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Protein

Epididymis-specific alpha-mannosidase

Gene

Man2b2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an important role in the early step of spermatogenesis.

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi36ZincBy similarity1
Metal bindingi38ZincBy similarity1
Active sitei151NucleophileBy similarity1
Metal bindingi151ZincBy similarity1
Metal bindingi420ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

  • mannose metabolic process Source: MGI
  • protein deglycosylation Source: GO_Central

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-8853383 Lysosomal oligosaccharide catabolism

Protein family/group databases

CAZyiGH38 Glycoside Hydrolase Family 38

Names & Taxonomyi

Protein namesi
Recommended name:
Epididymis-specific alpha-mannosidase (EC:3.2.1.24)
Alternative name(s):
Mannosidase alpha class 2B member 2
Gene namesi
Name:Man2b2
Synonyms:Kiaa0935
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1195262 Man2b2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000001207822 – 1018Epididymis-specific alpha-mannosidaseAdd BLAST997

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi226N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi249N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi294N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi336N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi511N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi528N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi620N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi652N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi818N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi822N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiO54782
MaxQBiO54782
PaxDbiO54782
PeptideAtlasiO54782
PRIDEiO54782

PTM databases

iPTMnetiO54782
PhosphoSitePlusiO54782

Expressioni

Tissue specificityi

Mainly expressed in the narrow region between the caput and corpus epididymis.

Developmental stagei

Specifically expressed in type A spermatogonia at stages IX-XI of spermatogenesis. Detected there until the cell developed into type B spermatogonia.

Gene expression databases

BgeeiENSMUSG00000029119 Expressed in 205 organ(s), highest expression level in heart left ventricle
CleanExiMM_MAN2B2
ExpressionAtlasiO54782 baseline and differential
GenevisibleiO54782 MM

Interactioni

Protein-protein interaction databases

IntActiO54782, 1 interactor
MINTiO54782
STRINGi10090.ENSMUSP00000031002

Structurei

3D structure databases

ProteinModelPortaliO54782
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1959 Eukaryota
ENOG410XQMZ LUCA
GeneTreeiENSGT00510000046304
HOGENOMiHOG000113450
HOVERGENiHBG052392
InParanoidiO54782
KOiK12312
OMAiYWNGVAL
OrthoDBiEOG091G019Q
PhylomeDBiO54782
TreeFamiTF332447

Family and domain databases

Gene3Di1.20.1270.50, 1 hit
2.60.40.1180, 1 hit
3.20.110.10, 1 hit
InterProiView protein in InterPro
IPR011013 Gal_mutarotase_sf_dom
IPR011330 Glyco_hydro/deAcase_b/a-brl
IPR011682 Glyco_hydro_38_C
IPR015341 Glyco_hydro_38_cen
IPR037094 Glyco_hydro_38_cen_sf
IPR000602 Glyco_hydro_38_N
IPR027291 Glyco_hydro_38_N_sf
IPR028995 Glyco_hydro_57/38_cen_sf
IPR013780 Glyco_hydro_b
PfamiView protein in Pfam
PF09261 Alpha-mann_mid, 1 hit
PF01074 Glyco_hydro_38, 1 hit
PF07748 Glyco_hydro_38C, 1 hit
SMARTiView protein in SMART
SM00872 Alpha-mann_mid, 1 hit
SUPFAMiSSF74650 SSF74650, 1 hit
SSF88688 SSF88688, 1 hit
SSF88713 SSF88713, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

O54782-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGPLRWLPLL GQLLLLWPRA AQPAGPIRAF VVPHSHMDVG WVFTVQESMR
60 70 80 90 100
AYAANVYTTV VAELVRGGQR RFIAVEQEFF RLWWDGVASE QQKQQVRQLL
110 120 130 140 150
HEGRLEFVLG GQVMHDEAVT HLDDQILQLT EGHGFLYETF GIRPQFSWHV
160 170 180 190 200
DPFGASATTP TLFALAGFNA HLISRIDYDL KDAMQEAQML QFVWHGSPSL
210 220 230 240 250
SGQQEIFTHV MDHYSYCTPS HIPFSNRSGF YWNGVAVFPE PPPDGVYPNM
260 270 280 290 300
SEPVTGANIH LYAEALVANV KQRAAWFRTP HVLWPWGCDK QFFNASVQFD
310 320 330 340 350
NMDPLLDYIN QRTAQFGISV QYATLNDYFQ ALHATNMTWG IRDHQDFLPY
360 370 380 390 400
SSEPLQAWTG FYTSRSTLKG LARQASALLY AGESMFTRYM WPDPSGTLDP
410 420 430 440 450
TWALQQLQQL RWAVSEVQHH DAITGTESPK VKNMYTEHLR MGMLGVRKLM
460 470 480 490 500
VSIALGGPPG SGTGAPKDIM GPQVTPVLSV DTRPVGYSAS VYNPLAWKIT
510 520 530 540 550
TIITLTVAFP NVSVTDELGH PVSTQIQNST KDPSAYDLLI LTTIPGLNYR
560 570 580 590 600
HYQVMHARGD QAGTRELVAP RANTLKFSLK LRNQPSQEGK RLVPVMNDCY
610 620 630 640 650
ILLFDQDTNM LHSIQDRQSN RTVRMTQEFL EYQANWDVKQ GPISDNYLFA
660 670 680 690 700
PNNTAEPSWE AVGMEMVAGT LVTDIRQYFY RYITDQEYIY SIHTRLAHPS
710 720 730 740 750
LAGELLCQRI EQQYRVGPLD LNREAILRTS SDLNSQQVLY SDNNGYQMQR
760 770 780 790 800
RPYKAFKSNP IPRNYYPMVQ SAFIEDDKSR LVLLAERPHG VSSQGNGQVE
810 820 830 840 850
VMLHRRLWNN LAWDLKYNLT LNDTSIVHPV LWLMLGPKST MTALHPRSGV
860 870 880 890 900
ALQHGPVVLL KELADEETPV HGPHNPWPVT LPPNLHLQIL SVPGWTYSRS
910 920 930 940 950
HAQHLRNLQR GHPEKPQANL QRVLLRLRHL YEAGEDPVLS RPATVDLKVV
960 970 980 990 1000
LRGLGSVVAV EERSLTGTWD VQMLQRWHWS TKTDHLKGHP TSPPRPPGGS
1010
IITVYPKEIR TFFIKFQQ
Length:1,018
Mass (Da):115,610
Last modified:June 7, 2005 - v2
Checksum:i41AD7AAEA7CC88A9
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6TMZ3F6TMZ3_MOUSE
Epididymis-specific alpha-mannosida...
Man2b2
494Annotation score:
F6Z025F6Z025_MOUSE
Epididymis-specific alpha-mannosida...
Man2b2
104Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti201S → T in BAA24266 (PubMed:9425289).Curated1
Sequence conflicti337M → I in BAB23655 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006458 mRNA Translation: BAA24266.1
AB083124 Genomic DNA Translation: BAC53862.1
AK004900 mRNA Translation: BAB23655.1
AK040388 mRNA Translation: BAC30580.1
BC066211 mRNA Translation: AAH66211.1
AK173067 mRNA Translation: BAD32345.1
CCDSiCCDS19243.1
PIRiJC5799
RefSeqiNP_032576.2, NM_008550.2
UniGeneiMm.761

Genome annotation databases

EnsembliENSMUST00000031002; ENSMUSP00000031002; ENSMUSG00000029119
GeneIDi17160
KEGGimmu:17160
UCSCiuc008xfb.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006458 mRNA Translation: BAA24266.1
AB083124 Genomic DNA Translation: BAC53862.1
AK004900 mRNA Translation: BAB23655.1
AK040388 mRNA Translation: BAC30580.1
BC066211 mRNA Translation: AAH66211.1
AK173067 mRNA Translation: BAD32345.1
CCDSiCCDS19243.1
PIRiJC5799
RefSeqiNP_032576.2, NM_008550.2
UniGeneiMm.761

3D structure databases

ProteinModelPortaliO54782
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO54782, 1 interactor
MINTiO54782
STRINGi10090.ENSMUSP00000031002

Protein family/group databases

CAZyiGH38 Glycoside Hydrolase Family 38

PTM databases

iPTMnetiO54782
PhosphoSitePlusiO54782

Proteomic databases

EPDiO54782
MaxQBiO54782
PaxDbiO54782
PeptideAtlasiO54782
PRIDEiO54782

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031002; ENSMUSP00000031002; ENSMUSG00000029119
GeneIDi17160
KEGGimmu:17160
UCSCiuc008xfb.2 mouse

Organism-specific databases

CTDi23324
MGIiMGI:1195262 Man2b2
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1959 Eukaryota
ENOG410XQMZ LUCA
GeneTreeiENSGT00510000046304
HOGENOMiHOG000113450
HOVERGENiHBG052392
InParanoidiO54782
KOiK12312
OMAiYWNGVAL
OrthoDBiEOG091G019Q
PhylomeDBiO54782
TreeFamiTF332447

Enzyme and pathway databases

ReactomeiR-MMU-8853383 Lysosomal oligosaccharide catabolism

Miscellaneous databases

ChiTaRSiMan2b2 mouse
PROiPR:O54782
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029119 Expressed in 205 organ(s), highest expression level in heart left ventricle
CleanExiMM_MAN2B2
ExpressionAtlasiO54782 baseline and differential
GenevisibleiO54782 MM

Family and domain databases

Gene3Di1.20.1270.50, 1 hit
2.60.40.1180, 1 hit
3.20.110.10, 1 hit
InterProiView protein in InterPro
IPR011013 Gal_mutarotase_sf_dom
IPR011330 Glyco_hydro/deAcase_b/a-brl
IPR011682 Glyco_hydro_38_C
IPR015341 Glyco_hydro_38_cen
IPR037094 Glyco_hydro_38_cen_sf
IPR000602 Glyco_hydro_38_N
IPR027291 Glyco_hydro_38_N_sf
IPR028995 Glyco_hydro_57/38_cen_sf
IPR013780 Glyco_hydro_b
PfamiView protein in Pfam
PF09261 Alpha-mann_mid, 1 hit
PF01074 Glyco_hydro_38, 1 hit
PF07748 Glyco_hydro_38C, 1 hit
SMARTiView protein in SMART
SM00872 Alpha-mann_mid, 1 hit
SUPFAMiSSF74650 SSF74650, 1 hit
SSF88688 SSF88688, 1 hit
SSF88713 SSF88713, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMA2B2_MOUSE
AccessioniPrimary (citable) accession number: O54782
Secondary accession number(s): Q69ZV1, Q8BH85, Q9DBK2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: June 7, 2005
Last modified: November 7, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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