UniProtKB - O54781 (SRPK2_MOUSE)
SRSF protein kinase 2
Srpk2
Functioni
Catalytic activityi
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 108 | ATPPROSITE-ProRule annotationBy similarity | 1 | |
Active sitei | 212 | Proton acceptorPROSITE-ProRule annotationBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 85 – 93 | ATPPROSITE-ProRule annotationBy similarity | 9 |
GO - Molecular functioni
- 14-3-3 protein binding Source: BHF-UCL
- ATP binding Source: UniProtKB
- magnesium ion binding Source: UniProtKB
- MAP kinase activity Source: GO_Central
- protein serine/threonine kinase activity Source: UniProtKB
GO - Biological processi
- angiogenesis Source: BHF-UCL
- cell differentiation Source: UniProtKB-KW
- intracellular signal transduction Source: UniProtKB
- negative regulation of viral genome replication Source: BHF-UCL
- nuclear speck organization Source: BHF-UCL
- peptidyl-serine phosphorylation Source: MGI
- positive regulation of cell cycle Source: BHF-UCL
- positive regulation of cell population proliferation Source: BHF-UCL
- positive regulation of gene expression Source: BHF-UCL
- positive regulation of neuron apoptotic process Source: BHF-UCL
- positive regulation of viral genome replication Source: BHF-UCL
- protein phosphorylation Source: UniProtKB
- regulation of gene expression Source: GO_Central
- regulation of mRNA processing Source: GO_Central
- R-loop disassembly Source: UniProtKB
- RNA splicing Source: UniProtKB
- spliceosomal complex assembly Source: UniProtKB
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Differentiation, mRNA processing, mRNA splicing |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: SRSF protein kinase 2 (EC:2.7.11.11 Publication)Alternative name(s): SFRS protein kinase 2 Serine/arginine-rich protein-specific kinase 2 Short name: SR-protein-specific kinase 2 Cleaved into the following 2 chains: |
Gene namesi | Name:Srpk2Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1201408, Srpk2 |
Subcellular locationi
Nucleus
- nucleoplasm 1 Publication
- Nucleus speckle By similarity
Other locations
- Cytoplasm 1 Publication
- Chromosome By similarity
Note: Shuttles between the nucleus and the cytoplasm (By similarity). KAT5/TIP60 inhibits its nuclear translocation (By similarity). Phosphorylation at Thr-492 by PKB/AKT1 promotes nuclear translocation (By similarity). Preferentially localizes across the entire gene coding region (By similarity). During transcription, accumulates at chromatin loci where unscheduled R-loops form and colocalizes with paused 'Ser-5'-phosphorlyated POLR2A/RNA polymerase II and helicase DDX23 (By similarity).By similarity
Cytosol
- cytosol Source: MGI
Nucleus
- nuclear speck Source: UniProtKB
- nucleolus Source: MGI
- nucleoplasm Source: UniProtKB
- nucleus Source: UniProtKB
Other locations
Keywords - Cellular componenti
Chromosome, Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086678 | 1 – 681 | SRSF protein kinase 2Add BLAST | 681 | |
ChainiPRO_0000414753 | 1 – 137 | SRSF protein kinase 2 N-terminalAdd BLAST | 137 | |
ChainiPRO_0000414754 | 138 – 681 | SRSF protein kinase 2 C-terminalAdd BLAST | 544 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 50 | PhosphoserineCombined sources | 1 | |
Modified residuei | 331 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 332 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 378 | PhosphoserineCombined sources | 1 | |
Modified residuei | 468 | PhosphoserineCombined sources | 1 | |
Modified residuei | 471 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 477 | PhosphoserineCombined sources | 1 | |
Modified residuei | 479 | PhosphoserineCombined sources | 1 | |
Modified residuei | 483 | PhosphoserineCombined sources | 1 | |
Modified residuei | 485 | Phosphothreonine; by PKB/AKT1By similarity | 1 | |
Modified residuei | 487 | PhosphoserineCombined sources | 1 | |
Modified residuei | 490 | PhosphoserineBy similarity | 1 | |
Modified residuei | 581 | Phosphoserine; by CK2By similarity | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 137 – 138 | Cleavage; by caspase-3By similarity | 2 | |
Sitei | 401 – 402 | Cleavage; by caspase-3By similarity | 2 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | O54781 |
MaxQBi | O54781 |
PaxDbi | O54781 |
PeptideAtlasi | O54781 |
PRIDEi | O54781 |
PTM databases
iPTMneti | O54781 |
PhosphoSitePlusi | O54781 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Associates with U4/U6-U5 tri-small nuclear ribonucleoproteins (U4/U6-U5 tri-snRNPs) (By similarity).
Interacts with PKB/AKT1 in a phosphorylation-dependent manner (By similarity). The phosphorylated form (by PKB/AKT1) interacts with YWHAB and YWHAE (By similarity). Interaction with YWHAB suppresses its cleavage by caspases and inhibits the release of its N-terminal pro-apoptotic fragment (By similarity).
Interacts with SFN (By similarity).
Interacts with ACIN1 (PubMed:18559500).
Interacts with POLR2A/RNA polymerase II; the interaction occurs during the co-transcriptional formation of inappropriate R-loops (By similarity).
By similarity1 PublicationBinary interactionsi
O54781
With | #Exp. | IntAct |
---|---|---|
SRSF1 [Q07955] from Homo sapiens. | 3 | EBI-593325,EBI-398920 |
GO - Molecular functioni
- 14-3-3 protein binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 203503, 23 interactors |
IntActi | O54781, 4 interactors |
STRINGi | 10090.ENSMUSP00000085734 |
Miscellaneous databases
RNActi | O54781, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 79 – 681 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 603 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1290, Eukaryota |
InParanoidi | O54781 |
OrthoDBi | 290680at2759 |
PhylomeDBi | O54781 |
TreeFami | TF105334 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 2 hits |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MSVNSEKSSS SERPEPQQKA PLVPPPPPPP PPPPLPDPAP PEPEEEILGS
60 70 80 90 100
DDEEQEDPAD YCKGGYHPVK IGDLFNGRYH VIRKLGWGHF STVWLCWDMQ
110 120 130 140 150
GKRFVAMKVV KSAQHYTETA LDEIKLLKCV RESDPSDPNK DMVVQLIDDF
160 170 180 190 200
KISGMNGIHV CMVFEVLGHH LLKWIIKSNY QGLPVRCVKS IIRQVLQGLD
210 220 230 240 250
YLHSKCKIIH TDIKPENILM CVDDAYVRRM AAEATEWQKA GAPPPSGSAV
260 270 280 290 300
STAPQQKPIG KISKNKKKKL KKKQKRQAEL LEKRLQEIEE LEREAERKIL
310 320 330 340 350
EENITSAEAS GEQDGEYQPE VTLKAADLED TTEEETAKDN GEVEDQEEKE
360 370 380 390 400
DAEKENAEKD EDDVEQELAN LDPTWVESPK ANGHIENGPF SLEQQLEDEE
410 420 430 440 450
DDEDDCANPE EYNLDEPNAE SDYTYSSSYE QFNGELPNGQ HKTSEFPTPL
460 470 480 490 500
FSGPLEPVAC GSVISEGSPL TEQEESSPSH DRSRTVSASS TGDLPKTKTR
510 520 530 540 550
AADLLVNPLD PRNADKIRVK IADLGNACWV HKHFTEDIQT RQYRSIEVLI
560 570 580 590 600
GAGYSTPADI WSTACMAFEL ATGDYLFEPH SGEDYSRDED HIAHIIELLG
610 620 630 640 650
SIPRHFALSG KYSREFFNRR GELRHITKLK PWSLFDVLVE KYGWPHEDAA
660 670 680
QFTDFLIPML EMVPEKRASA GECLRHPWLN S
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0R4J124 | A0A0R4J124_MOUSE | SRSF protein kinase 2 | Srpk2 | 682 | Annotation score: | ||
A0A0G2JDY2 | A0A0G2JDY2_MOUSE | SRSF protein kinase 2 | Srpk2 | 129 | Annotation score: | ||
A0A0G2JDT6 | A0A0G2JDT6_MOUSE | SRSF protein kinase 2 | Srpk2 | 57 | Annotation score: | ||
A0A0G2JEI1 | A0A0G2JEI1_MOUSE | SRSF protein kinase 2 | Srpk2 | 22 | Annotation score: | ||
A0A0G2JG74 | A0A0G2JG74_MOUSE | SRSF protein kinase 2 | Srpk2 | 134 | Annotation score: | ||
Q6P5E1 | Q6P5E1_MOUSE | SRSF protein kinase 2 | Srpk2 | 156 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 313 | Q → QQ in AAH20178 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 368 | L → P in BAA24055 (PubMed:9446799).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB006036 mRNA Translation: BAA24055.1 BC020178 mRNA Translation: AAH20178.1 |
PIRi | JC5929 |
RefSeqi | NP_033300.2, NM_009274.2 XP_017176258.1, XM_017320769.1 |
Genome annotation databases
GeneIDi | 20817 |
KEGGi | mmu:20817 |
UCSCi | uc008wqe.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB006036 mRNA Translation: BAA24055.1 BC020178 mRNA Translation: AAH20178.1 |
PIRi | JC5929 |
RefSeqi | NP_033300.2, NM_009274.2 XP_017176258.1, XM_017320769.1 |
3D structure databases
SMRi | O54781 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 203503, 23 interactors |
IntActi | O54781, 4 interactors |
STRINGi | 10090.ENSMUSP00000085734 |
PTM databases
iPTMneti | O54781 |
PhosphoSitePlusi | O54781 |
Proteomic databases
EPDi | O54781 |
MaxQBi | O54781 |
PaxDbi | O54781 |
PeptideAtlasi | O54781 |
PRIDEi | O54781 |
Genome annotation databases
GeneIDi | 20817 |
KEGGi | mmu:20817 |
UCSCi | uc008wqe.1, mouse |
Organism-specific databases
CTDi | 6733 |
MGIi | MGI:1201408, Srpk2 |
Phylogenomic databases
eggNOGi | KOG1290, Eukaryota |
InParanoidi | O54781 |
OrthoDBi | 290680at2759 |
PhylomeDBi | O54781 |
TreeFami | TF105334 |
Miscellaneous databases
BioGRID-ORCSi | 20817, 5 hits in 18 CRISPR screens |
ChiTaRSi | Srpk2, mouse |
PROi | PR:O54781 |
RNActi | O54781, protein |
SOURCEi | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 2 hits |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SRPK2_MOUSE | |
Accessioni | O54781Primary (citable) accession number: O54781 Secondary accession number(s): Q8VCD9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 5, 2005 |
Last sequence update: | July 5, 2005 | |
Last modified: | December 2, 2020 | |
This is version 152 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human and mouse protein kinases
Human and mouse protein kinases: classification and index