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Protein

Centromere/kinetochore protein zw10 homolog

Gene

Zw10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex. Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum (ER); the function is proposed to depend on its association in the interphase NRZ complex which is believed to play a role in SNARE assembly at the ER (By similarity).By similarity

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, ER-Golgi transport, Mitosis, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-MMU-68877 Mitotic Prometaphase

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere/kinetochore protein zw10 homolog
Gene namesi
Name:Zw10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1349478 Zw10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Kinetochore, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001849582 – 779Centromere/kinetochore protein zw10 homologAdd BLAST778

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei3PhosphoserineBy similarity1
Modified residuei12PhosphoserineBy similarity1
Modified residuei777N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO54692
MaxQBiO54692
PaxDbiO54692
PeptideAtlasiO54692
PRIDEiO54692

PTM databases

iPTMnetiO54692
PhosphoSitePlusiO54692

Expressioni

Gene expression databases

BgeeiENSMUSG00000032264 Expressed in 277 organ(s), highest expression level in primary oocyte
ExpressionAtlasiO54692 baseline and differential
GenevisibleiO54692 MM

Interactioni

Subunit structurei

Interacts with NBAS and KNTC1/ROD; the interactions are mutually exclusive and indicative for its association in two different vesicle tethering complexes. Component of the RZZ complex composed of KNTC1/ROD, ZW10 and ZWILCH. Component of the NRZ complex composed of NBAS, ZW10 and RINT1/TIP20L; NRZ associates with SNAREs STX18, USE1L, BNIP1/SEC20L and SEC22B (the assembly has been described as syntaxin 18 complex). Interacts directly with RINT1/TIP20L bound to BNIP1/SEC20L. Interacts with C19orf25 and ZWINT STOPPED Associated with a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L, and SEC22B through direct interaction with RINT1/TIP20L bound to BNIP1/SEC20L. Component of the RZZ complex composed of KNTC1/ROD, ZW10 and ZWILCH. Interacts with C19orf25, KNTC1 and ZWINT.By similarity

Protein-protein interaction databases

BioGridi205086, 18 interactors
IntActiO54692, 18 interactors
MINTiO54692
STRINGi10090.ENSMUSP00000034803

Structurei

3D structure databases

ProteinModelPortaliO54692
SMRiO54692
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 317Interaction with RINT1By similarityAdd BLAST316
Regioni2 – 81Interaction with ZWINTBy similarityAdd BLAST80

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili14 – 130Sequence analysisAdd BLAST117

Sequence similaritiesi

Belongs to the ZW10 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2163 Eukaryota
ENOG410XQ7J LUCA
GeneTreeiENSGT00390000016427
HOGENOMiHOG000007982
HOVERGENiHBG004603
InParanoidiO54692
KOiK11578
OMAiRFESVMT
OrthoDBiEOG091G026L
PhylomeDBiO54692
TreeFamiTF105966

Family and domain databases

InterProiView protein in InterPro
IPR009361 RZZ-complex_Zw10
PANTHERiPTHR12205 PTHR12205, 1 hit
PfamiView protein in Pfam
PF06248 Zw10, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O54692-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASFVTEVLA HSGSLEKEDL GTRISRLTRR VEEIKGEVCN MISKKYSEFL
60 70 80 90 100
PTMQSAQALV TQVDTLSNDI DQLKSRIETE VCRDLHISTV EFTNLKQQLE
110 120 130 140 150
RDSVVLTLLK QLQEFSSAIE EYNSALAEKK YIPAARHLEE AQECLKLLKS
160 170 180 190 200
RKCFDLKMLK SLSMELTVQK QNILYHLGED WQKLVVWKFP PAKDTSSLES
210 220 230 240 250
CLQTELHLCT EQPEKEDMTP LPSISSVLLA FSILGELPTK LKSFGQMLLK
260 270 280 290 300
YILKPLVTCP SLHAVIERQP SSVSICFESL TTDLEHPSPP EAFAKIRLVL
310 320 330 340 350
EVLQKQLLDL PLDADLEIGK VPGIVLAEML GEGIWEDLSE CLIRNCLVYS
360 370 380 390 400
IPTNSSKLQE YEEIIQSTEE FEKFLKEMRF LKGDTTDLLK YARNINSHFA
410 420 430 440 450
NKKCQDVIVA ARNLMTSEIH NTVKIGPDCK EALPDLPSPD ADHKLQVQTV
460 470 480 490 500
CKAQFTDAGN LEPETSLDPQ SFSLPTCRIS EAVKKLMELA YQTLLEATTS
510 520 530 540 550
SDQCAVQLFY SVRNIFHLFH DVVPTYHKEN LRKLPQLAAI HHNNCMYIAH
560 570 580 590 600
HLLTLGHQFR LRLAPILCDG TTTFVDLVPG FRRLGTECFL AQMQAQKGEL
610 620 630 640 650
LERLSSARSF ANMDDEENYS AASKAVRQVL HQLRRLGIVW QDVLPVNIYC
660 670 680 690 700
KAMGTLLNTA IAEMMSRITA LEDISTEDGD RLYSLCKTVM DEGPQVFAPL
710 720 730 740 750
SDENKNKKYQ EEVPVYVSKW MPFKELMIML QASLQEIGDR WADGKGPLAT
760 770
AFPSSEVKAL IRALFQNTER RAAALAKIK
Length:779
Mass (Da):88,063
Last modified:January 23, 2007 - v3
Checksum:i5C02CF77FDF5B13B
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1L1SST9A0A1L1SST9_MOUSE
Centromere/kinetochore protein zw10...
Zw10
187Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti143E → A in BAE26337 (PubMed:16141072).Curated1
Sequence conflicti281T → A in BAE26337 (PubMed:16141072).Curated1
Sequence conflicti281T → A in BAE39941 (PubMed:16141072).Curated1
Sequence conflicti292A → T in BAE39941 (PubMed:16141072).Curated1
Sequence conflicti449T → K in BAE26337 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK145270 mRNA Translation: BAE26337.1
AK167251 mRNA Translation: BAE39372.1
AK167937 mRNA Translation: BAE39941.1
AK168479 mRNA Translation: BAE40368.1
BC012435 mRNA Translation: AAH12435.1
AF003951 mRNA Translation: AAB88256.1
CCDSiCCDS23161.1
RefSeqiNP_036169.1, NM_012039.2
UniGeneiMm.24791

Genome annotation databases

EnsembliENSMUST00000034803; ENSMUSP00000034803; ENSMUSG00000032264
GeneIDi26951
KEGGimmu:26951
UCSCiuc009piu.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK145270 mRNA Translation: BAE26337.1
AK167251 mRNA Translation: BAE39372.1
AK167937 mRNA Translation: BAE39941.1
AK168479 mRNA Translation: BAE40368.1
BC012435 mRNA Translation: AAH12435.1
AF003951 mRNA Translation: AAB88256.1
CCDSiCCDS23161.1
RefSeqiNP_036169.1, NM_012039.2
UniGeneiMm.24791

3D structure databases

ProteinModelPortaliO54692
SMRiO54692
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205086, 18 interactors
IntActiO54692, 18 interactors
MINTiO54692
STRINGi10090.ENSMUSP00000034803

PTM databases

iPTMnetiO54692
PhosphoSitePlusiO54692

Proteomic databases

EPDiO54692
MaxQBiO54692
PaxDbiO54692
PeptideAtlasiO54692
PRIDEiO54692

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034803; ENSMUSP00000034803; ENSMUSG00000032264
GeneIDi26951
KEGGimmu:26951
UCSCiuc009piu.2 mouse

Organism-specific databases

CTDi9183
MGIiMGI:1349478 Zw10

Phylogenomic databases

eggNOGiKOG2163 Eukaryota
ENOG410XQ7J LUCA
GeneTreeiENSGT00390000016427
HOGENOMiHOG000007982
HOVERGENiHBG004603
InParanoidiO54692
KOiK11578
OMAiRFESVMT
OrthoDBiEOG091G026L
PhylomeDBiO54692
TreeFamiTF105966

Enzyme and pathway databases

ReactomeiR-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-MMU-68877 Mitotic Prometaphase

Miscellaneous databases

PROiPR:O54692
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032264 Expressed in 277 organ(s), highest expression level in primary oocyte
ExpressionAtlasiO54692 baseline and differential
GenevisibleiO54692 MM

Family and domain databases

InterProiView protein in InterPro
IPR009361 RZZ-complex_Zw10
PANTHERiPTHR12205 PTHR12205, 1 hit
PfamiView protein in Pfam
PF06248 Zw10, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiZW10_MOUSE
AccessioniPrimary (citable) accession number: O54692
Secondary accession number(s): Q3TIA5, Q3ULW1, Q921H3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 138 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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