UniProtKB - O54786 (DFFA_MOUSE)

BLAST

>sp|O54786|DFFA_MOUSE DNA fragmentation factor subunit alpha OS=Mus musculus OX=10090 GN=Dffa PE=1 SV=2
MELSRGASAPDPDDVRPLKPCLLRRNHSRDQHGVAASSLEELRSKACELLAIDKSLTPIT
LVLAEDGTIVDDDDYFLCLPSNTKFVALACNEKWIYNDSDGGTAWVSQESFEADEPDSRA
GVKWKNVARQLKEDLSSIILLSEEDLQALIDIPCAELAQELCQSCATVQGLQSTLQQVLD
QREEARQSKQLLELYLQALEKEGNILSNQKESKAALSEELDAVDTGVGREMASEVLLRSQ
ILTTLKEKPAPELSLSSQDLESVSKEDPKALAVALSWDIRKAETVQQACTTELALRLQQV
QSLHSLRNLSARRSPLPGEPQRPKRAKRDSS

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History
Entry version 158 (07 Nov 2018)
Sequence version 2 (27 Jul 2011)
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Protein

DNA fragmentation factor subunit alpha

Gene

Dffa

Organism

Mus musculus (Mouse)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Inhibitor of the caspase-activated DNase (DFF40).

GO - Molecular functionⁱ

deoxyribonuclease inhibitor activity Source: MGI
protein binding involved in protein folding Source: MGI
protein domain specific binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

apoptotic DNA fragmentation Source: InterPro
chaperone-mediated protein folding Source: MGI
negative regulation of apoptotic DNA fragmentation
Source: MGI
Inferred from mutant phenotypeⁱ
- 10601318
- 14663139
negative regulation of deoxyribonuclease activity Source: MGI
negative regulation of execution phase of apoptosis Source: MGI
positive regulation of apoptotic process
Source: MGI
Inferred from mutant phenotypeⁱ
- 14663139
thymocyte apoptotic process
Source: MGI
Inferred from mutant phenotypeⁱ
- 10601318

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Apoptosis

Biological process

Apoptosis

Enzyme and pathway databases

Reactomeⁱ	R-MMU-211227 Activation of DNA fragmentation factor

Reactomeⁱ

R-MMU-211227 Activation of DNA fragmentation factor

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: DNA fragmentation factor subunit alpha Alternative name(s): DNA fragmentation factor 45 kDa subunit Short name: DFF-45 Inhibitor of CAD Short name: ICAD
Gene namesⁱ	Name:Dffa Synonyms:Icad
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 4

Organism-specific databases

MGIⁱ	MGI:1196227 Dffa

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000144717	1 – 331	DNA fragmentation factor subunit alphaAdd BLAST		331

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	1	N-acetylmethionineBy similarity		1
Modified residueⁱ	243	PhosphothreonineBy similarity		1

Post-translational modificationⁱ

Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	117 – 118	Cleavage; by caspase-3By similarity		2
Siteⁱ	224 – 225	Cleavage; by caspase-3By similarity		2

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

EPDⁱ	O54786
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O54786
PaxDbⁱ	O54786
PeptideAtlas More...PeptideAtlasⁱ	O54786
PRIDEⁱ	O54786

PTM databases

iPTMnetⁱ	O54786
PhosphoSitePlusⁱ	O54786

Miscellaneous databases

PMAP-CutDBⁱ	O54786

PMAP-CutDBⁱ

O54786

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000028974 Expressed in 74 organ(s), highest expression level in soleus muscle
CleanExⁱ	MM_DFFA
ExpressionAtlasⁱ	O54786 baseline and differential
Genevisibleⁱ	O54786 MM

Interactionⁱ

Subunit structureⁱ

Heterodimer of DFFA and DFFB.

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Dffb	O54788	6	EBI-1634519,EBI-7365197

With

Entry

#Exp.

IntAct

Notes

Dffb

O54788

EBI-1634519,EBI-7365197

GO - Molecular functionⁱ

protein binding involved in protein folding Source: MGI
protein domain specific binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	199211, 2 interactors
IntActⁱ	O54786, 2 interactors
Molecular INTeraction database More...MINTⁱ	O54786
STRINGⁱ	10090.ENSMUSP00000030816

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	6 – 11	Combined sources	6
Beta strandⁱ	19 – 25	Combined sources	7
Beta strandⁱ	28 – 38	Combined sources	11
Helixⁱ	39 – 50	Combined sources	12
Helixⁱ	54 – 56	Combined sources	3
Beta strandⁱ	60 – 66	Combined sources	7
Beta strandⁱ	72 – 79	Combined sources	8
Beta strandⁱ	84 – 88	Combined sources	5
Beta strandⁱ	90 – 92	Combined sources	3

Show more details

3D structure databases

ProteinModelPortalⁱ	O54786
SMRⁱ	O54786
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	O54786

EvolutionaryTraceⁱ

O54786

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	17 – 96	CIDE-NPROSITE-ProRule annotationAdd BLAST		80

Phylogenomic databases

eggNOGⁱ	ENOG410IU5B Eukaryota ENOG4111HKY LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00510000048128
HOGENOMⁱ	HOG000112204
HOVERGENⁱ	HBG000683
InParanoidⁱ	O54786
KEGG Orthology (KO) More...KOⁱ	K02310
OMAⁱ	NWDIKKT
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G110A
TreeFamⁱ	TF102021

Family and domain databases

InterProⁱ	View protein in InterPro IPR003508 CIDE-N_dom IPR027296 DFF-C IPR017299 DFF45 IPR015121 DNA_fragmentation_mid_dom
PANTHERⁱ	PTHR12306:SF16 PTHR12306:SF16, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02017 CIDE-N, 1 hit PF09033 DFF-C, 1 hit
PIRSFⁱ	PIRSF037865 DFF_alpha, 1 hit
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD316494 DNA_fragmentation_C, 1 hit
SMARTⁱ	View protein in SMART SM00266 CAD, 1 hit
SUPFAMⁱ	SSF81783 SSF81783, 1 hit
PROSITEⁱ	View protein in PROSITE PS51135 CIDE_N, 1 hit

Sequences (2)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

Isoform ICAD-L (identifier: O54786-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MELSRGASAP DPDDVRPLKP CLLRRNHSRD QHGVAASSLE ELRSKACELL 
        60         70         80         90        100
AIDKSLTPIT LVLAEDGTIV DDDDYFLCLP SNTKFVALAC NEKWIYNDSD 
       110        120        130        140        150
GGTAWVSQES FEADEPDSRA GVKWKNVARQ LKEDLSSIIL LSEEDLQALI 
       160        170        180        190        200
DIPCAELAQE LCQSCATVQG LQSTLQQVLD QREEARQSKQ LLELYLQALE 
       210        220        230        240        250
KEGNILSNQK ESKAALSEEL DAVDTGVGRE MASEVLLRSQ ILTTLKEKPA 
       260        270        280        290        300
PELSLSSQDL ESVSKEDPKA LAVALSWDIR KAETVQQACT TELALRLQQV 
       310        320        330 
QSLHSLRNLS ARRSPLPGEP QRPKRAKRDS S

Length:331

Mass (Da):36,572

Last modified:July 27, 2011 - v2

Checksum:ⁱ4EA9314DD470040F

Isoform ICAD-S (identifier: O54786-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
262-265: SVSK → VGKN
266-331: Missing.

« Hide

        10         20         30         40         50
MELSRGASAP DPDDVRPLKP CLLRRNHSRD QHGVAASSLE ELRSKACELL 
        60         70         80         90        100
AIDKSLTPIT LVLAEDGTIV DDDDYFLCLP SNTKFVALAC NEKWIYNDSD 
       110        120        130        140        150
GGTAWVSQES FEADEPDSRA GVKWKNVARQ LKEDLSSIIL LSEEDLQALI 
       160        170        180        190        200
DIPCAELAQE LCQSCATVQG LQSTLQQVLD QREEARQSKQ LLELYLQALE 
       210        220        230        240        250
KEGNILSNQK ESKAALSEEL DAVDTGVGRE MASEVLLRSQ ILTTLKEKPA 
       260 
PELSLSSQDL EVGKN

Show »

Length:265

Mass (Da):29,183

Checksum:ⁱ588DCABE1E987912

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	95	I → T in BAA24140 (PubMed:9422506).Curated	1
Sequence conflictⁱ	95	I → T in BAA24141 (PubMed:9422513).Curated	1
Sequence conflictⁱ	95	I → T in CAQ52098 (PubMed:19468303).Curated	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_001087	262 – 265	SVSK → VGKN in isoform ICAD-S. Curated		4
Alternative sequenceⁱVSP_001088	266 – 331	Missing in isoform ICAD-S. CuratedAdd BLAST		66

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB009375 mRNA Translation: BAA24140.1 AB009376 mRNA Translation: BAA24141.1 AK051011 mRNA Translation: BAC34496.1 AL611967 Genomic DNA Translation: CAM13988.1 CU210839 Genomic DNA Translation: CAQ52098.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS18951.1 [O54786-1] CCDS18952.1 [O54786-2]
NCBI Reference Sequences More...RefSeqⁱ	NP_001020467.1, NM_001025296.2 [O54786-1]
UniGeneⁱ	Mm.41433

Genome annotation databases

Ensemblⁱ	ENSMUST00000030816; ENSMUSP00000030816; ENSMUSG00000028974 [O54786-1] ENSMUST00000103216; ENSMUSP00000099505; ENSMUSG00000028974 [O54786-2]
GeneIDⁱ	13347
KEGGⁱ	mmu:13347
UCSC genome browser More...UCSCⁱ	uc008vvr.2 mouse [O54786-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O54786	DNA fragmentation factor subunit alpha		RAT		328	UniRef90_O54786
DNAation factor, alpha subunit		MOUSE		265
UPI000A30F5AA		MUSPA		331
UPI000A318928		MUSCR		316
Isoform ICAD-S of DNA fragmentation factor subunit alpha		MOUSE		265
+3

Protein	Similar proteins		Species	Score	Length	Source
O54786	DNA fragmentation factor subunit alpha	)	HUMAN		331	UniRef50_O00273
DNAation factor, 45kDa, alpha polypeptide		PANTR		331
Uncharacterized protein		MANLE		331
Uncharacterized protein		MACFA		331
Uncharacterized protein		RHIRO		331
+193

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB009375 mRNA Translation: BAA24140.1 AB009376 mRNA Translation: BAA24141.1 AK051011 mRNA Translation: BAC34496.1 AL611967 Genomic DNA Translation: CAM13988.1 CU210839 Genomic DNA Translation: CAQ52098.1
CCDSⁱ	CCDS18951.1 [O54786-1] CCDS18952.1 [O54786-2]
RefSeqⁱ	NP_001020467.1, NM_001025296.2 [O54786-1]
UniGeneⁱ	Mm.41433

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1F2R	NMR	-	I	1-100	[»]
ProteinModelPortalⁱ	O54786
SMRⁱ	O54786
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	199211, 2 interactors
IntActⁱ	O54786, 2 interactors
MINTⁱ	O54786
STRINGⁱ	10090.ENSMUSP00000030816

PTM databases

iPTMnetⁱ	O54786
PhosphoSitePlusⁱ	O54786

Proteomic databases

EPDⁱ	O54786
MaxQBⁱ	O54786
PaxDbⁱ	O54786
PeptideAtlasⁱ	O54786
PRIDEⁱ	O54786

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000030816; ENSMUSP00000030816; ENSMUSG00000028974 [O54786-1] ENSMUST00000103216; ENSMUSP00000099505; ENSMUSG00000028974 [O54786-2]
GeneIDⁱ	13347
KEGGⁱ	mmu:13347
UCSCⁱ	uc008vvr.2 mouse [O54786-1]

Organism-specific databases

CTDⁱ	1676
MGIⁱ	MGI:1196227 Dffa

Phylogenomic databases

eggNOGⁱ	ENOG410IU5B Eukaryota ENOG4111HKY LUCA
GeneTreeⁱ	ENSGT00510000048128
HOGENOMⁱ	HOG000112204
HOVERGENⁱ	HBG000683
InParanoidⁱ	O54786
KOⁱ	K02310
OMAⁱ	NWDIKKT
OrthoDBⁱ	EOG091G110A
TreeFamⁱ	TF102021

Enzyme and pathway databases

Reactomeⁱ	R-MMU-211227 Activation of DNA fragmentation factor

Reactomeⁱ

R-MMU-211227 Activation of DNA fragmentation factor

Miscellaneous databases

ChiTaRSⁱ	Dffa mouse
EvolutionaryTraceⁱ	O54786
PMAP-CutDBⁱ	O54786
Protein Ontology More...PROⁱ	PR:O54786
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000028974 Expressed in 74 organ(s), highest expression level in soleus muscle
CleanExⁱ	MM_DFFA
ExpressionAtlasⁱ	O54786 baseline and differential
Genevisibleⁱ	O54786 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR003508 CIDE-N_dom IPR027296 DFF-C IPR017299 DFF45 IPR015121 DNA_fragmentation_mid_dom
PANTHERⁱ	PTHR12306:SF16 PTHR12306:SF16, 1 hit
Pfamⁱ	View protein in Pfam PF02017 CIDE-N, 1 hit PF09033 DFF-C, 1 hit
PIRSFⁱ	PIRSF037865 DFF_alpha, 1 hit
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD316494 DNA_fragmentation_C, 1 hit
SMARTⁱ	View protein in SMART SM00266 CAD, 1 hit
SUPFAMⁱ	SSF81783 SSF81783, 1 hit
PROSITEⁱ	View protein in PROSITE PS51135 CIDE_N, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	DFFA_MOUSE
Accessionⁱ	O54786Primary (citable) accession number: O54786 Secondary accession number(s): B2KFX0, O54787, Q8BQC7
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
	Last sequence update:	July 27, 2011
	Last modified:	November 7, 2018
	This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

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UniParc

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Supporting data

UniProtKB - O54786 (DFFA_MOUSE)

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DNA fragmentation factor subunit alpha

Dffa

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Cytosol

Nucleus

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Sites

Keywords - PTMi

Proteomic databases

PTM databases

Miscellaneous databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Phylogenomic databases

Family and domain databases

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ