Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Matrix metalloproteinase-15

Gene

Mmp15

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A.

Cofactori

Protein has several cofactor binding sites:
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
  • Ca2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi107Zinc; in inhibited formBy similarity1
Metal bindingi255Zinc; catalyticPROSITE-ProRule annotation1
Active sitei256PROSITE-ProRule annotation1
Metal bindingi259Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi265Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • metalloendopeptidase activity Source: MGI
  • zinc ion binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandCalcium, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.24.B5 3474
ReactomeiR-MMU-1442490 Collagen degradation
R-MMU-1474228 Degradation of the extracellular matrix
R-MMU-1592389 Activation of Matrix Metalloproteinases

Protein family/group databases

MEROPSiM10.015

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix metalloproteinase-15 (EC:3.4.24.-)
Short name:
MMP-15
Alternative name(s):
Membrane-type matrix metalloproteinase 2
Short name:
MT-MMP 2
Short name:
MTMMP2
Membrane-type-2 matrix metalloproteinase
Short name:
MT2-MMP
Short name:
MT2MMP
Gene namesi
Name:Mmp15
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:109320 Mmp15

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini128 – 614ExtracellularSequence analysisAdd BLAST487
Transmembranei615 – 635HelicalSequence analysisAdd BLAST21
Topological domaini636 – 657CytoplasmicSequence analysisAdd BLAST22

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 36Sequence analysisAdd BLAST36
PropeptideiPRO_000002881037 – 127By similarityAdd BLAST91
ChainiPRO_0000028811128 – 657Matrix metalloproteinase-15Add BLAST530

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi146N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi366 ↔ 555By similarity
Glycosylationi414N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

The precursor is cleaved by a furin endopeptidase.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiO54732
PaxDbiO54732
PeptideAtlasiO54732
PRIDEiO54732

PTM databases

iPTMnetiO54732
PhosphoSitePlusiO54732
SwissPalmiO54732

Expressioni

Gene expression databases

BgeeiENSMUSG00000031790 Expressed in 286 organ(s), highest expression level in decidua
CleanExiMM_MMP15
GenevisibleiO54732 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034243

Structurei

3D structure databases

ProteinModelPortaliO54732
SMRiO54732
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati363 – 411Hemopexin 1Add BLAST49
Repeati412 – 457Hemopexin 2Add BLAST46
Repeati459 – 507Hemopexin 3Add BLAST49
Repeati508 – 555Hemopexin 4Add BLAST48

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi105 – 112Cysteine switchBy similarity8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi122 – 127Poly-Arg6

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Belongs to the peptidase M10A family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1565 Eukaryota
ENOG410XQ5D LUCA
GeneTreeiENSGT00760000118870
HOGENOMiHOG000217928
HOVERGENiHBG052484
InParanoidiO54732
KOiK07995
OMAiQHGDPGY
OrthoDBiEOG091G03DP
PhylomeDBiO54732
TreeFamiTF352396

Family and domain databases

CDDicd00094 HX, 1 hit
cd04278 ZnMc_MMP, 1 hit
Gene3Di2.110.10.10, 1 hit
3.40.390.10, 1 hit
InterProiView protein in InterPro
IPR000585 Hemopexin-like_dom
IPR036375 Hemopexin-like_dom_sf
IPR018487 Hemopexin-like_repeat
IPR018486 Hemopexin_CS
IPR033739 M10A_MMP
IPR024079 MetalloPept_cat_dom_sf
IPR028729 MMP15
IPR001818 Pept_M10_metallopeptidase
IPR021190 Pept_M10A
IPR021805 Pept_M10A_metallopeptidase_C
IPR021158 Pept_M10A_Zn_BS
IPR006026 Peptidase_Metallo
IPR002477 Peptidoglycan-bd-like
IPR036365 PGBD-like_sf
PANTHERiPTHR10201:SF25 PTHR10201:SF25, 1 hit
PfamiView protein in Pfam
PF11857 DUF3377, 1 hit
PF00045 Hemopexin, 4 hits
PF00413 Peptidase_M10, 1 hit
PF01471 PG_binding_1, 1 hit
PIRSFiPIRSF001191 Peptidase_M10A_matrix, 1 hit
PRINTSiPR00138 MATRIXIN
SMARTiView protein in SMART
SM00120 HX, 4 hits
SM00235 ZnMc, 1 hit
SUPFAMiSSF47090 SSF47090, 1 hit
SSF50923 SSF50923, 1 hit
PROSITEiView protein in PROSITE
PS00546 CYSTEINE_SWITCH, 1 hit
PS00024 HEMOPEXIN, 1 hit
PS51642 HEMOPEXIN_2, 4 hits
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O54732-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGSDRSALGR PGCTGSCLSS RASLLPLLLV LLDCLGHGTA SKDAEVYAAE
60 70 80 90 100
NWLRLYGYLP QPSRHMSTMR SAQILASALA EMQSFYGIPV TGVLDEETKT
110 120 130 140 150
WMKRPRCGVP DQFGVHVKAN LRRRRKRYTL TGKAWNNYHL TFSIQNYTEK
160 170 180 190 200
LGWYNSMEAV RRAFQVWEQV TPLVFQEVSY DDIRLRRRAE ADIMVLFASG
210 220 230 240 250
FHGDSSPFDG VGGFLAHAYF PGPGLGGDTH FDADEPWTFS STDLHGISLF
260 270 280 290 300
LVAVHELGHA LGLEHSSNPS AIMAPFYQWM DTDNFQLPED DLRGIQQLYG
310 320 330 340 350
SPDGKPQPTR PLPTVRPRRP GRPDHQPPRP PQPPHPGGKP ERPPKPGPPP
360 370 380 390 400
QPRATERPDQ YGPNICDGNF DTVAVLRGEM FVFKGRWFWR VRHNRVLDNY
410 420 430 440 450
PMPIGHFWRG LPGNISAAYE RQDGHFVFFK GNRYWLFREA NLEPGYPQPL
460 470 480 490 500
SSYGTDIPYD RIDTAIWWEP TGHTFFFQAD RYWRFNEETQ HGDPGYPKPI
510 520 530 540 550
SVWQGIPTSP KGAFLSNDAA YTYFYKGTKY WKFNNERLRM EPGHPKSILR
560 570 580 590 600
DFMGCQEHVE PRSRWPDVAR PPFNPNGGAE PEADGDSKEE NAGDKDEGSR
610 620 630 640 650
VVVQMEEVVR TVNVVMVLVP LLLLLCILGL AFALVQMQRK GAPRMLLYCK

RSLQEWV
Length:657
Mass (Da):74,666
Last modified:June 1, 1998 - v1
Checksum:i26803400A6836EF2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86332 mRNA Translation: BAA23667.1
BC047278 mRNA Translation: AAH47278.1
BC057952 mRNA Translation: AAH57952.1
CCDSiCCDS22561.1
RefSeqiNP_032635.1, NM_008609.4
UniGeneiMm.217116

Genome annotation databases

EnsembliENSMUST00000034243; ENSMUSP00000034243; ENSMUSG00000031790
GeneIDi17388
KEGGimmu:17388
UCSCiuc009myh.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86332 mRNA Translation: BAA23667.1
BC047278 mRNA Translation: AAH47278.1
BC057952 mRNA Translation: AAH57952.1
CCDSiCCDS22561.1
RefSeqiNP_032635.1, NM_008609.4
UniGeneiMm.217116

3D structure databases

ProteinModelPortaliO54732
SMRiO54732
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034243

Protein family/group databases

MEROPSiM10.015

PTM databases

iPTMnetiO54732
PhosphoSitePlusiO54732
SwissPalmiO54732

Proteomic databases

MaxQBiO54732
PaxDbiO54732
PeptideAtlasiO54732
PRIDEiO54732

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034243; ENSMUSP00000034243; ENSMUSG00000031790
GeneIDi17388
KEGGimmu:17388
UCSCiuc009myh.1 mouse

Organism-specific databases

CTDi4324
MGIiMGI:109320 Mmp15

Phylogenomic databases

eggNOGiKOG1565 Eukaryota
ENOG410XQ5D LUCA
GeneTreeiENSGT00760000118870
HOGENOMiHOG000217928
HOVERGENiHBG052484
InParanoidiO54732
KOiK07995
OMAiQHGDPGY
OrthoDBiEOG091G03DP
PhylomeDBiO54732
TreeFamiTF352396

Enzyme and pathway databases

BRENDAi3.4.24.B5 3474
ReactomeiR-MMU-1442490 Collagen degradation
R-MMU-1474228 Degradation of the extracellular matrix
R-MMU-1592389 Activation of Matrix Metalloproteinases

Miscellaneous databases

PROiPR:O54732
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031790 Expressed in 286 organ(s), highest expression level in decidua
CleanExiMM_MMP15
GenevisibleiO54732 MM

Family and domain databases

CDDicd00094 HX, 1 hit
cd04278 ZnMc_MMP, 1 hit
Gene3Di2.110.10.10, 1 hit
3.40.390.10, 1 hit
InterProiView protein in InterPro
IPR000585 Hemopexin-like_dom
IPR036375 Hemopexin-like_dom_sf
IPR018487 Hemopexin-like_repeat
IPR018486 Hemopexin_CS
IPR033739 M10A_MMP
IPR024079 MetalloPept_cat_dom_sf
IPR028729 MMP15
IPR001818 Pept_M10_metallopeptidase
IPR021190 Pept_M10A
IPR021805 Pept_M10A_metallopeptidase_C
IPR021158 Pept_M10A_Zn_BS
IPR006026 Peptidase_Metallo
IPR002477 Peptidoglycan-bd-like
IPR036365 PGBD-like_sf
PANTHERiPTHR10201:SF25 PTHR10201:SF25, 1 hit
PfamiView protein in Pfam
PF11857 DUF3377, 1 hit
PF00045 Hemopexin, 4 hits
PF00413 Peptidase_M10, 1 hit
PF01471 PG_binding_1, 1 hit
PIRSFiPIRSF001191 Peptidase_M10A_matrix, 1 hit
PRINTSiPR00138 MATRIXIN
SMARTiView protein in SMART
SM00120 HX, 4 hits
SM00235 ZnMc, 1 hit
SUPFAMiSSF47090 SSF47090, 1 hit
SSF50923 SSF50923, 1 hit
PROSITEiView protein in PROSITE
PS00546 CYSTEINE_SWITCH, 1 hit
PS00024 HEMOPEXIN, 1 hit
PS51642 HEMOPEXIN_2, 4 hits
PS00142 ZINC_PROTEASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMMP15_MOUSE
AccessioniPrimary (citable) accession number: O54732
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: June 1, 1998
Last modified: November 7, 2018
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again