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UniProtKB - O54786 (DFFA_MOUSE)
Protein
DNA fragmentation factor subunit alpha
Gene
Dffa
Organism
Mus musculus (Mouse)
Status
Functioni
Inhibitor of the caspase-activated DNase (DFF40).
GO - Molecular functioni
- deoxyribonuclease inhibitor activity Source: MGI
- protein domain specific binding Source: MGI
- protein folding chaperone Source: MGI
GO - Biological processi
- apoptotic DNA fragmentation Source: InterPro
- chaperone-mediated protein folding Source: MGI
- negative regulation of apoptotic DNA fragmentation Source: MGI
- negative regulation of deoxyribonuclease activity Source: MGI
- negative regulation of execution phase of apoptosis Source: MGI
- positive regulation of apoptotic process Source: MGI
- regulation of apoptotic process Source: GO_Central
- thymocyte apoptotic process Source: MGI
Keywordsi
Biological process | Apoptosis |
Enzyme and pathway databases
Reactomei | R-MMU-140342, Apoptosis induced DNA fragmentation |
Names & Taxonomyi
Protein namesi | Recommended name: DNA fragmentation factor subunit alphaAlternative name(s): DNA fragmentation factor 45 kDa subunit Short name: DFF-45 Inhibitor of CAD Short name: ICAD |
Gene namesi | Name:Dffa Synonyms:Icad |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1196227, Dffa |
VEuPathDBi | HostDB:ENSMUSG00000028974 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Cytosol
- cytosol Source: MGI
Nucleus
- nucleus Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- chromatin Source: MGI
- protein-containing complex Source: MGI
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000144717 | 1 – 331 | DNA fragmentation factor subunit alphaAdd BLAST | 331 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 243 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 117 – 118 | Cleavage; by caspase-3By similarity | 2 | |
Sitei | 224 – 225 | Cleavage; by caspase-3By similarity | 2 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | O54786 |
jPOSTi | O54786 |
MaxQBi | O54786 |
PaxDbi | O54786 |
PeptideAtlasi | O54786 |
PRIDEi | O54786 |
ProteomicsDBi | 279409 [O54786-1] 279410 [O54786-2] |
PTM databases
iPTMneti | O54786 |
PhosphoSitePlusi | O54786 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000028974, Expressed in soleus muscle and 119 other tissues |
ExpressionAtlasi | O54786, baseline and differential |
Genevisiblei | O54786, MM |
Interactioni
Subunit structurei
Heterodimer of DFFA and DFFB.
By similarityBinary interactionsi
O54786
With | #Exp. | IntAct |
---|---|---|
Dffb [O54788] | 7 | EBI-1634519,EBI-7365197 |
GO - Molecular functioni
- protein domain specific binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 199211, 17 interactors |
IntActi | O54786, 4 interactors |
MINTi | O54786 |
STRINGi | 10090.ENSMUSP00000030816 |
Miscellaneous databases
RNActi | O54786, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O54786 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O54786 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 17 – 96 | CIDE-NPROSITE-ProRule annotationAdd BLAST | 80 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 306 – 331 | DisorderedSequence analysisAdd BLAST | 26 |
Phylogenomic databases
eggNOGi | ENOG502RQ19, Eukaryota |
GeneTreei | ENSGT00390000018596 |
HOGENOMi | CLU_086234_0_0_1 |
InParanoidi | O54786 |
OMAi | NWDIRKT |
OrthoDBi | 1564672at2759 |
PhylomeDBi | O54786 |
TreeFami | TF102021 |
Family and domain databases
InterProi | View protein in InterPro IPR003508, CIDE-N_dom IPR027296, DFF-C IPR017299, DFF45 IPR015121, DNA_fragmentation_mid_dom |
PANTHERi | PTHR12306:SF16, PTHR12306:SF16, 1 hit |
Pfami | View protein in Pfam PF02017, CIDE-N, 1 hit PF09033, DFF-C, 1 hit |
PIRSFi | PIRSF037865, DFF_alpha, 1 hit |
SMARTi | View protein in SMART SM00266, CAD, 1 hit |
SUPFAMi | SSF81783, SSF81783, 1 hit |
PROSITEi | View protein in PROSITE PS51135, CIDE_N, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform ICAD-L (identifier: O54786-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MELSRGASAP DPDDVRPLKP CLLRRNHSRD QHGVAASSLE ELRSKACELL
60 70 80 90 100
AIDKSLTPIT LVLAEDGTIV DDDDYFLCLP SNTKFVALAC NEKWIYNDSD
110 120 130 140 150
GGTAWVSQES FEADEPDSRA GVKWKNVARQ LKEDLSSIIL LSEEDLQALI
160 170 180 190 200
DIPCAELAQE LCQSCATVQG LQSTLQQVLD QREEARQSKQ LLELYLQALE
210 220 230 240 250
KEGNILSNQK ESKAALSEEL DAVDTGVGRE MASEVLLRSQ ILTTLKEKPA
260 270 280 290 300
PELSLSSQDL ESVSKEDPKA LAVALSWDIR KAETVQQACT TELALRLQQV
310 320 330
QSLHSLRNLS ARRSPLPGEP QRPKRAKRDS S
Isoform ICAD-S (identifier: O54786-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
262-265: SVSK → VGKN
266-331: Missing.
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 95 | I → T in BAA24140 (PubMed:9422506).Curated | 1 | |
Sequence conflicti | 95 | I → T in BAA24141 (PubMed:9422513).Curated | 1 | |
Sequence conflicti | 95 | I → T in CAQ52098 (PubMed:19468303).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_001087 | 262 – 265 | SVSK → VGKN in isoform ICAD-S. Curated | 4 | |
Alternative sequenceiVSP_001088 | 266 – 331 | Missing in isoform ICAD-S. CuratedAdd BLAST | 66 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB009375 mRNA Translation: BAA24140.1 AB009376 mRNA Translation: BAA24141.1 AK051011 mRNA Translation: BAC34496.1 AL611967 Genomic DNA Translation: CAM13988.1 CU210839 Genomic DNA Translation: CAQ52098.1 |
CCDSi | CCDS18951.1 [O54786-1] CCDS18952.1 [O54786-2] |
RefSeqi | NP_001020467.1, NM_001025296.2 [O54786-1] |
Genome annotation databases
Ensembli | ENSMUST00000030816; ENSMUSP00000030816; ENSMUSG00000028974 [O54786-1] ENSMUST00000103216; ENSMUSP00000099505; ENSMUSG00000028974 [O54786-2] |
GeneIDi | 13347 |
KEGGi | mmu:13347 |
UCSCi | uc008vvr.2, mouse [O54786-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB009375 mRNA Translation: BAA24140.1 AB009376 mRNA Translation: BAA24141.1 AK051011 mRNA Translation: BAC34496.1 AL611967 Genomic DNA Translation: CAM13988.1 CU210839 Genomic DNA Translation: CAQ52098.1 |
CCDSi | CCDS18951.1 [O54786-1] CCDS18952.1 [O54786-2] |
RefSeqi | NP_001020467.1, NM_001025296.2 [O54786-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1F2R | NMR | - | I | 1-100 | [»] | |
SMRi | O54786 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 199211, 17 interactors |
IntActi | O54786, 4 interactors |
MINTi | O54786 |
STRINGi | 10090.ENSMUSP00000030816 |
PTM databases
iPTMneti | O54786 |
PhosphoSitePlusi | O54786 |
Proteomic databases
EPDi | O54786 |
jPOSTi | O54786 |
MaxQBi | O54786 |
PaxDbi | O54786 |
PeptideAtlasi | O54786 |
PRIDEi | O54786 |
ProteomicsDBi | 279409 [O54786-1] 279410 [O54786-2] |
Protocols and materials databases
Antibodypediai | 3633, 834 antibodies from 45 providers |
DNASUi | 13347 |
Genome annotation databases
Ensembli | ENSMUST00000030816; ENSMUSP00000030816; ENSMUSG00000028974 [O54786-1] ENSMUST00000103216; ENSMUSP00000099505; ENSMUSG00000028974 [O54786-2] |
GeneIDi | 13347 |
KEGGi | mmu:13347 |
UCSCi | uc008vvr.2, mouse [O54786-1] |
Organism-specific databases
CTDi | 1676 |
MGIi | MGI:1196227, Dffa |
VEuPathDBi | HostDB:ENSMUSG00000028974 |
Phylogenomic databases
eggNOGi | ENOG502RQ19, Eukaryota |
GeneTreei | ENSGT00390000018596 |
HOGENOMi | CLU_086234_0_0_1 |
InParanoidi | O54786 |
OMAi | NWDIRKT |
OrthoDBi | 1564672at2759 |
PhylomeDBi | O54786 |
TreeFami | TF102021 |
Enzyme and pathway databases
Reactomei | R-MMU-140342, Apoptosis induced DNA fragmentation |
Miscellaneous databases
BioGRID-ORCSi | 13347, 1 hit in 65 CRISPR screens |
ChiTaRSi | Dffa, mouse |
EvolutionaryTracei | O54786 |
PROi | PR:O54786 |
RNActi | O54786, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000028974, Expressed in soleus muscle and 119 other tissues |
ExpressionAtlasi | O54786, baseline and differential |
Genevisiblei | O54786, MM |
Family and domain databases
InterProi | View protein in InterPro IPR003508, CIDE-N_dom IPR027296, DFF-C IPR017299, DFF45 IPR015121, DNA_fragmentation_mid_dom |
PANTHERi | PTHR12306:SF16, PTHR12306:SF16, 1 hit |
Pfami | View protein in Pfam PF02017, CIDE-N, 1 hit PF09033, DFF-C, 1 hit |
PIRSFi | PIRSF037865, DFF_alpha, 1 hit |
SMARTi | View protein in SMART SM00266, CAD, 1 hit |
SUPFAMi | SSF81783, SSF81783, 1 hit |
PROSITEi | View protein in PROSITE PS51135, CIDE_N, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DFFA_MOUSE | |
Accessioni | O54786Primary (citable) accession number: O54786 Secondary accession number(s): B2KFX0, O54787, Q8BQC7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 23, 2022 | |
This is version 174 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references