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Protein

Choline/ethanolamine kinase

Gene

Chkb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a key role in phospholipid biosynthesis. Catalyzes the first step in phosphatidylethanolamine biosynthesis. Phosphorylates ethanolamine, and can also act on choline (in vitro). Has higher activity with ethanolamine. May not significantly contribute to in vivo phosphatidylcholine biosynthesis (By similarity).By similarity

Catalytic activityi

ATP + choline = ADP + phosphocholine.
ATP + ethanolamine = ADP + O-phosphoethanolamine.

Pathwayi: phosphatidylethanolamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes phosphatidylethanolamine from ethanolamine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Choline kinase alpha (Chka), Choline/ethanolamine kinase (Chkb), Ethanolamine kinase 2 (Etnk2)
  2. Ethanolamine-phosphate cytidylyltransferase (Pcyt2)
  3. Choline/ethanolaminephosphotransferase 1 (Cept1)
This subpathway is part of the pathway phosphatidylethanolamine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylethanolamine from ethanolamine, the pathway phosphatidylethanolamine biosynthesis and in Phospholipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei104ATPBy similarity1
Binding sitei244ATPBy similarity1
Binding sitei264ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi75 – 81ATPBy similarity7
Nucleotide bindingi146 – 152ATPBy similarity7

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • choline kinase activity Source: RGD
  • ethanolamine kinase activity Source: UniProtKB-EC

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-1483191 Synthesis of PC
R-RNO-1483213 Synthesis of PE
SABIO-RKiO54783
UniPathwayiUPA00558; UER00741

Names & Taxonomyi

Protein namesi
Recommended name:
Choline/ethanolamine kinase
Alternative name(s):
Choline kinase beta (EC:2.7.1.32)
Short name:
CK
Short name:
CKB
Ethanolamine kinase (EC:2.7.1.82)
Short name:
EK
choline/ethanolamine kinase beta
Short name:
CKEKB
Gene namesi
Name:Chkb
Synonyms:Chetk, Chkl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi61826 Chkb

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002062242 – 394Choline/ethanolamine kinaseAdd BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO54783
PRIDEiO54783

Expressioni

Gene expression databases

BgeeiENSRNOG00000011404
GenevisibleiO54783 RN

Interactioni

Subunit structurei

Homodimer, and heterodimer with CHKA.By similarity

Protein-protein interaction databases

BioGridi248021, 1 interactor
STRINGi10116.ENSRNOP00000015374

Structurei

3D structure databases

ProteinModelPortaliO54783
SMRiO54783
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni77 – 79Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the choline/ethanolamine kinase family.Curated

Phylogenomic databases

eggNOGiKOG2686 Eukaryota
COG0510 LUCA
GeneTreeiENSGT00530000062991
HOGENOMiHOG000041274
HOVERGENiHBG050943
InParanoidiO54783
KOiK14156
OMAiREQQLHF
OrthoDBiEOG091G06UK
PhylomeDBiO54783
TreeFamiTF313549

Family and domain databases

InterProiView protein in InterPro
IPR026712 Cho/Etha_kinase
IPR011009 Kinase-like_dom_sf
PANTHERiPTHR22603:SF35 PTHR22603:SF35, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O54783-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAADGTGVVG GGAVGGPLSK DGLLDAKCPE PIPNRRRSSS LSRDAQRRAY
60 70 80 90 100
QWCREYLGGA WRRARPEELS VCPVSGGLSN LLFRCSLPNH VPSMGGEPRE
110 120 130 140 150
VLLRLYGAIL QGVDSLVLES VMFAILAERS LGPQLYGVFP EGRLEQYLPS
160 170 180 190 200
RPLKTQELRD PVLSGAIATK MARFHGMEMP FTKEPRWLFG TMERYLKQIQ
210 220 230 240 250
DLPSTSLPQM NLVEMYSLKD EMNHLRTLLD ATPSPVVFCH NDIQEGNILL
260 270 280 290 300
LSEPDSDDNL MLVDFEYSSY NYRGFDIGNH FCEWVYDYTY EEWPFYKARP
310 320 330 340 350
ADYPTREQQL LFIRHYLAEV QKGEVLSEEE QKKQEEDLLI EISRYALASH
360 370 380 390
FFWGLWSTLQ ASMSTIEFGY LEYAQSRFQF YFQQKGQLTS FLSP
Length:394
Mass (Da):45,100
Last modified:January 23, 2007 - v3
Checksum:iA2E99EB055E3959E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006607 mRNA Translation: BAA24366.1
BC060515 mRNA Translation: AAH60515.1
RefSeqiNP_058873.1, NM_017177.1
UniGeneiRn.1302

Genome annotation databases

EnsembliENSRNOT00000015374; ENSRNOP00000015374; ENSRNOG00000011404
GeneIDi29367
KEGGirno:29367

Similar proteinsi

Entry informationi

Entry nameiCHKB_RAT
AccessioniPrimary (citable) accession number: O54783
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 115 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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