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Protein

DnaJ homolog subfamily B member 6

Gene

Dnajb6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins (By similarity). Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily B member 6
Alternative name(s):
Heat shock protein J2
Short name:
HSJ-2
MRJ
mDj4
Gene namesi
Name:Dnajb6
Synonyms:Hsj2, Mrj
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1344381 Dnajb6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000710261 – 365DnaJ homolog subfamily B member 6Add BLAST365

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei136Omega-N-methylarginineCombined sources1

Keywords - PTMi

Methylation

Proteomic databases

EPDiO54946
MaxQBiO54946
PaxDbiO54946
PeptideAtlasiO54946
PRIDEiO54946

PTM databases

iPTMnetiO54946
PhosphoSitePlusiO54946
SwissPalmiO54946

Expressioni

Gene expression databases

BgeeiENSMUSG00000029131 Expressed in 186 organ(s), highest expression level in testis
CleanExiMM_DNAJB6
ExpressionAtlasiO54946 baseline and differential
GenevisibleiO54946 MM

Interactioni

Subunit structurei

Homooligomer (By similarity). Interacts with BAG3, HSPB8 and STUB1 (By similarity). Interacts with HSP70, KRT18 and PTTG. Isoform B interacts with histone deacetylases HDAC4, HDAC6, and SIRT2, HDAC activity is required for antiaggregation (By similarity). Interacts with ALKBH1.By similarity1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204812, 9 interactors
IntActiO54946, 7 interactors
MINTiO54946
STRINGi10090.ENSMUSP00000008733

Structurei

3D structure databases

ProteinModelPortaliO54946
SMRiO54946
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 69JPROSITE-ProRule annotationAdd BLAST67

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 147Interaction with HSP70By similarityAdd BLAST147
Regioni120 – 235Interaction with KRT18By similarityAdd BLAST116

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi75 – 81Poly-Gly7
Compositional biasi83 – 172Gly/Phe-richAdd BLAST90
Compositional biasi84 – 165Phe-richAdd BLAST82
Compositional biasi158 – 194Ser-richAdd BLAST37
Compositional biasi249 – 307Pro-richAdd BLAST59
Compositional biasi356 – 359Poly-Lys4

Domaini

The antiaggregation activity of resides in the serine-rich region and the C-terminus.By similarity

Phylogenomic databases

eggNOGiKOG0714 Eukaryota
COG0484 LUCA
GeneTreeiENSGT00760000118947
HOVERGENiHBG066998
InParanoidiO54946
KOiK09512
OMAiFFGVNSP
OrthoDBiEOG091G152F
PhylomeDBiO54946
TreeFamiTF105142

Family and domain databases

CDDicd06257 DnaJ, 1 hit
Gene3Di1.10.287.110, 1 hit
InterProiView protein in InterPro
IPR001623 DnaJ_domain
IPR018253 DnaJ_domain_CS
IPR036869 J_dom_sf
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
PROSITEiView protein in PROSITE
PS00636 DNAJ_1, 1 hit
PS50076 DNAJ_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform A (identifier: O54946-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MVDYYEVLGV QRHASPEDIK KAYRKQALKW HPDKNPENKE EAERKFKQVA
60 70 80 90 100
EAYEVLSDAK KRDIYDKYGK EGLNGGGGGG GIHFDSPFEF GFTFRNPDDV
110 120 130 140 150
FREFFGGRDP FSFDFFEDPF DDFFGNRRGP RGNRSRGAGS FFSTFSGFPS
160 170 180 190 200
FGSGFPAFDT GFTPFGSLGH GGLTSFSSTS FGGSGMGNFK SISTSTKIVN
210 220 230 240 250
GKKITTKRIV ENGQERVEVE EDGQLKSLTI NGVADENALA EECQRRGQPT
260 270 280 290 300
PALAPGPAPA PVRVPSQARP LAPTPAPTPA PTPAPAPAQT PAPSVSTRPQ
310 320 330 340 350
KPPRPAPTAK LGSKSNWEDD EQDRQRVPGN WDAPMTSAGL KEGGKRKKQK
360
QKEDLKKKKS TKGNH
Note: No experimental confirmation available.
Length:365
Mass (Da):39,807
Last modified:June 26, 2007 - v4
Checksum:i7B0102218E8DAA99
GO
Isoform B (identifier: O54946-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     233-242: VADENALAEE → KEHLLRLDNK
     243-365: Missing.

Show »
Length:242
Mass (Da):26,978
Checksum:i3B56053C3BB0735D
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3X8S5G3X8S5_MOUSE
DnaJ homolog subfamily B member 6
Dnajb6 mCG_11633
261Annotation score:
A0A0G2JER9A0A0G2JER9_MOUSE
DnaJ homolog subfamily B member 6
Dnajb6
243Annotation score:
A0A0G2JEI3A0A0G2JEI3_MOUSE
DnaJ homolog subfamily B member 6
Dnajb6
62Annotation score:
F6YRQ2F6YRQ2_MOUSE
DnaJ homolog subfamily B member 6
Dnajb6
231Annotation score:
A0A0G2JGN9A0A0G2JGN9_MOUSE
DnaJ homolog subfamily B member 6
Dnajb6
22Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti117Missing in BAE41354 (PubMed:16141072).Curated1
Sequence conflicti139 – 140GS → AP in AAC16759 (Ref. 2) Curated2
Sequence conflicti188 – 189Missing in BAE25752 (PubMed:16141072).Curated2
Sequence conflicti218E → V in AAH03702 (PubMed:15489334).Curated1
Sequence conflicti227S → P in AAC16759 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_026212233 – 242VADENALAEE → KEHLLRLDNK in isoform B. 4 Publications10
Alternative sequenceiVSP_026213243 – 365Missing in isoform B. 4 PublicationsAdd BLAST123

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028854 mRNA Translation: BAA88302.1
AF035962 mRNA Translation: AAC16759.1
AK133805 mRNA Translation: BAE21853.1
AK134387 mRNA Translation: BAE22124.1
AK144182 mRNA Translation: BAE25752.1
AK151219 mRNA Translation: BAE30213.1
AK151752 mRNA Translation: BAE30661.1
AK152626 mRNA Translation: BAE31369.1
AK152632 mRNA Translation: BAE31375.1
AK153091 mRNA Translation: BAE31714.1
AK169332 mRNA Translation: BAE41085.1
AK169767 mRNA Translation: BAE41354.1
BC003702 mRNA Translation: AAH03702.1
BC083349 mRNA Translation: AAH83349.1
CCDSiCCDS19151.1 [O54946-1]
CCDS39043.1 [O54946-2]
RefSeqiNP_001033029.1, NM_001037940.4 [O54946-1]
NP_001120839.1, NM_001127367.1
NP_035977.2, NM_011847.4 [O54946-2]
UniGeneiMm.290110

Genome annotation databases

EnsembliENSMUST00000008733; ENSMUSP00000008733; ENSMUSG00000029131 [O54946-1]
ENSMUST00000114839; ENSMUSP00000110488; ENSMUSG00000029131 [O54946-2]
GeneIDi23950
KEGGimmu:23950
UCSCiuc008wup.2 mouse [O54946-2]
uc008wur.2 mouse [O54946-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028854 mRNA Translation: BAA88302.1
AF035962 mRNA Translation: AAC16759.1
AK133805 mRNA Translation: BAE21853.1
AK134387 mRNA Translation: BAE22124.1
AK144182 mRNA Translation: BAE25752.1
AK151219 mRNA Translation: BAE30213.1
AK151752 mRNA Translation: BAE30661.1
AK152626 mRNA Translation: BAE31369.1
AK152632 mRNA Translation: BAE31375.1
AK153091 mRNA Translation: BAE31714.1
AK169332 mRNA Translation: BAE41085.1
AK169767 mRNA Translation: BAE41354.1
BC003702 mRNA Translation: AAH03702.1
BC083349 mRNA Translation: AAH83349.1
CCDSiCCDS19151.1 [O54946-1]
CCDS39043.1 [O54946-2]
RefSeqiNP_001033029.1, NM_001037940.4 [O54946-1]
NP_001120839.1, NM_001127367.1
NP_035977.2, NM_011847.4 [O54946-2]
UniGeneiMm.290110

3D structure databases

ProteinModelPortaliO54946
SMRiO54946
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204812, 9 interactors
IntActiO54946, 7 interactors
MINTiO54946
STRINGi10090.ENSMUSP00000008733

PTM databases

iPTMnetiO54946
PhosphoSitePlusiO54946
SwissPalmiO54946

Proteomic databases

EPDiO54946
MaxQBiO54946
PaxDbiO54946
PeptideAtlasiO54946
PRIDEiO54946

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000008733; ENSMUSP00000008733; ENSMUSG00000029131 [O54946-1]
ENSMUST00000114839; ENSMUSP00000110488; ENSMUSG00000029131 [O54946-2]
GeneIDi23950
KEGGimmu:23950
UCSCiuc008wup.2 mouse [O54946-2]
uc008wur.2 mouse [O54946-1]

Organism-specific databases

CTDi10049
MGIiMGI:1344381 Dnajb6

Phylogenomic databases

eggNOGiKOG0714 Eukaryota
COG0484 LUCA
GeneTreeiENSGT00760000118947
HOVERGENiHBG066998
InParanoidiO54946
KOiK09512
OMAiFFGVNSP
OrthoDBiEOG091G152F
PhylomeDBiO54946
TreeFamiTF105142

Miscellaneous databases

ChiTaRSiDnajb6 mouse
PROiPR:O54946
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029131 Expressed in 186 organ(s), highest expression level in testis
CleanExiMM_DNAJB6
ExpressionAtlasiO54946 baseline and differential
GenevisibleiO54946 MM

Family and domain databases

CDDicd06257 DnaJ, 1 hit
Gene3Di1.10.287.110, 1 hit
InterProiView protein in InterPro
IPR001623 DnaJ_domain
IPR018253 DnaJ_domain_CS
IPR036869 J_dom_sf
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
PROSITEiView protein in PROSITE
PS00636 DNAJ_1, 1 hit
PS50076 DNAJ_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDNJB6_MOUSE
AccessioniPrimary (citable) accession number: O54946
Secondary accession number(s): Q3TE94
, Q3U6L0, Q3UNJ5, Q3UYT7, Q99LA5, Q9QYI9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 26, 2007
Last modified: September 12, 2018
This is version 157 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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