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Entry version 141 (16 Oct 2019)
Sequence version 3 (07 Jul 2009)
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Protein

GTP pyrophosphokinase

Gene

relA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp, it is probably the hydrolysis activity that is required for optimal growth (Probable).By similarity1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: ppGpp biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes ppGpp from GTP.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. GTP pyrophosphokinase (relA), GTP pyrophosphokinase YjbM (yjbM), GTP pyrophosphokinase YwaC (ywaC)
  2. no protein annotated in this organism
This subpathway is part of the pathway ppGpp biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ppGpp from GTP, the pathway ppGpp biosynthesis and in Purine metabolism.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
BSUB:BSU27600-MONOMER

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00908;UER00884

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
GTP pyrophosphokinase (EC:2.7.6.5)
Alternative name(s):
(p)ppGpp synthase
ATP:GTP 3'-pyrophosphotransferase
ppGpp synthase I
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:relA
Ordered Locus Names:BSU27600
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiBacillus subtilis (strain 168)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri224308 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001570 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Decreased growth rate; growth is almost completely restored in a triple relA-yjbM-ywaC mutant (PubMed:18067544).1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi264D → G: Wild-type growth rate, this enzyme probably has no synthase activity but can still degrade pppGpp to ppGpp. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001665441 – 734GTP pyrophosphokinaseAdd BLAST734

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
O54408

PRoteomics IDEntifications database

More...
PRIDEi
O54408

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

Protein interaction database and analysis system

More...
IntActi
O54408, 1 interactor

STRING: functional protein association networks

More...
STRINGi
224308.BSU27600

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
O54408

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini50 – 149HDPROSITE-ProRule annotationAdd BLAST100
Domaini392 – 453TGSPROSITE-ProRule annotationAdd BLAST62
Domaini660 – 734ACTPROSITE-ProRule annotationAdd BLAST75

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the RelA/SpoT family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4105CWR Bacteria
COG0317 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000018299

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
O54408

KEGG Orthology (KO)

More...
KOi
K00951

Identification of Orthologs from Complete Genome Data

More...
OMAi
HYYSIYQ

Database for complete collections of gene phylogenies

More...
PhylomeDBi
O54408

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00077 HDc, 1 hit
cd05399 NT_Rel-Spo_like, 1 hit
cd01668 TGS_RSH, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.10.20.30, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR002912 ACT_dom
IPR012675 Beta-grasp_dom_sf
IPR003607 HD/PDEase_dom
IPR004811 RelA/Spo_fam
IPR007685 RelA_SpoT
IPR004095 TGS
IPR012676 TGS-like
IPR033655 TGS_RelA

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF13291 ACT_4, 1 hit
PF04607 RelA_SpoT, 1 hit
PF02824 TGS, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00471 HDc, 1 hit
SM00954 RelA_SpoT, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF81271 SSF81271, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00691 spoT_relA, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51671 ACT, 1 hit
PS51831 HD, 1 hit
PS51880 TGS, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

O54408-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MANEQVLTAE QVIDKARSYL SDEHIAFVEK AYLYAEDAHR EQYRKSGEPY
60 70 80 90 100
IIHPIQVAGI LVDLEMDPST IAGGFLHDVV EDTDVTLDDL KEAFSEEVAM
110 120 130 140 150
LVDGVTKLGK IKYKSQEEQQ AENHRKMFVA MAQDIRVILI KLADRLHNMR
160 170 180 190 200
TLKHLPQEKQ RRISNETLEI FAPLAHRLGI SKIKWELEDT ALRYLNPQQY
210 220 230 240 250
YRIVNLMKKK RAERELYVDE VVNEVKKRVE EVNIKADFSG RPKHIYSIYR
260 270 280 290 300
KMVLQNKQFN EIYDLLAVRI LVNSIKDCYA VLGIIHTCWK PMPGRFKDYI
310 320 330 340 350
AMPKPNMYQS LHTTVIGPKG DPLEVQIRTF EMHEIAEYGV AAHWAYKEGK
360 370 380 390 400
AANEGATFEK KLSWFREILE FQNESTDAEE FMESLKIDLF SDMVYVFTPK
410 420 430 440 450
GDVIELPSGS VPIDFSYRIH SEIGNKTIGA KVNGKMVTLD HKLRTGDIVE
460 470 480 490 500
ILTSKHSYGP SQDWVKLAQT SQAKHKIRQF FKKQRREENV EKGRELVEKE
510 520 530 540 550
IKNLDFELKD VLTPENIQKV ADKFNFSNEE DMYAAVGYNG ITALQVANRL
560 570 580 590 600
TEKERKQRDQ EEQEKIVQEV TGEPKPYPQG RKREAGVRVK GIDNLLVRLS
610 620 630 640 650
KCCNPVPGDD IVGFITKGRG VSVHREDCPN VKTNEAQERL IPVEWEHESQ
660 670 680 690 700
VQKRKEYNVE IEILGYDRRG LLNEVLQAVN ETKTNISSVS GKSDRNKVAT
710 720 730
IHMAIFIQNI NHLHKVVERI KQIRDIYSVR RVMN
Length:734
Mass (Da):84,824
Last modified:July 7, 2009 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i6F06A3E0B9FFAB36
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U86377 Genomic DNA Translation: AAC46041.1
AL009126 Genomic DNA Translation: CAB14719.2

Protein sequence database of the Protein Information Resource

More...
PIRi
C69691

NCBI Reference Sequences

More...
RefSeqi
NP_390638.2, NC_000964.3
WP_003229747.1, NZ_JNCM01000036.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
CAB14719; CAB14719; BSU27600

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
936753

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
bsu:BSU27600

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|224308.179.peg.2999

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86377 Genomic DNA Translation: AAC46041.1
AL009126 Genomic DNA Translation: CAB14719.2
PIRiC69691
RefSeqiNP_390638.2, NC_000964.3
WP_003229747.1, NZ_JNCM01000036.1

3D structure databases

SMRiO54408
ModBaseiSearch...

Protein-protein interaction databases

IntActiO54408, 1 interactor
STRINGi224308.BSU27600

Proteomic databases

PaxDbiO54408
PRIDEiO54408

Genome annotation databases

EnsemblBacteriaiCAB14719; CAB14719; BSU27600
GeneIDi936753
KEGGibsu:BSU27600
PATRICifig|224308.179.peg.2999

Phylogenomic databases

eggNOGiENOG4105CWR Bacteria
COG0317 LUCA
HOGENOMiHOG000018299
InParanoidiO54408
KOiK00951
OMAiHYYSIYQ
PhylomeDBiO54408

Enzyme and pathway databases

UniPathwayiUPA00908;UER00884
BioCyciBSUB:BSU27600-MONOMER

Family and domain databases

CDDicd00077 HDc, 1 hit
cd05399 NT_Rel-Spo_like, 1 hit
cd01668 TGS_RSH, 1 hit
Gene3Di3.10.20.30, 1 hit
InterProiView protein in InterPro
IPR002912 ACT_dom
IPR012675 Beta-grasp_dom_sf
IPR003607 HD/PDEase_dom
IPR004811 RelA/Spo_fam
IPR007685 RelA_SpoT
IPR004095 TGS
IPR012676 TGS-like
IPR033655 TGS_RelA
PfamiView protein in Pfam
PF13291 ACT_4, 1 hit
PF04607 RelA_SpoT, 1 hit
PF02824 TGS, 1 hit
SMARTiView protein in SMART
SM00471 HDc, 1 hit
SM00954 RelA_SpoT, 1 hit
SUPFAMiSSF81271 SSF81271, 1 hit
TIGRFAMsiTIGR00691 spoT_relA, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 1 hit
PS51831 HD, 1 hit
PS51880 TGS, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiRELA_BACSU
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O54408
Secondary accession number(s): O32043
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 7, 2009
Last modified: October 16, 2019
This is version 141 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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