UniProtKB - O54785 (LIMK2_MOUSE)
Protein
LIM domain kinase 2
Gene
Limk2
Organism
Mus musculus (Mouse)
Status
Functioni
Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.By similarity
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 360 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 451 | By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 337 – 345 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- protein heterodimerization activity Source: MGI
- protein kinase activity Source: MGI
- protein serine/threonine kinase activity Source: MGI
GO - Biological processi
- actin cytoskeleton organization Source: GO_Central
- cornea development in camera-type eye Source: MGI
- head development Source: MGI
- protein phosphorylation Source: MGI
- spermatogenesis Source: MGI
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Limk2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1197517 Limk2 |
Subcellular locationi
Other locations
Golgi apparatus
- cis-Golgi network Source: MGI
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000075810 | 1 – 638 | LIM domain kinase 2Add BLAST | 638 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 210 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 293 | PhosphoserineBy similarity | 1 | |
Modified residuei | 298 | PhosphoserineBy similarity | 1 | |
Modified residuei | 505 | Phosphothreonine; by ROCK1 and CDC42BPBy similarity | 1 |
Post-translational modificationi
Phosphorylated on serine and/or threonine residues by ROCK1.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | O54785 |
PeptideAtlasi | O54785 |
PRIDEi | O54785 |
PTM databases
iPTMneti | O54785 |
PhosphoSitePlusi | O54785 |
Expressioni
Tissue specificityi
Isoform 3 is testis specific.
Gene expression databases
Bgeei | ENSMUSG00000020451 Expressed in 284 organ(s), highest expression level in placenta |
ExpressionAtlasi | O54785 baseline and differential |
Genevisiblei | O54785 MM |
Interactioni
Subunit structurei
Binds ROCK1 and MARF1 (By similarity). Interacts with PARD3 (By similarity). Interacts with NISCH.By similarity1 Publication
GO - Molecular functioni
- protein heterodimerization activity Source: MGI
Protein-protein interaction databases
BioGridi | 201167, 2 interactors |
STRINGi | 10090.ENSMUSP00000099162 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2YUB | NMR | - | A | 142-246 | [»] | |
ProteinModelPortali | O54785 | |||||
SMRi | O54785 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O54785 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 12 – 63 | LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST | 52 | |
Domaini | 72 – 124 | LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST | 53 | |
Domaini | 152 – 239 | PDZPROSITE-ProRule annotationAdd BLAST | 88 | |
Domaini | 331 – 608 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 278 |
Sequence similaritiesi
Keywords - Domaini
LIM domain, RepeatPhylogenomic databases
eggNOGi | ENOG410IEZ9 Eukaryota COG0515 LUCA |
GeneTreei | ENSGT00940000159133 |
HOGENOMi | HOG000013121 |
HOVERGENi | HBG052328 |
InParanoidi | O54785 |
KOi | K05744 |
OrthoDBi | 988822at2759 |
PhylomeDBi | O54785 |
TreeFami | TF318014 |
Family and domain databases
InterProi | View protein in InterPro IPR011009 Kinase-like_dom_sf IPR001478 PDZ IPR036034 PDZ_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR001245 Ser-Thr/Tyr_kinase_cat_dom IPR001781 Znf_LIM |
Pfami | View protein in Pfam PF00412 LIM, 2 hits PF00595 PDZ, 1 hit PF07714 Pkinase_Tyr, 1 hit |
SMARTi | View protein in SMART SM00132 LIM, 2 hits SM00228 PDZ, 1 hit |
SUPFAMi | SSF50156 SSF50156, 1 hit SSF56112 SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00478 LIM_DOMAIN_1, 2 hits PS50023 LIM_DOMAIN_2, 2 hits PS50106 PDZ, 1 hit PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: O54785-1) [UniParc]FASTAAdd to basket
Also known as: LIMK2a
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MAALAGDEAW RCRGCGTYVP LSQRLYRTAN EAWHGSCFRC SECQESLTNW
60 70 80 90 100
YYEKDGKLYC HKDYWAKFGE FCHGCSLLMT GPAMVAGEFK YHPECFACMS
110 120 130 140 150
CKVIIEDGDA YALVQHATLY CGKCHNEVVL APMFERLSTE SVQDQLPYSV
160 170 180 190 200
TLISMPATTE CRRGFSVTVE SASSNYATTV QVKEVNRMHI SPNNRNAIHP
210 220 230 240 250
GDRILEINGT PVRTLRVEEV EDAIKQTSQT LQLLIEHDPV PQRLDQLRLD
260 270 280 290 300
ARLPPHMQST GHTLMLSTLD TKENQEGTLR RRSLRRSNSI SKSPGPSSPK
310 320 330 340 350
EPLLLSRDIS RSESLRCSSS YSQQIFRPCD LIHGEVLGKG FFGQAIKVTH
360 370 380 390 400
KATGKVMVMK ELIRCDEETQ KTFLTEVKVM RSLDHPNVLK FIGVLYKDKK
410 420 430 440 450
LNLLTEYIEG GTLKDFLRSV DPFPWQQKVR FAKGISSGMA YLHSMCIIHR
460 470 480 490 500
DLNSHNCLIK LDKTVVVADF GLSRLIVEER KRPPVEKATT KKRTLRKSDR
510 520 530 540 550
KKRYTVVGNP YWMAPEMLNG KSYDETVDVF SFGIVLCEII GQVYADPDCL
560 570 580 590 600
PRTLDFGLNV KLFWEKFVPT DCPPAFFPLA AICCKLEPES RPAFSKLEDS
610 620 630
FEALSLFLGE LAIPLPAELE DLDHTVSMEY GLTRDSPP
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ5NC03 | Q5NC03_MOUSE | LIM domain kinase 2 | Limk2 | 623 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_010351 | 1 – 187 | Missing in isoform 3. 2 PublicationsAdd BLAST | 187 | |
Alternative sequenceiVSP_010350 | 1 – 37 | MAALA…WHGSC → MGSYLSVPAYFTSRDP in isoform 2. 1 PublicationAdd BLAST | 37 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB008117 mRNA Translation: BAA29035.1 AB005140 Genomic DNA Translation: BAA24491.1 AB005131 mRNA Translation: BAA24488.1 AB005132 mRNA Translation: BAA24489.1 AB005134 Genomic DNA Translation: BAA24490.1 U88618 mRNA Translation: AAC39947.1 AB012291 mRNA Translation: BAA32437.1 AB012092 mRNA Translation: BAA31147.1 BC007129 mRNA Translation: AAH07129.1 |
CCDSi | CCDS24358.1 [O54785-1] CCDS24359.1 [O54785-2] CCDS24360.1 [O54785-3] |
PIRi | JC5813 JC5814 JE0240 |
RefSeqi | NP_001029202.1, NM_001034030.2 [O54785-3] NP_034848.1, NM_010718.4 [O54785-1] NP_774958.1, NM_173053.1 [O54785-2] |
UniGenei | Mm.124176 Mm.390323 Mm.442189 |
Genome annotation databases
Ensembli | ENSMUST00000101638; ENSMUSP00000099162; ENSMUSG00000020451 [O54785-1] ENSMUST00000101642; ENSMUSP00000099165; ENSMUSG00000020451 [O54785-2] ENSMUST00000110029; ENSMUSP00000105656; ENSMUSG00000020451 [O54785-3] |
GeneIDi | 16886 |
KEGGi | mmu:16886 |
UCSCi | uc007hss.1 mouse [O54785-1] uc007hst.1 mouse [O54785-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB008117 mRNA Translation: BAA29035.1 AB005140 Genomic DNA Translation: BAA24491.1 AB005131 mRNA Translation: BAA24488.1 AB005132 mRNA Translation: BAA24489.1 AB005134 Genomic DNA Translation: BAA24490.1 U88618 mRNA Translation: AAC39947.1 AB012291 mRNA Translation: BAA32437.1 AB012092 mRNA Translation: BAA31147.1 BC007129 mRNA Translation: AAH07129.1 |
CCDSi | CCDS24358.1 [O54785-1] CCDS24359.1 [O54785-2] CCDS24360.1 [O54785-3] |
PIRi | JC5813 JC5814 JE0240 |
RefSeqi | NP_001029202.1, NM_001034030.2 [O54785-3] NP_034848.1, NM_010718.4 [O54785-1] NP_774958.1, NM_173053.1 [O54785-2] |
UniGenei | Mm.124176 Mm.390323 Mm.442189 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2YUB | NMR | - | A | 142-246 | [»] | |
ProteinModelPortali | O54785 | |||||
SMRi | O54785 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Protein-protein interaction databases
BioGridi | 201167, 2 interactors |
STRINGi | 10090.ENSMUSP00000099162 |
PTM databases
iPTMneti | O54785 |
PhosphoSitePlusi | O54785 |
Proteomic databases
PaxDbi | O54785 |
PeptideAtlasi | O54785 |
PRIDEi | O54785 |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Genome annotation databases
Ensembli | ENSMUST00000101638; ENSMUSP00000099162; ENSMUSG00000020451 [O54785-1] ENSMUST00000101642; ENSMUSP00000099165; ENSMUSG00000020451 [O54785-2] ENSMUST00000110029; ENSMUSP00000105656; ENSMUSG00000020451 [O54785-3] |
GeneIDi | 16886 |
KEGGi | mmu:16886 |
UCSCi | uc007hss.1 mouse [O54785-1] uc007hst.1 mouse [O54785-2] |
Organism-specific databases
CTDi | 3985 |
MGIi | MGI:1197517 Limk2 |
Phylogenomic databases
eggNOGi | ENOG410IEZ9 Eukaryota COG0515 LUCA |
GeneTreei | ENSGT00940000159133 |
HOGENOMi | HOG000013121 |
HOVERGENi | HBG052328 |
InParanoidi | O54785 |
KOi | K05744 |
OrthoDBi | 988822at2759 |
PhylomeDBi | O54785 |
TreeFami | TF318014 |
Miscellaneous databases
EvolutionaryTracei | O54785 |
PROi | PR:O54785 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020451 Expressed in 284 organ(s), highest expression level in placenta |
ExpressionAtlasi | O54785 baseline and differential |
Genevisiblei | O54785 MM |
Family and domain databases
InterProi | View protein in InterPro IPR011009 Kinase-like_dom_sf IPR001478 PDZ IPR036034 PDZ_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR001245 Ser-Thr/Tyr_kinase_cat_dom IPR001781 Znf_LIM |
Pfami | View protein in Pfam PF00412 LIM, 2 hits PF00595 PDZ, 1 hit PF07714 Pkinase_Tyr, 1 hit |
SMARTi | View protein in SMART SM00132 LIM, 2 hits SM00228 PDZ, 1 hit |
SUPFAMi | SSF50156 SSF50156, 1 hit SSF56112 SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00478 LIM_DOMAIN_1, 2 hits PS50023 LIM_DOMAIN_2, 2 hits PS50106 PDZ, 1 hit PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | LIMK2_MOUSE | |
Accessioni | O54785Primary (citable) accession number: O54785 Secondary accession number(s): O54776, O55238, Q9QUL4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 24, 2004 |
Last sequence update: | August 1, 1998 | |
Last modified: | February 13, 2019 | |
This is version 159 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - SIMILARITY comments
Index of protein domains and families - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot