UniProtKB - O54885 (TYOBP_MOUSE)
Protein
TYRO protein tyrosine kinase-binding protein
Gene
Tyrobp
Organism
Mus musculus (Mouse)
Status
Functioni
Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation. Involved for instance in neutrophil activation mediated by integrin.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 56 | Important for interaction with transmembrane receptorsBy similarity | 1 |
GO - Molecular functioni
- identical protein binding Source: MGI
- signaling receptor binding Source: ARUK-UCL
GO - Biological processi
- actin cytoskeleton organization Source: UniProtKB
- apoptotic signaling pathway Source: ARUK-UCL
- forebrain development Source: ARUK-UCL
- integrin-mediated signaling pathway Source: UniProtKB
- macrophage activation involved in immune response Source: UniProtKB
- microglial cell activation involved in immune response Source: UniProtKB
- myeloid leukocyte activation Source: MGI
- negative regulation of B cell proliferation Source: UniProtKB
- negative regulation of long-term synaptic potentiation Source: ARUK-UCL
- neutrophil activation involved in immune response Source: UniProtKB
- osteoclast differentiation Source: MGI
- positive regulation of hippocampal neuron apoptotic process Source: ARUK-UCL
- positive regulation of macrophage fusion Source: UniProtKB
- positive regulation of microglial cell mediated cytotoxicity Source: UniProtKB
- positive regulation of natural killer cell activation Source: UniProtKB
- positive regulation of osteoclast development Source: UniProtKB
- positive regulation of protein localization to cell surface Source: MGI
- positive regulation of receptor localization to synapse Source: ARUK-UCL
- positive regulation of superoxide anion generation Source: ARUK-UCL
- protein stabilization Source: UniProtKB
- regulation of osteoclast development Source: MGI
Enzyme and pathway databases
Reactomei | R-MMU-2172127 DAP12 interactions R-MMU-2424491 DAP12 signaling R-MMU-391160 Signal regulatory protein family interactions R-MMU-416700 Other semaphorin interactions R-MMU-6798695 Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | Recommended name: TYRO protein tyrosine kinase-binding proteinAlternative name(s): DNAX-activation protein 12 Killer-activating receptor-associated protein Short name: KAR-associated protein |
Gene namesi | Name:Tyrobp Synonyms:Dap12, Karap |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1277211 Tyrobp |
Subcellular locationi
Other locations
Plasma Membrane
- plasma membrane Source: Reactome
Other locations
- cell Source: ARUK-UCL
- cell surface Source: UniProtKB
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 22 – 42 | ExtracellularSequence analysisAdd BLAST | 21 | |
Transmembranei | 43 – 63 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 64 – 114 | CytoplasmicSequence analysisAdd BLAST | 51 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
ChainiPRO_0000022605 | 22 – 114 | TYRO protein tyrosine kinase-binding proteinAdd BLAST | 93 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 37 | InterchainBy similarity | ||
Modified residuei | 92 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 103 | PhosphotyrosineCombined sources | 1 |
Post-translational modificationi
Tyrosine phosphorylated.5 Publications
Keywords - PTMi
Disulfide bond, PhosphoproteinProteomic databases
PaxDbi | O54885 |
PeptideAtlasi | O54885 |
PRIDEi | O54885 |
PTM databases
iPTMneti | O54885 |
PhosphoSitePlusi | O54885 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000030579 Expressed in 231 organ(s), highest expression level in bone marrow |
ExpressionAtlasi | O54885 baseline and differential |
Genevisiblei | O54885 MM |
Interactioni
Subunit structurei
Homodimer; disulfide-linked. Interacts with SIRPB1 and TREM1. Interacts with SIGLEC14 (By similarity). Interacts with CLECSF5 (PubMed:10449773). Interacts with CD300LB and CD300D (PubMed:12874256, PubMed:12893283, PubMed:17202337, PubMed:17928527). Interacts with CD300E. Interacts (via ITAM domain) with SYK (via SH2 domains); activates SYK mediating neutrophils and macrophages integrin-mediated activation (PubMed:17086186). Interacts (via transmembrane domain) with KLRK1 isoform 2 (via transmembrane domain); the interaction is required for KLRK1 NK cell surface expression and induce NK cell-mediated cytotoxicity (PubMed:12426564, PubMed:12426565, PubMed:15294961). Interacts with KLRC2 (By similarity). Interacts with TREM3 (PubMed:11754004). Interacts with CD300H (By similarity).By similarity10 Publications
Binary interactionsi
GO - Molecular functioni
- identical protein binding Source: MGI
- signaling receptor binding Source: ARUK-UCL
Protein-protein interaction databases
BioGridi | 204396, 5 interactors |
CORUMi | O54885 |
DIPi | DIP-29746N |
IntActi | O54885, 4 interactors |
STRINGi | 10090.ENSMUSP00000032800 |
Structurei
3D structure databases
ProteinModelPortali | O54885 |
SMRi | O54885 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 81 – 109 | ITAMAdd BLAST | 29 |
Sequence similaritiesi
Belongs to the TYROBP family.Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG410J1XS Eukaryota ENOG410Z4TN LUCA |
GeneTreei | ENSGT00390000016786 |
HOGENOMi | HOG000056440 |
HOVERGENi | HBG061468 |
InParanoidi | O54885 |
KOi | K07992 |
OrthoDBi | 1508977at2759 |
PhylomeDBi | O54885 |
TreeFami | TF336898 |
Family and domain databases
InterProi | View protein in InterPro IPR026200 Tyrobp |
PANTHERi | PTHR17554 PTHR17554, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
O54885-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGALEPSWCL LFLPVLLTVG GLSPVQAQSD TFPRCDCSSV SPGVLAGIVL
60 70 80 90 100
GDLVLTLLIA LAVYSLGRLV SRGQGTAEGT RKQHIAETES PYQELQGQRP
110
EVYSDLNTQR QYYR
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A140LI31 | A0A140LI31_MOUSE | TYRO protein tyrosine kinase-bindin... | Tyrobp | 92 | Annotation score: | ||
A0A140LHP7 | A0A140LHP7_MOUSE | TYRO protein tyrosine kinase-bindin... | Tyrobp | 141 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 20 | G → E in AAC95529 (PubMed:9852069).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF024637 mRNA Translation: AAD09438.1 AF077829 mRNA Translation: AAC95529.1 BC056450 mRNA Translation: AAH56450.1 |
CCDSi | CCDS21089.1 |
RefSeqi | NP_035792.1, NM_011662.2 |
UniGenei | Mm.46301 |
Genome annotation databases
Ensembli | ENSMUST00000032800; ENSMUSP00000032800; ENSMUSG00000030579 |
GeneIDi | 22177 |
KEGGi | mmu:22177 |
UCSCi | uc009gef.1 mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF024637 mRNA Translation: AAD09438.1 AF077829 mRNA Translation: AAC95529.1 BC056450 mRNA Translation: AAH56450.1 |
CCDSi | CCDS21089.1 |
RefSeqi | NP_035792.1, NM_011662.2 |
UniGenei | Mm.46301 |
3D structure databases
ProteinModelPortali | O54885 |
SMRi | O54885 |
ModBasei | Search... |
MobiDBi | Search... |
Protein-protein interaction databases
BioGridi | 204396, 5 interactors |
CORUMi | O54885 |
DIPi | DIP-29746N |
IntActi | O54885, 4 interactors |
STRINGi | 10090.ENSMUSP00000032800 |
PTM databases
iPTMneti | O54885 |
PhosphoSitePlusi | O54885 |
Proteomic databases
PaxDbi | O54885 |
PeptideAtlasi | O54885 |
PRIDEi | O54885 |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Genome annotation databases
Ensembli | ENSMUST00000032800; ENSMUSP00000032800; ENSMUSG00000030579 |
GeneIDi | 22177 |
KEGGi | mmu:22177 |
UCSCi | uc009gef.1 mouse |
Organism-specific databases
CTDi | 7305 |
MGIi | MGI:1277211 Tyrobp |
Phylogenomic databases
eggNOGi | ENOG410J1XS Eukaryota ENOG410Z4TN LUCA |
GeneTreei | ENSGT00390000016786 |
HOGENOMi | HOG000056440 |
HOVERGENi | HBG061468 |
InParanoidi | O54885 |
KOi | K07992 |
OrthoDBi | 1508977at2759 |
PhylomeDBi | O54885 |
TreeFami | TF336898 |
Enzyme and pathway databases
Reactomei | R-MMU-2172127 DAP12 interactions R-MMU-2424491 DAP12 signaling R-MMU-391160 Signal regulatory protein family interactions R-MMU-416700 Other semaphorin interactions R-MMU-6798695 Neutrophil degranulation |
Miscellaneous databases
PROi | PR:O54885 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030579 Expressed in 231 organ(s), highest expression level in bone marrow |
ExpressionAtlasi | O54885 baseline and differential |
Genevisiblei | O54885 MM |
Family and domain databases
InterProi | View protein in InterPro IPR026200 Tyrobp |
PANTHERi | PTHR17554 PTHR17554, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | TYOBP_MOUSE | |
Accessioni | O54885Primary (citable) accession number: O54885 Secondary accession number(s): O88603 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | June 1, 1998 | |
Last modified: | February 13, 2019 | |
This is version 137 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot