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Protein

Integrin beta-3

Gene

Itgb3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIB/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIB/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIB/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIB/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surfaces. Fibrinogen binding enhances SELP expression in activated platelets (PubMed:19332769). ITGAV:ITGB3 binds to fractalkine (CX3CL1) and acts as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling. ITGAV:ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (By similarity). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (By similarity). ITGAV:ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (By similarity). ITGAV:ITGB3 binds to IL1B and this binding is essential for IL1B signaling (By similarity). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (By similarity). ITGAV:ITGB3 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIntegrin, Receptor
Biological processCell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-114608 Platelet degranulation
R-MMU-1566948 Elastic fibre formation
R-MMU-2129379 Molecules associated with elastic fibres
R-MMU-216083 Integrin cell surface interactions
R-MMU-3000170 Syndecan interactions
R-MMU-3000178 ECM proteoglycans
R-MMU-354192 Integrin alphaIIb beta3 signaling
R-MMU-354194 GRB2:SOS provides linkage to MAPK signaling for Integrins
R-MMU-372708 p130Cas linkage to MAPK signaling for integrins
R-MMU-4420097 VEGFA-VEGFR2 Pathway
R-MMU-445144 Signal transduction by L1
R-MMU-5674135 MAP2K and MAPK activation

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin beta-3
Alternative name(s):
Platelet membrane glycoprotein IIIa
Short name:
GPIIIa
CD_antigen: CD61
Gene namesi
Name:Itgb3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:96612 Itgb3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 717ExtracellularSequence analysisAdd BLAST692
Transmembranei718 – 740HelicalSequence analysisAdd BLAST23
Topological domaini741 – 787CytoplasmicSequence analysisAdd BLAST47

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3430894

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000001634526 – 787Integrin beta-3Add BLAST762

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi30 ↔ 460By similarity
Disulfide bondi38 ↔ 48By similarity
Disulfide bondi41 ↔ 74By similarity
Disulfide bondi51 ↔ 63By similarity
Disulfide bondi202 ↔ 209By similarity
Disulfide bondi257 ↔ 298By similarity
Glycosylationi345N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi396N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi399 ↔ 411By similarity
Disulfide bondi431 ↔ 680By similarity
Disulfide bondi458 ↔ 462By similarity
Disulfide bondi473 ↔ 485By similarity
Glycosylationi477N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi482 ↔ 520By similarity
Disulfide bondi487 ↔ 496By similarity
Disulfide bondi498 ↔ 511By similarity
Disulfide bondi526 ↔ 531By similarity
Disulfide bondi528 ↔ 561By similarity
Disulfide bondi533 ↔ 546By similarity
Disulfide bondi548 ↔ 553By similarity
Disulfide bondi567 ↔ 572By similarity
Disulfide bondi569 ↔ 600By similarity
Disulfide bondi574 ↔ 583By similarity
Glycosylationi584N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi585 ↔ 592By similarity
Disulfide bondi606 ↔ 611By similarity
Disulfide bondi608 ↔ 656By similarity
Disulfide bondi613 ↔ 623By similarity
Disulfide bondi626 ↔ 629By similarity
Disulfide bondi633 ↔ 642By similarity
Disulfide bondi639 ↔ 712By similarity
Disulfide bondi660 ↔ 688By similarity
Glycosylationi679N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei766PhosphothreonineCombined sources1
Modified residuei772PhosphotyrosineBy similarity1
Modified residuei778PhosphothreonineBy similarity1
Modified residuei784PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiO54890
MaxQBiO54890
PaxDbiO54890
PeptideAtlasiO54890
PRIDEiO54890

PTM databases

iPTMnetiO54890
PhosphoSitePlusiO54890

Expressioni

Gene expression databases

BgeeiENSMUSG00000020689 Expressed in 132 organ(s), highest expression level in blood
CleanExiMM_ITGB3
GenevisibleiO54890 MM

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit (By similarity). Beta-3 (ITGB3) associates with either alpha-IIB (ITGA2B) or alpha-V (ITGAV). Interacts with FLNB and COMP (By similarity). Interacts with PDIA6 following platelet stimulation (By similarity). Interacts with SYK; upon activation by ITGB3 promotes platelet adhesion (By similarity). Interacts with MYO10 (By similarity). Interacts with DAB2 (PubMed:12606711). Interacts with FERMT2 (PubMed:18483218). Integrin ITGAV:ITGB3 interacts with FBLN5 (via N-terminus) (PubMed:11805835). Interacts with EMP2; regulates the levels of the heterodimer ITGA5:ITGB3 integrin expression on the plasma membrane (By similarity). ITGAV:ITGB3 interacts with NOV (By similarity). ITGAV:ITGB3 interacts with AGRA2 (By similarity). ITGAV:ITGB3 is found in a ternary complex with CX3CR1 and CX3CL1. ITGAV:ITGB3 is found in a ternary complex with NRG1 and ERBB3. ITGAV:ITGB3 is found in a ternary complex with FGF1 and FGFR1. ITGAV:ITGB3 interacts with FGF2; it is likely that FGF2 can simultaneously bind ITGAV:ITGB3 and FGF receptors (By similarity). ITGAV:ITGB3 binds to IL1B (By similarity). ITGAV:ITGB3 is found in a ternary complex with IGF1 and IGF1R (By similarity). ITGAV:ITGB3 interacts with IGF2 (By similarity). ITGAV:ITGB3 interacts with FBN1 (By similarity). ITGAV:ITGB3 interacts with CD9, CD81 and CD151 (via second extracellular domain) (By similarity). Interacts (via the allosteric site (site 2)) with CXCL12 in a CXCR4-independent manner (By similarity).By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200830, 5 interactors
ComplexPortaliCPX-3035 Integrin alphav-beta3 complex
CPX-3116 Integrin alphaIIb-beta3 complex
CORUMiO54890
DIPiDIP-46416N
IntActiO54890, 6 interactors
MINTiO54890
STRINGi10090.ENSMUSP00000021028

Structurei

Secondary structure

1787
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO54890
SMRiO54890
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini134 – 376VWFAAdd BLAST243
Repeati462 – 510IAdd BLAST49
Repeati511 – 552IIAdd BLAST42
Repeati553 – 591IIIAdd BLAST39
Repeati592 – 628IVAdd BLAST37

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni202 – 209CX3CL1-bindingBy similarity8
Regioni202 – 209Involved in CX3CL1-, NRG1-, FGF1- and IGF1-bindingBy similarity8
Regioni292 – 312CX3CL1-bindingBy similarityAdd BLAST21
Regioni462 – 628Cysteine-rich tandem repeatsAdd BLAST167

Sequence similaritiesi

Belongs to the integrin beta chain family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1226 Eukaryota
ENOG410XP60 LUCA
GeneTreeiENSGT00760000119064
HOGENOMiHOG000252936
HOVERGENiHBG006190
InParanoidiO54890
KOiK06493
OMAiPRCNLKE
OrthoDBiEOG091G029W
TreeFamiTF105392

Family and domain databases

Gene3Di3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR013111 EGF_extracell
IPR027068 Integrin_beta-3
IPR033760 Integrin_beta_N
IPR015812 Integrin_bsu
IPR014836 Integrin_bsu_cyt_dom
IPR012896 Integrin_bsu_tail
IPR036349 Integrin_bsu_tail_dom_sf
IPR002369 Integrin_bsu_VWA
IPR032695 Integrin_dom_sf
IPR016201 PSI
IPR036465 vWFA_dom_sf
PANTHERiPTHR10082 PTHR10082, 1 hit
PTHR10082:SF25 PTHR10082:SF25, 1 hit
PfamiView protein in Pfam
PF07974 EGF_2, 2 hits
PF08725 Integrin_b_cyt, 1 hit
PF07965 Integrin_B_tail, 1 hit
PF00362 Integrin_beta, 1 hit
PF17205 PSI_integrin, 1 hit
PIRSFiPIRSF002512 Integrin_B, 1 hit
PRINTSiPR01186 INTEGRINB
SMARTiView protein in SMART
SM00187 INB, 1 hit
SM01241 Integrin_b_cyt, 1 hit
SM01242 Integrin_B_tail, 1 hit
SM00423 PSI, 1 hit
SUPFAMiSSF53300 SSF53300, 1 hit
SSF69179 SSF69179, 1 hit
SSF69687 SSF69687, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 2 hits
PS01186 EGF_2, 1 hit
PS00243 INTEGRIN_BETA, 3 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O54890-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRAQWPGQLW AALLALGALA GVVVGESNIC TTRGVNSCQQ CLAVSPVCAW
60 70 80 90 100
CSDETLSQGS PRCNLKENLL KDNCAPESIE FPVSEAQILE ARPLSSKGSG
110 120 130 140 150
SSAQITQVSP QRIALRLRPD DSKIFSLQVR QVEDYPVDIY YLMDLSFSMK
160 170 180 190 200
DDLSSIQTLG TKLASQMRKL TSNLRIGFGA FVDKPVSPYM YISPPQAIKN
210 220 230 240 250
PCYNMKNACL PMFGYKHVLT LTDQVSRFNE EVKKQSVSRN RDAPEGGFDA
260 270 280 290 300
IMQATVCDEK IGWRNDASHL LVFTTDAKTH IALDGRLAGI VLPNDGHCHI
310 320 330 340 350
GTDNHYSAST TMDYPSLGLM TEKLSQKNIN LIFAVTENVV SLYQNYSELI
360 370 380 390 400
PGTTVGVLSD DSSNVLQLIV DAYGKIRSKV ELEVRDLPEE LSLSFNATCL
410 420 430 440 450
NNEVIPGLKS CVGLKIGDTV SFSIEAKVRG CPQEKEQSFT IKPVGFKDSL
460 470 480 490 500
TVQVTFDCDC ACQAFAQPSS PRCNNGNGTF ECGVCRCDQG WLGSMCECSE
510 520 530 540 550
EDYRPSQQEE CSPKEGQPIC SQRGECLCGQ CVCHSSDFGK ITGKYCECDD
560 570 580 590 600
FSCVRYKGEM CSGHGQCNCG DCVCDSDWTG YYCNCTTRTD TCMSTNGLLC
610 620 630 640 650
SGRGNCECGS CVCVQPGSYG DTCEKCPTCP DACSFKKECV ECKKFNRGTL
660 670 680 690 700
HEENTCSRYC RDDIEQVKEL TDTGKNAVNC TYKNEDDCVV RFQYYEDTSG
710 720 730 740 750
RAVLYVVEEP ECPKGPDILV VLLSVMGAIL LIGLATLLIW KLLITIHDRK
760 770 780
EFAKFEEERA RAKWDTANNP LYKEATSTFT NITYRGT
Length:787
Mass (Da):86,738
Last modified:July 27, 2011 - v2
Checksum:i4F2E606969788794
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti114A → V in AAB94086 (Ref. 1) Curated1
Sequence conflicti389E → G in AAB94086 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF026509 mRNA Translation: AAB94086.1
AK157958 mRNA Translation: BAE34283.1
AL603709, BX000996 Genomic DNA Translation: CAM22629.1
BX000996, AL603709 Genomic DNA Translation: CAM27926.1
CH466558 Genomic DNA Translation: EDL34227.1
BC125518 mRNA Translation: AAI25519.1
BC125520 mRNA Translation: AAI25521.1
CCDSiCCDS25536.1
PIRiPN0510
RefSeqiNP_058060.2, NM_016780.2
UniGeneiMm.87150

Genome annotation databases

EnsembliENSMUST00000021028; ENSMUSP00000021028; ENSMUSG00000020689
GeneIDi16416
KEGGimmu:16416
UCSCiuc007lwx.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF026509 mRNA Translation: AAB94086.1
AK157958 mRNA Translation: BAE34283.1
AL603709, BX000996 Genomic DNA Translation: CAM22629.1
BX000996, AL603709 Genomic DNA Translation: CAM27926.1
CH466558 Genomic DNA Translation: EDL34227.1
BC125518 mRNA Translation: AAI25519.1
BC125520 mRNA Translation: AAI25521.1
CCDSiCCDS25536.1
PIRiPN0510
RefSeqiNP_058060.2, NM_016780.2
UniGeneiMm.87150

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5XQ1X-ray2.95A/B773-787[»]
ProteinModelPortaliO54890
SMRiO54890
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200830, 5 interactors
ComplexPortaliCPX-3035 Integrin alphav-beta3 complex
CPX-3116 Integrin alphaIIb-beta3 complex
CORUMiO54890
DIPiDIP-46416N
IntActiO54890, 6 interactors
MINTiO54890
STRINGi10090.ENSMUSP00000021028

Chemistry databases

ChEMBLiCHEMBL3430894

PTM databases

iPTMnetiO54890
PhosphoSitePlusiO54890

Proteomic databases

EPDiO54890
MaxQBiO54890
PaxDbiO54890
PeptideAtlasiO54890
PRIDEiO54890

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021028; ENSMUSP00000021028; ENSMUSG00000020689
GeneIDi16416
KEGGimmu:16416
UCSCiuc007lwx.2 mouse

Organism-specific databases

CTDi3690
MGIiMGI:96612 Itgb3

Phylogenomic databases

eggNOGiKOG1226 Eukaryota
ENOG410XP60 LUCA
GeneTreeiENSGT00760000119064
HOGENOMiHOG000252936
HOVERGENiHBG006190
InParanoidiO54890
KOiK06493
OMAiPRCNLKE
OrthoDBiEOG091G029W
TreeFamiTF105392

Enzyme and pathway databases

ReactomeiR-MMU-114608 Platelet degranulation
R-MMU-1566948 Elastic fibre formation
R-MMU-2129379 Molecules associated with elastic fibres
R-MMU-216083 Integrin cell surface interactions
R-MMU-3000170 Syndecan interactions
R-MMU-3000178 ECM proteoglycans
R-MMU-354192 Integrin alphaIIb beta3 signaling
R-MMU-354194 GRB2:SOS provides linkage to MAPK signaling for Integrins
R-MMU-372708 p130Cas linkage to MAPK signaling for integrins
R-MMU-4420097 VEGFA-VEGFR2 Pathway
R-MMU-445144 Signal transduction by L1
R-MMU-5674135 MAP2K and MAPK activation

Miscellaneous databases

ChiTaRSiItgb3 mouse
PROiPR:O54890
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020689 Expressed in 132 organ(s), highest expression level in blood
CleanExiMM_ITGB3
GenevisibleiO54890 MM

Family and domain databases

Gene3Di3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR013111 EGF_extracell
IPR027068 Integrin_beta-3
IPR033760 Integrin_beta_N
IPR015812 Integrin_bsu
IPR014836 Integrin_bsu_cyt_dom
IPR012896 Integrin_bsu_tail
IPR036349 Integrin_bsu_tail_dom_sf
IPR002369 Integrin_bsu_VWA
IPR032695 Integrin_dom_sf
IPR016201 PSI
IPR036465 vWFA_dom_sf
PANTHERiPTHR10082 PTHR10082, 1 hit
PTHR10082:SF25 PTHR10082:SF25, 1 hit
PfamiView protein in Pfam
PF07974 EGF_2, 2 hits
PF08725 Integrin_b_cyt, 1 hit
PF07965 Integrin_B_tail, 1 hit
PF00362 Integrin_beta, 1 hit
PF17205 PSI_integrin, 1 hit
PIRSFiPIRSF002512 Integrin_B, 1 hit
PRINTSiPR01186 INTEGRINB
SMARTiView protein in SMART
SM00187 INB, 1 hit
SM01241 Integrin_b_cyt, 1 hit
SM01242 Integrin_B_tail, 1 hit
SM00423 PSI, 1 hit
SUPFAMiSSF53300 SSF53300, 1 hit
SSF69179 SSF69179, 1 hit
SSF69687 SSF69687, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 2 hits
PS01186 EGF_2, 1 hit
PS00243 INTEGRIN_BETA, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiITB3_MOUSE
AccessioniPrimary (citable) accession number: O54890
Secondary accession number(s): Q3TZC6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 167 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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