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Protein

Transcription factor MafG

Gene

Mafg

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed:16738329, PubMed:9679061). However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1 and NFE2L2, and recruiting them to specific DNA-binding sites (PubMed:16738329, PubMed:9679061). Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NFE2L2 transcription factor. Transcription factor, component of erythroid-specific transcription factor NFE2L2. Activates globin gene expression when associated with NFE2L2 (By similarity). May be involved in signal transduction of extracellular H+ (By similarity).By similarity

GO - Molecular functioni

  • DNA binding Source: MGI
  • protein heterodimerization activity Source: ParkinsonsUK-UCL
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB

GO - Biological processi

  • adult behavior Source: MGI
  • in utero embryonic development Source: MGI
  • positive regulation of gene expression Source: ParkinsonsUK-UCL
  • positive regulation of transcription by RNA polymerase II Source: NTNU_SB
  • regulation of cell proliferation Source: MGI
  • regulation of cellular pH Source: MGI
  • regulation of epidermal cell differentiation Source: MGI

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-983231 Factors involved in megakaryocyte development and platelet production

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor MafG
Alternative name(s):
V-maf musculoaponeurotic fibrosarcoma oncogene homolog G
Gene namesi
Name:Mafg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:96911 Mafg

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice, although viable and fertile, exhibit abnormal megakaryocyte proliferation as well as age-dependent behavioral defects. Megakaryocytes display both anti-glycoprotein IIb immunoreactivity and anti-acetylcholinesterase activity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000765011 – 162Transcription factor MafGAdd BLAST162

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki14Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate1 Publication
Cross-linki14Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei53N6-acetyllysineBy similarity1
Modified residuei60N6-acetyllysineBy similarity1
Modified residuei71N6-acetyllysineBy similarity1
Modified residuei76N6-acetyllysineBy similarity1
Modified residuei124PhosphoserineBy similarity1

Post-translational modificationi

Sumoylation at Lys-14 is required for active transcriptional repression.1 Publication
Acetylated in erythroid cells by CREB-binding protein (CBP). Acetylation augments the DNA-binding activity of NFE2, but has no effect on binding NFE2.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO54790
MaxQBiO54790
PaxDbiO54790
PeptideAtlasiO54790
PRIDEiO54790

PTM databases

iPTMnetiO54790
PhosphoSitePlusiO54790

Expressioni

Developmental stagei

Expressed throughout the embryo up until E8.5 with strong expression in the neural tube. Expression continues throughout the embryo with some intense expression also in the epithelium of the intestine, skeletal muscle, lens, retina, cranial nerve, and dorsal root ganglion cells. After birth, strong expression in the epidermis, hair follicles, epithelium of the digestive and respiratory tracts, and kidney tubules.1 Publication

Gene expression databases

BgeeiENSMUSG00000051510
GenevisibleiO54790 MM

Interactioni

Subunit structurei

Homodimer or heterodimer. Homodimerization leads to transcriptional repression. Forms high affinity heterodimers with members of the CNC-bZIP family such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3. Interacts with CREBBP; the interaction leads to acetylation of the basic region of MAFG and stimulation of NFE2 transcriptional activity through increased DNA binding.By similarity

GO - Molecular functioni

  • protein heterodimerization activity Source: ParkinsonsUK-UCL

Protein-protein interaction databases

BioGridi201283, 3 interactors
DIPiDIP-46344N
IntActiO54790, 3 interactors
MINTiO54790
STRINGi10090.ENSMUSP00000053899

Structurei

Secondary structure

1162
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi26 – 31Combined sources6
Helixi34 – 39Combined sources6
Turni40 – 43Combined sources4
Helixi46 – 83Combined sources38
Turni84 – 86Combined sources3
Turni89 – 94Combined sources6
Helixi99 – 102Combined sources4
Turni103 – 105Combined sources3
Beta strandi106 – 109Combined sources4

3D structure databases

ProteinModelPortaliO54790
SMRiO54790
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO54790

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini51 – 114bZIPPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni53 – 76Basic motifPROSITE-ProRule annotationAdd BLAST24
Regioni79 – 93Leucine-zipperPROSITE-ProRule annotationAdd BLAST15

Sequence similaritiesi

Belongs to the bZIP family. Maf subfamily.Curated

Phylogenomic databases

eggNOGiENOG410ISHW Eukaryota
ENOG4111HFT LUCA
GeneTreeiENSGT00550000074549
HOGENOMiHOG000045475
HOVERGENiHBG001725
InParanoidiO54790
KOiK09037
OMAiITASMGP
OrthoDBiEOG091G0H46
PhylomeDBiO54790
TreeFamiTF325689

Family and domain databases

InterProiView protein in InterPro
IPR004827 bZIP
IPR004826 bZIP_Maf
IPR028551 MafG
IPR008917 TF_DNA-bd_sf
IPR024874 Transciption_factor_Maf_fam
PANTHERiPTHR10129 PTHR10129, 1 hit
PTHR10129:SF15 PTHR10129:SF15, 1 hit
PfamiView protein in Pfam
PF03131 bZIP_Maf, 1 hit
SMARTiView protein in SMART
SM00338 BRLZ, 1 hit
SUPFAMiSSF47454 SSF47454, 1 hit
PROSITEiView protein in PROSITE
PS50217 BZIP, 1 hit

Sequencei

Sequence statusi: Complete.

O54790-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPNKGNKA LKVKREPGEN GTSLTDEELV TMSVRELNQH LRGLSKEEII
60 70 80 90 100
QLKQRRRTLK NRGYAASCRV KRVTQKEELE KQKAELQQEV EKLASENASM
110 120 130 140 150
KLELDALRSK YEALQNFART VARSPVAPAR GPLAAGLGPL VPGKVAATSV
160
ITIVKSKTDA RS
Length:162
Mass (Da):17,877
Last modified:June 1, 1998 - v1
Checksum:iC7FF19614EB95C7D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009693 Genomic DNA Translation: BAA24028.1
BC002092 mRNA Translation: AAH02092.1
BC052633 mRNA Translation: AAH52633.1
CCDSiCCDS25748.1
RefSeqiNP_034886.1, NM_010756.3
XP_017169797.1, XM_017314308.1
XP_017169798.1, XM_017314309.1
XP_017169799.1, XM_017314310.1
XP_017169800.1, XM_017314311.1
UniGeneiMm.268010
Mm.292957
Mm.393664

Genome annotation databases

EnsembliENSMUST00000058162; ENSMUSP00000053899; ENSMUSG00000051510
ENSMUST00000106180; ENSMUSP00000101786; ENSMUSG00000051510
ENSMUST00000106181; ENSMUSP00000101787; ENSMUSG00000051510
ENSMUST00000106182; ENSMUSP00000101788; ENSMUSG00000051510
GeneIDi17134
KEGGimmu:17134
UCSCiuc007mtu.2 mouse

Similar proteinsi

Entry informationi

Entry nameiMAFG_MOUSE
AccessioniPrimary (citable) accession number: O54790
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: June 20, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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