UniProtKB - O54939 (DHB3_RAT)
17-beta-hydroxysteroid dehydrogenase type 3
Hsd17b3
Functioni
Catalyzes the conversion of 17-oxosteroids to 17beta-hydroxysteroids. Favors the reduction of androstenedione to testosterone. Testosterone is the key androgen driving male development and function (By similarity).
Uses NADPH while the two other EDH17B enzymes use NADH. Androgens such as epiandrosterone, dehydroepiandrosterone, androsterone and androstanedione are accepted as substrates and reduced at C-17. Can reduce 11-ketoandrostenedione as well as 11beta-hydroxyandrostenedione at C-17 to the respective testosterone forms (By similarity).
Plays a role in the rate-limiting-step for the maximum level of testosterone production by the testis but does not affect basal testosterone production (By similarity).
By similarityCatalytic activityi
- This reaction proceeds in the backwardBy similarity direction.
- EC:1.1.1.64By similarityThis reaction proceeds in the backwardBy similarity direction.
- EC:1.1.1.62By similarityThis reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- androst-4-ene-3,11,17-trione + H+ + NADPH = 17β-hydroxyandrost-4-ene-3,11-dione + NADP+By similarityThis reaction proceeds in the forwardBy similarity direction.
- 11β-hydroxyandrost-4-ene-3,17-dione + H+ + NADPH = 11β,17β-dihydroxyandrost-4-ene-3-one + NADP+By similarityThis reaction proceeds in the forwardBy similarity direction.
: testosterone biosynthesis Pathwayi
This protein is involved in the pathway testosterone biosynthesis, which is part of Hormone biosynthesis.By similarityView all proteins of this organism that are known to be involved in the pathway testosterone biosynthesis and in Hormone biosynthesis.
Pathwayi: Steroid metabolism
This protein is involved in Steroid metabolism.By similarityView all proteins of this organism that are known to be involved in Steroid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 181 | SubstrateBy similarity | 1 | |
Active sitei | 194 | Proton acceptorBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 44 – 73 | NADPBy similarityAdd BLAST | 30 |
GO - Molecular functioni
- 17-beta-hydroxysteroid dehydrogenase (NADP+) activity Source: RHEA
- estradiol 17-beta-dehydrogenase activity Source: RGD
- oxidoreductase activity Source: GO_Central
- testosterone 17-beta-dehydrogenase (NADP+) activity Source: UniProtKB
- testosterone dehydrogenase (NAD+) activity Source: RGD
GO - Biological processi
- biphenyl metabolic process Source: RGD
- cellular response to antibiotic Source: RGD
- cellular response to gonadotropin stimulus Source: RGD
- cellular response to hormone stimulus Source: RGD
- hippocampus development Source: RGD
- insecticide metabolic process Source: RGD
- Leydig cell differentiation Source: RGD
- male genitalia development Source: InterPro
- organic acid metabolic process Source: RGD
- phenol-containing compound metabolic process Source: RGD
- response to activity Source: RGD
- response to arsenic-containing substance Source: RGD
- response to cadmium ion Source: RGD
- response to corticosterone Source: RGD
- response to estrogen Source: RGD
- response to ethanol Source: RGD
- response to fatty acid Source: RGD
- response to fungicide Source: RGD
- response to herbicide Source: RGD
- response to insecticide Source: RGD
- response to L-ascorbic acid Source: RGD
- response to lead ion Source: RGD
- response to metal ion Source: RGD
- response to nutrient levels Source: RGD
- response to organic cyclic compound Source: RGD
- response to steroid hormone Source: RGD
- response to vitamin E Source: RGD
- steroid biosynthetic process Source: RGD
- testosterone biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis |
Ligand | NADP |
Enzyme and pathway databases
BRENDAi | 1.1.1.51, 5301 1.1.1.64, 5301 |
Reactomei | R-RNO-193048, Androgen biosynthesis R-RNO-75876, Synthesis of very long-chain fatty acyl-CoAs |
UniPathwayi | UPA00367 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621805, Hsd17b3 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
Other locations
- intracellular membrane-bounded organelle Source: RGD
Keywords - Cellular componenti
Endoplasmic reticulumPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000054575 | 1 – 306 | 17-beta-hydroxysteroid dehydrogenase type 3Add BLAST | 306 |
Proteomic databases
PaxDbi | O54939 |
PTM databases
iPTMneti | O54939 |
PhosphoSitePlusi | O54939 |
Interactioni
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000025850 |
Chemistry databases
BindingDBi | O54939 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1014, Eukaryota |
InParanoidi | O54939 |
OrthoDBi | 895581at2759 |
PhylomeDBi | O54939 |
Family and domain databases
InterProi | View protein in InterPro IPR033281, HSD17B3 IPR036291, NAD(P)-bd_dom_sf IPR002347, SDR_fam |
PANTHERi | PTHR43899:SF7, PTHR43899:SF7, 1 hit |
Pfami | View protein in Pfam PF00106, adh_short, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MEQFLLSVGL LVCLVCLVKC VRFSRYLFLS FCKALPGSFL RSMGQWAVIT
60 70 80 90 100
GAGDGIGKAY SFELARHGLN VVLISRTLEK LQVISEEIER TTGSRVKVVQ
110 120 130 140 150
ADFTREDIYD HIEEQLKGLE IGVLVNNVGM LPNLLPSHFL STSGESQSVI
160 170 180 190 200
HCNITSVVKM TQLVLKHMES RRRGLILNIS SGVGVRPWPL YSLYSASKAF
210 220 230 240 250
VCTFSKALNV EYRDKGIIIQ VLTPYSVSTP MTKYLNTSRV TKTADEFVKE
260 270 280 290 300
SLKYVTIGAE TCGCLAHEIL AIILNLIPSR IFYSSTTQRF LLKQFSDYLK
SNISNR
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF1LMA3 | F1LMA3_RAT | Testosterone 17-beta-dehydrogenase ... | Hsd17b3 | 306 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF035156 mRNA Translation: AAB99739.1 |
RefSeqi | NP_446459.1, NM_054007.1 |
Genome annotation databases
GeneIDi | 117182 |
KEGGi | rno:117182 |
UCSCi | RGD:621805, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF035156 mRNA Translation: AAB99739.1 |
RefSeqi | NP_446459.1, NM_054007.1 |
3D structure databases
SMRi | O54939 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000025850 |
Chemistry databases
BindingDBi | O54939 |
ChEMBLi | CHEMBL1075158 |
DrugCentrali | O54939 |
PTM databases
iPTMneti | O54939 |
PhosphoSitePlusi | O54939 |
Proteomic databases
PaxDbi | O54939 |
Genome annotation databases
GeneIDi | 117182 |
KEGGi | rno:117182 |
UCSCi | RGD:621805, rat |
Organism-specific databases
CTDi | 3293 |
RGDi | 621805, Hsd17b3 |
Phylogenomic databases
eggNOGi | KOG1014, Eukaryota |
InParanoidi | O54939 |
OrthoDBi | 895581at2759 |
PhylomeDBi | O54939 |
Enzyme and pathway databases
UniPathwayi | UPA00367 |
BRENDAi | 1.1.1.51, 5301 1.1.1.64, 5301 |
Reactomei | R-RNO-193048, Androgen biosynthesis R-RNO-75876, Synthesis of very long-chain fatty acyl-CoAs |
Miscellaneous databases
PROi | PR:O54939 |
Family and domain databases
InterProi | View protein in InterPro IPR033281, HSD17B3 IPR036291, NAD(P)-bd_dom_sf IPR002347, SDR_fam |
PANTHERi | PTHR43899:SF7, PTHR43899:SF7, 1 hit |
Pfami | View protein in Pfam PF00106, adh_short, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DHB3_RAT | |
Accessioni | O54939Primary (citable) accession number: O54939 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | June 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families