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Protein

SRSF protein kinase 2

Gene

Srpk2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up-regulation. Plays an essential role in spliceosomal B complex formation via the phosphorylation of DDX23/PRP28.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Mg2+1 Publication

Activity regulationi

Activated by phosphorylation on Ser-50 and Ser-581.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei108ATPPROSITE-ProRule annotationBy similarity1
Active sitei212Proton acceptorPROSITE-ProRule annotationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi85 – 93ATPPROSITE-ProRule annotationBy similarity9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processDifferentiation, mRNA processing, mRNA splicing
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
SRSF protein kinase 2 (EC:2.7.11.1)
Alternative name(s):
SFRS protein kinase 2
Serine/arginine-rich protein-specific kinase 2
Short name:
SR-protein-specific kinase 2
Cleaved into the following 2 chains:
Gene namesi
Name:Srpk2Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1201408 Srpk2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000866781 – 681SRSF protein kinase 2Add BLAST681
ChainiPRO_00004147531 – 137SRSF protein kinase 2 N-terminalAdd BLAST137
ChainiPRO_0000414754138 – 681SRSF protein kinase 2 C-terminalAdd BLAST544

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei50PhosphoserineCombined sources1
Modified residuei331PhosphothreonineCombined sources1
Modified residuei332PhosphothreonineCombined sources1
Modified residuei378PhosphoserineCombined sources1
Modified residuei468PhosphoserineCombined sources1
Modified residuei471PhosphothreonineCombined sources1
Modified residuei477PhosphoserineCombined sources1
Modified residuei479PhosphoserineCombined sources1
Modified residuei483PhosphoserineCombined sources1
Modified residuei485Phosphothreonine; by PKB/AKT1By similarity1
Modified residuei487PhosphoserineCombined sources1
Modified residuei490PhosphoserineBy similarity1
Modified residuei581Phosphoserine; by CK2By similarity1

Post-translational modificationi

Phosphorylation at Thr-485 by PKB/AKT1 enhances its stimulatory activity in triggering cyclin-D1 (CCND1) expression and promoting apoptosis in neurons, which can be blocked by YWHAB. It also enhances its protein kinase activity toward ACIN1 and SRSF2, promotes its nuclear translocation and prevents its proteolytic cleavage (By similarity).By similarity
Proteolytically cleaved at Asp-137 and Asp-401 by caspase-3 during apoptotic cell death. Cleavage at Asp-137 which is the major site of cleavage, produces a small N-terminal fragment that translocates into nucleus and promotes VP16-induced apoptosis (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei137 – 138Cleavage; by caspase-3By similarity2
Sitei401 – 402Cleavage; by caspase-3By similarity2

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO54781
MaxQBiO54781
PaxDbiO54781
PeptideAtlasiO54781
PRIDEiO54781

PTM databases

iPTMnetiO54781
PhosphoSitePlusiO54781

Expressioni

Tissue specificityi

Expressed in testes, lung and brain.1 Publication

Gene expression databases

CleanExiMM_SRPK2

Interactioni

Subunit structurei

Interacts with PKB/AKT1 in a phosphorylation-dependent manner. The phosphorylated form (by PKB/AKT1) interacts with YWHAB and YWHAE. Interaction with YWHAB suppresses its cleavage by caspases and inhibits the release of its N-terminal pro-apoptotic fragment. Interacts with SFN. Associates with U4/U6-U5 tri-small nuclear ribonucleoproteins (U4/U6-U5 tri-snRNPs) (By similarity). Interacts with ACIN1.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SRSF1Q079553EBI-593325,EBI-398920From Homo sapiens.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203503, 3 interactors
IntActiO54781, 4 interactors
STRINGi10090.ENSMUSP00000085734

Structurei

3D structure databases

ProteinModelPortaliO54781
SMRiO54781
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini79 – 681Protein kinasePROSITE-ProRule annotationAdd BLAST603

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1290 Eukaryota
ENOG410XRBH LUCA
HOGENOMiHOG000171558
HOVERGENiHBG108512
InParanoidiO54781
KOiK08831
PhylomeDBiO54781
TreeFamiTF105334

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 2 hits
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All

O54781-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSVNSEKSSS SERPEPQQKA PLVPPPPPPP PPPPLPDPAP PEPEEEILGS
60 70 80 90 100
DDEEQEDPAD YCKGGYHPVK IGDLFNGRYH VIRKLGWGHF STVWLCWDMQ
110 120 130 140 150
GKRFVAMKVV KSAQHYTETA LDEIKLLKCV RESDPSDPNK DMVVQLIDDF
160 170 180 190 200
KISGMNGIHV CMVFEVLGHH LLKWIIKSNY QGLPVRCVKS IIRQVLQGLD
210 220 230 240 250
YLHSKCKIIH TDIKPENILM CVDDAYVRRM AAEATEWQKA GAPPPSGSAV
260 270 280 290 300
STAPQQKPIG KISKNKKKKL KKKQKRQAEL LEKRLQEIEE LEREAERKIL
310 320 330 340 350
EENITSAEAS GEQDGEYQPE VTLKAADLED TTEEETAKDN GEVEDQEEKE
360 370 380 390 400
DAEKENAEKD EDDVEQELAN LDPTWVESPK ANGHIENGPF SLEQQLEDEE
410 420 430 440 450
DDEDDCANPE EYNLDEPNAE SDYTYSSSYE QFNGELPNGQ HKTSEFPTPL
460 470 480 490 500
FSGPLEPVAC GSVISEGSPL TEQEESSPSH DRSRTVSASS TGDLPKTKTR
510 520 530 540 550
AADLLVNPLD PRNADKIRVK IADLGNACWV HKHFTEDIQT RQYRSIEVLI
560 570 580 590 600
GAGYSTPADI WSTACMAFEL ATGDYLFEPH SGEDYSRDED HIAHIIELLG
610 620 630 640 650
SIPRHFALSG KYSREFFNRR GELRHITKLK PWSLFDVLVE KYGWPHEDAA
660 670 680
QFTDFLIPML EMVPEKRASA GECLRHPWLN S
Length:681
Mass (Da):76,757
Last modified:July 5, 2005 - v2
Checksum:i1857EA955B2F9C0E
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0R4J124A0A0R4J124_MOUSE
SRSF protein kinase 2
Srpk2 mCG_4527
682Annotation score:
Q6P5E1Q6P5E1_MOUSE
SRSF protein kinase 2
Srpk2
156Annotation score:
A0A0G2JG74A0A0G2JG74_MOUSE
SRSF protein kinase 2
Srpk2
134Annotation score:
A0A0G2JDY2A0A0G2JDY2_MOUSE
SRSF protein kinase 2
Srpk2
129Annotation score:
A0A0G2JDT6A0A0G2JDT6_MOUSE
SRSF protein kinase 2
Srpk2
57Annotation score:
A0A0G2JEI1A0A0G2JEI1_MOUSE
SRSF protein kinase 2
Srpk2
22Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti313Q → QQ in AAH20178 (PubMed:15489334).Curated1
Sequence conflicti368L → P in BAA24055 (PubMed:9446799).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006036 mRNA Translation: BAA24055.1
BC020178 mRNA Translation: AAH20178.1
PIRiJC5929
RefSeqiNP_033300.2, NM_009274.2
XP_017176258.1, XM_017320769.1
UniGeneiMm.288728

Genome annotation databases

GeneIDi20817
KEGGimmu:20817
UCSCiuc008wqe.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006036 mRNA Translation: BAA24055.1
BC020178 mRNA Translation: AAH20178.1
PIRiJC5929
RefSeqiNP_033300.2, NM_009274.2
XP_017176258.1, XM_017320769.1
UniGeneiMm.288728

3D structure databases

ProteinModelPortaliO54781
SMRiO54781
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203503, 3 interactors
IntActiO54781, 4 interactors
STRINGi10090.ENSMUSP00000085734

PTM databases

iPTMnetiO54781
PhosphoSitePlusiO54781

Proteomic databases

EPDiO54781
MaxQBiO54781
PaxDbiO54781
PeptideAtlasiO54781
PRIDEiO54781

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi20817
KEGGimmu:20817
UCSCiuc008wqe.1 mouse

Organism-specific databases

CTDi6733
MGIiMGI:1201408 Srpk2

Phylogenomic databases

eggNOGiKOG1290 Eukaryota
ENOG410XRBH LUCA
HOGENOMiHOG000171558
HOVERGENiHBG108512
InParanoidiO54781
KOiK08831
PhylomeDBiO54781
TreeFamiTF105334

Miscellaneous databases

ChiTaRSiSrpk2 mouse
PROiPR:O54781
SOURCEiSearch...

Gene expression databases

CleanExiMM_SRPK2

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 2 hits
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSRPK2_MOUSE
AccessioniPrimary (citable) accession number: O54781
Secondary accession number(s): Q8VCD9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2005
Last modified: November 7, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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Main funding by: National Institutes of Health

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