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UniProtKB - O54956 (DPOE2_MOUSE)
Protein
DNA polymerase epsilon subunit 2
Gene
Pole2
Organism
Mus musculus (Mouse)
Status
Functioni
Accessory component of the DNA polymerase epsilon complex (By similarity).
Participates in DNA repair and in chromosomal DNA replication (By similarity).
By similarityMiscellaneous
In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
GO - Biological processi
- DNA-dependent DNA replication Source: MGI
- error-prone translesion synthesis Source: GO_Central
Keywordsi
Molecular function | DNA-binding |
Biological process | DNA replication |
Enzyme and pathway databases
Reactomei | R-MMU-110314, Recognition of DNA damage by PCNA-containing replication complex R-MMU-5651801, PCNA-Dependent Long Patch Base Excision Repair R-MMU-5656169, Termination of translesion DNA synthesis R-MMU-5685942, HDR through Homologous Recombination (HRR) R-MMU-5696397, Gap-filling DNA repair synthesis and ligation in GG-NER R-MMU-5696400, Dual Incision in GG-NER R-MMU-6782135, Dual incision in TC-NER R-MMU-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-MMU-68952, DNA replication initiation R-MMU-68962, Activation of the pre-replicative complex |
Names & Taxonomyi
Protein namesi | Recommended name: DNA polymerase epsilon subunit 2Alternative name(s): DNA polymerase II subunit 2 DNA polymerase epsilon subunit B |
Gene namesi | Name:Pole2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1197514, Pole2 |
VEuPathDBi | HostDB:ENSMUSG00000020974 |
Subcellular locationi
Nucleus
Nucleus
- epsilon DNA polymerase complex Source: UniProtKB
- nuclear body Source: MGI
- nucleoplasm Source: MGI
Other locations
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000071563 | 1 – 527 | DNA polymerase epsilon subunit 2Add BLAST | 527 |
Proteomic databases
EPDi | O54956 |
MaxQBi | O54956 |
PaxDbi | O54956 |
PeptideAtlasi | O54956 |
PRIDEi | O54956 |
ProteomicsDBi | 279568 |
PTM databases
PhosphoSitePlusi | O54956 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000020974, Expressed in ear and 243 other tissues |
ExpressionAtlasi | O54956, baseline and differential |
Genevisiblei | O54956, MM |
Interactioni
Subunit structurei
Component of the DNA polymerase epsilon complex consisting of four subunits: the catalytic subunit POLE and the accessory subunits POLE2, POLE3 and POLE4.
By similarityProtein-protein interaction databases
BioGRIDi | 202293, 5 interactors |
ComplexPortali | CPX-2109, DNA polymerase epsilon complex |
CORUMi | O54956 |
STRINGi | 10090.ENSMUSP00000021359 |
Miscellaneous databases
RNActi | O54956, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the DNA polymerase epsilon subunit B family.Curated
Phylogenomic databases
eggNOGi | KOG3818, Eukaryota |
GeneTreei | ENSGT00390000012435 |
HOGENOMi | CLU_010628_2_0_1 |
InParanoidi | O54956 |
OMAi | CRLQYCT |
OrthoDBi | 375960at2759 |
PhylomeDBi | O54956 |
TreeFami | TF103007 |
Family and domain databases
InterProi | View protein in InterPro IPR007185, DNA_pol_a/d/e_bsu IPR024639, DNA_pol_e_bsu_N IPR016266, POLE2 |
PANTHERi | PTHR12708, PTHR12708, 1 hit |
Pfami | View protein in Pfam PF04042, DNA_pol_E_B, 1 hit PF12213, Dpoe2NT, 1 hit |
PIRSFi | PIRSF000799, DNA_pol_eps_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O54956-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAPERLRSRT VSAFKLRGLL LRSEATKYLT EALQSVTELE LEENLGKIID
60 70 80 90 100
AVEKQPLSSN MIERSVVEAA VQECSQSMDE TIEHVFNIIG AFDIPRFIYN
110 120 130 140 150
SERKKFLPLS MTNHAAPTLF GTARDKAELY LERYTILHQR THRHELFTPP
160 170 180 190 200
VIGSHLEESG SKFQLKTIET LLGSTSKVGD VIVLGMITQL KEGKFFLEDP
210 220 230 240 250
TGTVQLDLSK AQFHSGLYTE ACFVLAEGWF EDQVFHANAF GFPPTEPSST
260 270 280 290 300
TRAYYGNINF FGGPSNASVK TYTKLRQLED ENKDAMFVIV SDVWLDQVQV
310 320 330 340 350
LEKFHVMFSG YSPAPPTCFI LCGNFSSAPY GKNQVQALKD SLKSLADIIC
360 370 380 390 400
EYPSIHQSSR FVFVPGPEDP GFGSILPRPP LAESITQEFR QRVPFSVFTT
410 420 430 440 450
NPCRIQYCTQ EIIIFREDLV NKMCRNCVRF PSSSLDIPTH FVKTILSQGH
460 470 480 490 500
LAPLPLYVCP VHWAYDYTLR VYPVPDLLVI ADKYDPFTVT NTDCLCINPG
510 520
SFPRSGFAFK VFYPSSKTVE DSKLQGF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1Y7VN66 | A0A1Y7VN66_MOUSE | DNA polymerase epsilon subunit | Pole2 | 502 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 78 | M → V in AAH63772 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK077592 mRNA Translation: BAC36883.1 BC063772 mRNA Translation: AAH63772.1 AF036898 mRNA Translation: AAC40045.1 |
CCDSi | CCDS36463.1 |
RefSeqi | NP_035263.1, NM_011133.2 |
Genome annotation databases
Ensembli | ENSMUST00000021359; ENSMUSP00000021359; ENSMUSG00000020974 |
GeneIDi | 18974 |
KEGGi | mmu:18974 |
UCSCi | uc007nrv.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK077592 mRNA Translation: BAC36883.1 BC063772 mRNA Translation: AAH63772.1 AF036898 mRNA Translation: AAC40045.1 |
CCDSi | CCDS36463.1 |
RefSeqi | NP_035263.1, NM_011133.2 |
3D structure databases
AlphaFoldDBi | O54956 |
SMRi | O54956 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 202293, 5 interactors |
ComplexPortali | CPX-2109, DNA polymerase epsilon complex |
CORUMi | O54956 |
STRINGi | 10090.ENSMUSP00000021359 |
PTM databases
PhosphoSitePlusi | O54956 |
Proteomic databases
EPDi | O54956 |
MaxQBi | O54956 |
PaxDbi | O54956 |
PeptideAtlasi | O54956 |
PRIDEi | O54956 |
ProteomicsDBi | 279568 |
Protocols and materials databases
Antibodypediai | 23530, 133 antibodies from 23 providers |
DNASUi | 18974 |
Genome annotation databases
Ensembli | ENSMUST00000021359; ENSMUSP00000021359; ENSMUSG00000020974 |
GeneIDi | 18974 |
KEGGi | mmu:18974 |
UCSCi | uc007nrv.1, mouse |
Organism-specific databases
CTDi | 5427 |
MGIi | MGI:1197514, Pole2 |
VEuPathDBi | HostDB:ENSMUSG00000020974 |
Phylogenomic databases
eggNOGi | KOG3818, Eukaryota |
GeneTreei | ENSGT00390000012435 |
HOGENOMi | CLU_010628_2_0_1 |
InParanoidi | O54956 |
OMAi | CRLQYCT |
OrthoDBi | 375960at2759 |
PhylomeDBi | O54956 |
TreeFami | TF103007 |
Enzyme and pathway databases
Reactomei | R-MMU-110314, Recognition of DNA damage by PCNA-containing replication complex R-MMU-5651801, PCNA-Dependent Long Patch Base Excision Repair R-MMU-5656169, Termination of translesion DNA synthesis R-MMU-5685942, HDR through Homologous Recombination (HRR) R-MMU-5696397, Gap-filling DNA repair synthesis and ligation in GG-NER R-MMU-5696400, Dual Incision in GG-NER R-MMU-6782135, Dual incision in TC-NER R-MMU-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-MMU-68952, DNA replication initiation R-MMU-68962, Activation of the pre-replicative complex |
Miscellaneous databases
BioGRID-ORCSi | 18974, 55 hits in 108 CRISPR screens |
ChiTaRSi | Pole2, mouse |
PROi | PR:O54956 |
RNActi | O54956, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020974, Expressed in ear and 243 other tissues |
ExpressionAtlasi | O54956, baseline and differential |
Genevisiblei | O54956, MM |
Family and domain databases
InterProi | View protein in InterPro IPR007185, DNA_pol_a/d/e_bsu IPR024639, DNA_pol_e_bsu_N IPR016266, POLE2 |
PANTHERi | PTHR12708, PTHR12708, 1 hit |
Pfami | View protein in Pfam PF04042, DNA_pol_E_B, 1 hit PF12213, Dpoe2NT, 1 hit |
PIRSFi | PIRSF000799, DNA_pol_eps_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DPOE2_MOUSE | |
Accessioni | O54956Primary (citable) accession number: O54956 Secondary accession number(s): Q6P3Y7, Q8BP72 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | August 30, 2005 | |
Last modified: | May 25, 2022 | |
This is version 137 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families