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Protein

Zona pellucida sperm-binding protein 1

Gene

Zp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP1 ensures the structural integrity of the zona pellucida.

GO - Biological processi

Keywordsi

Biological processFertilization

Names & Taxonomyi

Protein namesi
Recommended name:
Zona pellucida sperm-binding protein 1
Alternative name(s):
Zona pellucida glycoprotein 1
Short name:
Zp-1
Cleaved into the following chain:
Gene namesi
Name:Zp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620604 Zp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 588ExtracellularSequence analysisAdd BLAST568
Transmembranei589 – 609HelicalSequence analysisAdd BLAST21
Topological domaini610 – 617CytoplasmicSequence analysis8

Keywords - Cellular componenti

Cell membrane, Extracellular matrix, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 201 PublicationAdd BLAST20
ChainiPRO_000004168121 – 544Zona pellucida sperm-binding protein 1Add BLAST524
ChainiPRO_000030455521 – ?Processed zona pellucida sperm-binding protein 1
PropeptideiPRO_0000041682545 – 617Removed in mature form1 PublicationAdd BLAST73

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei21Pyrrolidone carboxylic acid1 Publication1
Glycosylationi49N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi68N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi226 ↔ 251PROSITE-ProRule annotation
Disulfide bondi235 ↔ 250PROSITE-ProRule annotation
Disulfide bondi245 ↔ 260PROSITE-ProRule annotation
Glycosylationi369N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi447 ↔ 468PROSITE-ProRule annotation1 Publication

Post-translational modificationi

Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
O-glycosylated.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiO54766
PRIDEiO54766

PTM databases

iPTMnetiO54766

Expressioni

Tissue specificityi

Oocytes.

Interactioni

Subunit structurei

Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers. Interacts with ZP3.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028396

Structurei

3D structure databases

ProteinModelPortaliO54766
SMRiO54766
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini224 – 264P-typePROSITE-ProRule annotationAdd BLAST41
Domaini269 – 540ZPPROSITE-ProRule annotationAdd BLAST272

Domaini

The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.

Sequence similaritiesi

Belongs to the ZP domain family. ZPB subfamily.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IE4J Eukaryota
ENOG4111H75 LUCA
HOGENOMiHOG000118071
HOVERGENiHBG036289
InParanoidiO54766
KOiK19926
PhylomeDBiO54766

Family and domain databases

CDDicd00111 Trefoil, 1 hit
InterProiView protein in InterPro
IPR017957 P_trefoil_CS
IPR000519 P_trefoil_dom
IPR001507 ZP_dom
IPR017977 ZP_dom_CS
PfamiView protein in Pfam
PF00088 Trefoil, 1 hit
PF00100 Zona_pellucida, 1 hit
PRINTSiPR00023 ZPELLUCIDA
SMARTiView protein in SMART
SM00018 PD, 1 hit
SM00241 ZP, 1 hit
PROSITEiView protein in PROSITE
PS00025 P_TREFOIL_1, 1 hit
PS51448 P_TREFOIL_2, 1 hit
PS00682 ZP_1, 1 hit
PS51034 ZP_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O54766-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAWGCFVVLL LLVAAPLRLG QHLHLKPGFQ YSYDCGVQGM QLLVFPRPNQ
60 70 80 90 100
TIQFKVLDEF GNRFEVNNCS ICYHWVISEA QKPAVFSADY KGCHVLEKQD
110 120 130 140 150
GRFHLRVFIQ AVLPNGRVDT AQDVTLICPK PDHILTPESY LAPPTTPQPF
160 170 180 190 200
IPHTFALHPI SGHTLAGSGH TGLTTLYPET HPTPAPPSSE PGPVGPTVPQ
210 220 230 240 250
SQWGTLGSWE LTELDSIGTH LLQERCQVAS GHIPCMVKGS SEEACQQAGC
260 270 280 290 300
CYDNTKEMPC YYGNTVTLQC FRSGYFTLVM SQETALTHGV MLDNVHLAYA
310 320 330 340 350
PNGCPPTQKT SAFVVFHVPL TLCGTAIQVV GKQLVYENQL VSNIEVQTGP
360 370 380 390 400
QGSITRDGVF RLHVRCIFNA SDFLPIRASI FSPQPPAPVT RSGPLRLELR
410 420 430 440 450
IATDKTFSSY YQGSDYPLVR LLQEPVYIEV RLLQRTDPGL ALMLHQCWAT
460 470 480 490 500
PSASPFEQPQ WPILSDGCPF KGDNYRTQMV AADRATLPFW SHYQRFTIAT
510 520 530 540 550
FTLLDSSSQN ALRGQVYFFC SASACHPVGS ETCSTTCDSE IARHRRSSGH
560 570 580 590 600
HNSTIRALDI VSSPGAVGFE DAPKLEPSGS TRNSGSRPLL WVLQLLALTL
610
VLGDGVLVGL SWAWAWA
Length:617
Mass (Da):67,853
Last modified:June 1, 1998 - v1
Checksum:iAF52D9227F8A4CCC
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3V8U5G3V8U5_RAT
RCG47565
Zp1 rCG_47565
617Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000928 mRNA Translation: BAA24486.1
RefSeqiNP_445961.1, NM_053509.1
UniGeneiRn.10890

Genome annotation databases

GeneIDi85271
KEGGirno:85271
UCSCiRGD:620604 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000928 mRNA Translation: BAA24486.1
RefSeqiNP_445961.1, NM_053509.1
UniGeneiRn.10890

3D structure databases

ProteinModelPortaliO54766
SMRiO54766
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028396

PTM databases

iPTMnetiO54766

Proteomic databases

PaxDbiO54766
PRIDEiO54766

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi85271
KEGGirno:85271
UCSCiRGD:620604 rat

Organism-specific databases

CTDi22917
RGDi620604 Zp1

Phylogenomic databases

eggNOGiENOG410IE4J Eukaryota
ENOG4111H75 LUCA
HOGENOMiHOG000118071
HOVERGENiHBG036289
InParanoidiO54766
KOiK19926
PhylomeDBiO54766

Miscellaneous databases

PROiPR:O54766

Family and domain databases

CDDicd00111 Trefoil, 1 hit
InterProiView protein in InterPro
IPR017957 P_trefoil_CS
IPR000519 P_trefoil_dom
IPR001507 ZP_dom
IPR017977 ZP_dom_CS
PfamiView protein in Pfam
PF00088 Trefoil, 1 hit
PF00100 Zona_pellucida, 1 hit
PRINTSiPR00023 ZPELLUCIDA
SMARTiView protein in SMART
SM00018 PD, 1 hit
SM00241 ZP, 1 hit
PROSITEiView protein in PROSITE
PS00025 P_TREFOIL_1, 1 hit
PS51448 P_TREFOIL_2, 1 hit
PS00682 ZP_1, 1 hit
PS51034 ZP_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiZP1_RAT
AccessioniPrimary (citable) accession number: O54766
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: June 1, 1998
Last modified: June 20, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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