UniProtKB - O54962 (BAF_MOUSE)
Barrier-to-autointegration factor
Banf1
Functioni
GO - Molecular functioni
- DNA binding Source: MGI
- enzyme binding Source: MGI
- identical protein binding Source: MGI
- LEM domain binding Source: MGI
- protein C-terminus binding Source: MGI
- protein homodimerization activity Source: MGI
- protein N-terminus binding Source: MGI
GO - Biological processi
- chromosome condensation Source: GO_Central
- chromosome segregation Source: GO_Central
- DNA integration Source: MGI
- establishment of integrated proviral latency Source: MGI
- negative regulation of viral genome replication Source: MGI
Keywordsi
Molecular function | DNA-binding |
Enzyme and pathway databases
Reactomei | R-MMU-2980766, Nuclear Envelope Breakdown R-MMU-2995383, Initiation of Nuclear Envelope (NE) Reformation |
Names & Taxonomyi
Protein namesi | Recommended name: Barrier-to-autointegration factorAlternative name(s): Breakpoint cluster region protein 1 LAP2-binding protein 1 Cleaved into the following chain: |
Gene namesi | Name:Banf1 Synonyms:Baf, Bcrp1, L2bp1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
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Organism-specific databases
MGIi | MGI:1346330, Banf1 |
Subcellular locationi
Nucleus
- Nucleus By similarity
- Nucleus envelope By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- Chromosome By similarity
Note: Significantly enriched at the nuclear inner membrane, diffusely throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase. The phosphorylated form (by VRK1) shows a cytoplasmic localization whereas the unphosphorylated form locates almost exclusively in the nucleus.By similarity
Cytosol
- cytosol Source: MGI
Nucleus
- nuclear envelope Source: UniProtKB-SubCell
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- condensed chromosome Source: MGI
- cytoplasm Source: MGI
Keywords - Cellular componenti
Chromosome, Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000423191 | 1 – 89 | Barrier-to-autointegration factorAdd BLAST | 89 | |
Initiator methioninei | Removed; alternateBy similarity | |||
ChainiPRO_0000221027 | 2 – 89 | Barrier-to-autointegration factor, N-terminally processedAdd BLAST | 88 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 2 | N-acetylthreonine; in Barrier-to-autointegration factor, N-terminally processedBy similarity | 1 | |
Modified residuei | 2 | Phosphothreonine; by VRK1 and VRK2By similarity | 1 | |
Modified residuei | 3 | Phosphothreonine; by VRK1 and VRK2By similarity | 1 | |
Modified residuei | 4 | Phosphoserine; by VRK1 and VRK2By similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | O54962 |
jPOSTi | O54962 |
MaxQBi | O54962 |
PaxDbi | O54962 |
PeptideAtlasi | O54962 |
PRIDEi | O54962 |
ProteomicsDBi | 273534 |
TopDownProteomicsi | O54962 |
PTM databases
iPTMneti | O54962 |
PhosphoSitePlusi | O54962 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000024844, Expressed in liver and 303 other tissues |
Genevisiblei | O54962, MM |
Interactioni
Subunit structurei
Homodimer. Heterodimerizes with BANF2.
Interacts with ANKLE2/LEM4, leading to decreased phosphorylation by VRK1 and promoting dephosphorylation by protein phosphatase 2A (PP2A). Binds non-specifically to double-stranded DNA, and is found as a hexamer or dodecamer upon DNA binding. Binds to LEM domain-containing nuclear proteins such as LEMD3/MAN1, TMPO/LAP2 and EMD (emerin).
Interacts with ANKLE1 (via LEM domain); the interaction may favor BANF1 dimerization.
Interacts with CRX and LMNA (lamin-A). Binds linker histone H1.1 and core histones H3.
By similarityGO - Molecular functioni
- enzyme binding Source: MGI
- identical protein binding Source: MGI
- LEM domain binding Source: MGI
- protein C-terminus binding Source: MGI
- protein homodimerization activity Source: MGI
- protein N-terminus binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204742, 17 interactors |
IntActi | O54962, 4 interactors |
MINTi | O54962 |
STRINGi | 10090.ENSMUSP00000126202 |
Miscellaneous databases
RNActi | O54962, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 20 – 35 | HhHBy similarityAdd BLAST | 16 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4233, Eukaryota |
GeneTreei | ENSGT00390000018613 |
HOGENOMi | CLU_167806_0_0_1 |
InParanoidi | O54962 |
OMAi | TDWCEEF |
OrthoDBi | 1617480at2759 |
PhylomeDBi | O54962 |
TreeFami | TF315060 |
Family and domain databases
Gene3Di | 1.10.150.40, 1 hit |
InterProi | View protein in InterPro IPR004122, BAF_prot IPR036617, BAF_sf |
PANTHERi | PTHR12912, PTHR12912, 1 hit |
Pfami | View protein in Pfam PF02961, BAF, 1 hit |
SMARTi | View protein in SMART SM01023, BAF, 1 hit |
SUPFAMi | SSF47798, SSF47798, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MTTSQKHRDF VAEPMGEKPV GSLAGIGDVL SKRLEERGFD KAYVVLGQFL
60 70 80
VLKKDEDLFR EWLKDTCGAN AKQSRDCFGC LREWCDAFL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF037080 mRNA Translation: AAC40032.1 AB025349 mRNA Translation: BAA83102.1 AK003025 mRNA Translation: BAB22518.1 AK004212 mRNA Translation: BAB23223.1 AK010257 mRNA Translation: BAB26800.1 AK011079 mRNA Translation: BAB27383.1 AK011100 mRNA Translation: BAB27397.1 AK012588 mRNA Translation: BAB28337.1 AK088896 mRNA Translation: BAC40639.1 BC003709 mRNA Translation: AAH03709.1 |
CCDSi | CCDS29458.1 |
RefSeqi | NP_001033320.1, NM_001038231.2 NP_001273537.1, NM_001286608.1 NP_035923.1, NM_011793.3 |
Genome annotation databases
Ensembli | ENSMUST00000025762; ENSMUSP00000025762; ENSMUSG00000024844 ENSMUST00000170010; ENSMUSP00000126202; ENSMUSG00000024844 ENSMUST00000237167; ENSMUSP00000158117; ENSMUSG00000024844 |
GeneIDi | 23825 |
KEGGi | mmu:23825 |
UCSCi | uc008gcr.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF037080 mRNA Translation: AAC40032.1 AB025349 mRNA Translation: BAA83102.1 AK003025 mRNA Translation: BAB22518.1 AK004212 mRNA Translation: BAB23223.1 AK010257 mRNA Translation: BAB26800.1 AK011079 mRNA Translation: BAB27383.1 AK011100 mRNA Translation: BAB27397.1 AK012588 mRNA Translation: BAB28337.1 AK088896 mRNA Translation: BAC40639.1 BC003709 mRNA Translation: AAH03709.1 |
CCDSi | CCDS29458.1 |
RefSeqi | NP_001033320.1, NM_001038231.2 NP_001273537.1, NM_001286608.1 NP_035923.1, NM_011793.3 |
3D structure databases
SMRi | O54962 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204742, 17 interactors |
IntActi | O54962, 4 interactors |
MINTi | O54962 |
STRINGi | 10090.ENSMUSP00000126202 |
PTM databases
iPTMneti | O54962 |
PhosphoSitePlusi | O54962 |
Proteomic databases
EPDi | O54962 |
jPOSTi | O54962 |
MaxQBi | O54962 |
PaxDbi | O54962 |
PeptideAtlasi | O54962 |
PRIDEi | O54962 |
ProteomicsDBi | 273534 |
TopDownProteomicsi | O54962 |
Genome annotation databases
Ensembli | ENSMUST00000025762; ENSMUSP00000025762; ENSMUSG00000024844 ENSMUST00000170010; ENSMUSP00000126202; ENSMUSG00000024844 ENSMUST00000237167; ENSMUSP00000158117; ENSMUSG00000024844 |
GeneIDi | 23825 |
KEGGi | mmu:23825 |
UCSCi | uc008gcr.2, mouse |
Organism-specific databases
CTDi | 8815 |
MGIi | MGI:1346330, Banf1 |
Phylogenomic databases
eggNOGi | KOG4233, Eukaryota |
GeneTreei | ENSGT00390000018613 |
HOGENOMi | CLU_167806_0_0_1 |
InParanoidi | O54962 |
OMAi | TDWCEEF |
OrthoDBi | 1617480at2759 |
PhylomeDBi | O54962 |
TreeFami | TF315060 |
Enzyme and pathway databases
Reactomei | R-MMU-2980766, Nuclear Envelope Breakdown R-MMU-2995383, Initiation of Nuclear Envelope (NE) Reformation |
Miscellaneous databases
BioGRID-ORCSi | 23825, 21 hits in 46 CRISPR screens |
ChiTaRSi | Banf1, mouse |
PROi | PR:O54962 |
RNActi | O54962, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024844, Expressed in liver and 303 other tissues |
Genevisiblei | O54962, MM |
Family and domain databases
Gene3Di | 1.10.150.40, 1 hit |
InterProi | View protein in InterPro IPR004122, BAF_prot IPR036617, BAF_sf |
PANTHERi | PTHR12912, PTHR12912, 1 hit |
Pfami | View protein in Pfam PF02961, BAF, 1 hit |
SMARTi | View protein in SMART SM01023, BAF, 1 hit |
SUPFAMi | SSF47798, SSF47798, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BAF_MOUSE | |
Accessioni | O54962Primary (citable) accession number: O54962 Secondary accession number(s): Q542E6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | June 1, 1998 | |
Last modified: | April 7, 2021 | |
This is version 152 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families