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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit

Gene

Ddost

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Required for the assembly of both SST3A- and SS3B-containing OST complexes. Required for efficient N-glycosylation.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation
UniPathwayi
UPA00378

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
Short name:
DDOST 48 kDa subunit
Short name:
Oligosaccharyl transferase 48 kDa subunit
Gene namesi
Name:Ddost
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1194508 Ddost

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini29 – 412LumenalSequence analysisAdd BLAST384
Transmembranei413 – 432HelicalSequence analysisAdd BLAST20
Topological domaini433 – 441CytoplasmicSequence analysis9

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28By similarityAdd BLAST28
ChainiPRO_000002195829 – 441Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunitAdd BLAST413

Proteomic databases

EPDiO54734
MaxQBiO54734
PaxDbiO54734
PeptideAtlasiO54734
PRIDEiO54734
TopDownProteomicsiO54734

PTM databases

iPTMnetiO54734
PhosphoSitePlusiO54734
SwissPalmiO54734

Expressioni

Gene expression databases

BgeeiENSMUSG00000028757 Expressed in 303 organ(s), highest expression level in islet of Langerhans
GenevisibleiO54734 MM

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity).By similarity

Protein-protein interaction databases

BioGridi199080, 3 interactors
IntActiO54734, 8 interactors
MINTiO54734
STRINGi10090.ENSMUSP00000030538

Structurei

3D structure databases

ProteinModelPortaliO54734
SMRiO54734
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DDOST 48 kDa subunit family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2754 Eukaryota
ENOG410XSF3 LUCA
GeneTreeiENSGT00390000017294
HOGENOMiHOG000173744
HOVERGENiHBG053378
InParanoidiO54734
KOiK12670
OMAiAFSMMIG
OrthoDBiEOG091G07Z5
TreeFamiTF314821

Family and domain databases

InterProiView protein in InterPro
IPR005013 DDOST_48_kDa_subunit
PANTHERiPTHR10830 PTHR10830, 1 hit
PfamiView protein in Pfam
PF03345 DDOST_48kD, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O54734-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKMDPRLAVR AWPLCGLLLA VLGCVCASGP RTLVLLDNLN VRDTHSLFFR
60 70 80 90 100
SLKDRGFELT FKTADDPSLS LIKYGEFLYD NLIIFSPSVE DFGGNINVET
110 120 130 140 150
ISAFIDGGGS VLVAASSDIG DPLRELGSEC GIEFDEEKTA VIDHHNYDVS
160 170 180 190 200
DLGQHTLIVA DTENLLKAPT IVGKSSLNPI LFRGVGMVAD PDNPLVLDIL
210 220 230 240 250
TGSSTSYSFF PDKPITQYPH AVGRNTLLIA GLQARNNARV IFSGSLDFFS
260 270 280 290 300
DAFFNSAVQK ATPGAQRYSQ TGNYELAVAL SRWVFKEEGV LRVGPVSHHR
310 320 330 340 350
VGEMAPPNAY TVTDLVEYSI IIEQLSNGKW VPFDGDDIQL EFVRIDPFVR
360 370 380 390 400
TFLKRKGGKY SVQFKLPDVY GVFQFKVDYN RLGYTHLYSS TQVSVRPLQH
410 420 430 440
TQYERFIPSA YPYYASAFSM MAGLFIFSIV FLHMKEKEKS D
Length:441
Mass (Da):49,028
Last modified:July 27, 2011 - v2
Checksum:iC1B9A0B94D2A1CAC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti321I → V in BAA23671 (PubMed:9367678).Curated1
Sequence conflicti321I → V in BAB82434 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89063 mRNA Translation: BAA23671.1
AB012717 Genomic DNA Translation: BAB82434.1
AK081542 mRNA Translation: BAC38250.1
AL807249 Genomic DNA Translation: CAM18871.1
BC068132 mRNA Translation: AAH68132.1
CCDSiCCDS18824.1
RefSeqiNP_031864.2, NM_007838.2
UniGeneiMm.470295
Mm.7236

Genome annotation databases

EnsembliENSMUST00000030538; ENSMUSP00000030538; ENSMUSG00000028757
GeneIDi13200
KEGGimmu:13200
UCSCiuc008vkt.1 mouse

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89063 mRNA Translation: BAA23671.1
AB012717 Genomic DNA Translation: BAB82434.1
AK081542 mRNA Translation: BAC38250.1
AL807249 Genomic DNA Translation: CAM18871.1
BC068132 mRNA Translation: AAH68132.1
CCDSiCCDS18824.1
RefSeqiNP_031864.2, NM_007838.2
UniGeneiMm.470295
Mm.7236

3D structure databases

ProteinModelPortaliO54734
SMRiO54734
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199080, 3 interactors
IntActiO54734, 8 interactors
MINTiO54734
STRINGi10090.ENSMUSP00000030538

PTM databases

iPTMnetiO54734
PhosphoSitePlusiO54734
SwissPalmiO54734

Proteomic databases

EPDiO54734
MaxQBiO54734
PaxDbiO54734
PeptideAtlasiO54734
PRIDEiO54734
TopDownProteomicsiO54734

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030538; ENSMUSP00000030538; ENSMUSG00000028757
GeneIDi13200
KEGGimmu:13200
UCSCiuc008vkt.1 mouse

Organism-specific databases

CTDi1650
MGIiMGI:1194508 Ddost

Phylogenomic databases

eggNOGiKOG2754 Eukaryota
ENOG410XSF3 LUCA
GeneTreeiENSGT00390000017294
HOGENOMiHOG000173744
HOVERGENiHBG053378
InParanoidiO54734
KOiK12670
OMAiAFSMMIG
OrthoDBiEOG091G07Z5
TreeFamiTF314821

Enzyme and pathway databases

UniPathwayi
UPA00378

ReactomeiR-MMU-6798695 Neutrophil degranulation

Miscellaneous databases

ChiTaRSiDdost mouse
PROiPR:O54734
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028757 Expressed in 303 organ(s), highest expression level in islet of Langerhans
GenevisibleiO54734 MM

Family and domain databases

InterProiView protein in InterPro
IPR005013 DDOST_48_kDa_subunit
PANTHERiPTHR10830 PTHR10830, 1 hit
PfamiView protein in Pfam
PF03345 DDOST_48kD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiOST48_MOUSE
AccessioniPrimary (citable) accession number: O54734
Secondary accession number(s): Q8C4P7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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