UniProtKB - O54408 (RELA_BACSU)
Protein
GTP pyrophosphokinase
Gene
relA
Organism
Bacillus subtilis (strain 168)
Status
Functioni
In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp, it is probably the hydrolysis activity that is required for optimal growth (Probable).By similarity1 Publication
Catalytic activityi
- EC:2.7.6.5
: ppGpp biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes ppGpp from GTP.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- GTP diphosphokinase (yjbM), GTP pyrophosphokinase (relA), GTP pyrophosphokinase YjbM (yjbM), GTP pyrophosphokinase YwaC (ywaC), (p)ppGpp synthase (relA), GTP pyrophosphokinase YwaC (ywaC)
- no protein annotated in this organism
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ppGpp from GTP, the pathway ppGpp biosynthesis and in Purine metabolism.
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- GTP binding Source: UniProtKB-KW
- GTP diphosphokinase activity Source: UniProtKB-EC
- kinase activity Source: UniProtKB-KW
GO - Biological processi
- guanosine tetraphosphate biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Kinase, Transferase |
Ligand | ATP-binding, GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU27600-MONOMER |
UniPathwayi | UPA00908;UER00884 |
Names & Taxonomyi
Protein namesi | Recommended name: GTP pyrophosphokinase (EC:2.7.6.5)Alternative name(s): (p)ppGpp synthase ATP:GTP 3'-pyrophosphotransferase ppGpp synthase I |
Gene namesi | Name:relA Ordered Locus Names:BSU27600 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Decreased growth rate; growth is almost completely restored in a triple relA-yjbM-ywaC mutant (PubMed:18067544).1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 264 | D → G: Wild-type growth rate, this enzyme probably has no synthase activity but can still degrade pppGpp to ppGpp. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000166544 | 1 – 734 | GTP pyrophosphokinaseAdd BLAST | 734 |
Proteomic databases
jPOSTi | O54408 |
PaxDbi | O54408 |
PRIDEi | O54408 |
Interactioni
Protein-protein interaction databases
IntActi | O54408, 1 interactor |
STRINGi | 224308.BSU27600 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 50 – 149 | HDPROSITE-ProRule annotationAdd BLAST | 100 | |
Domaini | 392 – 453 | TGSPROSITE-ProRule annotationAdd BLAST | 62 | |
Domaini | 660 – 734 | ACTPROSITE-ProRule annotationAdd BLAST | 75 |
Sequence similaritiesi
Belongs to the RelA/SpoT family.Curated
Phylogenomic databases
eggNOGi | COG0317, Bacteria |
InParanoidi | O54408 |
OMAi | HYYSIYQ |
PhylomeDBi | O54408 |
Family and domain databases
CDDi | cd00077, HDc, 1 hit cd05399, NT_Rel-Spo_like, 1 hit cd01668, TGS_RSH, 1 hit |
Gene3Di | 3.10.20.30, 1 hit 3.30.460.10, 1 hit |
InterProi | View protein in InterPro IPR002912, ACT_dom IPR012675, Beta-grasp_dom_sf IPR003607, HD/PDEase_dom IPR043519, NT_sf IPR004811, RelA/Spo_fam IPR007685, RelA_SpoT IPR004095, TGS IPR012676, TGS-like IPR033655, TGS_RelA |
Pfami | View protein in Pfam PF13291, ACT_4, 1 hit PF04607, RelA_SpoT, 1 hit PF02824, TGS, 1 hit |
SMARTi | View protein in SMART SM00471, HDc, 1 hit SM00954, RelA_SpoT, 1 hit |
SUPFAMi | SSF81271, SSF81271, 1 hit SSF81301, SSF81301, 1 hit |
TIGRFAMsi | TIGR00691, spoT_relA, 1 hit |
PROSITEi | View protein in PROSITE PS51671, ACT, 1 hit PS51831, HD, 1 hit PS51880, TGS, 1 hit |
i Sequence
Sequence statusi: Complete.
O54408-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MANEQVLTAE QVIDKARSYL SDEHIAFVEK AYLYAEDAHR EQYRKSGEPY
60 70 80 90 100
IIHPIQVAGI LVDLEMDPST IAGGFLHDVV EDTDVTLDDL KEAFSEEVAM
110 120 130 140 150
LVDGVTKLGK IKYKSQEEQQ AENHRKMFVA MAQDIRVILI KLADRLHNMR
160 170 180 190 200
TLKHLPQEKQ RRISNETLEI FAPLAHRLGI SKIKWELEDT ALRYLNPQQY
210 220 230 240 250
YRIVNLMKKK RAERELYVDE VVNEVKKRVE EVNIKADFSG RPKHIYSIYR
260 270 280 290 300
KMVLQNKQFN EIYDLLAVRI LVNSIKDCYA VLGIIHTCWK PMPGRFKDYI
310 320 330 340 350
AMPKPNMYQS LHTTVIGPKG DPLEVQIRTF EMHEIAEYGV AAHWAYKEGK
360 370 380 390 400
AANEGATFEK KLSWFREILE FQNESTDAEE FMESLKIDLF SDMVYVFTPK
410 420 430 440 450
GDVIELPSGS VPIDFSYRIH SEIGNKTIGA KVNGKMVTLD HKLRTGDIVE
460 470 480 490 500
ILTSKHSYGP SQDWVKLAQT SQAKHKIRQF FKKQRREENV EKGRELVEKE
510 520 530 540 550
IKNLDFELKD VLTPENIQKV ADKFNFSNEE DMYAAVGYNG ITALQVANRL
560 570 580 590 600
TEKERKQRDQ EEQEKIVQEV TGEPKPYPQG RKREAGVRVK GIDNLLVRLS
610 620 630 640 650
KCCNPVPGDD IVGFITKGRG VSVHREDCPN VKTNEAQERL IPVEWEHESQ
660 670 680 690 700
VQKRKEYNVE IEILGYDRRG LLNEVLQAVN ETKTNISSVS GKSDRNKVAT
710 720 730
IHMAIFIQNI NHLHKVVERI KQIRDIYSVR RVMN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U86377 Genomic DNA Translation: AAC46041.1 AL009126 Genomic DNA Translation: CAB14719.2 |
PIRi | C69691 |
RefSeqi | NP_390638.2, NC_000964.3 WP_003229747.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14719; CAB14719; BSU27600 |
GeneIDi | 51992375 936753 |
KEGGi | bsu:BSU27600 |
PATRICi | fig|224308.179.peg.2999 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U86377 Genomic DNA Translation: AAC46041.1 AL009126 Genomic DNA Translation: CAB14719.2 |
PIRi | C69691 |
RefSeqi | NP_390638.2, NC_000964.3 WP_003229747.1, NZ_JNCM01000036.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6HTQ | electron microscopy | 4.50 | x | 391-731 | [»] | |
SMRi | O54408 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | O54408, 1 interactor |
STRINGi | 224308.BSU27600 |
Proteomic databases
jPOSTi | O54408 |
PaxDbi | O54408 |
PRIDEi | O54408 |
Genome annotation databases
EnsemblBacteriai | CAB14719; CAB14719; BSU27600 |
GeneIDi | 51992375 936753 |
KEGGi | bsu:BSU27600 |
PATRICi | fig|224308.179.peg.2999 |
Phylogenomic databases
eggNOGi | COG0317, Bacteria |
InParanoidi | O54408 |
OMAi | HYYSIYQ |
PhylomeDBi | O54408 |
Enzyme and pathway databases
UniPathwayi | UPA00908;UER00884 |
BioCyci | BSUB:BSU27600-MONOMER |
Family and domain databases
CDDi | cd00077, HDc, 1 hit cd05399, NT_Rel-Spo_like, 1 hit cd01668, TGS_RSH, 1 hit |
Gene3Di | 3.10.20.30, 1 hit 3.30.460.10, 1 hit |
InterProi | View protein in InterPro IPR002912, ACT_dom IPR012675, Beta-grasp_dom_sf IPR003607, HD/PDEase_dom IPR043519, NT_sf IPR004811, RelA/Spo_fam IPR007685, RelA_SpoT IPR004095, TGS IPR012676, TGS-like IPR033655, TGS_RelA |
Pfami | View protein in Pfam PF13291, ACT_4, 1 hit PF04607, RelA_SpoT, 1 hit PF02824, TGS, 1 hit |
SMARTi | View protein in SMART SM00471, HDc, 1 hit SM00954, RelA_SpoT, 1 hit |
SUPFAMi | SSF81271, SSF81271, 1 hit SSF81301, SSF81301, 1 hit |
TIGRFAMsi | TIGR00691, spoT_relA, 1 hit |
PROSITEi | View protein in PROSITE PS51671, ACT, 1 hit PS51831, HD, 1 hit PS51880, TGS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RELA_BACSU | |
Accessioni | O54408Primary (citable) accession number: O54408 Secondary accession number(s): O32043 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | July 7, 2009 | |
Last modified: | December 2, 2020 | |
This is version 146 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families