Pole2

Functionⁱ

Participates in DNA repair and in chromosomal DNA replication.

Miscellaneous

In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.

Catalytic activityⁱ

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functionⁱ

DNA binding Source: UniProtKB-KW
DNA-directed DNA polymerase activity Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

DNA-dependent DNA replication Source: GO_Central
error-prone translesion synthesis Source: GO_Central

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological process	DNA replication

Enzyme and pathway databases

Reactomeⁱ	R-MMU-110314 Recognition of DNA damage by PCNA-containing replication complex R-MMU-174430 Telomere C-strand synthesis initiation R-MMU-5651801 PCNA-Dependent Long Patch Base Excision Repair R-MMU-5656169 Termination of translesion DNA synthesis R-MMU-5685942 HDR through Homologous Recombination (HRR) R-MMU-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER R-MMU-5696400 Dual Incision in GG-NER R-MMU-6782135 Dual incision in TC-NER R-MMU-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER R-MMU-68952 DNA replication initiation R-MMU-68962 Activation of the pre-replicative complex

Reactomeⁱ

R-MMU-110314 Recognition of DNA damage by PCNA-containing replication complex
R-MMU-174430 Telomere C-strand synthesis initiation
R-MMU-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-MMU-5656169 Termination of translesion DNA synthesis
R-MMU-5685942 HDR through Homologous Recombination (HRR)
R-MMU-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER
R-MMU-5696400 Dual Incision in GG-NER
R-MMU-6782135 Dual incision in TC-NER
R-MMU-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-MMU-68952 DNA replication initiation
R-MMU-68962 Activation of the pre-replicative complex

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: DNA polymerase epsilon subunit 2 (EC:2.7.7.7) Alternative name(s): DNA polymerase II subunit 2 DNA polymerase epsilon subunit B
Gene namesⁱ	Name:Pole2
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 12

Organism-specific databases

MGIⁱ	MGI:1197514 Pole2

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000071563	1 – 527	DNA polymerase epsilon subunit 2Add BLAST		527

Proteomic databases

EPDⁱ	O54956
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O54956
PaxDbⁱ	O54956
PeptideAtlas More...PeptideAtlasⁱ	O54956
PRIDEⁱ	O54956

PTM databases

PhosphoSitePlusⁱ	O54956

PhosphoSitePlusⁱ

O54956

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000020974 Expressed in 217 organ(s), highest expression level in ear
ExpressionAtlasⁱ	O54956 baseline and differential
Genevisibleⁱ	O54956 MM

Interactionⁱ

Subunit structureⁱ

Component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4.

Protein-protein interaction databases

ComplexPortalⁱ	CPX-2109 epsilon DNA polymerase complex
CORUMⁱ	O54956
STRINGⁱ	10090.ENSMUSP00000021359

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O54956
SMRⁱ	O54956
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the DNA polymerase epsilon subunit B family.Curated

Phylogenomic databases

eggNOGⁱ	KOG3818 Eukaryota ENOG410XP7N LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000012435
HOGENOMⁱ	HOG000265254
HOVERGENⁱ	HBG061290
InParanoidⁱ	O54956
KEGG Orthology (KO) More...KOⁱ	K02325
OMAⁱ	PNHFVKT
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G05Q6
PhylomeDBⁱ	O54956
TreeFamⁱ	TF103007

Family and domain databases

InterProⁱ	View protein in InterPro IPR007185 DNA_pol_alpha/epsilon_bsu IPR024639 DNA_pol_e_bsu_N IPR016266 POLE2
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF04042 DNA_pol_E_B, 1 hit PF12213 Dpoe2NT, 1 hit
PIRSFⁱ	PIRSF000799 DNA_pol_eps_2, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

O54956-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAPERLRSRT VSAFKLRGLL LRSEATKYLT EALQSVTELE LEENLGKIID 
        60         70         80         90        100
AVEKQPLSSN MIERSVVEAA VQECSQSMDE TIEHVFNIIG AFDIPRFIYN 
       110        120        130        140        150
SERKKFLPLS MTNHAAPTLF GTARDKAELY LERYTILHQR THRHELFTPP 
       160        170        180        190        200
VIGSHLEESG SKFQLKTIET LLGSTSKVGD VIVLGMITQL KEGKFFLEDP 
       210        220        230        240        250
TGTVQLDLSK AQFHSGLYTE ACFVLAEGWF EDQVFHANAF GFPPTEPSST 
       260        270        280        290        300
TRAYYGNINF FGGPSNASVK TYTKLRQLED ENKDAMFVIV SDVWLDQVQV 
       310        320        330        340        350
LEKFHVMFSG YSPAPPTCFI LCGNFSSAPY GKNQVQALKD SLKSLADIIC 
       360        370        380        390        400
EYPSIHQSSR FVFVPGPEDP GFGSILPRPP LAESITQEFR QRVPFSVFTT 
       410        420        430        440        450
NPCRIQYCTQ EIIIFREDLV NKMCRNCVRF PSSSLDIPTH FVKTILSQGH 
       460        470        480        490        500
LAPLPLYVCP VHWAYDYTLR VYPVPDLLVI ADKYDPFTVT NTDCLCINPG 
       510        520 
SFPRSGFAFK VFYPSSKTVE DSKLQGF

Length:527

Mass (Da):59,387

Last modified:August 30, 2005 - v2

Checksum:ⁱ82940B0FD1584350

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	A0A1Y7VN66	A0A1Y7VN66_MOUSE		DNA polymerase epsilon subunit DNA polymerase epsilon subunit, EC 2.7.7.7 (DNA polymerase II subunit 2)	Pole2	502	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	78	M → V in AAH63772 (PubMed:15489334).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK077592 mRNA Translation: BAC36883.1 BC063772 mRNA Translation: AAH63772.1 AF036898 mRNA Translation: AAC40045.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS36463.1
NCBI Reference Sequences More...RefSeqⁱ	NP_035263.1, NM_011133.2
UniGeneⁱ	Mm.9199

Genome annotation databases

Ensemblⁱ	ENSMUST00000021359; ENSMUSP00000021359; ENSMUSG00000020974
GeneIDⁱ	18974
KEGGⁱ	mmu:18974
UCSC genome browser More...UCSCⁱ	uc007nrv.1 mouse

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O54956	DNA polymerase epsilon subunit		MOUSE		527	UniRef90_O54956
DNA polymerase epsilon subunit		RAT		527
UPI000454B5B4		CRIGR		636
UPI00038BCCBB		MICOH		527
UPI00042AD686		PERMB		527
+11

Protein	Similar proteins		Species	Score	Length	Source
O54956	DNA polymerase epsilon subunit 2	)	HUMAN		527	UniRef50_P56282
DNA polymerase epsilon subunit 2		BOVIN		527
DNA polymerase epsilon subunit		MUSPF		539
DNA polymerase epsilon subunit		PTEAL		535
DNA polymerase epsilon subunit		wild yak		533
+228

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK077592 mRNA Translation: BAC36883.1 BC063772 mRNA Translation: AAH63772.1 AF036898 mRNA Translation: AAC40045.1
CCDSⁱ	CCDS36463.1
RefSeqⁱ	NP_035263.1, NM_011133.2
UniGeneⁱ	Mm.9199

3D structure databases

ProteinModelPortalⁱ	O54956
SMRⁱ	O54956
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

ComplexPortalⁱ	CPX-2109 epsilon DNA polymerase complex
CORUMⁱ	O54956
STRINGⁱ	10090.ENSMUSP00000021359

PTM databases

PhosphoSitePlusⁱ	O54956

PhosphoSitePlusⁱ

O54956

Proteomic databases

EPDⁱ	O54956
MaxQBⁱ	O54956
PaxDbⁱ	O54956
PeptideAtlasⁱ	O54956
PRIDEⁱ	O54956

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000021359; ENSMUSP00000021359; ENSMUSG00000020974
GeneIDⁱ	18974
KEGGⁱ	mmu:18974
UCSCⁱ	uc007nrv.1 mouse

Organism-specific databases

CTDⁱ	5427
MGIⁱ	MGI:1197514 Pole2

Phylogenomic databases

eggNOGⁱ	KOG3818 Eukaryota ENOG410XP7N LUCA
GeneTreeⁱ	ENSGT00390000012435
HOGENOMⁱ	HOG000265254
HOVERGENⁱ	HBG061290
InParanoidⁱ	O54956
KOⁱ	K02325
OMAⁱ	PNHFVKT
OrthoDBⁱ	EOG091G05Q6
PhylomeDBⁱ	O54956
TreeFamⁱ	TF103007

Enzyme and pathway databases

Reactomeⁱ	R-MMU-110314 Recognition of DNA damage by PCNA-containing replication complex R-MMU-174430 Telomere C-strand synthesis initiation R-MMU-5651801 PCNA-Dependent Long Patch Base Excision Repair R-MMU-5656169 Termination of translesion DNA synthesis R-MMU-5685942 HDR through Homologous Recombination (HRR) R-MMU-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER R-MMU-5696400 Dual Incision in GG-NER R-MMU-6782135 Dual incision in TC-NER R-MMU-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER R-MMU-68952 DNA replication initiation R-MMU-68962 Activation of the pre-replicative complex

Reactomeⁱ

R-MMU-110314 Recognition of DNA damage by PCNA-containing replication complex
R-MMU-174430 Telomere C-strand synthesis initiation
R-MMU-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-MMU-5656169 Termination of translesion DNA synthesis
R-MMU-5685942 HDR through Homologous Recombination (HRR)
R-MMU-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER
R-MMU-5696400 Dual Incision in GG-NER
R-MMU-6782135 Dual incision in TC-NER
R-MMU-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-MMU-68952 DNA replication initiation
R-MMU-68962 Activation of the pre-replicative complex

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:O54956
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000020974 Expressed in 217 organ(s), highest expression level in ear
ExpressionAtlasⁱ	O54956 baseline and differential
Genevisibleⁱ	O54956 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR007185 DNA_pol_alpha/epsilon_bsu IPR024639 DNA_pol_e_bsu_N IPR016266 POLE2
Pfamⁱ	View protein in Pfam PF04042 DNA_pol_E_B, 1 hit PF12213 Dpoe2NT, 1 hit
PIRSFⁱ	PIRSF000799 DNA_pol_eps_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	DPOE2_MOUSE
Accessionⁱ	O54956Primary (citable) accession number: O54956 Secondary accession number(s): Q6P3Y7, Q8BP72
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
	Last sequence update:	August 30, 2005
	Last modified:	September 12, 2018
	This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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Supporting data

UniProtKB - O54956 (DPOE2_MOUSE)

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DNA polymerase epsilon subunit 2

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ