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Protein

E3 SUMO-protein ligase PIAS3

Gene

Pias3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Repressor of STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Repressor of MITF transcriptional activity. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Sumoylates CCAR2 which promotes its interaction with SIRT1 (By similarity). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (By similarity).By similarity3 Publications

Pathwayi: protein sumoylation

This protein is involved in the pathway protein sumoylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri312 – 389SP-RING-typePROSITE-ProRule annotationAdd BLAST78

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processTranscription, Transcription regulation, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-3232118 SUMOylation of transcription factors
R-MMU-3899300 SUMOylation of transcription cofactors
R-MMU-4090294 SUMOylation of intracellular receptors
R-MMU-4615885 SUMOylation of DNA replication proteins
R-MMU-4755510 SUMOylation of immune response proteins
R-MMU-5696395 Formation of Incision Complex in GG-NER
UniPathwayi
UPA00886

Names & Taxonomyi

Protein namesi
Recommended name:
E3 SUMO-protein ligase PIAS3 (EC:2.3.2.-)
Alternative name(s):
E3 SUMO-protein transferase PIAS3Curated
Protein inhibitor of activated STAT protein 3
Gene namesi
Name:Pias3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1913126 Pias3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi19 – 23LQVLL → AQVAA: Nuclear and cytoplasmic location. 1 Publication5
Mutagenesisi141L → A: Greatly reduced interaction with STAT3. 1
Mutagenesisi143R → N: Abolishes interaction with STAT3. 1
Mutagenesisi143R → Q: Abolishes interaction with STAT3. 1
Mutagenesisi242 – 244INI → SDS: Nuclear and cytoplasmic location. 1 Publication3
Mutagenesisi343 – 348CAHLQS → GADLQG: Nuclear and cytoplasmic location. 1 Publication6
Mutagenesisi343C → S: Loss of promotion of IRF1 sumoylation, as well as of autosumoylation; partial loss of suppression of IRF1 transcriptional activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002189801 – 628E3 SUMO-protein ligase PIAS3Add BLAST628

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki46Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki56Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki230Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki307Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki466Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki482Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

Sumoylated.1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiO54714
PaxDbiO54714
PRIDEiO54714

PTM databases

PhosphoSitePlusiO54714

Expressioni

Tissue specificityi

Expressed in kidney, heart, spleen, brain and cerebellum; weak expression, if any, in liver and lung.2 Publications

Developmental stagei

Expressed as early as 7.6 dpc. Expression remains high through 15.5 dpc.1 Publication

Gene expression databases

BgeeiENSMUSG00000028101 Expressed in 279 organ(s), highest expression level in retina
CleanExiMM_PIAS3
ExpressionAtlasiO54714 baseline and differential
GenevisibleiO54714 MM

Interactioni

Subunit structurei

Monomer. Interacts with PLAG1 and ZFHX3. Interacts with STAT5A; the interaction occurs on stimulation by PRL (By similarity). Binds SUMO1 and UBE2I. Interacts with AR, BCL11A, HMGA2, IRF1 and NCOA2. Interacts with MITF; the interaction inhibits the transcriptional activity of MITF. Interacts with STAT3; the interaction occurs on stimulation by IL6, CNTF or OSM and inhibits the DNA binding activity of STAT3. Interacts with GFI1; the interaction relieves the inhibitory effect of PIAS3 on STAT3-mediated transcriptional activity. Interacts with MTA1. Interacts with CCAR2 (via N-terminus) (By similarity). Interacts with TRIM8 (By similarity).By similarity9 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Gfi1P703386EBI-927969,EBI-3954754

GO - Molecular functioni

Protein-protein interaction databases

BioGridi230874, 8 interactors
IntActiO54714, 2 interactors
STRINGi10090.ENSMUSP00000069259

Structurei

3D structure databases

ProteinModelPortaliO54714
SMRiO54714
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 45SAPPROSITE-ProRule annotationAdd BLAST35
Domaini115 – 280PINITPROSITE-ProRule annotationAdd BLAST166

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 200Interaction with CCAR2By similarityAdd BLAST200
Regioni450 – 460SUMO1-bindingBy similarityAdd BLAST11

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi19 – 23LXXLL motif5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi81 – 133Pro-richAdd BLAST53

Domaini

The LXXLL motif is a transcriptional coregulator signature.

Sequence similaritiesi

Belongs to the PIAS family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri312 – 389SP-RING-typePROSITE-ProRule annotationAdd BLAST78

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2169 Eukaryota
ENOG410XQ2E LUCA
GeneTreeiENSGT00550000074410
HOGENOMiHOG000230594
HOVERGENiHBG053598
InParanoidiO54714
KOiK16064
OMAiCRPDIIS
OrthoDBiEOG091G08G5
PhylomeDBiO54714
TreeFamiTF323787

Family and domain databases

Gene3Di1.10.720.30, 1 hit
2.60.120.780, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR027226 PIAS3
IPR023321 PINIT
IPR038654 PINIT_sf
IPR003034 SAP_dom
IPR036361 SAP_dom_sf
IPR004181 Znf_MIZ
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR10782:SF10 PTHR10782:SF10, 1 hit
PfamiView protein in Pfam
PF14324 PINIT, 1 hit
PF02891 zf-MIZ, 1 hit
SMARTiView protein in SMART
SM00513 SAP, 1 hit
SUPFAMiSSF68906 SSF68906, 1 hit
PROSITEiView protein in PROSITE
PS51466 PINIT, 1 hit
PS50800 SAP, 1 hit
PS51044 ZF_SP_RING, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O54714-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAELGELKHM VMSFRVSELQ VLLGFAGRNK SGRKHELLAK ALHLLKSSCA
60 70 80 90 100
PSVQMKIKEL YRRRFPRKTL GPSDLSLLSL PPGTSPVGSP GPLAPIPPTL
110 120 130 140 150
LTPGTLLGPK REVDMHPPLP QPVHPDVTMK PLPFYEVYGE LIRPTTLAST
160 170 180 190 200
SSQRFEEAHF TFALTPQQLQ QILTSREVLP GAKCDYTIQV QLRFCLCETS
210 220 230 240 250
CPQEDYFPPN LFVKVNGKLC PLPGYLPPTK NGAEPKRPSR PINITPLARL
260 270 280 290 300
SATVPNTIVV NWSSEFGRNY SLSVYLVRQL TAGTLLQKLR AKGIRNPDHS
310 320 330 340 350
RALIKEKLTA DPDSEVATTS LRVSLMCPLG KMRLTVPCRA LTCAHLQSFD
360 370 380 390 400
AALYLQMNEK KPTWTCPVCD KKAPYESLII DGLFMEILNS CSDCDEIQFM
410 420 430 440 450
EDGSWCPMKP KKEASEVCPP PGYGLDGLQY SAVQEGIQPE SKKRVEVIDL
460 470 480 490 500
TIESSSDEED LPPTKKHCPV TSAAIPALPG SKGALTSGHQ PSSVLRSPAM
510 520 530 540 550
GTLGSDFLSS LPLHEYPPAF PLGADIQGLD LFSFLQTESQ HYGPSVITSL
560 570 580 590 600
DEQDTLGHFF QYRGTPSHFL GPLAPTLGSS HRSSTPAPPP GRVSSIVAPG
610 620
SSLREGHGGP LPSGPSLTGC RSDVISLD
Length:628
Mass (Da):68,318
Last modified:December 7, 2004 - v3
Checksum:i2827F52715F0E549
GO
Isoform 2 (identifier: O54714-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.

Show »
Length:619
Mass (Da):67,309
Checksum:iF368B209502A47F3
GO
Isoform 3 (identifier: O54714-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     87-121: Missing.

Show »
Length:593
Mass (Da):64,741
Checksum:i651B188168468F47
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E0CZ31E0CZ31_MOUSE
E3 SUMO-protein ligase PIAS3
Pias3
430Annotation score:
E0CZH5E0CZH5_MOUSE
E3 SUMO-protein ligase PIAS3
Pias3
90Annotation score:
H3BJ41H3BJ41_MOUSE
E3 SUMO-protein ligase PIAS3
Pias3
52Annotation score:
H3BLM4H3BLM4_MOUSE
E3 SUMO-protein ligase PIAS3
Pias3
27Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0122031 – 9Missing in isoform 2. 2 Publications9
Alternative sequenceiVSP_01220487 – 121Missing in isoform 3. 1 PublicationAdd BLAST35

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK040619 mRNA Translation: BAC30647.1
AK077551 mRNA Translation: BAC36858.1
BC023128 mRNA Translation: AAH23128.1
BC051252 mRNA Translation: AAH51252.1
AF034080 mRNA Translation: AAB88902.3
CCDSiCCDS17645.2 [O54714-3]
CCDS38559.1 [O54714-2]
CCDS51007.1 [O54714-1]
RefSeqiNP_001159421.1, NM_001165949.1 [O54714-1]
NP_061282.2, NM_018812.2 [O54714-3]
NP_666247.1, NM_146135.2 [O54714-2]
XP_006501456.1, XM_006501393.1 [O54714-2]
UniGeneiMm.1635

Genome annotation databases

EnsembliENSMUST00000064900; ENSMUSP00000069259; ENSMUSG00000028101 [O54714-1]
ENSMUST00000107076; ENSMUSP00000102691; ENSMUSG00000028101 [O54714-2]
ENSMUST00000107077; ENSMUSP00000102692; ENSMUSG00000028101 [O54714-3]
GeneIDi229615
KEGGimmu:229615
UCSCiuc008qnt.2 mouse [O54714-1]
uc008qnu.2 mouse [O54714-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK040619 mRNA Translation: BAC30647.1
AK077551 mRNA Translation: BAC36858.1
BC023128 mRNA Translation: AAH23128.1
BC051252 mRNA Translation: AAH51252.1
AF034080 mRNA Translation: AAB88902.3
CCDSiCCDS17645.2 [O54714-3]
CCDS38559.1 [O54714-2]
CCDS51007.1 [O54714-1]
RefSeqiNP_001159421.1, NM_001165949.1 [O54714-1]
NP_061282.2, NM_018812.2 [O54714-3]
NP_666247.1, NM_146135.2 [O54714-2]
XP_006501456.1, XM_006501393.1 [O54714-2]
UniGeneiMm.1635

3D structure databases

ProteinModelPortaliO54714
SMRiO54714
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230874, 8 interactors
IntActiO54714, 2 interactors
STRINGi10090.ENSMUSP00000069259

PTM databases

PhosphoSitePlusiO54714

Proteomic databases

MaxQBiO54714
PaxDbiO54714
PRIDEiO54714

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064900; ENSMUSP00000069259; ENSMUSG00000028101 [O54714-1]
ENSMUST00000107076; ENSMUSP00000102691; ENSMUSG00000028101 [O54714-2]
ENSMUST00000107077; ENSMUSP00000102692; ENSMUSG00000028101 [O54714-3]
GeneIDi229615
KEGGimmu:229615
UCSCiuc008qnt.2 mouse [O54714-1]
uc008qnu.2 mouse [O54714-3]

Organism-specific databases

CTDi10401
MGIiMGI:1913126 Pias3

Phylogenomic databases

eggNOGiKOG2169 Eukaryota
ENOG410XQ2E LUCA
GeneTreeiENSGT00550000074410
HOGENOMiHOG000230594
HOVERGENiHBG053598
InParanoidiO54714
KOiK16064
OMAiCRPDIIS
OrthoDBiEOG091G08G5
PhylomeDBiO54714
TreeFamiTF323787

Enzyme and pathway databases

UniPathwayi
UPA00886

ReactomeiR-MMU-3232118 SUMOylation of transcription factors
R-MMU-3899300 SUMOylation of transcription cofactors
R-MMU-4090294 SUMOylation of intracellular receptors
R-MMU-4615885 SUMOylation of DNA replication proteins
R-MMU-4755510 SUMOylation of immune response proteins
R-MMU-5696395 Formation of Incision Complex in GG-NER

Miscellaneous databases

ChiTaRSiPias3 mouse
PROiPR:O54714
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028101 Expressed in 279 organ(s), highest expression level in retina
CleanExiMM_PIAS3
ExpressionAtlasiO54714 baseline and differential
GenevisibleiO54714 MM

Family and domain databases

Gene3Di1.10.720.30, 1 hit
2.60.120.780, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR027226 PIAS3
IPR023321 PINIT
IPR038654 PINIT_sf
IPR003034 SAP_dom
IPR036361 SAP_dom_sf
IPR004181 Znf_MIZ
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR10782:SF10 PTHR10782:SF10, 1 hit
PfamiView protein in Pfam
PF14324 PINIT, 1 hit
PF02891 zf-MIZ, 1 hit
SMARTiView protein in SMART
SM00513 SAP, 1 hit
SUPFAMiSSF68906 SSF68906, 1 hit
PROSITEiView protein in PROSITE
PS51466 PINIT, 1 hit
PS50800 SAP, 1 hit
PS51044 ZF_SP_RING, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPIAS3_MOUSE
AccessioniPrimary (citable) accession number: O54714
Secondary accession number(s): Q80WF8, Q8R598
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: December 7, 2004
Last modified: November 7, 2018
This is version 156 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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