UniProtKB - O54915 (NR1I2_MOUSE)
Nuclear receptor subfamily 1 group I member 2
Nr1i2
Functioni
Nuclear receptor that binds and is activated by a variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, endogenous compounds and drugs. Response to specific ligands is species-specific, due to differences in the ligand-binding domain. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes (By similarity).
Activated by naturally occurring steroids such as pregnenolone and progesterone, the cholesterol metabolite 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol, synthetic glucocorticoids and antiglucocorticoids and 16-alpha-carbonitrile (PCN).
By similarity2 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 244 | Hyperforin; agonistBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 35 – 104 | Nuclear receptorPROSITE-ProRule annotationAdd BLAST | 70 | |
Zinc fingeri | 38 – 58 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 74 – 99 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 26 |
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- nuclear receptor activity Source: UniProtKB
- nuclear receptor binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
- zinc ion binding Source: InterPro
GO - Biological processi
- cell differentiation Source: GO_Central
- negative regulation of transcription, DNA-templated Source: MGI
- negative regulation of transcription by RNA polymerase II Source: GO_Central
- positive regulation of gene expression Source: MGI
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- xenobiotic catabolic process Source: UniProtKB
- xenobiotic metabolic process Source: UniProtKB
- xenobiotic transport Source: UniProtKB
Keywordsi
Molecular function | Activator, DNA-binding, Receptor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-383280, Nuclear Receptor transcription pathway R-MMU-4090294, SUMOylation of intracellular receptors |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear receptor subfamily 1 group I member 2Alternative name(s): Orphan nuclear receptor PXR Pregnane X receptor |
Gene namesi | Name:Nr1i2 Synonyms:Pxr |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1337040, Nr1i2 |
VEuPathDBi | HostDB:ENSMUSG00000022809 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation
Cytoskeleton
- intermediate filament cytoskeleton Source: MGI
Nucleus
- nuclear body Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- transcription regulator complex Source: Ensembl
Keywords - Cellular componenti
NucleusPathology & Biotechi
Chemistry databases
ChEMBLi | CHEMBL1743244 |
DrugCentrali | O54915 |
GuidetoPHARMACOLOGYi | 606 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000053549 | 1 – 431 | Nuclear receptor subfamily 1 group I member 2Add BLAST | 431 |
Proteomic databases
PaxDbi | O54915 |
PRIDEi | O54915 |
ProteomicsDBi | 293717 [O54915-1] 293718 [O54915-2] |
PTM databases
PhosphoSitePlusi | O54915 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000022809, Expressed in liver and 77 other tissues |
ExpressionAtlasi | O54915, baseline and differential |
Genevisiblei | O54915, MM |
Interactioni
Subunit structurei
Heterodimer with RXRA (By similarity).
Interacts with NCOA1 (By similarity).
Interacts (via domain NR LBD) with CRY1 and CRY2 in a ligand-dependent manner (PubMed:28751364).
By similarity1 PublicationGO - Molecular functioni
- nuclear receptor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 201839, 5 interactors |
ComplexPortali | CPX-505, RXRalpha-PXR nuclear receptor complex CPX-644, PXR-NCOA1 activated nuclear receptor complex |
IntActi | O54915, 2 interactors |
STRINGi | 10090.ENSMUSP00000023504 |
Miscellaneous databases
RNActi | O54915, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 143 – 430 | NR LBDPROSITE-ProRule annotationAdd BLAST | 288 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 105 – 142 | HingeAdd BLAST | 38 | |
Regioni | 282 – 285 | Hyperforin binding; agonistBy similarity | 4 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 63 – 89 | Bipartite nuclear localization signalBy similarityAdd BLAST | 27 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 38 – 58 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 74 – 99 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 26 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG3575, Eukaryota |
GeneTreei | ENSGT00940000161118 |
HOGENOMi | CLU_007368_12_0_1 |
InParanoidi | O54915 |
OMAi | GSIWNYK |
PhylomeDBi | O54915 |
TreeFami | TF316304 |
Family and domain databases
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
InterProi | View protein in InterPro IPR035500, NHR-like_dom_sf IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PRINTSi | PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRPEESWSRV GLVQCEEADS ALEEPINVEE EDGGLQICRV CGDKANGYHF
60 70 80 90 100
NVMTCEGCKG FFRRAMKRNV RLRCPFRKGT CEITRKTRRQ CQACRLRKCL
110 120 130 140 150
ESGMKKEMIM SDAAVEQRRA LIKRKKREKI EAPPPGGQGL TEEQQALIQE
160 170 180 190 200
LMDAQMQTFD TTFSHFKDFR LPAVFHSGCE LPEFLQASLL EDPATWSQIM
210 220 230 240 250
KDRVPMKISL QLRGEDGSIW NYQPPSKSDG KEIIPLLPHL ADVSTYMFKG
260 270 280 290 300
VINFAKVISY FRDLPIEDQI SLLKGATFEM CILRFNTMFD TETGTWECGR
310 320 330 340 350
LAYCFEDPNG GFQKLLLDPL MKFHCMLKKL QLHKEEYVLM QAISLFSPDR
360 370 380 390 400
PGVVQRSVVD QLQERFALTL KAYIECSRPY PAHRFLFLKI MAVLTELRSI
410 420 430
NAQQTQQLLR IQDSHPFATP LMQELFSSTD G
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_003670 | 171 – 211 | Missing in isoform 2. CuratedAdd BLAST | 41 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF031814 mRNA Translation: AAC39964.1 AK018630 mRNA Translation: BAB31316.1 |
CCDSi | CCDS28164.1 [O54915-1] |
RefSeqi | NP_001091874.1, NM_001098404.1 [O54915-2] NP_035066.1, NM_010936.3 [O54915-1] XP_006521909.1, XM_006521846.3 [O54915-1] XP_006521911.1, XM_006521848.3 [O54915-1] |
Genome annotation databases
Ensembli | ENSMUST00000023504; ENSMUSP00000023504; ENSMUSG00000022809 [O54915-1] |
GeneIDi | 18171 |
KEGGi | mmu:18171 |
UCSCi | uc007zep.1, mouse [O54915-2] uc007zeq.1, mouse [O54915-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF031814 mRNA Translation: AAC39964.1 AK018630 mRNA Translation: BAB31316.1 |
CCDSi | CCDS28164.1 [O54915-1] |
RefSeqi | NP_001091874.1, NM_001098404.1 [O54915-2] NP_035066.1, NM_010936.3 [O54915-1] XP_006521909.1, XM_006521846.3 [O54915-1] XP_006521911.1, XM_006521848.3 [O54915-1] |
3D structure databases
SMRi | O54915 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201839, 5 interactors |
ComplexPortali | CPX-505, RXRalpha-PXR nuclear receptor complex CPX-644, PXR-NCOA1 activated nuclear receptor complex |
IntActi | O54915, 2 interactors |
STRINGi | 10090.ENSMUSP00000023504 |
Chemistry databases
ChEMBLi | CHEMBL1743244 |
DrugCentrali | O54915 |
GuidetoPHARMACOLOGYi | 606 |
PTM databases
PhosphoSitePlusi | O54915 |
Proteomic databases
PaxDbi | O54915 |
PRIDEi | O54915 |
ProteomicsDBi | 293717 [O54915-1] 293718 [O54915-2] |
Protocols and materials databases
Antibodypediai | 16619, 420 antibodies from 42 providers |
DNASUi | 18171 |
Genome annotation databases
Ensembli | ENSMUST00000023504; ENSMUSP00000023504; ENSMUSG00000022809 [O54915-1] |
GeneIDi | 18171 |
KEGGi | mmu:18171 |
UCSCi | uc007zep.1, mouse [O54915-2] uc007zeq.1, mouse [O54915-1] |
Organism-specific databases
CTDi | 8856 |
MGIi | MGI:1337040, Nr1i2 |
VEuPathDBi | HostDB:ENSMUSG00000022809 |
Phylogenomic databases
eggNOGi | KOG3575, Eukaryota |
GeneTreei | ENSGT00940000161118 |
HOGENOMi | CLU_007368_12_0_1 |
InParanoidi | O54915 |
OMAi | GSIWNYK |
PhylomeDBi | O54915 |
TreeFami | TF316304 |
Enzyme and pathway databases
Reactomei | R-MMU-383280, Nuclear Receptor transcription pathway R-MMU-4090294, SUMOylation of intracellular receptors |
Miscellaneous databases
BioGRID-ORCSi | 18171, 0 hits in 66 CRISPR screens |
ChiTaRSi | Nr1i2, mouse |
PROi | PR:O54915 |
RNActi | O54915, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022809, Expressed in liver and 77 other tissues |
ExpressionAtlasi | O54915, baseline and differential |
Genevisiblei | O54915, MM |
Family and domain databases
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
InterProi | View protein in InterPro IPR035500, NHR-like_dom_sf IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PRINTSi | PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NR1I2_MOUSE | |
Accessioni | O54915Primary (citable) accession number: O54915 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | June 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 169 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families