UniProtKB - O54885 (TYOBP_MOUSE)
TYRO protein tyrosine kinase-binding protein
Tyrobp
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 52 | Calcium; shared with neighboring subunit in homooligomerBy similarity | 1 | |
Sitei | 56 | Important for interaction with transmembrane receptorsBy similarity | 1 |
GO - Molecular functioni
- identical protein binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- protein homodimerization activity Source: UniProtKB
- signaling receptor binding Source: ARUK-UCL
GO - Biological processi
- actin cytoskeleton organization Source: UniProtKB
- apoptotic cell clearance Source: UniProtKB
- apoptotic signaling pathway Source: ARUK-UCL
- forebrain development Source: ARUK-UCL
- integrin-mediated signaling pathway Source: UniProtKB
- macrophage activation involved in immune response Source: UniProtKB
- microglial cell activation involved in immune response Source: UniProtKB
- myeloid leukocyte activation Source: MGI
- negative regulation of B cell proliferation Source: UniProtKB
- negative regulation of interleukin-10 production Source: ARUK-UCL
- negative regulation of long-term synaptic potentiation Source: ARUK-UCL
- negative regulation of transforming growth factor beta1 production Source: ARUK-UCL
- neutrophil activation involved in immune response Source: UniProtKB
- osteoclast differentiation Source: MGI
- positive regulation of gene expression Source: ARUK-UCL
- positive regulation of hippocampal neuron apoptotic process Source: ARUK-UCL
- positive regulation of interleukin-1 beta production Source: ARUK-UCL
- positive regulation of interleukin-6 production Source: ARUK-UCL
- positive regulation of macrophage fusion Source: UniProtKB
- positive regulation of microglial cell mediated cytotoxicity Source: UniProtKB
- positive regulation of natural killer cell activation Source: UniProtKB
- positive regulation of neuron death Source: ARUK-UCL
- positive regulation of osteoclast development Source: UniProtKB
- positive regulation of protein localization to cell surface Source: MGI
- positive regulation of receptor localization to synapse Source: ARUK-UCL
- positive regulation of superoxide anion generation Source: ARUK-UCL
- positive regulation of tumor necrosis factor production Source: ARUK-UCL
- protein stabilization Source: UniProtKB
- regulation of osteoclast development Source: MGI
- response to axon injury Source: ARUK-UCL
- stimulatory C-type lectin receptor signaling pathway Source: UniProtKB
- stimulatory killer cell immunoglobulin-like receptor signaling pathway Source: UniProtKB
Keywordsi
Biological process | Immunity |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-2172127, DAP12 interactions R-MMU-2424491, DAP12 signaling R-MMU-391160, Signal regulatory protein family interactions R-MMU-416700, Other semaphorin interactions R-MMU-6798695, Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | Recommended name: TYRO protein tyrosine kinase-binding proteinImportedAlternative name(s): DNAX-activation protein 121 Publication Killer-activating receptor-associated protein1 Publication Short name: KAR-associated protein1 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1277211, Tyrobp |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Single-pass type I membrane protein Sequence analysis
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
- plasma membrane Source: MGI
Other locations
- cell surface Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 22 – 42 | ExtracellularBy similarityAdd BLAST | 21 | |
Transmembranei | 43 – 63 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 64 – 114 | CytoplasmicBy similarityAdd BLAST | 51 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 52 | D → A: Failure to rescue cytoskeletal defects when introduced into TYROBP-deficient cells. 1 Publication | 1 | |
Mutagenesisi | 92 | Y → F: Abolishes phosphorylation and ability to regulate cytoskeletal organization; when associated with F-103. 2 Publications | 1 | |
Mutagenesisi | 103 | Y → F: Abolishes phosphorylation and ability to regulate cytoskeletal organization; when associated with F-92. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
ChainiPRO_0000022605 | 22 – 114 | TYRO protein tyrosine kinase-binding proteinAdd BLAST | 93 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 37 | InterchainBy similarity | ||
Modified residuei | 92 | PhosphotyrosineCombined sources2 Publications | 1 | |
Modified residuei | 103 | PhosphotyrosineCombined sources2 Publications | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, PhosphoproteinProteomic databases
PaxDbi | O54885 |
PeptideAtlasi | O54885 |
PRIDEi | O54885 |
ProteomicsDBi | 298443 |
PTM databases
iPTMneti | O54885 |
PhosphoSitePlusi | O54885 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000030579, Expressed in granulocyte and 252 other tissues |
Genevisiblei | O54885, MM |
Interactioni
Subunit structurei
Homodimer; disulfide-linked (By similarity). Homotrimer; disulfide-linked (By similarity). Homotetramer; disulfide-linked (By similarity). Homotrimers and homotetramers form when low levels of partner receptors are available and are competitive with assembly with interacting receptors (By similarity). They may represent alternative oligomerization states or may be intermediates in the receptor assembly process (By similarity). Binding of a metal cation aids in homooligomerization through coordination of the metal ion by the subunits of the oligomer (By similarity).
Interacts with TREM1 (By similarity).
Interacts with TREM2 (PubMed:29518356).
Interacts with TREM3 (PubMed:11754004).
Interacts with CLECSF5 (PubMed:10449773).
Interacts with CD300LB and CD300C2 (PubMed:12874256, PubMed:12893283, PubMed:17202337, PubMed:17928527).
Interacts with CD300E (By similarity).
Interacts (via ITAM domain) with SYK (via SH2 domains); activates SYK mediating neutrophil and macrophage integrin-mediated activation (PubMed:17086186).
Interacts (via transmembrane domain) with KLRK1 isoform 2 (via transmembrane domain); the interaction is required for KLRK1 NK cell surface expression and NK cell-mediated cytotoxicity (PubMed:12426564, PubMed:12426565, PubMed:15294961).
Interacts with KLRC2 (By similarity).
Interacts with CD300H (By similarity).
Interacts with KLRD1 (By similarity).
Interacts with KLRA4 and KLRA8 (PubMed:9647200).
By similarity12 PublicationsBinary interactionsi
Hide detailsO54885
With | #Exp. | IntAct |
---|---|---|
Clec5a [Q9R007] | 3 | EBI-15687058,EBI-15761206 |
Hcst [Q9QUJ0] | 4 | EBI-15687058,EBI-15761243 |
GO - Molecular functioni
- identical protein binding Source: MGI
- protein homodimerization activity Source: UniProtKB
- signaling receptor binding Source: ARUK-UCL
Protein-protein interaction databases
BioGRIDi | 204396, 6 interactors |
CORUMi | O54885 |
DIPi | DIP-29746N |
IntActi | O54885, 4 interactors |
STRINGi | 10090.ENSMUSP00000032800 |
Miscellaneous databases
RNActi | O54885, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 81 – 109 | ITAMAdd BLAST | 29 |
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502SCVI, Eukaryota |
GeneTreei | ENSGT00390000016786 |
HOGENOMi | CLU_141718_0_0_1 |
InParanoidi | O54885 |
OrthoDBi | 1508977at2759 |
PhylomeDBi | O54885 |
TreeFami | TF336898 |
Family and domain databases
InterProi | View protein in InterPro IPR026200, Tyrobp |
PANTHERi | PTHR17554, PTHR17554, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MGALEPSWCL LFLPVLLTVG GLSPVQAQSD TFPRCDCSSV SPGVLAGIVL
60 70 80 90 100
GDLVLTLLIA LAVYSLGRLV SRGQGTAEGT RKQHIAETES PYQELQGQRP
110
EVYSDLNTQR QYYR
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A140LI31 | A0A140LI31_MOUSE | DNAX-activation protein 12 | Tyrobp | 92 | Annotation score: | ||
A0A140LHP7 | A0A140LHP7_MOUSE | DNAX-activation protein 12 | Tyrobp | 141 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 20 | G → E in AAC95529 (PubMed:9852069).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF024637 mRNA Translation: AAD09438.1 AF077829 mRNA Translation: AAC95529.1 BC056450 mRNA Translation: AAH56450.1 |
CCDSi | CCDS21089.1 |
RefSeqi | NP_035792.1, NM_011662.2 |
Genome annotation databases
Ensembli | ENSMUST00000032800; ENSMUSP00000032800; ENSMUSG00000030579 |
GeneIDi | 22177 |
KEGGi | mmu:22177 |
UCSCi | uc009gef.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF024637 mRNA Translation: AAD09438.1 AF077829 mRNA Translation: AAC95529.1 BC056450 mRNA Translation: AAH56450.1 |
CCDSi | CCDS21089.1 |
RefSeqi | NP_035792.1, NM_011662.2 |
3D structure databases
SMRi | O54885 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204396, 6 interactors |
CORUMi | O54885 |
DIPi | DIP-29746N |
IntActi | O54885, 4 interactors |
STRINGi | 10090.ENSMUSP00000032800 |
PTM databases
iPTMneti | O54885 |
PhosphoSitePlusi | O54885 |
Proteomic databases
PaxDbi | O54885 |
PeptideAtlasi | O54885 |
PRIDEi | O54885 |
ProteomicsDBi | 298443 |
Protocols and materials databases
Antibodypediai | 4010, 295 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000032800; ENSMUSP00000032800; ENSMUSG00000030579 |
GeneIDi | 22177 |
KEGGi | mmu:22177 |
UCSCi | uc009gef.1, mouse |
Organism-specific databases
CTDi | 7305 |
MGIi | MGI:1277211, Tyrobp |
Phylogenomic databases
eggNOGi | ENOG502SCVI, Eukaryota |
GeneTreei | ENSGT00390000016786 |
HOGENOMi | CLU_141718_0_0_1 |
InParanoidi | O54885 |
OrthoDBi | 1508977at2759 |
PhylomeDBi | O54885 |
TreeFami | TF336898 |
Enzyme and pathway databases
Reactomei | R-MMU-2172127, DAP12 interactions R-MMU-2424491, DAP12 signaling R-MMU-391160, Signal regulatory protein family interactions R-MMU-416700, Other semaphorin interactions R-MMU-6798695, Neutrophil degranulation |
Miscellaneous databases
BioGRID-ORCSi | 22177, 1 hit in 52 CRISPR screens |
ChiTaRSi | Tyrobp, mouse |
PROi | PR:O54885 |
RNActi | O54885, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030579, Expressed in granulocyte and 252 other tissues |
Genevisiblei | O54885, MM |
Family and domain databases
InterProi | View protein in InterPro IPR026200, Tyrobp |
PANTHERi | PTHR17554, PTHR17554, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TYOBP_MOUSE | |
Accessioni | O54885Primary (citable) accession number: O54885 Secondary accession number(s): O88603 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | June 1, 1998 | |
Last modified: | April 7, 2021 | |
This is version 151 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families