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Protein

Testis-specific serine/threonine-protein kinase 2

Gene

Tssk2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Testis-specific serine/threonine-protein kinase required during spermatid development. Phosphorylates 'Ser-281' of TSKS and SPAG16. Involved in the late stages of spermatogenesis, during the reconstruction of the cytoplasm. During spermatogenesis, required for the transformation of a ring-shaped structure around the base of the flagellum originating from the chromatoid body.4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Mg2+1 PublicationNote: Mg2+ and Mn2+ were both present in the kinase buffer but Mg2+ is likely to be the in vivo cofactor.1 Publication

Activity regulationi

Activated by phosphorylation on Thr-174, potentially by autophosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei41ATPPROSITE-ProRule annotation1
Active sitei136Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 26ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • magnesium ion binding Source: UniProtKB
  • protein-containing complex binding Source: UniProtKB
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  • intracellular signal transduction Source: GO_Central
  • multicellular organism development Source: UniProtKB-KW
  • peptidyl-serine phosphorylation Source: UniProtKB
  • protein autophosphorylation Source: UniProtKB
  • protein phosphorylation Source: UniProtKB
  • spermatid development Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein, Kinase, Serine/threonine-protein kinase, Transferase
Biological processDifferentiation, Spermatogenesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Testis-specific serine/threonine-protein kinase 2 (EC:2.7.11.11 Publication)
Short name:
TSK-2
Short name:
TSK2
Short name:
TSSK-2
Short name:
Testis-specific kinase 2
Alternative name(s):
Serine/threonine-protein kinase 22B
Gene namesi
Name:Tssk2
Synonyms:Stk22b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1347559 Tssk2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

Male mice lacking Tssk1b and Tssk2 are sterile due to haploinsufficiency. chimeras show failure to form elongated spermatids, apoptosis of spermatocytes and spermatids, and the appearance of numerous round cells in the epididymal lumen. Elongating spermatids possess a collapsed mitochondrial sheath.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867691 – 358Testis-specific serine/threonine-protein kinase 2Add BLAST358

Post-translational modificationi

Autophosphorylated.By similarity
Ubiquitinated; HSP90 activity negatively regulates ubiquitination and degradation.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO54863
PRIDEiO54863

PTM databases

iPTMnetiO54863
PhosphoSitePlusiO54863

Expressioni

Tissue specificityi

Testis-specific. Expressed only in the spermatids postmeiotically at the final stages of cytodifferentiation in the seminiferous tubules (at protein level). Not detected in released sperms in the lumen of the seminiferous tubules. Also present in the epididymal sperm (at protein level).4 Publications

Developmental stagei

First expression detected at 3.5 to 4 weeks.

Gene expression databases

BgeeiENSMUSG00000045521 Expressed in 15 organ(s), highest expression level in testis
CleanExiMM_TSSK2
GenevisibleiO54863 MM

Interactioni

Subunit structurei

Interacts with TSSK1B (PubMed:10781952, PubMed:18367677). Interacts with HSP90; this interaction stabilizes TSSK2 (PubMed:23599433).3 Publications

Protein-protein interaction databases

IntActiO54863, 1 interactor
MINTiO54863
STRINGi10090.ENSMUSP00000051035

Structurei

3D structure databases

ProteinModelPortaliO54863
SMRiO54863
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 272Protein kinasePROSITE-ProRule annotationAdd BLAST261

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0583 Eukaryota
COG0515 LUCA
GeneTreeiENSGT00770000120521
HOGENOMiHOG000233016
HOVERGENiHBG101110
InParanoidiO54863
KOiK08811
OMAiEGKYRAE
OrthoDBiEOG091G0EZI
PhylomeDBiO54863
TreeFamiTF352374

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

O54863-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDDAAVLRKK GYIVGINLGK GSYAKVKSAY SERLKFNVAV KIIDRKKTPT
60 70 80 90 100
DFVERFLPRE MDILATVNHR SIIKTYEIFE TSDGRIYIVM ELGVQGDLLE
110 120 130 140 150
FIKCRGALHE DVARKMFRQL SSAVKYCHDL DVVHRDLKCE NLLLDKDFNI
160 170 180 190 200
KLSDFGFSKR CLRDGSGRIV LSKTFCGSAA YAAPEVLQGI PYQPKVYDIW
210 220 230 240 250
SLGVILYIMV CGSMPYDDSD IKKMLRIQKE HRVDFPRSKN LTGECKDLIY
260 270 280 290 300
RILQPDVNRR LHIDEILSHS WLQPPKPKAM SSASFKREGE GKYRADCKLD
310 320 330 340 350
TRPGSRPEHR PDHKLATKPQ QRMLVTPENE DRMEDRLAET SRAKDHHISG

AEVEKAST
Length:358
Mass (Da):41,022
Last modified:September 21, 2011 - v2
Checksum:i551F2AAEB0BBAF31
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti100E → T in AAC03367 (PubMed:9412477).Curated1
Sequence conflicti113 – 114AR → GG in AAC03367 (PubMed:9412477).Curated2
Sequence conflicti120L → V in AAC03367 (PubMed:9412477).Curated1
Sequence conflicti160R → G in AAC03367 (PubMed:9412477).Curated1
Sequence conflicti187L → R in AAC03367 (PubMed:9412477).Curated1
Sequence conflicti224Missing in AAC03367 (PubMed:9412477).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019926 mRNA Translation: AAC03367.1
BC061175 mRNA Translation: AAH61175.1
CH466521 Genomic DNA Translation: EDK97491.1
CCDSiCCDS28012.1
RefSeqiNP_033462.2, NM_009436.2
UniGeneiMm.310201

Genome annotation databases

EnsembliENSMUST00000055374; ENSMUSP00000051035; ENSMUSG00000045521
GeneIDi22115
KEGGimmu:22115
UCSCiuc007yml.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019926 mRNA Translation: AAC03367.1
BC061175 mRNA Translation: AAH61175.1
CH466521 Genomic DNA Translation: EDK97491.1
CCDSiCCDS28012.1
RefSeqiNP_033462.2, NM_009436.2
UniGeneiMm.310201

3D structure databases

ProteinModelPortaliO54863
SMRiO54863
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO54863, 1 interactor
MINTiO54863
STRINGi10090.ENSMUSP00000051035

PTM databases

iPTMnetiO54863
PhosphoSitePlusiO54863

Proteomic databases

PaxDbiO54863
PRIDEiO54863

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000055374; ENSMUSP00000051035; ENSMUSG00000045521
GeneIDi22115
KEGGimmu:22115
UCSCiuc007yml.1 mouse

Organism-specific databases

CTDi23617
MGIiMGI:1347559 Tssk2

Phylogenomic databases

eggNOGiKOG0583 Eukaryota
COG0515 LUCA
GeneTreeiENSGT00770000120521
HOGENOMiHOG000233016
HOVERGENiHBG101110
InParanoidiO54863
KOiK08811
OMAiEGKYRAE
OrthoDBiEOG091G0EZI
PhylomeDBiO54863
TreeFamiTF352374

Miscellaneous databases

PROiPR:O54863
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000045521 Expressed in 15 organ(s), highest expression level in testis
CleanExiMM_TSSK2
GenevisibleiO54863 MM

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTSSK2_MOUSE
AccessioniPrimary (citable) accession number: O54863
Secondary accession number(s): Q6P8M9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: September 21, 2011
Last modified: October 10, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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