Tyrobp

Functionⁱ

Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation. Involved for instance in neutrophil activation mediated by integrin.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	56	Important for interaction with transmembrane receptorsBy similarity		1

GO - Molecular functionⁱ

identical protein binding Source: MGI
signaling receptor binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

integrin-mediated signaling pathway
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 17086186
macrophage activation involved in immune response
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 17086186
neutrophil activation involved in immune response
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 17086186
osteoclast differentiation
Source: MGI
Inferred from direct assayⁱ
- 21841309
regulation of osteoclast development
Source: MGI
Inferred from genetic interactionⁱ
- 22451653

View the complete GO annotation on QuickGO ...

Enzyme and pathway databases

Reactomeⁱ	R-MMU-2172127 DAP12 interactions R-MMU-2424491 DAP12 signaling R-MMU-391160 Signal regulatory protein family interactions R-MMU-416700 Other semaphorin interactions R-MMU-6798695 Neutrophil degranulation

Reactomeⁱ

R-MMU-2172127 DAP12 interactions
R-MMU-2424491 DAP12 signaling
R-MMU-391160 Signal regulatory protein family interactions
R-MMU-416700 Other semaphorin interactions
R-MMU-6798695 Neutrophil degranulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: TYRO protein tyrosine kinase-binding protein Alternative name(s): DNAX-activation protein 12 Killer-activating receptor-associated protein Short name: KAR-associated protein
Gene namesⁱ	Name:Tyrobp Synonyms:Dap12, Karap
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 7

Organism-specific databases

MGIⁱ	MGI:1277211 Tyrobp

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	22 – 42	ExtracellularSequence analysisAdd BLAST	21
Transmembraneⁱ	43 – 63	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	64 – 114	CytoplasmicSequence analysisAdd BLAST	51

Keywords - Cellular componentⁱ

Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 21	Sequence analysisAdd BLAST		21
ChainⁱPRO_0000022605	22 – 114	TYRO protein tyrosine kinase-binding proteinAdd BLAST		93

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	37	InterchainBy similarity
Modified residueⁱ	92	PhosphotyrosineCombined sources	1
Modified residueⁱ	103	PhosphotyrosineCombined sources	1

Post-translational modificationⁱ

Tyrosine phosphorylated.5 Publications

Keywords - PTMⁱ

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbⁱ	O54885
PeptideAtlas More...PeptideAtlasⁱ	O54885
PRIDEⁱ	O54885

PTM databases

iPTMnetⁱ	O54885
PhosphoSitePlusⁱ	O54885

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000030579 Expressed in 231 organ(s), highest expression level in bone marrow
CleanExⁱ	MM_TYROBP
ExpressionAtlasⁱ	O54885 baseline and differential
Genevisibleⁱ	O54885 MM

Interactionⁱ

Subunit structureⁱ

Homodimer; disulfide-linked. Interacts with SIRPB1 and TREM1. Interacts with SIGLEC14 (By similarity). Interacts with CLECSF5 (PubMed:10449773). Interacts with CD300LB and CD300D (PubMed:12874256, PubMed:12893283, PubMed:17202337, PubMed:17928527). Interacts with CD300E. Interacts (via ITAM domain) with SYK (via SH2 domains); activates SYK mediating neutrophils and macrophages integrin-mediated activation (PubMed:17086186). Interacts (via transmembrane domain) with KLRK1 isoform 2 (via transmembrane domain); the interaction is required for KLRK1 NK cell surface expression and induce NK cell-mediated cytotoxicity (PubMed:12426564, PubMed:12426565, PubMed:15294961). Interacts with KLRC2 (By similarity). Interacts with TREM3 (PubMed:11754004). Interacts with CD300H (By similarity).By similarity10 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Clec5a	Q9R007	3	EBI-15687058,EBI-15761206
Hcst	Q9QUJ0	4	EBI-15687058,EBI-15761243

GO - Molecular functionⁱ

identical protein binding Source: MGI
signaling receptor binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	204396, 5 interactors
Database of interacting proteins More...DIPⁱ	DIP-29746N
IntActⁱ	O54885, 4 interactors
STRINGⁱ	10090.ENSMUSP00000032800

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O54885
SMRⁱ	O54885
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	81 – 109	ITAMAdd BLAST		29

Sequence similaritiesⁱ

Belongs to the TYROBP family.Curated

Keywords - Domainⁱ

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG410J1XS Eukaryota ENOG410Z4TN LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000016786
HOGENOMⁱ	HOG000056440
HOVERGENⁱ	HBG061468
InParanoidⁱ	O54885
KEGG Orthology (KO) More...KOⁱ	K07992
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0X44
PhylomeDBⁱ	O54885
TreeFamⁱ	TF336898

Family and domain databases

InterProⁱ	View protein in InterPro IPR026200 Tyrobp
PANTHERⁱ	PTHR17554 PTHR17554, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show all Align All

O54885-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MGALEPSWCL LFLPVLLTVG GLSPVQAQSD TFPRCDCSSV SPGVLAGIVL 
        60         70         80         90        100
GDLVLTLLIA LAVYSLGRLV SRGQGTAEGT RKQHIAETES PYQELQGQRP 
       110 
EVYSDLNTQR QYYR

Length:114

Mass (Da):12,367

Last modified:June 1, 1998 - v1

Checksum:ⁱ6A530D52E51FED8F

GO

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

A0A140LI31	A0A140LI31_MOUSE	TYRO protein tyrosine kinase-bindin... TYRO protein tyrosine kinase-binding protein	Tyrobp	92	Annotation score:
A0A140LHP7	A0A140LHP7_MOUSE	TYRO protein tyrosine kinase-bindin... TYRO protein tyrosine kinase-binding protein	Tyrobp	141	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	20	G → E in AAC95529 (PubMed:9852069).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF024637 mRNA Translation: AAD09438.1 AF077829 mRNA Translation: AAC95529.1 BC056450 mRNA Translation: AAH56450.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS21089.1
NCBI Reference Sequences More...RefSeqⁱ	NP_035792.1, NM_011662.2
UniGeneⁱ	Mm.46301

Genome annotation databases

Ensemblⁱ	ENSMUST00000032800; ENSMUSP00000032800; ENSMUSG00000030579
GeneIDⁱ	22177
KEGGⁱ	mmu:22177
UCSC genome browser More...UCSCⁱ	uc009gef.1 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O54885	TYRO protein tyrosine kinase-binding protein (Fragment)		MOUSE		141	UniRef100_O54885
TYRO protein tyrosine kinase binding protein, isoform CRA_b		MOUSE		114

Protein	Similar proteins		Species	Score	Length	Source
O54885	TYRO protein tyrosine kinase-binding protein		RAT		114	UniRef90_O54885
TYRO protein tyrosine kinase-binding protein (Fragment)		MOUSE		141
UPI000A30EFA3		MUSPA		114
TYRO protein tyrosine kinase binding protein, isoform CRA_b		MOUSE		114

Protein	Similar proteins		Species	Score	Length	Source
O54885	TYRO protein tyrosine kinase-binding protein		RAT		114	UniRef50_O54885
TYRO protein tyrosine kinase-binding protein (Fragment)		MOUSE		141
TYRO protein tyrosine kinase-binding protein isoform X2		Enhydra lutris kenyoni		101
TYRO protein tyrosine kinase binding protein		CANLF		113
TYRO protein tyrosine kinase-binding protein isoform X3		ODORO		102
+52

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF024637 mRNA Translation: AAD09438.1 AF077829 mRNA Translation: AAC95529.1 BC056450 mRNA Translation: AAH56450.1
CCDSⁱ	CCDS21089.1
RefSeqⁱ	NP_035792.1, NM_011662.2
UniGeneⁱ	Mm.46301

3D structure databases

ProteinModelPortalⁱ	O54885
SMRⁱ	O54885
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	204396, 5 interactors
DIPⁱ	DIP-29746N
IntActⁱ	O54885, 4 interactors
STRINGⁱ	10090.ENSMUSP00000032800

PTM databases

iPTMnetⁱ	O54885
PhosphoSitePlusⁱ	O54885

Proteomic databases

PaxDbⁱ	O54885
PeptideAtlasⁱ	O54885
PRIDEⁱ	O54885

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000032800; ENSMUSP00000032800; ENSMUSG00000030579
GeneIDⁱ	22177
KEGGⁱ	mmu:22177
UCSCⁱ	uc009gef.1 mouse

Organism-specific databases

CTDⁱ	7305
MGIⁱ	MGI:1277211 Tyrobp

Phylogenomic databases

eggNOGⁱ	ENOG410J1XS Eukaryota ENOG410Z4TN LUCA
GeneTreeⁱ	ENSGT00390000016786
HOGENOMⁱ	HOG000056440
HOVERGENⁱ	HBG061468
InParanoidⁱ	O54885
KOⁱ	K07992
OrthoDBⁱ	EOG091G0X44
PhylomeDBⁱ	O54885
TreeFamⁱ	TF336898

Enzyme and pathway databases

Reactomeⁱ	R-MMU-2172127 DAP12 interactions R-MMU-2424491 DAP12 signaling R-MMU-391160 Signal regulatory protein family interactions R-MMU-416700 Other semaphorin interactions R-MMU-6798695 Neutrophil degranulation

Reactomeⁱ

R-MMU-2172127 DAP12 interactions
R-MMU-2424491 DAP12 signaling
R-MMU-391160 Signal regulatory protein family interactions
R-MMU-416700 Other semaphorin interactions
R-MMU-6798695 Neutrophil degranulation

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:O54885
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000030579 Expressed in 231 organ(s), highest expression level in bone marrow
CleanExⁱ	MM_TYROBP
ExpressionAtlasⁱ	O54885 baseline and differential
Genevisibleⁱ	O54885 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR026200 Tyrobp
PANTHERⁱ	PTHR17554 PTHR17554, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TYOBP_MOUSE
Accessionⁱ	O54885Primary (citable) accession number: O54885 Secondary accession number(s): O88603
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
	Last sequence update:	June 1, 1998
	Last modified:	November 7, 2018
	This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - O54885 (TYOBP_MOUSE)

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TYRO protein tyrosine kinase-binding protein

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Plasma Membrane

Other locations

Topology

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ