Proteomic databases

EPDⁱ	O54962
MaxQBⁱ	O54962
PaxDbⁱ	O54962
PeptideAtlasⁱ	O54962
PRIDEⁱ	O54962
TopDownProteomicsⁱ	O54962

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000025762; ENSMUSP00000025762; ENSMUSG00000024844 ENSMUST00000170010; ENSMUSP00000126202; ENSMUSG00000024844
GeneIDⁱ	23825
KEGGⁱ	mmu:23825
UCSCⁱ	uc008gcr.2 mouse

Organism-specific databases

CTDⁱ	8815
MGIⁱ	MGI:1346330 Banf1

Phylogenomic databases

eggNOGⁱ	KOG4233 Eukaryota ENOG4111URU LUCA
GeneTreeⁱ	ENSGT00390000018613
HOGENOMⁱ	HOG000231927
HOVERGENⁱ	HBG029345
InParanoidⁱ	O54962
KOⁱ	K21870
OMAⁱ	CGANSKQ
OrthoDBⁱ	EOG091G109J
PhylomeDBⁱ	O54962
TreeFamⁱ	TF315060

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:O54962
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000024844 Expressed in 288 organ(s), highest expression level in liver
CleanExⁱ	MM_BANF1
Genevisibleⁱ	O54962 MM

Family and domain databases

Gene3Dⁱ	1.10.150.40, 1 hit
InterProⁱ	View protein in InterPro IPR004122 BAF_prot IPR036617 BAF_sf
PANTHERⁱ	PTHR12912 PTHR12912, 1 hit
Pfamⁱ	View protein in Pfam PF02961 BAF, 1 hit
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD019964 PD019964, 1 hit
SMARTⁱ	View protein in SMART SM01023 BAF, 1 hit
SUPFAMⁱ	SSF47798 SSF47798, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	BAF_MOUSE
Accessionⁱ	O54962Primary (citable) accession number: O54962 Secondary accession number(s): Q542E6
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
	Last sequence update:	June 1, 1998
	Last modified:	November 7, 2018
	This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - O54962 (BAF_MOUSE)

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Barrier-to-autointegration factor

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Cytosol

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ