UniProtKB - O54689 (CCR6_MOUSE)
Protein
C-C chemokine receptor type 6
Gene
Ccr6
Organism
Mus musculus (Mouse)
Status
Functioni
Receptor for the C-C type chemokine CCL20. Binds to CCL20 and subsequently transduces a signal by increasing the intracellular calcium ion levels (PubMed:20068036). Although CCL20 is its major ligand it can also act as a receptor for non-chemokine ligands such as beta-defensins (PubMed:25122636). Binds to defensin DEFB1 leading to increase in intracellular calcium ions and cAMP levels. Its binding to DEFB1 is essential for the function of DEFB1 in regulating sperm motility and bactericidal activity (By similarity). Binds to defensins DEFB4 and DEFB4A/B and mediates their chemotactic effects (PubMed:20068036). The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and various autoimmune diseases. CCR6-mediated signals are essential for immune responses to microbes in the intestinal mucosa and in the modulation of inflammatory responses initiated by tissue insult and trauma (PubMed:21376174). CCR6 is essential for the recruitment of both the proinflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation (PubMed:19050256). Required for the normal migration of Th17 cells in Peyers patches and other related tissue sites of the intestine and plays a role in regulating effector T-cell balance and distribution in inflamed intestine (PubMed:19129757). Plays an important role in the coordination of early thymocyte precursor migration events important for normal subsequent thymocyte precursor development, but is not required for the formation of normal thymic natural regulatory T-cells (nTregs). Required for optimal differentiation of DN2 and DN3 thymocyte precursors (PubMed:24638065). Essential for B-cell localization in the subepithelial dome of Peyers-patches and for efficient B-cell isotype switching to IgA in the Peyers-patches (PubMed:27174992). Essential for appropriate anatomical distribution of memory B-cells in the spleen and for the secondary recall response of memory B-cells (PubMed:25505290). Positively regulates sperm motility and chemotaxis via its binding to CCL20 (PubMed:23765988).1 PublicationBy similarity8 Publications
GO - Molecular functioni
- C-C chemokine binding Source: UniProtKB
- C-C chemokine receptor activity Source: UniProtKB
- chemokine binding Source: GO_Central
GO - Biological processi
- calcium-mediated signaling Source: UniProtKB
- cell chemotaxis Source: UniProtKB
- chemotaxis Source: UniProtKB
- DN2 thymocyte differentiation Source: UniProtKB
- DN3 thymocyte differentiation Source: UniProtKB
- immune response Source: GO_Central
- isotype switching to IgA isotypes Source: UniProtKB
- leukocyte migration involved in inflammatory response Source: UniProtKB
- lymphocyte migration Source: UniProtKB
- positive regulation of cytosolic calcium ion concentration Source: MGI
- positive regulation of dendritic cell chemotaxis Source: MGI
- positive regulation of epithelial cell migration Source: MGI
- positive regulation of flagellated sperm motility involved in capacitation Source: UniProtKB
- regulation of T cell migration Source: UniProtKB
- T cell migration Source: UniProtKB
- thymocyte migration Source: UniProtKB
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-MMU-1461957, Beta defensins R-MMU-380108, Chemokine receptors bind chemokines R-MMU-418594, G alpha (i) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: C-C chemokine receptor type 6Short name: C-C CKR-6 Short name: CC-CKR-6 Short name: CCR-6 Alternative name(s): KY411 CD_antigen: CD196 |
Gene namesi | Name:Ccr6 Synonyms:Cmkbr6 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1333797, Ccr6 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Other locations
- Cell surface By similarity
Plasma Membrane
- external side of plasma membrane Source: MGI
- sperm plasma membrane Source: UniProtKB
Other locations
- cell surface Source: MGI
- integral component of membrane Source: UniProtKB-KW
- sperm flagellum Source: UniProtKB
- sperm midpiece Source: MGI
- sperm principal piece Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 39 | ExtracellularSequence analysisAdd BLAST | 39 | |
Transmembranei | 40 – 66 | Helical; Name=1Sequence analysisAdd BLAST | 27 | |
Topological domaini | 67 – 75 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 76 – 96 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 97 – 111 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 112 – 133 | Helical; Name=3Sequence analysisAdd BLAST | 22 | |
Topological domaini | 134 – 151 | CytoplasmicSequence analysisAdd BLAST | 18 | |
Transmembranei | 152 – 172 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 173 – 203 | ExtracellularSequence analysisAdd BLAST | 31 | |
Transmembranei | 204 – 230 | Helical; Name=5Sequence analysisAdd BLAST | 27 | |
Topological domaini | 231 – 246 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 247 – 271 | Helical; Name=6Sequence analysisAdd BLAST | 25 | |
Topological domaini | 272 – 295 | ExtracellularSequence analysisAdd BLAST | 24 | |
Transmembranei | 296 – 313 | Helical; Name=7Sequence analysisAdd BLAST | 18 | |
Topological domaini | 314 – 367 | CytoplasmicSequence analysisAdd BLAST | 54 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000069287 | 1 – 367 | C-C chemokine receptor type 6Add BLAST | 367 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 2 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 35 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 110 ↔ 189 | PROSITE-ProRule annotationBy similarity |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | O54689 |
PRIDEi | O54689 |
PTM databases
GlyGeni | O54689, 2 sites |
iPTMneti | O54689 |
PhosphoSitePlusi | O54689 |
Expressioni
Tissue specificityi
Sperm. Mainly localized in the principal piece and neck region of the tail but is also found in the acrosomal region in a small percentage of sperm cells. Expressed in natural regulatory T cells (nTregs) and a subset of early thymocyte progenitor double-negative 1 (DN1) cells. Expressed in memory B cells. Expressed by IL17 producing helper T-cells (Th17), type 1 effector cells (Th1), type 2 effector cells (Th2) and regulatory T-cells (Treg) (at protein level). Expressed by Th17 cells in spleen, Peyers patches, and lamina propria of small and large intestine. Highly expressed in testis, lung, colon, and dendritic cells.5 Publications
Inductioni
Up-regulated on pre-germinal center B-cells in a CD40-dependent manner. Up-regulated and down-regulated in Th17 cells by TGFB1 and IL2 respectively.3 Publications
Gene expression databases
Bgeei | ENSMUSG00000040899, Expressed in subcutaneous adipose tissue and 95 other tissues |
ExpressionAtlasi | O54689, baseline and differential |
Genevisiblei | O54689, MM |
Interactioni
GO - Molecular functioni
- C-C chemokine binding Source: UniProtKB
- chemokine binding Source: GO_Central
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000095029 |
Miscellaneous databases
RNActi | O54689, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01000000214381 |
HOGENOMi | CLU_009579_8_3_1 |
InParanoidi | O54689 |
OMAi | VFMIFCY |
OrthoDBi | 981680at2759 |
PhylomeDBi | O54689 |
TreeFami | TF330966 |
Family and domain databases
InterProi | View protein in InterPro IPR004067, Chemokine_CCR6 IPR000355, Chemokine_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
PANTHERi | PTHR10489:SF611, PTHR10489:SF611, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00657, CCCHEMOKINER PR01529, CHEMOKINER6 PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O54689-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNSTESYFGT DDYDNTEYYS IPPDHGPCSL EEVRNFTKVF VPIAYSLICV
60 70 80 90 100
FGLLGNIMVV MTFAFYKKAR SMTDVYLLNM AITDILFVLT LPFWAVTHAT
110 120 130 140 150
NTWVFSDALC KLMKGTYAVN FNCGMLLLAC ISMDRYIAIV QATKSFRVRS
160 170 180 190 200
RTLTHSKVIC VAVWFISIII SSPTFIFNKK YELQDRDVCE PRYRSVSEPI
210 220 230 240 250
TWKLLGMGLE LFFGFFTPLL FMVFCYLFII KTLVQAQNSK RHRAIRVVIA
260 270 280 290 300
VVLVFLACQI PHNMVLLVTA VNTGKVGRSC STEKVLAYTR NVAEVLAFLH
310 320 330 340 350
CCLNPVLYAF IGQKFRNYFM KIMKDVWCMR RKNKMPGFLC ARVYSESYIS
360
RQTSETVEND NASSFTM
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A338P732 | A0A338P732_MOUSE | C-C chemokine receptor type 6 | Ccr6 | 48 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB009369 mRNA Translation: BAA23776.1 AJ222714 Genomic DNA Translation: CAA10956.1 |
CCDSi | CCDS28381.1 |
RefSeqi | NP_001177262.1, NM_001190333.1 NP_001177263.1, NM_001190334.1 NP_001177264.1, NM_001190335.1 NP_001177265.1, NM_001190336.1 NP_001177266.1, NM_001190337.1 NP_033965.1, NM_009835.4 XP_006523349.1, XM_006523286.3 XP_006523351.1, XM_006523288.3 XP_006523353.1, XM_006523290.3 XP_006523354.1, XM_006523291.1 XP_006523355.1, XM_006523292.3 XP_011244488.1, XM_011246186.2 XP_011244490.1, XM_011246188.1 XP_017172707.1, XM_017317218.1 XP_017172708.1, XM_017317219.1 XP_017172709.1, XM_017317220.1 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB009369 mRNA Translation: BAA23776.1 AJ222714 Genomic DNA Translation: CAA10956.1 |
CCDSi | CCDS28381.1 |
RefSeqi | NP_001177262.1, NM_001190333.1 NP_001177263.1, NM_001190334.1 NP_001177264.1, NM_001190335.1 NP_001177265.1, NM_001190336.1 NP_001177266.1, NM_001190337.1 NP_033965.1, NM_009835.4 XP_006523349.1, XM_006523286.3 XP_006523351.1, XM_006523288.3 XP_006523353.1, XM_006523290.3 XP_006523354.1, XM_006523291.1 XP_006523355.1, XM_006523292.3 XP_011244488.1, XM_011246186.2 XP_011244490.1, XM_011246188.1 XP_017172707.1, XM_017317218.1 XP_017172708.1, XM_017317219.1 XP_017172709.1, XM_017317220.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000095029 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | O54689, 2 sites |
iPTMneti | O54689 |
PhosphoSitePlusi | O54689 |
Proteomic databases
PaxDbi | O54689 |
PRIDEi | O54689 |
Protocols and materials databases
Antibodypediai | 2930, 810 antibodies |
Genome annotation databases
Organism-specific databases
CTDi | 1235 |
MGIi | MGI:1333797, Ccr6 |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01000000214381 |
HOGENOMi | CLU_009579_8_3_1 |
InParanoidi | O54689 |
OMAi | VFMIFCY |
OrthoDBi | 981680at2759 |
PhylomeDBi | O54689 |
TreeFami | TF330966 |
Enzyme and pathway databases
Reactomei | R-MMU-1461957, Beta defensins R-MMU-380108, Chemokine receptors bind chemokines R-MMU-418594, G alpha (i) signalling events |
Miscellaneous databases
BioGRID-ORCSi | 12458, 0 hits in 17 CRISPR screens |
PROi | PR:O54689 |
RNActi | O54689, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000040899, Expressed in subcutaneous adipose tissue and 95 other tissues |
ExpressionAtlasi | O54689, baseline and differential |
Genevisiblei | O54689, MM |
Family and domain databases
InterProi | View protein in InterPro IPR004067, Chemokine_CCR6 IPR000355, Chemokine_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
PANTHERi | PTHR10489:SF611, PTHR10489:SF611, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00657, CCCHEMOKINER PR01529, CHEMOKINER6 PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CCR6_MOUSE | |
Accessioni | O54689Primary (citable) accession number: O54689 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | June 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries