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Protein

DNA fragmentation factor subunit beta

Gene

Dffb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.

Activity regulationi

Inhibited by DFFA (DFF45).

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Nuclease
Biological processApoptosis

Enzyme and pathway databases

ReactomeiR-MMU-211227 Activation of DNA fragmentation factor

Names & Taxonomyi

Protein namesi
Recommended name:
DNA fragmentation factor subunit beta (EC:3.-.-.-)
Alternative name(s):
Caspase-activated deoxyribonuclease
Short name:
CAD
Short name:
Caspase-activated DNase
DNA fragmentation factor 40 kDa subunit
Short name:
DFF-40
Gene namesi
Name:Dffb
Synonyms:Cad
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1196287 Dffb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001447141 – 344DNA fragmentation factor subunit betaAdd BLAST344

Proteomic databases

MaxQBiO54788
PaxDbiO54788
PRIDEiO54788

PTM databases

PhosphoSitePlusiO54788

Expressioni

Gene expression databases

BgeeiENSMUSG00000029027 Expressed in 251 organ(s), highest expression level in thymus
CleanExiMM_DFFB
ExpressionAtlasiO54788 baseline and differential
GenevisibleiO54788 MM

Interactioni

Subunit structurei

Heterodimer of DFFA and DFFB. Interacts with HIST1H1A (By similarity).By similarity

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199222, 3 interactors
IntActiO54788, 3 interactors
MINTiO54788
STRINGi10090.ENSMUSP00000030893

Structurei

Secondary structure

1344
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO54788
SMRiO54788
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO54788

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 83CIDE-NPROSITE-ProRule annotationAdd BLAST77

Phylogenomic databases

eggNOGiENOG410IFTE Eukaryota
ENOG41102TS LUCA
GeneTreeiENSGT00390000014490
HOGENOMiHOG000006502
HOVERGENiHBG003828
InParanoidiO54788
KOiK02311
OMAiTRVRGYY
OrthoDBiEOG091G0BE7
PhylomeDBiO54788
TreeFamiTF102022

Family and domain databases

InterProiView protein in InterPro
IPR003508 CIDE-N_dom
IPR039729 DFF40
IPR015311 DFF40_C
PANTHERiPTHR13067 PTHR13067, 1 hit
PfamiView protein in Pfam
PF02017 CIDE-N, 1 hit
PF09230 DFF40, 1 hit
SMARTiView protein in SMART
SM00266 CAD, 1 hit
PROSITEiView protein in PROSITE
PS51135 CIDE_N, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O54788-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MCAVLRQPKC VKLRALHSAC KFGVAARSCQ ELLRKGCVRF QLPMPGSRLC
60 70 80 90 100
LYEDGTEVTD DCFPGLPNDA ELLLLTAGET WHGYVSDITR FLSVFNEPHA
110 120 130 140 150
GVIQAARQLL SDEQAPLRQK LLADLLHHVS QNITAETREQ DPSWFEGLES
160 170 180 190 200
RFRNKSGYLR YSCESRIRGY LREVSAYTSM VDEAAQEEYL RVLGSMCQKL
210 220 230 240 250
KSVQYNGSYF DRGAEASSRL CTPEGWFSCQ GPFDLESCLS KHSINPYGNR
260 270 280 290 300
ESRILFSTWN LDHIIEKKRT VVPTLAEAIQ DGREVNWEYF YSLLFTAENL
310 320 330 340
KLVHIACHKK TTHKLECDRS RIYRPQTGSR RKQPARKKRP ARKR
Length:344
Mass (Da):39,449
Last modified:June 1, 1998 - v1
Checksum:iE854B413EA139DE1
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1W2P7L3A0A1W2P7L3_MOUSE
DNA fragmentation factor subunit be...
Dffb
160Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009377 mRNA Translation: BAA24977.1
CCDSiCCDS19004.1
RefSeqiNP_031885.3, NM_007859.4
UniGeneiMm.388918

Genome annotation databases

EnsembliENSMUST00000030893; ENSMUSP00000030893; ENSMUSG00000029027
GeneIDi13368
KEGGimmu:13368
UCSCiuc008wav.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009377 mRNA Translation: BAA24977.1
CCDSiCCDS19004.1
RefSeqiNP_031885.3, NM_007859.4
UniGeneiMm.388918

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C9FNMR-A1-87[»]
1F2RNMR-C1-87[»]
1V0DX-ray2.60A1-329[»]
ProteinModelPortaliO54788
SMRiO54788
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199222, 3 interactors
IntActiO54788, 3 interactors
MINTiO54788
STRINGi10090.ENSMUSP00000030893

PTM databases

PhosphoSitePlusiO54788

Proteomic databases

MaxQBiO54788
PaxDbiO54788
PRIDEiO54788

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030893; ENSMUSP00000030893; ENSMUSG00000029027
GeneIDi13368
KEGGimmu:13368
UCSCiuc008wav.2 mouse

Organism-specific databases

CTDi1677
MGIiMGI:1196287 Dffb

Phylogenomic databases

eggNOGiENOG410IFTE Eukaryota
ENOG41102TS LUCA
GeneTreeiENSGT00390000014490
HOGENOMiHOG000006502
HOVERGENiHBG003828
InParanoidiO54788
KOiK02311
OMAiTRVRGYY
OrthoDBiEOG091G0BE7
PhylomeDBiO54788
TreeFamiTF102022

Enzyme and pathway databases

ReactomeiR-MMU-211227 Activation of DNA fragmentation factor

Miscellaneous databases

EvolutionaryTraceiO54788
PROiPR:O54788
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029027 Expressed in 251 organ(s), highest expression level in thymus
CleanExiMM_DFFB
ExpressionAtlasiO54788 baseline and differential
GenevisibleiO54788 MM

Family and domain databases

InterProiView protein in InterPro
IPR003508 CIDE-N_dom
IPR039729 DFF40
IPR015311 DFF40_C
PANTHERiPTHR13067 PTHR13067, 1 hit
PfamiView protein in Pfam
PF02017 CIDE-N, 1 hit
PF09230 DFF40, 1 hit
SMARTiView protein in SMART
SM00266 CAD, 1 hit
PROSITEiView protein in PROSITE
PS51135 CIDE_N, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDFFB_MOUSE
AccessioniPrimary (citable) accession number: O54788
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: June 1, 1998
Last modified: November 7, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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