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Protein

Choline kinase alpha

Gene

Chka

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline (By similarity).By similarity

Catalytic activityi

ATP + choline = ADP + phosphocholine.
ATP + ethanolamine = ADP + O-phosphoethanolamine.

Pathwayi: phosphatidylcholine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes phosphocholine from choline.
Proteins known to be involved in this subpathway in this organism are:
  1. Choline kinase alpha (Chka)
This subpathway is part of the pathway phosphatidylcholine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphocholine from choline, the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

Pathwayi: phosphatidylethanolamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes phosphatidylethanolamine from ethanolamine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Choline kinase alpha (Chka), Choline/ethanolamine kinase (Chkb), Ethanolamine kinase 2 (Etnk2), Ethanolamine kinase 1 (Etnk1)
  2. Ethanolamine-phosphate cytidylyltransferase (Pcyt2)
  3. Choline/ethanolaminephosphotransferase 1 (Cept1), Ethanolaminephosphotransferase 1 (Selenoi)
This subpathway is part of the pathway phosphatidylethanolamine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylethanolamine from ethanolamine, the pathway phosphatidylethanolamine biosynthesis and in Phospholipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei142ATPBy similarity1
Binding sitei304ATPBy similarity1
Binding sitei326ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi113 – 119ATPBy similarity7
Nucleotide bindingi203 – 209ATPBy similarity7

GO - Molecular functioni

  • ATP binding Source: MGI
  • choline binding Source: MGI
  • choline kinase activity Source: MGI
  • cholinesterase activity Source: MGI
  • drug binding Source: UniProtKB
  • ethanolamine kinase activity Source: UniProtKB
  • protein homodimerization activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1483191 Synthesis of PC
R-MMU-1483213 Synthesis of PE
UniPathwayi
UPA00558;UER00741

UPA00753;UER00737

Names & Taxonomyi

Protein namesi
Recommended name:
Choline kinase alpha (EC:2.7.1.32)
Short name:
CK
Alternative name(s):
CHETK-alpha
Ethanolamine kinase (EC:2.7.1.82)
Short name:
EK
Gene namesi
Name:Chka
Synonyms:Chk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:107760 Chka

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Death at an early embryonic stage. Embryos die after 3 to 5 days of development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002062201 – 453Choline kinase alphaAdd BLAST453

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei71PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO54804
PaxDbiO54804
PeptideAtlasiO54804
PRIDEiO54804

PTM databases

iPTMnetiO54804
PhosphoSitePlusiO54804

Expressioni

Tissue specificityi

Expressed ubiquitously with the highest level in testis.

Gene expression databases

BgeeiENSMUSG00000024843 Expressed in 265 organ(s), highest expression level in prostate gland ventral lobe
CleanExiMM_CHKA
ExpressionAtlasiO54804 baseline and differential
GenevisibleiO54804 MM

Interactioni

Subunit structurei

Homodimer, and heterodimer with CHKB.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000071933

Structurei

3D structure databases

ProteinModelPortaliO54804
SMRiO54804
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni115 – 117Substrate bindingBy similarity3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi53 – 81Pro-richAdd BLAST29

Sequence similaritiesi

Belongs to the choline/ethanolamine kinase family.Curated

Phylogenomic databases

eggNOGiKOG2686 Eukaryota
COG0510 LUCA
GeneTreeiENSGT00530000062991
HOGENOMiHOG000041274
HOVERGENiHBG050943
InParanoidiO54804
KOiK14156
OMAiLKERAHM
OrthoDBiEOG091G06UK
TreeFamiTF313549

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
SUPFAMiSSF56112 SSF56112, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O54804-1) [UniParc]FASTAAdd to basket
Also known as: CHETK-alpha2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MKTKFCTGGE AEPSPLGLLL SCGGNAAPTP GVGQQRDAAG ELESKQLGGR
60 70 80 90 100
TQPLALPPPP PPPLPLPPPP SPPLADEQPE PRTRRRAYLW CKEFLPGAWR
110 120 130 140 150
GLREDQFHIS VIRGGLSNML FQCSLPDSIA SVGDEPRKVL LRLYGAILKM
160 170 180 190 200
RSCNKEGSEQ AQNENEFQGA EAMVLESVMF AILAERSLGP KLFGIFPQGR
210 220 230 240 250
LEQFIPSRRL DTEELRLPDI SAEIAEKMAT FHGMKMPFNK EPKWLFGTME
260 270 280 290 300
KYLNQVLRLK FSREARVQQL HKILSYNLPL ELENLRSLLQ YTRSPVVFCH
310 320 330 340 350
NDCQEGNILL LEGQENSERR KLMLIDFEYS SYNYRGFDIG NHFCEWMYDY
360 370 380 390 400
TYEKYPFFRA NIQKYPSRKQ QLHFISSYLT TFQNDFESLS SEEQFATKED
410 420 430 440 450
MLLEVNRFAL ASHFLWGLWS IVQAKISSIE FGYMEYAQAR FEAYFDQKRK

LGV
Length:453
Mass (Da):51,985
Last modified:October 3, 2012 - v3
Checksum:i66042FEE92C333D4
GO
Isoform 2 (identifier: O54804-2) [UniParc]FASTAAdd to basket
Also known as: CHETK-alpha1

The sequence of this isoform differs from the canonical sequence as follows:
     151-168: Missing.

Show »
Length:435
Mass (Da):49,904
Checksum:iDC7377434296F68A
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5J2U5Q5J2U5_MOUSE
Choline kinase alpha
Chka mCG_3892
164Annotation score:
F7AYX1F7AYX1_MOUSE
Choline kinase alpha
Chka
163Annotation score:
F6S062F6S062_MOUSE
Choline kinase alpha
Chka
230Annotation score:
D6RG40D6RG40_MOUSE
Choline kinase alpha
Chka
76Annotation score:

Sequence cautioni

The sequence AAH56758 differs from that shown. Intron retention.Curated
The sequence BAA88154 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAB29191 differs from that shown. Intron retention.Curated
The sequence BAC34841 differs from that shown. Intron retention.Curated
The sequence BAC35539 differs from that shown. Intron retention.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti193F → Y in BAA24898 (PubMed:9714798).Curated1
Sequence conflicti193F → Y in BAA88154 (PubMed:10706593).Curated1
Sequence conflicti193F → Y in BAA88153 (PubMed:10706593).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009684151 – 168Missing in isoform 2. 2 PublicationsAdd BLAST18

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011002 mRNA Translation: BAA24898.1
AB030621 Genomic DNA Translation: BAA88153.1
AB030621 Genomic DNA Translation: BAA88154.1 Sequence problems.
AC133523 Genomic DNA No translation available.
CH466612 Genomic DNA Translation: EDL32956.1
BC056758 mRNA Translation: AAH56758.1 Sequence problems.
BC060218 mRNA No translation available.
AK014174 mRNA Translation: BAB29191.1 Sequence problems.
AK052056 mRNA Translation: BAC34841.1 Sequence problems.
AK053818 mRNA Translation: BAC35539.1 Sequence problems.
CCDSiCCDS29400.1 [O54804-2]
CCDS70913.1 [O54804-1]
RefSeqiNP_001258425.1, NM_001271496.1 [O54804-1]
NP_038518.2, NM_013490.4 [O54804-2]
UniGeneiMm.225505

Genome annotation databases

EnsembliENSMUST00000025760; ENSMUSP00000025760; ENSMUSG00000024843 [O54804-1]
ENSMUST00000072055; ENSMUSP00000071933; ENSMUSG00000024843 [O54804-2]
GeneIDi12660
KEGGimmu:12660
UCSCiuc008fxg.2 mouse [O54804-2]
uc008fxh.2 mouse [O54804-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011002 mRNA Translation: BAA24898.1
AB030621 Genomic DNA Translation: BAA88153.1
AB030621 Genomic DNA Translation: BAA88154.1 Sequence problems.
AC133523 Genomic DNA No translation available.
CH466612 Genomic DNA Translation: EDL32956.1
BC056758 mRNA Translation: AAH56758.1 Sequence problems.
BC060218 mRNA No translation available.
AK014174 mRNA Translation: BAB29191.1 Sequence problems.
AK052056 mRNA Translation: BAC34841.1 Sequence problems.
AK053818 mRNA Translation: BAC35539.1 Sequence problems.
CCDSiCCDS29400.1 [O54804-2]
CCDS70913.1 [O54804-1]
RefSeqiNP_001258425.1, NM_001271496.1 [O54804-1]
NP_038518.2, NM_013490.4 [O54804-2]
UniGeneiMm.225505

3D structure databases

ProteinModelPortaliO54804
SMRiO54804
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000071933

PTM databases

iPTMnetiO54804
PhosphoSitePlusiO54804

Proteomic databases

MaxQBiO54804
PaxDbiO54804
PeptideAtlasiO54804
PRIDEiO54804

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025760; ENSMUSP00000025760; ENSMUSG00000024843 [O54804-1]
ENSMUST00000072055; ENSMUSP00000071933; ENSMUSG00000024843 [O54804-2]
GeneIDi12660
KEGGimmu:12660
UCSCiuc008fxg.2 mouse [O54804-2]
uc008fxh.2 mouse [O54804-1]

Organism-specific databases

CTDi1119
MGIiMGI:107760 Chka

Phylogenomic databases

eggNOGiKOG2686 Eukaryota
COG0510 LUCA
GeneTreeiENSGT00530000062991
HOGENOMiHOG000041274
HOVERGENiHBG050943
InParanoidiO54804
KOiK14156
OMAiLKERAHM
OrthoDBiEOG091G06UK
TreeFamiTF313549

Enzyme and pathway databases

UniPathwayi
UPA00558;UER00741

UPA00753;UER00737

ReactomeiR-MMU-1483191 Synthesis of PC
R-MMU-1483213 Synthesis of PE

Miscellaneous databases

ChiTaRSiChka mouse
PROiPR:O54804
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024843 Expressed in 265 organ(s), highest expression level in prostate gland ventral lobe
CleanExiMM_CHKA
ExpressionAtlasiO54804 baseline and differential
GenevisibleiO54804 MM

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
SUPFAMiSSF56112 SSF56112, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCHKA_MOUSE
AccessioniPrimary (citable) accession number: O54804
Secondary accession number(s): G5E853
, Q8BPL3, Q8BPW6, Q9CXP3, Q9QX56
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: October 3, 2012
Last modified: November 7, 2018
This is version 136 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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