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Protein

A-kinase anchor protein 2

Gene

Akap2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to regulatory subunit (RII) of protein kinase A. May be involved in establishing polarity in signaling systems or in integrating PKA-RII isoforms with downstream effectors to capture, amplify and focus diffuse, trans-cellular signals carried by cAMP.

GO - Molecular functioni

  • protein domain specific binding Source: MGI
  • protein kinase A binding Source: MGI

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 2
Short name:
AKAP-2
Alternative name(s):
AKAP expressed in kidney and lung
Short name:
AKAP-KL
Protein kinase A-anchoring protein 2
Short name:
PRKA2
Gene namesi
Name:Akap2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1306795 Akap2

Subcellular locationi

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi590A → S: No effect on binding to RII. 1 Publication1
Mutagenesisi598I → A: Reduces binding to RII. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000206571 – 893A-kinase anchor protein 2Add BLAST893

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei122PhosphoserineBy similarity1
Modified residuei152PhosphoserineBy similarity1
Cross-linki174Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki174Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei357PhosphoserineBy similarity1
Modified residuei482PhosphoserineBy similarity1
Modified residuei486PhosphoserineBy similarity1
Modified residuei538PhosphoserineBy similarity1
Modified residuei547PhosphothreonineBy similarity1
Modified residuei651PhosphoserineBy similarity1
Modified residuei740PhosphoserineBy similarity1
Modified residuei768PhosphoserineBy similarity1
Modified residuei799PhosphoserineBy similarity1
Modified residuei806PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiO54931
PaxDbiO54931
PeptideAtlasiO54931
PRIDEiO54931

PTM databases

iPTMnetiO54931
PhosphoSitePlusiO54931

Expressioni

Tissue specificityi

Highly expressed in lung and weakly in thymus and cerebellum. Little or no expression in liver, heart and cerebral cortex. All isoforms are expressed in lung, but KL2A and KL2B isoforms are the principal isoforms in cerebellum.

Gene expression databases

BgeeiENSMUSG00000038729 Expressed in 33 organ(s), highest expression level in lung
CleanExiMM_AKAP2
ExpressionAtlasiO54931 baseline and differential
GenevisibleiO54931 MM

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Prkar2aP123673EBI-645828,EBI-645747

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198049, 2 interactors
IntActiO54931, 4 interactors
MINTiO54931
STRINGi10090.ENSMUSP00000117466

Structurei

3D structure databases

ProteinModelPortaliO54931
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni586 – 599PKA-RII subunit binding domainAdd BLAST14

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili255 – 316Sequence analysisAdd BLAST62
Coiled coili729 – 766Sequence analysisAdd BLAST38

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi274 – 311Gln-richAdd BLAST38

Domaini

The RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IG9A Eukaryota
ENOG410ZMFZ LUCA
GeneTreeiENSGT00930000151059
HOGENOMiHOG000231745
HOVERGENiHBG050477
InParanoidiO54931
KOiK16519
PhylomeDBiO54931
TreeFamiTF105402

Family and domain databases

InterProiView protein in InterPro
IPR029304 AKAP2_C
PfamiView protein in Pfam
PF15304 AKAP2_C, 1 hit

Sequences (5+)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing and alternative initiation. AlignAdd to basket

This entry has 5 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O54931-1) [UniParc]FASTAAdd to basket
Also known as: KL1A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEIGVSVAEC KSVPGVTSTP HSKDHSSPFY SPSHNGLLAD HHESLDNDVA
60 70 80 90 100
REIQYLDEVL EANCCDSSVD GTYNGISSPE PGAAILVSSL GSPAHSVTEA
110 120 130 140 150
EPTEKASGRQ VPPHIELSRS PSDRMAEGER ANGHSTDQPQ DLLGNSLQAP
160 170 180 190 200
ASPSSSTSSH CSSRDGEFTL TTLKKEAKFE LRAFHEDKKP SKLFEEDERE
210 220 230 240 250
KEQFCVRKVR PSEEMIELEK ERRELIRSQA VKKNPGIAAK WWNPPQEKTI
260 270 280 290 300
EEQLDEEHLE SHRKYKERKE KRAQQEQLQL QQQQQQQLQQ QQLQQQQLQQ
310 320 330 340 350
QQLQQQLQQQ QLSTSQPCTA PAAHKHLDGI EHTKEDVVTE QIDFSAARKQ
360 370 380 390 400
FQLMENSRQT LAKGQSTPRL FSIKPYYKPL GSIHSDKPPT ILRPATVGGT
410 420 430 440 450
LEDGGTQAAK EQKAPCVSES QSAGAGPANA ATQGKEGPYS EPSKRGPLSK
460 470 480 490 500
LWAEDGEFTS ARAVLTVVKD EDHGILDQFS RSVNVSLTQE ELDSGLDELS
510 520 530 540 550
VRSQDTTVLE TLSNDFSMDN ISDSGASNET PSALQENSLA DFSLPQTPQT
560 570 580 590 600
DNPSEGREGV SKSFSDHGFY SPSSTLGDSP SVDDPLEYQA GLLVQNAIQQ
610 620 630 640 650
AIAEQVDKAE AHTSKEGSEQ QEPEATVEEA GSQTPGSEKP QGMFAPPQVS
660 670 680 690 700
SPVQEKRDIL PKNLPAEDRA LREKGPSQPP TAAQPSGPVN MEETRPEGGY
710 720 730 740 750
FSKYSEAAEL RSTASLLATQ ESDVMVGPFK LRSRKQRTLS MIEEEIRAAQ
760 770 780 790 800
EREEELKRQR QVRQSTPSPR AKNAPSLPSR TTCYKTAPGK IEKVKPPPSP
810 820 830 840 850
TTEGPSLQPD LAPEEAAGTQ RPKNLMQTLM EDYETHKSKR RERMDDSSYT
860 870 880 890
SKLLSCKVTS EVLEATRVNR RKSALALRWE AGIYANQEEE DNE
Length:893
Mass (Da):98,579
Last modified:July 28, 2009 - v3
Checksum:i4C9ADD7C4362EF49
GO
Isoform 2 (identifier: O54931-2) [UniParc]FASTAAdd to basket
Also known as: KL2A

The sequence of this isoform differs from the canonical sequence as follows:
     849-861: Missing.

Note: Produced by alternative splicing.
Show »
Length:880
Mass (Da):97,138
Checksum:i1CD1888BA492C55D
GO
Isoform 3 (identifier: O54931-3) [UniParc]FASTAAdd to basket
Also known as: KL3A

The sequence of this isoform differs from the canonical sequence as follows:
     790-795: KIEKVK → PGGHTG
     796-893: Missing.

Note: Produced by alternative splicing.
Show »
Length:795
Mass (Da):87,270
Checksum:i6BB58D5EABDF283A
GO
Isoform 4 (identifier: O54931-4) [UniParc]FASTAAdd to basket
Also known as: KL1B

The sequence of this isoform differs from the canonical sequence as follows:
     1-124: Missing.

Note: Produced by alternative initiation at Met-125 of isoform KL1A.
Show »
Length:769
Mass (Da):85,517
Checksum:i6EF28D2ACAEFDB47
GO
Isoform 5 (identifier: O54931-5) [UniParc]FASTAAdd to basket
Also known as: KL2B

The sequence of this isoform differs from the canonical sequence as follows:
     1-124: Missing.
     849-861: Missing.

Note: Produced by alternative initiation at Met-125 of isoform KL2A.
Show »
Length:756
Mass (Da):84,077
Checksum:iC30DF3CEC4058A6A
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2API8A2API8_MOUSE
A-kinase anchor protein 2
Akap2
921Annotation score:
A2APJ1A2APJ1_MOUSE
A-kinase anchor protein 2
Akap2
176Annotation score:
A2API9A2API9_MOUSE
A-kinase anchor protein 2
Akap2
186Annotation score:

Sequence cautioni

The sequence AAC02206 differs from that shown. Reason: Frameshift at position 875.Curated
The sequence AAC02207 differs from that shown. Reason: Frameshift at position 875.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti120S → I in AAC02206 (PubMed:9497389).Curated1
Sequence conflicti120S → I in AAC02207 (PubMed:9497389).Curated1
Sequence conflicti120S → I in AAC02208 (PubMed:9497389).Curated1
Sequence conflicti264K → R in AAC02206 (PubMed:9497389).Curated1
Sequence conflicti264K → R in AAC02207 (PubMed:9497389).Curated1
Sequence conflicti264K → R in AAC02208 (PubMed:9497389).Curated1
Sequence conflicti531P → T in AAC02206 (PubMed:9497389).Curated1
Sequence conflicti531P → T in AAC02207 (PubMed:9497389).Curated1
Sequence conflicti531P → T in AAC02208 (PubMed:9497389).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0187761 – 124Missing in isoform 4 and isoform 5. CuratedAdd BLAST124
Alternative sequenceiVSP_004097790 – 795KIEKVK → PGGHTG in isoform 3. 1 Publication6
Alternative sequenceiVSP_004098796 – 893Missing in isoform 3. 1 PublicationAdd BLAST98
Alternative sequenceiVSP_004099849 – 861Missing in isoform 2 and isoform 5. 2 PublicationsAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033274 mRNA Translation: AAC02206.1 Frameshift.
AF033275 mRNA Translation: AAC02207.1 Frameshift.
AF033276 mRNA Translation: AAC02208.1
AK077020 mRNA Translation: BAC36571.1
AK147420 mRNA Translation: BAE27901.1
AL840629 Genomic DNA No translation available.
CCDSiCCDS18203.1 [O54931-2]
CCDS18204.1 [O54931-1]
CCDS18205.1 [O54931-3]
PIRiT09225
RefSeqiNP_001030609.1, NM_001035532.2 [O54931-2]
NP_001030610.1, NM_001035533.2 [O54931-1]
NP_001291473.1, NM_001304544.1
UniGeneiMm.331630
Mm.467752

Genome annotation databases

EnsembliENSMUST00000043456; ENSMUSP00000048678; ENSMUSG00000038729 [O54931-1]
ENSMUST00000098064; ENSMUSP00000095672; ENSMUSG00000038729 [O54931-2]
ENSMUST00000102902; ENSMUSP00000099966; ENSMUSG00000038729 [O54931-3]
ENSMUST00000102903; ENSMUSP00000099967; ENSMUSG00000038729 [O54931-3]
ENSMUST00000107598; ENSMUSP00000103224; ENSMUSG00000038729 [O54931-2]
GeneIDi11641
677884
KEGGimmu:11641
mmu:677884
UCSCiuc008syj.2 mouse [O54931-2]
uc008syk.2 mouse [O54931-3]
uc008syl.2 mouse [O54931-1]

Keywords - Coding sequence diversityi

Alternative initiation, Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033274 mRNA Translation: AAC02206.1 Frameshift.
AF033275 mRNA Translation: AAC02207.1 Frameshift.
AF033276 mRNA Translation: AAC02208.1
AK077020 mRNA Translation: BAC36571.1
AK147420 mRNA Translation: BAE27901.1
AL840629 Genomic DNA No translation available.
CCDSiCCDS18203.1 [O54931-2]
CCDS18204.1 [O54931-1]
CCDS18205.1 [O54931-3]
PIRiT09225
RefSeqiNP_001030609.1, NM_001035532.2 [O54931-2]
NP_001030610.1, NM_001035533.2 [O54931-1]
NP_001291473.1, NM_001304544.1
UniGeneiMm.331630
Mm.467752

3D structure databases

ProteinModelPortaliO54931
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198049, 2 interactors
IntActiO54931, 4 interactors
MINTiO54931
STRINGi10090.ENSMUSP00000117466

PTM databases

iPTMnetiO54931
PhosphoSitePlusiO54931

Proteomic databases

MaxQBiO54931
PaxDbiO54931
PeptideAtlasiO54931
PRIDEiO54931

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043456; ENSMUSP00000048678; ENSMUSG00000038729 [O54931-1]
ENSMUST00000098064; ENSMUSP00000095672; ENSMUSG00000038729 [O54931-2]
ENSMUST00000102902; ENSMUSP00000099966; ENSMUSG00000038729 [O54931-3]
ENSMUST00000102903; ENSMUSP00000099967; ENSMUSG00000038729 [O54931-3]
ENSMUST00000107598; ENSMUSP00000103224; ENSMUSG00000038729 [O54931-2]
GeneIDi11641
677884
KEGGimmu:11641
mmu:677884
UCSCiuc008syj.2 mouse [O54931-2]
uc008syk.2 mouse [O54931-3]
uc008syl.2 mouse [O54931-1]

Organism-specific databases

CTDi11217
677884
MGIiMGI:1306795 Akap2

Phylogenomic databases

eggNOGiENOG410IG9A Eukaryota
ENOG410ZMFZ LUCA
GeneTreeiENSGT00930000151059
HOGENOMiHOG000231745
HOVERGENiHBG050477
InParanoidiO54931
KOiK16519
PhylomeDBiO54931
TreeFamiTF105402

Miscellaneous databases

ChiTaRSiAkap2 mouse
PROiPR:O54931
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038729 Expressed in 33 organ(s), highest expression level in lung
CleanExiMM_AKAP2
ExpressionAtlasiO54931 baseline and differential
GenevisibleiO54931 MM

Family and domain databases

InterProiView protein in InterPro
IPR029304 AKAP2_C
PfamiView protein in Pfam
PF15304 AKAP2_C, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAKAP2_MOUSE
AccessioniPrimary (citable) accession number: O54931
Secondary accession number(s): A2APJ4
, O54932, O54933, Q8C5W1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: July 28, 2009
Last modified: November 7, 2018
This is version 149 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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