Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tumor necrosis factor ligand superfamily member 12

Gene

Tnfsf12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCytokine, Developmental protein
Biological processAngiogenesis, Apoptosis, Differentiation

Enzyme and pathway databases

ReactomeiR-MMU-5668541 TNFR2 non-canonical NF-kB pathway
R-MMU-5676594 TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Tumor necrosis factor ligand superfamily member 12
Alternative name(s):
TNF-related weak inducer of apoptosis
Short name:
TWEAK
Cleaved into the following 2 chains:
Gene namesi
Name:Tnfsf12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1196259 Tnfsf12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 21CytoplasmicSequence analysisAdd BLAST21
Transmembranei22 – 45Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST24
Topological domaini46 – 249ExtracellularSequence analysisAdd BLAST204

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000345221 – 249Tumor necrosis factor ligand superfamily member 12, membrane formAdd BLAST249
ChainiPRO_000003452394 – 249Tumor necrosis factor ligand superfamily member 12, secreted formBy similarityAdd BLAST156

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi139N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi191 ↔ 210By similarity

Post-translational modificationi

The soluble form is produced from the membrane form by proteolytic processing.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei93 – 94CleavageBy similarity2

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO54907
PRIDEiO54907

PTM databases

PhosphoSitePlusiO54907

Expressioni

Tissue specificityi

Widely expressed.

Gene expression databases

BgeeiENSMUSG00000097328 Expressed in 209 organ(s), highest expression level in retina
ExpressionAtlasiO54907 baseline and differential
GenevisibleiO54907 MM

Interactioni

Subunit structurei

Homotrimer. Interacts with the angiogenic factor AGGF1/VG5Q (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000137658

Structurei

3D structure databases

ProteinModelPortaliO54907
SMRiO54907
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tumor necrosis factor family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II60 Eukaryota
ENOG410Y4KC LUCA
GeneTreeiENSGT00530000063837
HOVERGENiHBG055510
InParanoidiO54907
KOiK05474
OMAiGPHPFHF
OrthoDBiEOG091G0MIB

Family and domain databases

CDDicd00184 TNF, 1 hit
Gene3Di2.60.120.40, 1 hit
InterProiView protein in InterPro
IPR006052 TNF_dom
IPR008983 Tumour_necrosis_fac-like_dom
PfamiView protein in Pfam
PF00229 TNF, 1 hit
SMARTiView protein in SMART
SM00207 TNF, 1 hit
SUPFAMiSSF49842 SSF49842, 1 hit
PROSITEiView protein in PROSITE
PS50049 TNF_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O54907-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAARRSQRRR GRRGEPGTAL LAPLVLSLGL ALACLGLLLV VVSLGSWATL
60 70 80 90 100
SAQEPSQEEL TAEDRREPPE LNPQTEESQD VVPFLEQLVR PRRSAPKGRK
110 120 130 140 150
ARPRRAIAAH YEVHPRPGQD GAQAGVDGTV SGWEETKINS SSPLRYDRQI
160 170 180 190 200
GEFTVIRAGL YYLYCQVHFD EGKAVYLKLD LLVNGVLALR CLEEFSATAA
210 220 230 240
SSPGPQLRLC QVSGLLPLRP GSSLRIRTLP WAHLKAAPFL TYFGLFQVH
Length:249
Mass (Da):27,441
Last modified:May 24, 2004 - v2
Checksum:i08352620444EFB7B
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
M9MMK6M9MMK6_MOUSE
Tumor necrosis factor ligand superf...
Tnfsf12
98Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030100 mRNA Translation: AAC53517.2
AK020909 mRNA Translation: BAB32249.1
CCDSiCCDS24907.1
RefSeqiNP_035744.1, NM_011614.3
UniGeneiMm.8983

Genome annotation databases

EnsembliENSMUST00000181810; ENSMUSP00000137972; ENSMUSG00000097328
GeneIDi21944
KEGGimmu:21944
UCSCiuc007jri.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030100 mRNA Translation: AAC53517.2
AK020909 mRNA Translation: BAB32249.1
CCDSiCCDS24907.1
RefSeqiNP_035744.1, NM_011614.3
UniGeneiMm.8983

3D structure databases

ProteinModelPortaliO54907
SMRiO54907
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000137658

PTM databases

PhosphoSitePlusiO54907

Proteomic databases

PaxDbiO54907
PRIDEiO54907

Protocols and materials databases

DNASUi21944
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000181810; ENSMUSP00000137972; ENSMUSG00000097328
GeneIDi21944
KEGGimmu:21944
UCSCiuc007jri.2 mouse

Organism-specific databases

CTDi8742
MGIiMGI:1196259 Tnfsf12

Phylogenomic databases

eggNOGiENOG410II60 Eukaryota
ENOG410Y4KC LUCA
GeneTreeiENSGT00530000063837
HOVERGENiHBG055510
InParanoidiO54907
KOiK05474
OMAiGPHPFHF
OrthoDBiEOG091G0MIB

Enzyme and pathway databases

ReactomeiR-MMU-5668541 TNFR2 non-canonical NF-kB pathway
R-MMU-5676594 TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway

Miscellaneous databases

ChiTaRSiTnfsf12 mouse
PROiPR:O54907
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000097328 Expressed in 209 organ(s), highest expression level in retina
ExpressionAtlasiO54907 baseline and differential
GenevisibleiO54907 MM

Family and domain databases

CDDicd00184 TNF, 1 hit
Gene3Di2.60.120.40, 1 hit
InterProiView protein in InterPro
IPR006052 TNF_dom
IPR008983 Tumour_necrosis_fac-like_dom
PfamiView protein in Pfam
PF00229 TNF, 1 hit
SMARTiView protein in SMART
SM00207 TNF, 1 hit
SUPFAMiSSF49842 SSF49842, 1 hit
PROSITEiView protein in PROSITE
PS50049 TNF_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTNF12_MOUSE
AccessioniPrimary (citable) accession number: O54907
Secondary accession number(s): Q9CTP2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: May 24, 2004
Last modified: November 7, 2018
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again